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血清lncRNA THRIL、lncRNA NEAT1与新生儿肺炎病情程度及预后的相关性
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作者 刘鑫 张宏蕊 +2 位作者 沈颖 刁玉巧 樊涛 《实用医学杂志》 北大核心 2026年第2期327-333,共7页
目的探究血清长链非编码RNA肿瘤坏死因子相关异种核糖核蛋白L(lncRNA THRIL)、长链非编码RNA核富集转录本1(lncRNA NEAT1)与新生儿肺炎病情程度、预后的关系。方法选取2022年8月至2024年8月河北医科大学第四医院收治的120例新生儿肺炎... 目的探究血清长链非编码RNA肿瘤坏死因子相关异种核糖核蛋白L(lncRNA THRIL)、长链非编码RNA核富集转录本1(lncRNA NEAT1)与新生儿肺炎病情程度、预后的关系。方法选取2022年8月至2024年8月河北医科大学第四医院收治的120例新生儿肺炎患儿为观察组,根据病情程度分为轻症组(42例)、中症组(40例)和重症组(38例);根据治疗2周后预后情况分为预后良好组(86例)和预后不良组(34例)。同时,选取同期在医院进行健康体检的120例健康新生儿,将其设为对照组。采用实时荧光定量PCR法测定受试新生儿血清lncRNA THRIL、lncRNA NEAT1水平;收集新生儿肺炎患儿临床资料,并检测免疫炎症指标[血清可溶性髓样细胞触发受体-1(sTREM-1)、可溶性白细胞介素2受体(sIL-2R)]。对于新生儿肺炎患儿预后不良的影响因素,采用logistic回归分析进行识别与验证;针对血清lncRNA THRIL和lncRNA NEAT1对患儿不良预后的预测作用,通过受试者工作特征(ROC)曲线分析予以评价,明确两者单独及联合预测的临床价值。结果观察组血清lncRNA THRIL、lncRNA NEAT1水平与对照组相比显著升高(P<0.05);血清lncRNA THRIL、lncRNA NEAT1水平随着新生儿肺炎病情的加重而逐渐升高(P<0.05);与预后良好组相比,预后不良组剖腹产占比、血清sTREM-1、sIL-2R、lncRNA THRIL、lncRNA NEAT1水平均显著升高(P<0.05);血清sIL-2R、lncRNA THRIL、lncRNA NEAT1为新生儿肺炎患儿预后不良的独立危险因素(P<0.05);血清lncRNA THRIL、lncRNA NEAT1、二者联合预测新生儿肺炎患儿发生预后不良的曲线下面积(AUC)分别为0.772、0.808、0.930,二者联合预测的AUC显著高于各指标单独预测的AUC(Z二者联合-lncRNA THRIL=2.347、Z二者联合-lncRNA NEAT1=2.217,P=0.019、0.027)。结论新生儿肺炎患儿血清lncRNA THRIL、lncRNA NEAT1水平均明显升高,二者均是新生儿肺炎预后不良的危险因素,二者联合对新生儿肺炎患儿的预后有较好的预测效果。 展开更多
关键词 新生儿肺炎 长链非编码RNA肿瘤坏死因子相关异种核糖核蛋白L 长链非编码RNA核富集转录本1 病情程度 预后
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lncRNA FGD5-AS1靶向miR-512-3p/RAB31抑制膀胱癌细胞增殖、侵袭和上皮-间质转化
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作者 李富博 李爱科 +5 位作者 饶井芬 刘宝兴 石方玉 李文鑫 杨春丽 林萍萍 《中国医科大学学报》 北大核心 2026年第1期33-40,共8页
目的探讨长链非编码RNA(lncRNA)FGD5反义RNA 1(FGD5-AS1)、miR-512-3p和Ras相关蛋白31(RAB31)在膀胱癌进展中的作用和调控机制。方法收集2019年1月至2021年12月于承德医学院附属医院行手术治疗的60例膀胱癌患者肿瘤组织及癌旁组织,并体... 目的探讨长链非编码RNA(lncRNA)FGD5反义RNA 1(FGD5-AS1)、miR-512-3p和Ras相关蛋白31(RAB31)在膀胱癌进展中的作用和调控机制。方法收集2019年1月至2021年12月于承德医学院附属医院行手术治疗的60例膀胱癌患者肿瘤组织及癌旁组织,并体外培养膀胱癌细胞系(5637、KU-19-19、T24、UM-UC-3)和正常尿路上皮细胞系(SV-HUC-1)。采用实时定量PCR检测肿瘤组织和癌旁组织以及膀胱癌细胞中FGD5-AS1、miR-512-3p和RAB31 mRNA表达,Pearson相关分析确定膀胱癌患者癌组织中miR-512-3p与FGD5-AS1、RAB31 mRNA表达之间的相关性。将sh-NC、sh-FGD5-AS1、sh-FGD5-AS1和NC抑制剂、sh-FGD5-AS1和miR-512-3p抑制剂转染至T24细胞中,分别记为阴性对照组、FGD5-AS1沉默组、抑制剂对照组、联合组;另设置正常组(不转染)。用CCK-8法检测细胞活力;Transwell小室测定细胞迁移和侵袭能力;Western blotting检测RAB31和E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(vimentin)表达;双萤光素酶报告基因和RNA Pull down实验检测miR-512-3p与FGD5-AS1和RAB31的靶向关系。结果膀胱癌组织与细胞中FGD5-AS1、RAB31 mRNA呈高表达,miR-512-3p呈低表达(P<0.05),且膀胱癌患者癌组织中FGD5-AS1、RAB31 m RNA的表达与mi R-512-3p表达呈负相关,FGD5-AS1与RAB31 mRNA表达呈正相关(r=-0.779、-0.649、0.652,均P<0.001)。沉默FGD5-AS1可上调miR-512-3p表达,下调RAB31 mRNA和蛋白表达,降低细胞活力、迁移和侵袭数以及N-cadherin、vimentin水平,升高E-cadherin水平(P<0.05);敲低miR-512-3p表达可明显减弱沉默FGD5-AS1对膀胱癌T24细胞增殖、迁移和侵袭以及上皮-间质转化(EMT)进程的抑制作用(P<0.05);FGD5-AS1可以海绵化miR-512-3p,而RAB31是miR-512-3p的靶标。结论沉默FGD5-AS1可能通过上调miR-512-3p、下调RAB31表达抑制膀胱癌细胞的增殖、迁移、侵袭和EMT进程。 展开更多
关键词 膀胱癌 增殖 上皮-间质转化 长链非编码RNA FGD5-AS1 miR-512-3p/Ras相关蛋白31
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Tunable reflective spin-decoupled encoding metasurface based on Dirac semimetals
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作者 HAO Xiao-yu ZHENG Si-yu +6 位作者 WANG Yu LIU Yang LIU Meng ZHANG Yu-ping ZHANG Jin-juan ZHAN Yi ZHANG Hui-yun 《中国光学(中英文)》 北大核心 2025年第4期968-978,共11页
Multiple functional metasurfaces with high information capacity have attracted considerable attention from researchers.This study proposes a 2-bit tunable spin-decoupled coded metasurface designed for the terahertz ba... Multiple functional metasurfaces with high information capacity have attracted considerable attention from researchers.This study proposes a 2-bit tunable spin-decoupled coded metasurface designed for the terahertz band,which utilizes the tunable properties of Dirac semimetals(DSM)to create a novel multilayer structure.By incorporating both geometric and propagating phases into the metasurface design,we can effectively control the electromagnetic wave.When the Fermi level(EF)of the DSM is set at 6 meV,the electromagnetic wave is manipulated by the gold patch embedded in the DSM film,operating at a frequency of 1.3 THz.When the EF of the DSM is set at 80 meV,the electromagnetic wave is manipulated by the DSM patch,operating at a frequency of 1.4 THz.Both modes enable independent control of beam splitting under left-rotating circularly polarized(LCP)and rightrotating circularly polarized(RCP)wave excitation,resulting in the generation of vortex beams with distinct orbital angular momentum(OAM)modes.The findings of this study hold significant potential for enhancing information capacity and polarization multiplexing techniques in wireless communications. 展开更多
