The cleavage of DNA （pBR322） by [Cu（Dime-biim）2（H2O）]（ClO4）2 （Dime-biim=1, 1＇- dimethyl-2, 2＇-biimidazole） was investigated. The results showed that the complex could cleave DNA efficiently at pH=8.0 and 3...The cleavage of DNA （pBR322） by [Cu（Dime-biim）2（H2O）]（ClO4）2 （Dime-biim=1, 1＇- dimethyl-2, 2＇-biimidazole） was investigated. The results showed that the complex could cleave DNA efficiently at pH=8.0 and 37℃.展开更多
Copper (II) complex derived from 2, 2'-pyridil ligand has been, demonstrated to cleave efficiently DNA at micromolar concentrations at room temperature and pH 7.25.
Introduction Nucleases which catalyze the hydrolytic cleavage of the phosphodiester backbone of DNA and RN A play animportant role in the molecular genetics and genetic engineering.The development of reagents which hy...Introduction Nucleases which catalyze the hydrolytic cleavage of the phosphodiester backbone of DNA and RN A play animportant role in the molecular genetics and genetic engineering.The development of reagents which hydrolytically cleave nucleic acids under mild conditions has currently been attracting great interest in the field of artificial nucleases"",for example,the small metal complexes that promote the hydrolysis of DNA and RNA are useful not only in molecular biology and drug design but also in elucidating the role of metal ions in enzyme catalysis.展开更多
基金The authors acknowledge the support of the National Natural Science Foundation of China No.:2017 103 1)the Shanxi Provincial Natural Science Foundation for Youth(No.:20011007).
文摘The cleavage of DNA （pBR322） by [Cu（Dime-biim）2（H2O）]（ClO4）2 （Dime-biim=1, 1＇- dimethyl-2, 2＇-biimidazole） was investigated. The results showed that the complex could cleave DNA efficiently at pH=8.0 and 37℃.
基金Young Science Foundation of Shanxi Province (No.20001005).
文摘Copper (II) complex derived from 2, 2'-pyridil ligand has been, demonstrated to cleave efficiently DNA at micromolar concentrations at room temperature and pH 7.25.
文摘Introduction Nucleases which catalyze the hydrolytic cleavage of the phosphodiester backbone of DNA and RN A play animportant role in the molecular genetics and genetic engineering.The development of reagents which hydrolytically cleave nucleic acids under mild conditions has currently been attracting great interest in the field of artificial nucleases"",for example,the small metal complexes that promote the hydrolysis of DNA and RNA are useful not only in molecular biology and drug design but also in elucidating the role of metal ions in enzyme catalysis.
