SKG mouse,as a model of spontaneous rheumatoid arthritis (RA) bred recent years,is similar to the patients with RA.We analyzed the clonotypes of T cell infiltrating into joints of SKG mice in initial stage and late st...SKG mouse,as a model of spontaneous rheumatoid arthritis (RA) bred recent years,is similar to the patients with RA.We analyzed the clonotypes of T cell infiltrating into joints of SKG mice in initial stage and late stage of RA by using reverse transcriptase-polymerase chain reaction (RT-PCR) and subsequent single-strand conformation polymorphism (SSCP).The results indicated that the percentages of clonotypes TCR Vβ2 and Vβ8.2 of T cell clonotypes increased obviously to 72.3% and 60.2%,respectively.Mice number with identical TCR Vβ2 and Vβ8.2 clonotypes also clearly increased in late stage of disease to 100% and 75%,respectively. These results mean that T cells with TCR Vβ2 and Vβ8.2 clonotypes probably play an important role in RA progression of SKG mouse.Sequencing of the extracted DNA verified that common bands on SSCP gel were derived from the same T cell clones among samples from different joints.The results we obtained implied that RT-PCR/SSCP method was a sensitive and credible method for analyzing T cell clonotypes.When the T cells of SKG mouse were adoptively transferred to a nude mouse,it was verified that the T cells infiltrating into joints were related to morbid formation of RA.Cellular & Molecular Immunology.2004;1(4):300-303.展开更多
The diversity of antigen receptors and the specificity it underlies are the hallmarks of the cellular arm of the adaptive immune system. T and B lymphocytes are indeed truly unique in their ability to generate recepto...The diversity of antigen receptors and the specificity it underlies are the hallmarks of the cellular arm of the adaptive immune system. T and B lymphocytes are indeed truly unique in their ability to generate receptors capable of recognizing virtually any pathogen. It has been known for several decades that T lymphocytes recognize short peptides derived from degraded proteins presented by major histocompatibility complex （MHC） molecules at the cell surface. Interaction between peptide-MHC （pMHC） and the T cell receptor （TCR） is central to both thymic selection and peripheral antigen recognition. It is widely assumed that TCR diversity is required, or at least highly desirable, to provide sufficient immune coverage. However, a number of immune responses are associated with the selection of predictable, narrow, or skewed repertoires and public TCR chains. Here, we summarize the current knowledge on the formation of the TCR repertoire and its maintenance in health and disease. We also outline the various molecular mechanisms that govern the composition of the pre-selection, naive and antigen-specific TCR repertoires. Finally, we suggest that with the development of high-throughput sequencing, common TCR ＇signatures＇ raised against specific antigens could provide important diagnostic biomarkers and surrogate predictors of disease onset, progression and outcome.展开更多
文摘SKG mouse,as a model of spontaneous rheumatoid arthritis (RA) bred recent years,is similar to the patients with RA.We analyzed the clonotypes of T cell infiltrating into joints of SKG mice in initial stage and late stage of RA by using reverse transcriptase-polymerase chain reaction (RT-PCR) and subsequent single-strand conformation polymorphism (SSCP).The results indicated that the percentages of clonotypes TCR Vβ2 and Vβ8.2 of T cell clonotypes increased obviously to 72.3% and 60.2%,respectively.Mice number with identical TCR Vβ2 and Vβ8.2 clonotypes also clearly increased in late stage of disease to 100% and 75%,respectively. These results mean that T cells with TCR Vβ2 and Vβ8.2 clonotypes probably play an important role in RA progression of SKG mouse.Sequencing of the extracted DNA verified that common bands on SSCP gel were derived from the same T cell clones among samples from different joints.The results we obtained implied that RT-PCR/SSCP method was a sensitive and credible method for analyzing T cell clonotypes.When the T cells of SKG mouse were adoptively transferred to a nude mouse,it was verified that the T cells infiltrating into joints were related to morbid formation of RA.Cellular & Molecular Immunology.2004;1(4):300-303.
文摘The diversity of antigen receptors and the specificity it underlies are the hallmarks of the cellular arm of the adaptive immune system. T and B lymphocytes are indeed truly unique in their ability to generate receptors capable of recognizing virtually any pathogen. It has been known for several decades that T lymphocytes recognize short peptides derived from degraded proteins presented by major histocompatibility complex （MHC） molecules at the cell surface. Interaction between peptide-MHC （pMHC） and the T cell receptor （TCR） is central to both thymic selection and peripheral antigen recognition. It is widely assumed that TCR diversity is required, or at least highly desirable, to provide sufficient immune coverage. However, a number of immune responses are associated with the selection of predictable, narrow, or skewed repertoires and public TCR chains. Here, we summarize the current knowledge on the formation of the TCR repertoire and its maintenance in health and disease. We also outline the various molecular mechanisms that govern the composition of the pre-selection, naive and antigen-specific TCR repertoires. Finally, we suggest that with the development of high-throughput sequencing, common TCR ＇signatures＇ raised against specific antigens could provide important diagnostic biomarkers and surrogate predictors of disease onset, progression and outcome.