关键词 coding metasurface dirac semimetal spin decoupling circular polarization TUNABLE
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哮喘患者血清lncRNA HOTTIP和RMRP水平表达及与气道重塑的相关性分析
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作者 郁芳芳 谢伟国 许群 《现代检验医学杂志》 2026年第1期70-74,85,共6页
目的探究哮喘患者血清长链非编码RNA(lncRNA)HOXA末端转录本反义RNA(HOTTIP)和线粒体RNA处理核糖核酸内切酶RNA组份(RMRP)表达变化及其与气道重塑的相关性。方法选取2022年1月~2024年6月江苏省江阴市人民医院呼吸与危重症医学科收治的... 目的探究哮喘患者血清长链非编码RNA(lncRNA)HOXA末端转录本反义RNA(HOTTIP)和线粒体RNA处理核糖核酸内切酶RNA组份(RMRP)表达变化及其与气道重塑的相关性。方法选取2022年1月~2024年6月江苏省江阴市人民医院呼吸与危重症医学科收治的急性发作期哮喘患者72例为急性发作期组,同期门诊随访哮喘缓解期患者70例为缓解期组,健康体检者142例为健康对照组。采用实时荧光定量聚合酶链反应(qRT-PCR)检测血清lncRNA HOTTIP和RMRP水平。采用多排螺旋CT测量患者肺内气道的内径(L)、外径(D),计算气道壁厚度与气道外径比值(T/D)和管壁面积占支气道总横截面积百分比(WA%)。采用肺功能检测仪检测第一秒用力呼气量(FEV1)、最高呼吸气流(PEF)水平。Spearman相关性分析哮喘患者血清lncRNA HOTTIP和RMRP水平与哮喘控制测试(ACT)评分的关系。Pearson相关性分析哮喘患者血清lncRNA HOTTIP和RMRP水平以及与肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)、白细胞介素-18(IL-18)水平、气道重塑以及肺功能指标的关系。结果与健康对照组相比,急性发作期组和缓解期组IFN-γ水平显著降低(t=14.161,8.886),TNF-α、IL-18、lncRNA HOTTIP(1.32±0.15、1.15±0.15 vs 1.01±0.14)和RMRP(1.27±0.14、1.16±0.14 vs 1.02±0.13)水平显著升高(t=9.345~28.361);与缓解期组相比,急性发作期组ACT评分、IFN-γ水平显著降低(t=13.344,4.475),TNF-α、IL-18、lncRNA HOTTIP和RMRP水平显著升高(t=6.861~15.675),差异具有统计学意义(均P<0.05)。与健康对照组相比,急性发作期组和缓解期组FEV1、PEF水平显著降低(t=13.346~55.694),T/D、WA%水平显著升高(t=19.145~33.035);与缓解期组相比,急性发作期组FEV1、PEF水平显著降低(t=21.802、21.446),T/D、WA%水平显著升高(t=9.994、10.082),差异具有统计学意义(均P<0.05)。Spearman相关性分析显示哮喘患者血清lncRNA HOTTIP和RMRP水平与ACT评分呈负相关(r=-0.614、-0.399,均P<0.001)。Pearson相关性分析显示,哮喘患者血清lncRNA HOTTIP和RMRP水平呈正相关(r=0.625,P<0.05)。哮喘患者血清lncRNA HOTTIP和RMRP水平与TNF-α(r=0.658、0.632)、IL-18(r=0.584、0.586)、T/D(r=0.604、0.631)、WA%(r=0.597、0.588)水平呈正相关,与IFN-γ(r=-0.587、-0.517)、FEV1(r=-0.568、-0.577)、PEF(r=-0.634、-0.627)水平呈负相关(均P<0.05)。结论哮喘患者血清lncRNA HOTTIP和RMRP水平均呈高表达,与气道重塑和肺功能指标变化有关。 展开更多
关键词 哮喘 长链非编码核糖核酸HOXA末端转录本反义RNA 线粒体RNA处理核糖核酸内切酶RNA组份 气道重塑
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Broadband polarization-independent terahertz multifunctional liquid crystal coding metasurface based on topological optimization
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作者 Yu Chen Wu-Hao Cao +4 位作者 Jia-Qi Li Ming-Zhe Zhang Xin-Yi Du Ding-Shan Gao Pei-Li Li 《Chinese Physics B》 2025年第4期432-440,共9页
A broadband polarization-independent terahertz multifunctional coding metasurface based on topological optimization using liquid crystal(LC)is proposed.The metasurface can achieve reconfigurability for beam steering a... A broadband polarization-independent terahertz multifunctional coding metasurface based on topological optimization using liquid crystal(LC)is proposed.The metasurface can achieve reconfigurability for beam steering and vortex beam generation within a frequency range of 0.68 THz–0.72 THz.Firstly,the metasurface unit is topologically optimized using the non-dominant sequencing genetic algorithms(NSGA-II)multi-objective optimization algorithm.By applying the LC’s electrically tunable refractive index properties,the metasurface unit enables polarization-independent 2-bit coding within a frequency range of 0.68 THz–0.72 THz.Then,based on the designed metasurface unit,the array arrangement of the metasurface is reverse-designed to achieve beam steering and vortex beam generation.The results show that,for beam steering,not only can polarization-independent steering of both single-and multi-beam be achieved within the 35°elevation angle range,but also independent control of the target angle of each beam in the multi-beam steering.For vortex beam generation,the metasurfaces can achieve the generation of single-and multi-vortex beams with topological charges l=±1,±2 within the 35elevation angle range,and the generation angles of each vortex beam in the multi-vortex beam can be independently controlled.This provides flexibility and diversity in the generation of vortex beams.Therefore,the proposed terahertz LC metasurface can realize flexible control of reconfigurable functions and has certain application prospects in terahertz communication,phased array radar,and vortex radar. 展开更多
