Background:Green coffee beans contain biochemical compounds including caffeine,chlorogenic acid,trigonelline,and diterpenoid alcohols.In traditional Iranian medicine,coffee bean powder is used for the treatment of wou...Background:Green coffee beans contain biochemical compounds including caffeine,chlorogenic acid,trigonelline,and diterpenoid alcohols.In traditional Iranian medicine,coffee bean powder is used for the treatment of wounds.Previous studies have shown that green coffee bean extract has a number of health benefits,including induction of weight loss,reduced blood pressure,and hepatoprotective and anti-Parkinson effects.The aim of this study was to investigate the wound-healing activity of green coffee bean extract on a full-thickness wound model.Methods:Full-thickness wounds of 20×20 mm were created on the back of New Zealand white rabbits.The animals were divided into 6 groups.Three concentrations of green coffee bean extract(5%,10%,and 15%w/w)in a eucerin base were applied over the wounds in 3 test groups.One percent phenytoin cream was used in one group as a positive control.The rabbits of negative control and vehicle groups received no treatment and eucerin,respectively.For the evaluation of green coffee bean extract’s wound-healing effects,measures included:(1)wound-closure rate,by daily measuring of the wound surface area and calculating the reduction in area;(2)period of epithelialization,the number of days until the scab dropped from the wound;(3)hydroxyproline content,measured by a hydroxyproline assay kit;(4)lipid peroxidation,measured by a lipid peroxidation assay kit;and(5)histopathological state(hematoxylin-eosin stain)of wound-tissue samples on days 7 and 14 post-wounding.Results:The results of this study showed significantly enhanced wound-closure rate,shorter period of epithelialization(P<0.01),increased hydroxyproline content(P<0.001)and suppressed lipid peroxidation(P<0.001 on day 14)of wounds on animals treated with the 10%green coffee bean extract compared to the negative control and eucerin-treated groups.Moreover,the therapeutic effects of 10%green coffee bean were significantly superior than those of phenytoin on enhancing wound-closure rate,decreasing period of epithelialization(P<0.05),increasing hydroxyproline content(P<0.001 on day 7 and P<0.0001 on day 14)and suppressing lipid peroxidation(P<0.0001 on day 14).In addition,histopathological study supported the wound healing activity of green coffee bean extract.Conclusion:Our results showed that green coffee bean extract has a potential for promoting wound healing,thus supporting its traditional use for this purpose.展开更多
In this work,a field-applicable,simple method for quick detecting chlorogenic acid(CGA)was developed.The method is based on CGA’s antioxidant property.CGA is reported to reduce a mixture solution of FeCl_(3)/K_(3)Fe(...In this work,a field-applicable,simple method for quick detecting chlorogenic acid(CGA)was developed.The method is based on CGA’s antioxidant property.CGA is reported to reduce a mixture solution of FeCl_(3)/K_(3)Fe(CN)_(6)to generate Fe_(4)[Fe(CN)_(6)]_(3),the Prussian blue,in an aqueous solution.This reaction was adopted in this work by using solid-ink printed annuli on a filter-paper as reaction vessels.The reagent is pre-loaded and dried within the solid ink printed circles.In a field test,a drop of a sample solution is directly applied to a reagent-loaded circle.The reaction product within the circle shows a blue color,which is detected by using an iPad(or cellphone)camera as a detector and a light-emitting diode flashlight lamp as a light source.A freely available RGB colorimeter program from Apple store was used to read light intensity of three colors:red,green,blue,and absorbance signal of red and blue light is calculated.The difference of absorbance signal of red light from that of blue light was found having linear relationship with CGA concentration in an aqueous sample solution.This simple method is sensitive with a detection limit of 1.0μg/mL for CGA in sample solution.The technique was used to analyze CGA concentration in green coffee extract,and the analytical results agree well with that obtained using a UV/Vis absorption spectrometric method.The real sample test results demonstrate the usefulness of this simple method for quick detecting CGA in green coffee extract products in field test applications.展开更多
基金This study was financially supported by Vice-Chancellor of Research and Technology,Hamadan University of Medical Sciences,Hamadan,Iran(Gran no,9509095210).
