Microbial fuel cells(MFcs)provide a promising solution forsustainable power generation and simultaneous wastewatertreatment.One of the central roles in the applicationadvances of MFCs is to propel the innovation of ef...Microbial fuel cells(MFcs)provide a promising solution forsustainable power generation and simultaneous wastewatertreatment.One of the central roles in the applicationadvances of MFCs is to propel the innovation of efficientand renewable oxygen reduction reaction(ORR)bioelectro-catalysts in air-cathode.Herein,a surface co-engineeredliving cell bioelectrocatalyst was demonstrated through thecoupling strategy of genetic engineering and nanomaterialsengineering,aiming at rationally regulating the ORRcatalytic function of the whole-cell and the bioelectricitygeneration capacity:First,Escherichia coli(E.colf)cells weregenetically programmed to display a mass of bilirubin oxi-dase(BoD)on the cell surface,enabling plentiful splendidcatalytic activity sites for ORR.Further,cell surface encapsu-lation with conductive polypyrrole(PPy)nanomaterials wasestablished,contributing to the enhancement of the con-ductivity of the cell membrane and promotion of the elec-tron transfer efficiency of the abiotic-biotic interface.Besides,the PPy materials formed a tight electron transportnetwork around the BOD active sites,achieving a synergistic catalytic effect.The designed co-engineered E.coli cellsexerted superior electrocatalytic ORR performance with amaximum current density of 3.1mA cm^(-2) and onset potentialof o.61 V.Additionally,the maximum power output of MFCSassembled with this living cell air-cathode biocatalystreached 188.7 uW cm^(-2).Our results not only provide a newavenue to regulate fuel cell activity and function for adesirable chemical reaction but also demonstrate the plas-ticity of the cell surface engineering system.展开更多
基金supported by the National ScienceFoundation of China (grant nos.52271176 and 52472200)the 111Project,China(grant no.D17007)+1 种基金Henan Center for OutstandingOverseas Scientists,China(grant no.GzS2022017)the KeySpecialized Research and Development-International scienceand Technology Cooperation Program in Henan Province,China(grant no.231111520500).
文摘Microbial fuel cells(MFcs)provide a promising solution forsustainable power generation and simultaneous wastewatertreatment.One of the central roles in the applicationadvances of MFCs is to propel the innovation of efficientand renewable oxygen reduction reaction(ORR)bioelectro-catalysts in air-cathode.Herein,a surface co-engineeredliving cell bioelectrocatalyst was demonstrated through thecoupling strategy of genetic engineering and nanomaterialsengineering,aiming at rationally regulating the ORRcatalytic function of the whole-cell and the bioelectricitygeneration capacity:First,Escherichia coli(E.colf)cells weregenetically programmed to display a mass of bilirubin oxi-dase(BoD)on the cell surface,enabling plentiful splendidcatalytic activity sites for ORR.Further,cell surface encapsu-lation with conductive polypyrrole(PPy)nanomaterials wasestablished,contributing to the enhancement of the con-ductivity of the cell membrane and promotion of the elec-tron transfer efficiency of the abiotic-biotic interface.Besides,the PPy materials formed a tight electron transportnetwork around the BOD active sites,achieving a synergistic catalytic effect.The designed co-engineered E.coli cellsexerted superior electrocatalytic ORR performance with amaximum current density of 3.1mA cm^(-2) and onset potentialof o.61 V.Additionally,the maximum power output of MFCSassembled with this living cell air-cathode biocatalystreached 188.7 uW cm^(-2).Our results not only provide a newavenue to regulate fuel cell activity and function for adesirable chemical reaction but also demonstrate the plas-ticity of the cell surface engineering system.