关键词 coding metasurfaces polarization-independent TERAHERTZ topology optimization
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Review for wireless communication technology based on digital encoding metasurfaces
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作者 Haojie Zhan Manna Gu +10 位作者 Ying Tian Huizhen Feng Mingmin Zhu Haomiao Zhou Yongxing Jin Ying Tang Chenxia Li Bo Fang Zhi Hong Xufeng Jing Le Wang 《Opto-Electronic Advances》 2025年第7期51-106,共56页
Metasurfaces offer exceptional capabilities for controlling electromagnetic waves,enabling the realization of unique electromagnetic properties.As communication technology continues to evolve,metasurfaces present prom... Metasurfaces offer exceptional capabilities for controlling electromagnetic waves,enabling the realization of unique electromagnetic properties.As communication technology continues to evolve,metasurfaces present promising applications in wireless communications.This paper reviews the latest advancements in metasurface research within the communication sector,explores metasurface-based wireless relay technologies,and summarizes various wireless communication methods employing different types of metasurfaces across diverse modulation schemes.This paper provides a detailed discussion on the design of wireless communication systems based on coding metasurfaces to simplify transmitter architecture,as well as the development of intelligent coding metasurfaces in the communication field.It also elaborates on the application of vector vortex light fields in metasurface communication.Finally,it offers a forward-looking perspective on wireless communication systems that incorporate coded metasurfaces.This review aims to furnish researchers with a thorough understanding of the current state and future directions of coded metasurface applications in communications. 展开更多
关键词 coding metasurface RIS wireless communications signal modulation TRANSMITTER vortex light
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A graph neural network and multi-task learning-based decoding algorithm for enhancing XZZX code stability in biased noise
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作者 Bo Xiao Zai-Xu Fan +2 位作者 Hui-Qian Sun Hong-Yang Ma Xing-Kui Fan 《Chinese Physics B》 2025年第5期250-257,共8页
Quantum error correction is a technique that enhances a system’s ability to combat noise by encoding logical information into additional quantum bits,which plays a key role in building practical quantum computers.The... Quantum error correction is a technique that enhances a system’s ability to combat noise by encoding logical information into additional quantum bits,which plays a key role in building practical quantum computers.The XZZX surface code,with only one stabilizer generator on each face,demonstrates significant application potential under biased noise.However,the existing minimum weight perfect matching(MWPM)algorithm has high computational complexity and lacks flexibility in large-scale systems.Therefore,this paper proposes a decoding method that combines graph neural networks(GNN)with multi-classifiers,the syndrome is transformed into an undirected graph,and the features are aggregated by convolutional layers,providing a more efficient and accurate decoding strategy.In the experiments,we evaluated the performance of the XZZX code under different biased noise conditions(bias=1,20,200)and different code distances(d=3,5,7,9,11).The experimental results show that under low bias noise(bias=1),the GNN decoder achieves a threshold of 0.18386,an improvement of approximately 19.12%compared to the MWPM decoder.Under high bias noise(bias=200),the GNN decoder reaches a threshold of 0.40542,improving by approximately 20.76%,overcoming the limitations of the conventional decoder.They demonstrate that the GNN decoding method exhibits superior performance and has broad application potential in the error correction of XZZX code. 展开更多
