[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materia...[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materials in order to investigate and analyze leaf SPAD value, relative water content （RWC）, malonaldehyde （MAD）, cell membrane permeability （RP）, proline （PRO） and superoxide dismutase activity （SOD）. [Result] As drought stress improved, leaf SPAD value and RWC were both diminishing and RWC fell remarkably; MAD, Pro, RP and SOD activity were increasing; MAD and RP grew significantly. [Conclusion] Kadsura coccinea is much sensitive to drought stress and will be affected if water loss is too much high.展开更多
A new triterpenoid,possessing a lanost-9（11）-3-one skeleton,was isolated from the roots of Kadsura coccinea,based on the spectral analysis,its structure was elucidated as 3-hydroxy-12-acetoxycoccinic acid.
Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of ani...Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of animals were divided each containing six animals.Two wound models including incision and excision wound models were used in this study.The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone(NFZ) ointment(0.2%w/w). Six extracts(ethanol.aqueous,petroleum ether,benzene,chloroform and ethyl acetate ) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains riz. Staphylococcia aureus.Bacillus pumilius,Enterococcus facialis.Escherichia coli,Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griscofulvin for antifungal screening.The serial dilution and cup(or) well plate methods were used for the antimicrobial study and MIC was determined.Results:The ethanolic extract showed significant (P【0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group.Amongst all.ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard.The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study. Conclusions:The ethanolic root extract of I.coccinea showed pronounced wound healing and antibacterial activity.The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.展开更多
AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. The...AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS. RESULTS: Two compounds were determined as(7′S,8′S,8R)-(8β,8′α)- dimethyl-4,4′-dihydroxy-5,3′-dimethoxy-5′-cyclolignan glucoside(1) and micrandiactone H(2), respectively. CONCLUSION: Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide(NO) production in lipopolysaccharide-induced RAW264.7 macrophages.展开更多
Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spe...Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spectroscopic data.Additionally,the structure of 1 was ambiguously verifed by single-crystal X-ray difraction,while the structure of 2,which features a novel 8,16-epoxy motif,was validated by quantum chemical calculation of NMR parameters and ECD spectrum.Moreover,1 and 4 were found to exhibited anticoagulant activity,while 3 and 4 were found to possess anti-platelet aggregation activity.展开更多
The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chrom...The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.展开更多
[Objective] The aim was to study the growth and quality characteristics of sun-cured tobacco Kadsura Coccinea in Guangchang County and give full play to its advantages. [Method] The research took tobacco varieties inc...[Objective] The aim was to study the growth and quality characteristics of sun-cured tobacco Kadsura Coccinea in Guangchang County and give full play to its advantages. [Method] The research took tobacco varieties including Tiechi in Yiqian, Mushao in Yangxi Tiechi in Chishui, and Liuye in Toupo as test materials to explore development process, quality characteristics and economic traits of different varieties. [Result] The variety of Tiechi in Yiqian had medium growht, moderate growth periods, and the best economic benefits. Total content of aroma matter was the highest in the varieties of Tiechi in Yiqian. Also it had more content of products of browning reaction and products degraded by cembranoids. Besides, volume and quality of aroma were higher, as well as the score of sensory quality. [Conclusion]Comprehensive comparisons suggested that the variety of Tiechi in Yiqian was superior to others at tobacco-growing, economic benefits and quality.展开更多
[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rat...[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.展开更多
Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural ...Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural and configurational determination was accomplished through extensive spectroscopic analysis coupled with quantum chemical calculations.Kadcoccitanes E-H were tested for their cytotoxic activities against five human tumor cell lines(HL-60,A-549,SMMC-7721,MDA-MB-231,SW-480)but none of them exhibited activities at the concentration 40μM.展开更多
Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by...Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by biological method. Highly stable and spherical zinc oxide nanoparticles are produced by using zinc acetate and Ixora coccinea leaf extract. Formation of zinc oxide nanoparticles has been confirmed by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering analysis (DLS), zetapotential study and Scanning Electron Microscope with the Energy Dispersive X-ray studies (EDX). Dynamic light scattering analysis shows average particle size of 145.1 nm whereas high zeta potential value confirms the stability of formed zinc oxide nanoparticles. The Scanning Electron Microscope reveals spherical morphology of nanoparticles and Energy Dispersive X-ray analysis confirms the formation of highly pure zinc oxide nanoparticles. The zinc oxide nanoparticles from Ixora coccinea leaves are expected to have applications in biomedical, cosmetic industries, biotechnology, sensors, medical, catalysis, optical device, coatings, drug delivery and water remediation, and also may be applied for electronic and magneto-electric devices. This new eco-friendly approach of synthesis is a novel, cheap, and convenient technique suitable for large scale commercial production.展开更多
[Objectives]The purpose was to provide scientific basis for the identification and application of Kadsura coccinea and its chief adulterant Kadsura heteroclite.[Methods]The botanical characteristics,traits and microsc...[Objectives]The purpose was to provide scientific basis for the identification and application of Kadsura coccinea and its chief adulterant Kadsura heteroclite.[Methods]The botanical characteristics,traits and microscopic morphology of the two plants were compared.[Results]K.coccinea was clearly distinguished from K.heteroclite in botanical characteristics,traits and microscopic morphology.[Conclusions]This study is of great significance to the identification and development of K.coccinea.展开更多
Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is ...Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is considered a biowaste.This significantly restricts the development of the K.coccinea fruit industry.Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K.coccinea.Herein,we evaluated K.coccinea fruit peel,pulp,and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes.The most prominent metabolite classes included lipids,amino acids,flavonoids,and lignans.Furthermore,our results emphasized a significant accumulation of flavonoids in pulp tissues,while alkaloids and lignans were abundant in peel and seed tissues,respectively.A total of 183 metabolites were differentially accumulated among the three tissues.Procyanidin C2,rutinoside,2-hydroxyoleanolic acid,5-hydroxymethyluracil,nootkatol,isoquercitrin,isohyperoside,quercetin-7-O-glucoside,hyperin,and rutin showed elevated accumulation in the peel.In the seed,kadsuralignan G,kadcoccilactone A,kadsuralignan H,lysoPE 20:5,iso-schisandrin ethyl alcohol,and kadangustin were significantly enriched.Our results highlight the diverse metabolome composition of K.coccinea fruit parts,which can be further exploited for its valorization in various industries.展开更多
[Objectives]This study was conducted to investigate the diurnal variations of photosynthetic and physiological characteristics in 3-year-old Kadsura coccinea(Lem.)A.C.Smith plants.[Methods]A Li-6400 portable photosynt...[Objectives]This study was conducted to investigate the diurnal variations of photosynthetic and physiological characteristics in 3-year-old Kadsura coccinea(Lem.)A.C.Smith plants.[Methods]A Li-6400 portable photosynthetic system was used to study the diurnal variations of the photosynthetic physiological characteristics of K.coccinea.[Results]The diurnal variation of the net photosynthetic rate(Pn)of K.coccinea leaves showed a"double peak"curve,and there was a lunch break at noon.The maximum value of Pn[4.51 mmol/(m^(2)·s)]appeared at 10:00,and the daily average Pn was 3.98 mol/(m^(2)·s);and the stepwise regression analysis showed that photosynthetically active radiation(PAR)and stomatal conductance(Gs)were the main factors affecting Pn.The partial correlation analysis and path analysis showed that the order of effects on Pn was Gs>PAR.[Conclusions]This study provides a reference for further research on the development and utilization of K.coccinea.展开更多
The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect ...The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect the industrial development of rare and multi-purpose wild fruit Kadsura coccinea(Lem.)A.C.Smith.Through the investigation of K.coccinea production area and planting area,collection and analysis of fruit samples,it was found that in most planting area,the varieties of K.coccinea were mixed,with great differences in fruit quality and yield,which makes it impossible to realize large-scale planting.In view of these problems,a series of studies have been carried out and the technical regulations for standardized planting of rare and multi-purpose wild fruit K.coccinea were formulated.展开更多