文摘Background:Green coffee beans contain biochemical compounds including caffeine,chlorogenic acid,trigonelline,and diterpenoid alcohols.In traditional Iranian medicine,coffee bean powder is used for the treatment of wounds.Previous studies have shown that green coffee bean extract has a number of health benefits,including induction of weight loss,reduced blood pressure,and hepatoprotective and anti-Parkinson effects.The aim of this study was to investigate the wound-healing activity of green coffee bean extract on a full-thickness wound model.Methods:Full-thickness wounds of 20×20 mm were created on the back of New Zealand white rabbits.The animals were divided into 6 groups.Three concentrations of green coffee bean extract(5%,10%,and 15%w/w)in a eucerin base were applied over the wounds in 3 test groups.One percent phenytoin cream was used in one group as a positive control.The rabbits of negative control and vehicle groups received no treatment and eucerin,respectively.For the evaluation of green coffee bean extract’s wound-healing effects,measures included:(1)wound-closure rate,by daily measuring of the wound surface area and calculating the reduction in area;(2)period of epithelialization,the number of days until the scab dropped from the wound;(3)hydroxyproline content,measured by a hydroxyproline assay kit;(4)lipid peroxidation,measured by a lipid peroxidation assay kit;and(5)histopathological state(hematoxylin-eosin stain)of wound-tissue samples on days 7 and 14 post-wounding.Results:The results of this study showed significantly enhanced wound-closure rate,shorter period of epithelialization(P<0.01),increased hydroxyproline content(P<0.001)and suppressed lipid peroxidation(P<0.001 on day 14)of wounds on animals treated with the 10%green coffee bean extract compared to the negative control and eucerin-treated groups.Moreover,the therapeutic effects of 10%green coffee bean were significantly superior than those of phenytoin on enhancing wound-closure rate,decreasing period of epithelialization(P<0.05),increasing hydroxyproline content(P<0.001 on day 7 and P<0.0001 on day 14)and suppressing lipid peroxidation(P<0.0001 on day 14).In addition,histopathological study supported the wound healing activity of green coffee bean extract.Conclusion:Our results showed that green coffee bean extract has a potential for promoting wound healing,thus supporting its traditional use for this purpose.
基金Killgore Research Grant Program of West Texas A&M University for providing fund to purchase the solid ink printer used in this work
文摘In this work,a field-applicable,simple method for quick detecting chlorogenic acid(CGA)was developed.The method is based on CGA’s antioxidant property.CGA is reported to reduce a mixture solution of FeCl_(3)/K_(3)Fe(CN)_(6)to generate Fe_(4)[Fe(CN)_(6)]_(3),the Prussian blue,in an aqueous solution.This reaction was adopted in this work by using solid-ink printed annuli on a filter-paper as reaction vessels.The reagent is pre-loaded and dried within the solid ink printed circles.In a field test,a drop of a sample solution is directly applied to a reagent-loaded circle.The reaction product within the circle shows a blue color,which is detected by using an iPad(or cellphone)camera as a detector and a light-emitting diode flashlight lamp as a light source.A freely available RGB colorimeter program from Apple store was used to read light intensity of three colors:red,green,blue,and absorbance signal of red and blue light is calculated.The difference of absorbance signal of red light from that of blue light was found having linear relationship with CGA concentration in an aqueous sample solution.This simple method is sensitive with a detection limit of 1.0μg/mL for CGA in sample solution.The technique was used to analyze CGA concentration in green coffee extract,and the analytical results agree well with that obtained using a UV/Vis absorption spectrometric method.The real sample test results demonstrate the usefulness of this simple method for quick detecting CGA in green coffee extract products in field test applications.