关键词 quantum error correction XZZX code biased noise graph neural network
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Low Complexity Successive Cancellation List Decoding of U-UV Codes
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作者 Chen Wenhao Chen Li +1 位作者 Lin Jingyu Zhang Huazi 《China Communications》 2025年第1期41-60,共20页
Constituted by BCH component codes and its ordered statistics decoding(OSD),the successive cancellation list(SCL)decoding of U-UV structural codes can provide competent error-correction performance in the short-to-med... Constituted by BCH component codes and its ordered statistics decoding(OSD),the successive cancellation list(SCL)decoding of U-UV structural codes can provide competent error-correction performance in the short-to-medium length regime.However,this list decoding complexity becomes formidable as the decoding output list size increases.This is primarily incurred by the OSD.Addressing this challenge,this paper proposes the low complexity SCL decoding through reducing the complexity of component code decoding,and pruning the redundant SCL decoding paths.For the former,an efficient skipping rule is introduced for the OSD so that the higher order decoding can be skipped when they are not possible to provide a more likely codeword candidate.It is further extended to the OSD variant,the box-andmatch algorithm(BMA),in facilitating the component code decoding.Moreover,through estimating the correlation distance lower bounds(CDLBs)of the component code decoding outputs,a path pruning(PP)-SCL decoding is proposed to further facilitate the decoding of U-UV codes.In particular,its integration with the improved OSD and BMA is discussed.Simulation results show that significant complexity reduction can be achieved.Consequently,the U-UV codes can outperform the cyclic redundancy check(CRC)-polar codes with a similar decoding complexity. 展开更多
关键词 ordered statistics decoding successive cancellation list decoding U-UV codes
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Adjustable random linear network coding(ARLNC): A solution for data transmission in dynamic IoT computational environments
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作者 Raffi Dilanchian Ali Bohlooli Kamal Jamshidi 《Digital Communications and Networks》 2025年第2期574-586,共13页
In mobile computing environments, most IoT devices connected to networks experience variable error rates and possess limited bandwidth. The conventional method of retransmitting lost information during transmission, c... In mobile computing environments, most IoT devices connected to networks experience variable error rates and possess limited bandwidth. The conventional method of retransmitting lost information during transmission, commonly used in data transmission protocols, increases transmission delay and consumes excessive bandwidth. To overcome this issue, forward error correction techniques, e.g., Random Linear Network Coding(RLNC) can be used in data transmission. The primary challenge in RLNC-based methodologies is sustaining a consistent coding ratio during data transmission, leading to notable bandwidth usage and transmission delay in dynamic network conditions. Therefore, this study proposes a new block-based RLNC strategy known as Adjustable RLNC(ARLNC), which dynamically adjusts the coding ratio and transmission window during runtime based on the estimated network error rate calculated via receiver feedback. The calculations in this approach are performed using a Galois field with the order of 256. Furthermore, we assessed ARLNC's performance by subjecting it to various error models such