Pain treatment is one of the most challenging situations of the modern medicine. To overcome the actual limitations, new strategies should be developed and phytotherapy represents a promising alternative. <em>Bo...Pain treatment is one of the most challenging situations of the modern medicine. To overcome the actual limitations, new strategies should be developed and phytotherapy represents a promising alternative. <em>Boerhavia coccinea</em> is a medicinal plant used for the treatment of pain. The present work was undertaken to evaluate the antinociceptive effects of crude aqueous extract of the leaves of <em>Boerhavia coccinea</em> (AE) on acute pain and examine its mechanism of action. The analgesic effect of AE was evaluated at doses 50, 100, 200 and 400 mg/kg using the formalin-induced nociception in mice. The specific analgesic effect of AE was verified by testing its effect of AE on the sleep induced by diazepam. The anti-inflammatory effects of AE were also tested <em>in vivo</em> (100 and 200 mg/kg) on CFA-induced inflammation and in vitro (1 to 300 <em>μ</em>g/ml) on the production of NO by non-activated or activated (LPS, 1 <em>μ</em>g/l) macrophages. The effect of AE in non-stimulated macrophages was evaluated in absence and in presence of L-NAME (100 <em>μ</em>g/ml) or dexathetasone (30 <em>μ</em>g/ml). AE administered orally significantly (<em>p</em> < 0.001) inhibited both neurogenic and inflammatory phases of the formalin pain with a maximum inhibition percentage of 81% at the 1<sup>st</sup> phase and 90% at the 2<sup>nd</sup> phase, but did not show any anti-inflammatory effect. AE at 50 mg/kg increased the latency to sleep and reduced sleep duration. AE drastically (<em>p</em> < 0.001) increased the NO production by non-activated or activated macrophages by up to 5654%. L-NAME and dexamethasone potentiated the activation effect of AE on NO production. In conclusion, AE possess potent antinociceptive effect that is not related to any anti-inflammatory activity. Instead, this extract increases the nitric oxide production by an unknown mechanism.展开更多
Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and...Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at R<sub>f</sub>0.55.展开更多
The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflam...The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of MEOC （500 mg/kg） produced significant （t7 〈 0.05） antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that MEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of MEOC showed maximum inhibition （64.29%） at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that MEOC has a peripheral antinoeiceptive and anti-inflammatory action.展开更多
基金Supported by Guangxi Science Research and Technology Development Planning Program(11107010-1-411107010-1-4-1)~~
文摘[Objective] The aim was to research drought resistance and adaptation mechanisms of Kaclsura coccinea under different drought stresses. [Method] The seedlings of Kadsura coccinea growing one year were taken as materials in order to investigate and analyze leaf SPAD value, relative water content （RWC）, malonaldehyde （MAD）, cell membrane permeability （RP）, proline （PRO） and superoxide dismutase activity （SOD）. [Result] As drought stress improved, leaf SPAD value and RWC were both diminishing and RWC fell remarkably; MAD, Pro, RP and SOD activity were increasing; MAD and RP grew significantly. [Conclusion] Kadsura coccinea is much sensitive to drought stress and will be affected if water loss is too much high.
文摘A new triterpenoid,possessing a lanost-9（11）-3-one skeleton,was isolated from the roots of Kadsura coccinea,based on the spectral analysis,its structure was elucidated as 3-hydroxy-12-acetoxycoccinic acid.
文摘Objective:To evaluate the wound healing and antimicrobial activity of root extracts of Ixora coccinea(I.coccinea).Methods:To investigate the wound healing efficacy of root extract of I.coccinea Linn,five groups of animals were divided each containing six animals.Two wound models including incision and excision wound models were used in this study.The parameters studied were tensile strength on incision wound model and in terms of wound contraction for excision wound model were compared with standard Nitrofurazone(NFZ) ointment(0.2%w/w). Six extracts(ethanol.aqueous,petroleum ether,benzene,chloroform and ethyl acetate ) of I. coccinea were screened for in vitro growth inhibiting activity against different bacterial strains riz. Staphylococcia aureus.Bacillus pumilius,Enterococcus facialis.Escherichia coli,Salmonella typhi and Pseudomonas aeruginosa and fungi Candida albicans and Aspergillus niger were compared with the standard drugs ciprofloxacin and chloramphenicol for antibacterial and griscofulvin for antifungal screening.The serial dilution and cup(or) well plate methods were used for the antimicrobial study and MIC was determined.Results:The ethanolic extract showed significant (P【0.001) wound healing activity when compared to standard drug NFZ with respect to normal control group.Amongst all.ethanolic extract showed highly significant antibacterial activity against all bacterial strains used in this study when compared to standard.The aqueous extract showed moderate significant inhibition against all bacterial strains when compared to standard. All the extracts were shown negligible activity against the fungal strains used in this study. Conclusions:The ethanolic root extract of I.coccinea showed pronounced wound healing and antibacterial activity.The probable reason to heal the wound was that the external application of the extract prevented the microbes to invade through the wound thus the protection of wound occurs against the infection of the various organisms.
