Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a...Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.展开更多
本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效...本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效应、竞争力效应、交叉效应都推动了我国对RCEP国家纺织品的出口,其中结构效应的贡献最高,交叉效应次之,竞争力效应贡献较低。经二层分解后发现增长效应发挥了主要作用;同时中国出口的纺织品结构与RCEP市场进口需求相适应;竞争力效应的分解显示中国特定产品的竞争力仍有待提升。This paper uses UN Comtrade to conduct an in-depth study of the factors affecting China’s textile exports to other RCEP member countries through the Constant-Market-Share Model (CMS model). It also provides targeted policy recommendations and market strategies for governments and enterprises to achieve the growth and optimization of textile exports. This paper finds that the structural effect, competitiveness effect, and cross effect all promote China’s textile exports to RCEP countries, with the structural effect making the highest contribution, the cross effect the second highest, and the competitiveness effect making a lower contribution. After two-level decomposition, it is found that the growth effect plays a major role;at the same time, the textile structure of China’s exports is compatible with the import demand of the RCEP market;the decomposition of the competitiveness effect shows that the competitiveness of China’s specific products still needs to be improved.展开更多
基金supported by Heilongjiang Provincial Natural Science Foundation of China(Grant No.YQ2022C012)China Postdoctoral Science Foundation(Grant No.2022MD713728)+1 种基金Heilongjiang Provincial Postdoctoral Fund(Grant No.LBHZ21046)the Open Project of Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs,and National Key Research and Development Program of China(Grant No.2023YFD1201501).
文摘Ogura cytoplasmic male sterility(Ogura CMS)is extensively applied in hybrid seed production in cruciferous crops.However,the posttranscriptional molecular basis of Ogura CMS in cruciferous crops remains elusive.Here,a data-independent acquisition-based proteomic approach coupled with a parallel reaction monitoring-based targeted proteomic assay was used to analyze the proteome dynamics of Ogura CMS cabbage line RM and its maintainer line RF during floral bud development to obtain insights into the mechanism underlying Ogura CMS in cruciferous crops.A total of 9162 proteins corresponding to 61464 peptides were identified in RM and RF floral buds.The proteomic fluctuation of RM was weaker than that of RF.Differences in protein expression between RM and RF gradually enlarged with floral bud development.Fifteen continually up-regulated and eight continually down-regulated proteins were found in RM relative to RF throughout floral bud development.Differentially expressed proteins between RM and RF during floral bud development were implicated in the endoplasmic reticulum(ER)-associated protein processing pathway,in which most of them exhibited down-regulated expression in RM.These data suggest that ER-associated protein processing may be involved in pollen abortion in Ogura CMS cabbage by inhibiting the expression of critical factors.Our findings not only deepen the understanding of the molecular mechanisms of Ogura CMS in cruciferous crops but also provide better guidance for applying Ogura CMS in the hybrid breeding of cruciferous crops.
文摘本文利用联合国商品贸易数据库,通过恒定市场份额模型(CMS模型)深入研究中国对RCEP其他成员国纺织品出口的影响因素。并通过分析这些因素,为政府和企业提供有针对性的政策建议和市场战略,以实现纺织品出口的增长和优化。本文发现结构效应、竞争力效应、交叉效应都推动了我国对RCEP国家纺织品的出口,其中结构效应的贡献最高,交叉效应次之,竞争力效应贡献较低。经二层分解后发现增长效应发挥了主要作用;同时中国出口的纺织品结构与RCEP市场进口需求相适应;竞争力效应的分解显示中国特定产品的竞争力仍有待提升。This paper uses UN Comtrade to conduct an in-depth study of the factors affecting China’s textile exports to other RCEP member countries through the Constant-Market-Share Model (CMS model). It also provides targeted policy recommendations and market strategies for governments and enterprises to achieve the growth and optimization of textile exports. This paper finds that the structural effect, competitiveness effect, and cross effect all promote China’s textile exports to RCEP countries, with the structural effect making the highest contribution, the cross effect the second highest, and the competitiveness effect making a lower contribution. After two-level decomposition, it is found that the growth effect plays a major role;at the same time, the textile structure of China’s exports is compatible with the import demand of the RCEP market;the decomposition of the competitiveness effect shows that the competitiveness of China’s specific products still needs to be improved.