as Gilbert Elliott, exponential, and constant rates and compared it with the standard RLNC. The results show that dynamically adjusting the coding ratio and transmission window size based on network conditions significantly enhances network throughput and reduces total transmission delay in most scenarios. In contrast to the conventional RLNC method employing a fixed coding ratio, the presented approach has demonstrated significant enhancements, resulting in a 73% decrease in transmission delay and a 4 times augmentation in throughput. However, in dynamic computational environments, ARLNC generally incurs higher computational costs than the standard RLNC but excels in high-performance networks. 展开更多
关键词 Random linear network coding Adjust redundancy Galois field Internet of Things Data transfer
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LncRNA-14238/TLR-7信号通路参与腰椎间盘突出症痛觉高敏的机制研究
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作者 张磊 白明生 喻新祥 《颈腰痛杂志》 2026年第1期13-18,共6页
目的 探讨长链非编码核糖核酸-14238(LncRNA-14238)/Toll样受体-7(TLR-7)信号通路参与腰椎间盘突出症(LDH)痛觉高敏的机制。方法 68只雄性Sprague-Dawley大鼠分为正常组、LDH组和小干扰长链非编码核糖核酸(si LncRNA)组,采用自体髓核植... 目的 探讨长链非编码核糖核酸-14238(LncRNA-14238)/Toll样受体-7(TLR-7)信号通路参与腰椎间盘突出症(LDH)痛觉高敏的机制。方法 68只雄性Sprague-Dawley大鼠分为正常组、LDH组和小干扰长链非编码核糖核酸(si LncRNA)组,采用自体髓核植入左侧L5/L6神经根旁构建LDH模型,正常组仅行手术操作但不植入髓核,高通量测序检测LncRNA-14238的表达,此外,通过疼痛行为测试、承重实验和旋转杆分析检测LDH模型的痛觉高敏反应,实时荧光定量聚合酶链反应(qRT-PCR)和蛋白质印迹检测LncRNA-14238、TLR-7和内源性酶胱硫氨酸-β合成酶(CBS)的表达。结果 与正常组相比,LncRNA-14238、TLR-7和CBS在LDH组中表达升高,si LncRNA组的LncRNA-14238、TLR-7和CBS表达降低;LDH组的痛觉高敏反应增加,而沉默LncRNA-14238降低了LDH组的痛觉高敏反应,差异有统计学意义(P<0.05)。结论 LncRNA-14238/TLR-7信号通路可能参与LDH痛觉高敏反应。 展开更多
关键词 长链非编码核糖核酸-14238 Toll样受体-7 腰椎间盘突出症 痛觉高敏反应 内源性酶胱硫氨酸-β合成酶 高通量测序
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Correction: Silencing of the long non-coding RNA LINC00265triggers autophagy and apoptosis in lung cancer by reducingprotein stability of SIN3A oncogene
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作者 XIAOBI HUANG CHUNYUAN CHEN +9 位作者 YONGYANG CHEN HONGLIAN ZHOU YONGHUA CHEN ZHONG HUANG YULIU XIE BAIYANG LIU YUDONG GUO ZHIXIONG YANG GUANGHUA CHEN WENMEI SU 《Oncology Research》 2025年第5期1249-1250,共2页
In the article“Silencing of the long non-coding RNA LINC00265 triggers autophagy and apoptosis in lung cancer by reducing protein stability of SIN3A oncogene”(Oncology Research.2024,Vol.32,No.7,pp.1185–1195.doi:10.... In the article“Silencing of the long non-coding RNA LINC00265 triggers autophagy and apoptosis in lung cancer by reducing protein stability of SIN3A oncogene”(Oncology Research.2024,Vol.32,No.7,pp.1185–1195.doi:10.32604/or.2023.030771,https://www.techscience.com/or/v32n7/57163),an inadvertent error occurred during the compilation of Fig.3H.This needed corrections to ensure the accuracy and integrity of the data presented. 展开更多
关键词 lung cancer long non coding RNA reducing protein stability sin oncogene oncology AUTOPHAGY protein stability APOPTOSIS accuracy integrity SILEncING
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CUDA‑based GPU‑only computation for efficient tracking simulation of single and multi‑bunch collective effects
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作者 Keon Hee Kim Eun‑San Kim 《Nuclear Science and Techniques》 2026年第1期61-79,共19页
Beam-tracking simulations have been extensively utilized in the study of collective beam instabilities in circular accelerators.Traditionally,many simulation codes have relied on central processing unit(CPU)-based met... Beam-tracking simulations have been extensively utilized in the study of collective beam instabilities in circular accelerators.Traditionally,many simulation codes have relied on central processing unit(CPU)-based methods,tracking on a single CPU core,or parallelizing the computation across multiple cores via the message passing interface(MPI).Although these approaches work well for single-bunch tracking,scaling them to multiple bunches significantly increases the computational load,which often necessitates the use of a dedicated multi-CPU cluster.To address this challenge,alternative