基金supported by the Program for New Century Excellent Talents in University(NCET-12-0977)the National New Drug Innovation Great Project of China(2011ZX09307-002-02)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in a University(PCSIRT-IRT1193)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)
文摘AIM: To study the chemical constituents of the roots and stem bark of Kadsura coccinea. METHOD: Compounds were isolated by column chromatography on silica gel and Sephadex LH-20, and finally purified by prep-HPLC. Their structures were elucidated by extensive spectroscopic methods, including 1D- and 2D-NMR, and HR-ESI-MS. RESULTS: Two compounds were determined as(7′S,8′S,8R)-(8β,8′α)- dimethyl-4,4′-dihydroxy-5,3′-dimethoxy-5′-cyclolignan glucoside(1) and micrandiactone H(2), respectively. CONCLUSION: Compunds 1 and 2 are new and neither showed inhibitory effects on nitric oxide(NO) production in lipopolysaccharide-induced RAW264.7 macrophages.
基金supported financially by the National Natural Science Foundation of China(Nos.81874298 and 81673329).
文摘Four new rearranged 6/6/5/6-fused lanostane-type triterpenoids,kadcoccitanes A-D(1-4),were isolated from the roots of Kadsura coccinea,and their structures were mainly elucidated by comprehensive analysis of their spectroscopic data.Additionally,the structure of 1 was ambiguously verifed by single-crystal X-ray difraction,while the structure of 2,which features a novel 8,16-epoxy motif,was validated by quantum chemical calculation of NMR parameters and ECD spectrum.Moreover,1 and 4 were found to exhibited anticoagulant activity,while 3 and 4 were found to possess anti-platelet aggregation activity.
基金supported by the PAPD(Priority Academic Program Development)of Jiangsu Higher Education Institutions,University Science Research Project of Jiangsu Province(No.09KJB350002)National Key Technology R&D Program(No.2012BAI30B02),National Natural Science Foundation of China(No.30901853)Hepatic Fibrosis Innovative Medicine-Research and Development of Fructus Schisandrae capsule(No.F12-157-9-00)
文摘The stereochemistry of two 6, 9-oxygen bridge dibenzocyclooctadiene lignans from Kadsura coccinea, are difficult to separate and very unstable. The present study was designed to develop a high-performance liquid chromatography using circular dichroism detection for the analysis of the stereochemistry. A new 6, 9-oxygen bridge dibenzocyclooctadiene lignans named Kadsulignan Q was firstly found with an S-biphenyl configuration. The other compound was identified as Kadsulignan L with an R- biphenyl configuration. In order to obtain kinetic data on their reversible interconversion, the stability was measured at different deuterated solvents such as deuterated methanol, deuterated chloroform and deuterated dimethylsulfoxide. The lignans were more unstable and converted more easily in deuterated methanol than in deuterated chloroform and deuterated dimethylsulfoxide.
基金Supported by Key Technology of"Kadsura Coccinea"of China Tobacco(Guangchang County,Jiangxi Province)(201205)~~
文摘[Objective] The aim was to study the growth and quality characteristics of sun-cured tobacco Kadsura Coccinea in Guangchang County and give full play to its advantages. [Method] The research took tobacco varieties including Tiechi in Yiqian, Mushao in Yangxi Tiechi in Chishui, and Liuye in Toupo as test materials to explore development process, quality characteristics and economic traits of different varieties. [Result] The variety of Tiechi in Yiqian had medium growht, moderate growth periods, and the best economic benefits. Total content of aroma matter was the highest in the varieties of Tiechi in Yiqian. Also it had more content of products of browning reaction and products degraded by cembranoids. Besides, volume and quality of aroma were higher, as well as the score of sensory quality. [Conclusion]Comprehensive comparisons suggested that the variety of Tiechi in Yiqian was superior to others at tobacco-growing, economic benefits and quality.
基金Supported by National Natural Science Foundation of China(No.81303162)China Postdoctoral Science Foundation(No.2015T80784,2014M552017)Postdoctoral Scientific Research Project of Henan Province(No.2013010)
文摘[Objectives] To investigate the therapeutic effect of Kadsura coccinea on adjuvant arthritis( AA) rats and its mechanism from cytokine levels. [Methods] The complete Freund's adjuvant( CFA)-induced AA model of rats was used. The therapeutic effect of K. coccinea on AA rats was evaluated by the articular swelling,arthritis score,immune organ index and hematoxylin-eosin( HE) staining. The content of inflammatory factors TNF-α,IL-6,and IL-1β in the serum of AA rats was detected by enzyme-linked immunosorbent assay( ELISA) respectively. [Results]Compared with the model group,the total extract of K. coccinea( KCTE) could significantly inhibit the primary and secondary articular swelling of AA rats,lower the arthritis score and immune organ index,and improve the pathological state of ankles of AA rats. In the groups with a high and middle dosage of KCTE( KCTE-H and KCTE-M),KCTE could significantly reduce the content of TNF-α and IL-6 in the serum of rats,exhibiting a certain dose-effect relationship. [Conclusions]KCTE had significant therapeutic effect on the AA of rats,and the mechanism might be associated with the modulation of immune function and the decrease in the secretion of inflammatory cytokines TNF-α and IL-6.
基金Natural Science Foundation of Yunnan Province(202101AT070188).
文摘Four new 14(13→12)-abeolanostane triterpenoids featuring extendedπ-conjugated systems,kadcoccitanes E-H(1-4),were obtained from the stems of Kadsura coccinea through using a HPLC−UV-guided approach.Their structural and configurational determination was accomplished through extensive spectroscopic analysis coupled with quantum chemical calculations.Kadcoccitanes E-H were tested for their cytotoxic activities against five human tumor cell lines(HL-60,A-549,SMMC-7721,MDA-MB-231,SW-480)but none of them exhibited activities at the concentration 40μM.
文摘Green synthesis of metal oxide nanoparticles using plant extract is a promising alternative to traditional method of chemical synthesis. In this paper, we report the synthesis of nanostructured zinc oxide particles by biological method. Highly stable and spherical zinc oxide nanoparticles are produced by using zinc acetate and Ixora coccinea leaf extract. Formation of zinc oxide nanoparticles has been confirmed by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Dynamic light scattering analysis (DLS), zetapotential study and Scanning Electron Microscope with the Energy Dispersive X-ray studies (EDX). Dynamic light scattering analysis shows average particle size of 145.1 nm whereas high zeta potential value confirms the stability of formed zinc oxide nanoparticles. The Scanning Electron Microscope reveals spherical morphology of nanoparticles and Energy Dispersive X-ray analysis confirms the formation of highly pure zinc oxide nanoparticles. The zinc oxide nanoparticles from Ixora coccinea leaves are expected to have applications in biomedical, cosmetic industries, biotechnology, sensors, medical, catalysis, optical device, coatings, drug delivery and water remediation, and also may be applied for electronic and magneto-electric devices. This new eco-friendly approach of synthesis is a novel, cheap, and convenient technique suitable for large scale commercial production.
基金Special Grant for Public Health Services in the Chinese Medicine Sector(GXZYZYPC17-3)Guangxi-Australia Chinese Medicine Quality Research Joint Laboratory Construction(Gui Ke AD17195002).