methods leveraging General-Purpose computing on Graphics Processing Units(GPGPU)have been proposed,enabling tracking studies on a standalone desktop personal computer(PC).However,frequent CPU-GPU interactions,including data transfers and synchronization operations during tracking,can introduce communication overheads,potentially reducing the overall effectiveness of GPU-based computations.In this study,we propose a novel approach that eliminates this overhead by performing the entire tracking simulation process exclusively on the GPU,thereby enabling the simultaneous processing of all bunches and their macro-particles.Specifically,we introduce MBTRACK2-CUDA,a Compute Unified Device Architecture(CUDA)ported version of MBTRACK2,which facilitates efficient tracking of single-and multi-bunch collective effects by leveraging the full GPU-resident computation. 展开更多
关键词 Code development GPU computing Collective effects
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LncRNA KCNQ1OT1调节miR-218-5p/KLF8轴对肺癌细胞增殖、凋亡和上皮间质转化的影响
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作者 易宣洪 黄晓智 +2 位作者 李志梁 熊伟 王静 《临床肺科杂志》 2026年第2期250-256,共7页
目的探讨长链非编码RNA(LncRNA)KCNQ1OT1调节微小RNA(miR)-218-5p/Krüppel样因子8(KLF8)轴对人肺癌细胞增殖、凋亡和上皮间质转化的影响。方法将A549细胞分为对照(Control)、si-NC、si-KC1、si-KC1+miR-In-NC、si-KC1+miR-218-5p-I... 目的探讨长链非编码RNA(LncRNA)KCNQ1OT1调节微小RNA(miR)-218-5p/Krüppel样因子8(KLF8)轴对人肺癌细胞增殖、凋亡和上皮间质转化的影响。方法将A549细胞分为对照(Control)、si-NC、si-KC1、si-KC1+miR-In-NC、si-KC1+miR-218-5p-In组。qRT-PCR实验检测细胞中LncRNA KCNQ1OT1、miR-218-5p和KLF8 mRNA水平;CCK-8法检测细胞增殖;Annexin V/PI染色法检测细胞凋亡;划痕愈合和Transwell实验检测细胞迁移和侵袭;Western Blot检测细胞中KLF8、E-cadherin、N-cadherin和Bax水平;双荧光素酶实验测定miR-218-5p与LncRNA KCNQ1OT1间的靶向关系。结果与si-NC组相比,si-KC1组细胞存活率、划痕愈合率、细胞侵袭数、LncRNA KCNQ1OT1、KLF8 mRNA以及KLF8、N-cadherin蛋白水平下降,凋亡率、miR-218-5p、Bax和E-cadherin表达上升(P<0.05);与si-KC1+miR-In-NC组相比,si-KC1+miR-218-5p-In组细胞存活率、划痕愈合率、细胞侵袭数、KLF8 mRNA以及KLF8、N-cadherin蛋白水平显著升高(P<0.05),凋亡率、miR-218-5p、E-cadherin和Bax水平显著降低(P<0.05),LncRNA KCNQ1OT1差异无统计学意义(P>0.05);与miR-NC组相比,共转染KC1-WT和miR-218-5p mimic降低细胞中荧光酶活性(P<0.05)。结论敲低LncRNA KCNQ1OT1可能经由miR-218-5p/KLF8轴,诱导人肺癌细胞凋亡,并抑制上皮间质转化和增殖。 展开更多
关键词 长链非编码RNA KCNQ1OT1 微小RNA-218-5p/Krüppel样因子8轴 人肺癌细胞
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血清lncRNA CRNDE和miR-378表达与急性缺血性脑卒中患者神经功能损伤的相关性研究
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作者 吴宜泽 冼冠秀 +3 位作者 宋岱鸿 黄丹彤 吴南昌 罗宏成 《国际检验医学杂志》 2026年第1期42-47,53,共7页
目的探讨急性缺血性脑卒中(AIS)患者血清中长链非编码RNA(lncRNA)CRNDE和微小RNA(miR)-378的表达与神经功能损伤之间的关系。方法选取2023年5月至2024年11月右江民族医学院附属医院神经内科108例AIS住院患者作为AIS组,108例同期体检健... 目的探讨急性缺血性脑卒中(AIS)患者血清中长链非编码RNA(lncRNA)CRNDE和微小RNA(miR)-378的表达与神经功能损伤之间的关系。方法选取2023年5月至2024年11月右江民族医学院附属医院神经内科108例AIS住院患者作为AIS组,108例同期体检健康者作为健康组。根据美国国立卫生研究院脑卒中量表(NIHSS)评分将AIS患者分为损伤组(31例)和非损伤组(77例)。采用实时荧光定量PCR分析所有受试者血清lncRNA CRNDE和miR-378相对表达水平,比较健康组和AIS组血清lncRNA CRNDE、miR-378相对表达水平。采用Pearson秩相关分析lncRNA CRNDE、miR-378相对表达水平与NIHSS评分之间的相关性,采用多因素Logistic回归分析探讨AIS患者合并神经功能损伤的影响因素,采用受试者工作特征(ROC)曲线评估lncRNA CRNDE和miR-378相对表达水平对AIS患者合并神经功能损伤的诊断价值。结果与健康组相比,AIS组血清lncRNA CRNDE相对表达水平升高(t=0.089,P<0.001),miR-378相对表达水平降低(t=17.270,P<0.001);Spearman相关分析显示,AIS患者血清中lncRNA CRNDE相对表达水平与NIHSS评分呈正相关(r=0.393,P<0.001),miR-378相对表达水平与NIHSS评分呈负相关(r=-0.467,P<0.001);损伤组白细胞、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、入院时改良Rankin量表(mRS)评分、lncRNA CRNDE水平均较非损伤组更高(t/χ^(2)=3.269、2.433、2.873、3.456、7.526、4.06,P<0.05),miR-378相对表达水平低于非损伤组(t=-4.738,P<0.05);多因素Logistic回归分析结果显示,低密度脂蛋白胆固醇、入院时mRS评分、lncRNA CRNDE及miR-378均是AIS患者合并神经功能损伤的独立危险因素(OR=0.029、0.104、0.009,P<0.05);lncRNA CRNDE和miR-378诊断AIS患者合并神经功能损伤的曲线下面积(AUC)分别为0.826(95%CI:0.737~0.914,P<0.001)、0.753(95%CI:0.648~0.858,P<0.001),灵敏度分别为83.9%、67.7%,特异度分别为75.3%、72.7%;lncRNA CRNDE和miR-378联合诊断AIS患者合并神经功能损伤的AUC为0.896(95%CI:0.835~0.957,P<0.001),灵敏度为87.1%,特异度为80.5%。结论AIS患者血清中lncRNA CRNDE相对表达水平升高,miR-378相对表达水平降低,二者与AIS患者神经功能损伤密切相关,可作为辅助诊断AIS患者合并神经功能损伤的血清标志物,且二者联合诊断效能更高。 展开更多
关键词 急性缺血性脑卒中 神经功能损伤 长链非编码RNA CRNDE 微小RNA-378
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宫颈癌组织LncRNA HOTTIP表达及其与临床病理特征、生存预后的关系分析
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作者 马晓明 闫谨 马煜磊 《医学理论与实践》 2026年第1期26-30,47,共6页