文摘[Objectives]The purpose was to provide scientific basis for the identification and application of Kadsura coccinea and its chief adulterant Kadsura heteroclite.[Methods]The botanical characteristics,traits and microscopic morphology of the two plants were compared.[Results]K.coccinea was clearly distinguished from K.heteroclite in botanical characteristics,traits and microscopic morphology.[Conclusions]This study is of great significance to the identification and development of K.coccinea.
文摘Kadsura coccinea(Lem.)is a woody wine plant with a peculiar fruit enriched in important health-promoting compounds.The non-editable part of the fruit,i.e.,the seed and peel,represents more than 60%of the fruit and is considered a biowaste.This significantly restricts the development of the K.coccinea fruit industry.Clarifying the metabolic components of the different fruit parts can help to improve the utilization rate and valorization of K.coccinea.Herein,we evaluated K.coccinea fruit peel,pulp,and seed using widely-targeted metabolomics and quantified a set of 736 bioactive compounds from 11 major metabolite classes.The most prominent metabolite classes included lipids,amino acids,flavonoids,and lignans.Furthermore,our results emphasized a significant accumulation of flavonoids in pulp tissues,while alkaloids and lignans were abundant in peel and seed tissues,respectively.A total of 183 metabolites were differentially accumulated among the three tissues.Procyanidin C2,rutinoside,2-hydroxyoleanolic acid,5-hydroxymethyluracil,nootkatol,isoquercitrin,isohyperoside,quercetin-7-O-glucoside,hyperin,and rutin showed elevated accumulation in the peel.In the seed,kadsuralignan G,kadcoccilactone A,kadsuralignan H,lysoPE 20:5,iso-schisandrin ethyl alcohol,and kadangustin were significantly enriched.Our results highlight the diverse metabolome composition of K.coccinea fruit parts,which can be further exploited for its valorization in various industries.
基金Supported by Guangxi Key R&D Program Project(GKAB18221091)Guilin Scientific Research and Planning Science and Technology Key Project(20160223-1)。
文摘[Objectives]This study was conducted to investigate the diurnal variations of photosynthetic and physiological characteristics in 3-year-old Kadsura coccinea(Lem.)A.C.Smith plants.[Methods]A Li-6400 portable photosynthetic system was used to study the diurnal variations of the photosynthetic physiological characteristics of K.coccinea.[Results]The diurnal variation of the net photosynthetic rate(Pn)of K.coccinea leaves showed a"double peak"curve,and there was a lunch break at noon.The maximum value of Pn[4.51 mmol/(m^(2)·s)]appeared at 10:00,and the daily average Pn was 3.98 mol/(m^(2)·s);and the stepwise regression analysis showed that photosynthetically active radiation(PAR)and stomatal conductance(Gs)were the main factors affecting Pn.The partial correlation analysis and path analysis showed that the order of effects on Pn was Gs>PAR.[Conclusions]This study provides a reference for further research on the development and utilization of K.coccinea.
基金Key R&D projects in Guangxi Zhuang Autonomous Region(GK AB18221091)Scientific Research and Scientific Planning of Guilin,Guangxi(20160223-1).
文摘The cultivation technology were standardized for improving the yield and quality,and the commodity value and the competitiveness of fruit market,and solving the bottleneck problem—fruit quality and yield that affect the industrial development of rare and multi-purpose wild fruit Kadsura coccinea(Lem.)A.C.Smith.Through the investigation of K.coccinea production area and planting area,collection and analysis of fruit samples,it was found that in most planting area,the varieties of K.coccinea were mixed,with great differences in fruit quality and yield,which makes it impossible to realize large-scale planting.In view of these problems,a series of studies have been carried out and the technical regulations for standardized planting of rare and multi-purpose wild fruit K.coccinea were formulated.
文摘Pain treatment is one of the most challenging situations of the modern medicine. To overcome the actual limitations, new strategies should be developed and phytotherapy represents a promising alternative. <em>Boerhavia coccinea</em> is a medicinal plant used for the treatment of pain. The present work was undertaken to evaluate the antinociceptive effects of crude aqueous extract of the leaves of <em>Boerhavia coccinea</em> (AE) on acute pain and examine its mechanism of action. The analgesic effect of AE was evaluated at doses 50, 100, 200 and 400 mg/kg using the formalin-induced nociception in mice. The specific analgesic effect of AE was verified by testing its effect of AE on the sleep induced by diazepam. The anti-inflammatory effects of AE were also tested <em>in vivo</em> (100 and 200 mg/kg) on CFA-induced inflammation and in vitro (1 to 300 <em>μ</em>g/ml) on the production of NO by non-activated or activated (LPS, 1 <em>μ</em>g/l) macrophages. The effect of AE in non-stimulated macrophages was evaluated in absence and in presence of L-NAME (100 <em>μ</em>g/ml) or dexathetasone (30 <em>μ</em>g/ml). AE administered orally significantly (<em>p</em> < 0.001) inhibited both neurogenic and inflammatory phases of the formalin pain with a maximum inhibition percentage of 81% at the 1<sup>st</sup> phase and 90% at the 2<sup>nd</sup> phase, but did not show any anti-inflammatory effect. AE at 50 mg/kg increased the latency to sleep and reduced sleep duration. AE drastically (<em>p</em> < 0.001) increased the NO production by non-activated or activated macrophages by up to 5654%. L-NAME and dexamethasone potentiated the activation effect of AE on NO production. In conclusion, AE possess potent antinociceptive effect that is not related to any anti-inflammatory activity. Instead, this extract increases the nitric oxide production by an unknown mechanism.
文摘Bacterial infection is considered to be one of the most critical health issues of the world. It is essential to overcome this problem by the development of new drugs. Marine organisms as corals, sponges, seaweeds, and other are an incredible source of novel pharmacologically active compounds. Herein, the antimicrobial activity (extract/fractions) of the invasive stony coralTubastraea coccinea was evaluated by the disk diffusion method against 21 microbial strains (ATCC and clinical strains). Micro broth dilution was used to determinate the MIC of the fractions that showed antimicrobial activity by the disk diffusion method. Bioautography assay was also performed. Our results showed that the n-butanol (BF) and aqueous fractions (AF) showed activity against ATCC strains Staphylococcus aureus (MIC 31.25 and 250 μg/mL),Salmonella typhimurium (MIC 125 and 500 μg/mL), Escherichia coli (MIC 62.5 and 500 μg/mL) and Pseudomonas aeruginosa (MIC 62.5 and 500 μg/mL), respectively. Moreover, BF fraction was also effective against the clinical strains S. aureus(MIC 62.5 μg/mL), Klebsiella pneumoniae Carbapenemase—KPC (MIC 125 μg/mL), Methicillin-Resistant Staphylococcus aureus—MRSA (MIC 125 μg/mL) and Vancomycin-Resistant Enterococcus faecalis—VRE (MIC 62.5 μg/mL). The ratio MBC/MIC reinforces the bactericidal profile of BF fraction. The bioautography assay of BF fraction showed the presence of antimicrobial components at R<sub>f</sub>0.55.
文摘The present study was conducted to evaluate the antinociceptive and anti-inflammatory activities of methanol extract of rachis of Ormosia coccinea （Aubl.） Jacks （MEOC） using animal models of nociception and inflammation. The antinociceptive activity of the extract was assessed using acetic acid-induced abdominal writhing, hot-plate, and formalin tests. Oral administration of MEOC （500 mg/kg） produced significant （t7 〈 0.05） antinociceptive effects when tested in mice using acetic acid-induced abdominal writhing test and on the inflammatory phase of the formalin test. It was also demonstrated that MEOC had no significant effect on the response latency time to the heat stimulus in the thermal model of the hot plate test. The anti-inflammatory activity of the extract was assessed using carrageenan, histamine and serotonin induced oedema in rat paw. The oral administration of MEOC showed maximum inhibition （64.29%） at 1 h on carrageenan edema, but it did not modify the edema induced by histamine and serotonin. The present results suggest that MEOC has a peripheral antinoeiceptive and anti-inflammatory action.
基金This study was supported in part by the United States National Science Foundation (NSF) PEET grant DEB-0731510‘Monographic Studies in the Nectriaceae,Hypocreales:Nectria,Cosmospora,and Neonectria' to University of Maryland