目的:探讨宫颈癌组织长链非编码核糖核酸(LncRNA)同源异形盒A远端转录本(HOTTIP)表达及其与临床病理特征、生存预后的关系。方法:选取2019年2月—2023年4月我院145例宫颈癌患者为研究对象。收集手术切除的宫颈癌组织及癌旁组织,采用实... 目的:探讨宫颈癌组织长链非编码核糖核酸(LncRNA)同源异形盒A远端转录本(HOTTIP)表达及其与临床病理特征、生存预后的关系。方法:选取2019年2月—2023年4月我院145例宫颈癌患者为研究对象。收集手术切除的宫颈癌组织及癌旁组织,采用实时荧光定量聚合酶链反应(RT-qPCR)法检测LncRNA HOTTIP相对表达量并比较。比较不同临床病理特征患者癌组织LncRNA HOTTIP相对表达量。自出院当天持续随访至2024年4月或死亡,按照生存预后情况分为生存组、死亡组,比较2组临床资料,采用Cox回归法分析生存预后的影响因素,并进行Kaplan-Meier生存分析。结果:共脱落10例,最终纳入135例;宫颈癌组织LncRNA HOTTIP相对表达量高于癌旁组织(P<0.05);相较于病理类型鳞癌、国际妇产科联盟(FIGO)Ⅰ~Ⅱ期、肿瘤中/高分化、无神经侵犯及无脉管癌栓患者,病理类型非鳞癌、FIGOⅢ~Ⅳ期、肿瘤低/未分化、神经侵犯及脉管癌栓患者的癌组织LncRNA HOTTIP相对表达量升高(P<0.05);随访9~58个月,中位数39(20,46)个月,死亡率为38.52%(52/135);FIGOⅢ~Ⅳ期、肿瘤低/未分化、神经侵犯、脉管癌栓及癌组织LncRNA HOTTIP高表达是宫颈癌患者死亡的独立危险因素(P<0.05);Kaplan-Meier生存分析显示,FIGOⅢ~Ⅳ期、肿瘤低/未分化、神经侵犯、脉管癌栓及癌组织LncRNA HOTTIP高表达患者的生存率低于FIGOⅠ~Ⅱ期、肿瘤中/高分化、无神经侵犯、无脉管癌栓及癌组织LncRNA HOTTIP低表达患者(P<0.05)。结论:宫颈癌患者癌组织LncRNA HOTTIP相对表达量升高,癌组织LncRNA HOTTIP相对表达量不仅与病理类型、FIGO分期、肿瘤分化程度、神经侵犯及脉管癌栓密切相关,且为生存预后的影响因素。 展开更多
关键词 宫颈癌 长链非编码核糖核酸 同源异形盒A远端转录本 临床病理特征 生存预后
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lncRNA GAS6-AS1调节miR-326/E2F1轴对神经母细胞瘤细胞增殖、迁移和侵袭影响的实验研究
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作者 安艳晓 祁艳卫 仲智勇 《现代检验医学杂志》 2026年第1期46-50,69,共6页
目的探究长链非编码RNA(lncRNA)生长阻滞特异性基因6反义RNA1(GAS6-AS1)通过调节微小RNA-326(miR-326)/E2F转录因子1(E2F1)轴对神经母细胞瘤(NB)细胞增殖、迁移和侵袭的影响。方法收集23例2021年8月~2023年1月在河北省儿童医院接受治疗... 目的探究长链非编码RNA(lncRNA)生长阻滞特异性基因6反义RNA1(GAS6-AS1)通过调节微小RNA-326(miR-326)/E2F转录因子1(E2F1)轴对神经母细胞瘤(NB)细胞增殖、迁移和侵袭的影响。方法收集23例2021年8月~2023年1月在河北省儿童医院接受治疗的NB患儿瘤组织及瘤旁组织,实时荧光定量聚合酶链反应(qRT-PCR)检测NB组织和细胞中lncRNA GAS6-AS1、miR-326、E2F1 mRNA表达水平并用双荧光素酶实验检测三者间的关系。将SK-N-SH细胞随机分为对照(control)组、短发夹RNA阴性对照(sh-NC)组、GAS6-AS1短发夹RNA(sh-GAS6-AS1)组、sh-GAS6-AS1+抑制剂阴性对照(anti-NC)组、sh-GAS6-AS1+miR-326抑制剂(anti-miR-326)组。qRT-PCR检测各组细胞中lncRNA GAS6-AS1和miR-326表达水平,克隆形成实验、划痕实验、Transwell实验及蛋白免疫印迹法分别检测NB细胞增殖、迁移、侵袭及E2F1、细胞周期蛋白D1(Cyclin D1)、基质金属蛋白酶(MMP)-2、MMP-9蛋白表达;构建裸鼠移植瘤模型,随机将小鼠分为sh-NC组和sh-GAS6-AS1组,qRT-PCR检测移植瘤组织中lncRNA GAS6-AS1和miR-326水平,免疫组化检测移植瘤组织中E2F1蛋白表达。结果与瘤旁组织和人正常神经细胞视网膜色素上皮细胞D-407比较,NB瘤组织和SK-N-SH细胞中lncRNA GAS6-AS1水平、E2F1 mRNA表达升高,miR-326表达降低,差异具有统计学意义(t=7.221~53.271,均P<0.05);lncRNA GAS6-AS1及E2F1与miR-326存在靶向关系。与sh-NC组比较,sh-GAS6-AS1组细胞克隆形成数目、划痕愈合率、侵袭细胞数目、lncRNA GAS6-AS1、E2F1、Cyclin D1、MMP-2、MMP-9表达下降,miR-326表达上升,差异具有统计学意义(q=8.706~16.489,均P<0.05)。与sh-GAS6-AS1+anti-NC组比较,sh-GAS6-AS1+anti-miR-326组克隆形成数目、划痕愈合率、侵袭细胞数目、E2F1、Cyclin D1、MMP-2、MMP-9表达升高,miR-326表达降低,差异具有统计学意义(q=4.173~13.407,均P<0.05)。裸鼠实验显示,与sh-NC组比较,sh-GAS6-AS1组肿瘤质量、体积均下降,lncRNA GAS6-AS1、E2F1表达下调,miR-326表达上调,差异具有统计学意义(t=8.684~13.494,均P<0.05)。结论抑制lncRNA GAS6-AS1表达可能通过靶向miR-326/E2F1轴抑制NB细胞增殖、迁移、侵袭。 展开更多
关键词 神经母细胞瘤 长链非编码RNA生长阻滞特异性基因6反义RNA1 微小RNA-326 E2F转录因子1 增殖 迁移 侵袭
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升麻素苷调控lncRNA CIR改善IL-1β诱导的软骨细胞外基质稳态失衡的研究
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作者 林琴 陈长兴 李超 《中医康复》 2026年第1期24-29,共6页
目的:观察升麻素苷通过调节lncRNA CIR改善IL-1β诱导的软骨细胞外基质(Extracellular Matrix,ECM)稳态失衡的作用。方法:分离与收集C57BL/6小鼠膝关节软骨组织,进行软骨细胞的体外培养。将培养好的软骨细胞随机分组并予以相应干预后,采... 目的:观察升麻素苷通过调节lncRNA CIR改善IL-1β诱导的软骨细胞外基质(Extracellular Matrix,ECM)稳态失衡的作用。方法:分离与收集C57BL/6小鼠膝关节软骨组织,进行软骨细胞的体外培养。将培养好的软骨细胞随机分组并予以相应干预后,采用Real-time PCR检测升麻素苷干预后小鼠软骨细胞中lncRNA CIR水平变化;Western blot检测小鼠软骨细胞中MMP-9、MMP-13、ADAMTS-5和Caspase-3蛋白含量变化;免疫荧光检测小鼠软骨细胞中MMP-13、Caspase-3荧光强度情况。结果:与模型组比较,升麻素苷(POG)组中lncRNA CIR水平显著降低(P<0.05),POG组中MMP-9、MMP-13、ADAMTS-5和Caspase-3蛋白含量显著降低(P<0.05),POG组MMP-13、Caspase-3的荧光强度降低。结论:POG可改善IL-1β诱导的软骨细胞ECM稳态异常,与调控lncRNA CIR、MMP-9、MMP-13、ADAMTS-5和Caspase-3表达有关。 展开更多
关键词 升麻素苷 软骨细胞 骨关节炎 非编码长链RNA CIR
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ADVANCED FREQUENCY-DIRECTED RUN-LENTH BASED CODING SCHEME ON TEST DATA COMPRESSION FOR SYSTEM-ON-CHIP 被引量:1
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作者 张颖 吴宁 葛芬 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2012年第1期77-83,共7页
Test data compression and test resource partitioning (TRP) are essential to reduce the amount of test data in system-on-chip testing. A novel variable-to-variable-length compression codes is designed as advanced fre... Test data compression and test resource partitioning (TRP) are essential to reduce the amount of test data in system-on-chip testing. A novel variable-to-variable-length compression codes is designed as advanced fre- quency-directed run-length (AFDR) codes. Different [rom frequency-directed run-length (FDR) codes, AFDR encodes both 0- and 1-runs and uses the same codes to the equal length runs. It also modifies the codes for 00 and 11 to improve the compression performance. Experimental results for ISCAS 89 benchmark circuits show that AFDR codes achieve higher compression ratio than FDR and other compression codes. 展开更多
关键词 test data compression FDR codes test resource partitioning SYSTEM-ON-CHIP
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lncRNA XIST调控miR-17-5p/FOSL1轴对胆囊癌细胞增殖、迁移和侵袭的影响
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作者 陈李康 姚磊 彭莹莹 《肝胆胰外科杂志》 2026年第2期115-123,共9页
目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证l... 目的探讨长链非编码RNA(lncRNA)X染色体失活特异性转录本(XIST)调控微小RNA-17-5p(miR-17-5p)/FOS样抗原1(FOSL1)轴对胆囊癌(GBC)细胞增殖、迁移和侵袭的影响。方法qRT-PCR法检测GBC组织和细胞株中mRNA表达;双荧光素酶报告基因实验验证lncRNA XIST与miR-17-5p,miR-17-5p与FOSL1的互作;将EH-GB1细胞分为Ctrl组、sh-NC组、sh-XIST组、sh-XIST+inhibitor-NC组、sh-XIST+inhibitor-miR-17-5p组、mimic-NC组、mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组、mimic-miR-17-5p+OE-FOSL1组。台盼蓝染色和平板克隆实验检测EH-GB1细胞增殖;划痕试验检测EH-GB1细胞迁移;Transwell实验检测EH-GB1细胞侵袭;Western blotting检测EH-GB1细胞中Ki67、Cyclin D1、MMP-2、CD44、FOSL1蛋白的表达。裸鼠移植瘤实验检测敲低lncRNA XIST对GBC肿瘤生长的影响。结果GBC组织和细胞中miR-17-5p呈低表达,lncRNA XIST、FOSL1呈高表达。sh-XIST组细胞存活率、增殖数、划痕愈合率、侵袭数,Ki67、Cyclin D1、MMP-2、CD44表达水平低于sh-NC组、Ctrl组(P<0.05);sh-XIST+inhibitor-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44表达水平高于sh-XIST组、sh-XIST+inhibitor-NC组(P<0.05)。mimic-miR-17-5p组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平低于mimic-NC组、Ctrl组(P<0.05);mimic-miR-17-5p+OE-FOSL1组细胞存活率、增殖数、划痕愈合率、侵袭数及Ki67、Cyclin D1、MMP-2、CD44、FOSL1表达水平高于mimic-miR-17-5p组、mimic-miR-17-5p+OE-NC组(P<0.05)。lncRNA XIST可以靶向负调控miR-17-5p,而miR-17-5p可以靶向负调控FOSL1(P<0.05)。XIST敲低组裸鼠移植瘤体积、体质量,肿瘤FOSL1蛋白、lncRNA XIST表达水平低于阴性对照组,miR-17-5p表达水平高于阴性对照组(P<0.05)。结论lncRNA XIST通过调控miR-17-5p/FOSL1轴促进GBC细胞的增殖、迁移和侵袭。 展开更多
关键词 长链非编码RNA X染色体失活特异性转录本 微小RNA-17-5p FOS样抗原1 胆囊癌 增殖 迁移 侵袭
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Performance of STBC-MC-CDMA system based on complex wavelet packet and turbo coding
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作者 虞湘宾 毕光国 《Journal of Southeast University(English Edition)》 EI CAS 2005年第4期398-404,共7页
On the basis of analyzing the principle of the space-time coding technique and the multi-carrier code division multiple access (MC-CDMA) technique, adopting the turbo codes as channel coding and the optimized comple... On the basis of analyzing the principle of the space-time coding technique and the multi-carrier code division multiple access (MC-CDMA) technique, adopting the turbo codes as channel coding and the optimized complex wavelet packet as multi-carrier modulation, a novel space-time block coded the MC-CDMA system based on complex wavelet packet and turbo coding is proposed, and the system bit error rate (BER) performance in the Rayleigh fading channel is investigated. The system can make full use of space-time block codes' transmit diversity and turbo codes' good ability against fading channel to improve the BER performance significantly, and it can also avoid the decrease of spectrum efficiency of conventional MC-CDMA due to inserting cyclic prefix (CP) by utilizing superior characteristics of the optimized complex wavelet packet. Simulation results show that the proposed space-time block coded MC-CDMA system based on the complex wavelet packet performs better than the conventional space-time block coded MC-CDMA (STBC-MC-CDMA) system, and slightly outperforms the STBC-MC-CDMA with CP. Moreover, the application of the space-time block coding technique concatenated with turbo codes strengthens the system ability to combat various interferences in fading channel further. 展开更多
关键词 multi-carrier technique complex wavelet packet space-time block code code division multiple access turbo coding
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