Subject Code:C04With the support by the National Natural Science Foundation of China,a collaborative study by Chinese and American biologists led by Prof.Zhang Peng(张鹏)from Sun Yat-Sen University shows that nearly n...Subject Code:C04With the support by the National Natural Science Foundation of China,a collaborative study by Chinese and American biologists led by Prof.Zhang Peng(张鹏)from Sun Yat-Sen University shows that nearly nine out of 10species of frog today have descended from just three lineages that survived the展开更多
Background:Bush clearing of encroached rangelands is crucial for restoration of herbaceous cover and diversity.Regeneration after bush clearing depends largely on the soil seed bank(SSB)size and composition.To assess ...Background:Bush clearing of encroached rangelands is crucial for restoration of herbaceous cover and diversity.Regeneration after bush clearing depends largely on the soil seed bank(SSB)size and composition.To assess the potential of the SSB to facilitate post-clearing herbaceous restoration,we examined the SSB density,composition and diversity and similarity between SSB and aboveground vegetation(AGV).Methods:The study was conducted in semi-arid rangelands of Maseding and Kgomokgomo in North-West Province of South Africa.In each rangeland,all woody plants in three 1250 m^(2) plots were cut using saws and loppers,with the stumps treated with picloram.Paired observations were conducted in cleared and uncleared microsites.Soil samples were collected at three depths(0-10,10-20 and 20-30 cm)in each microsite in April(pretreatment),August,October and December 2016 and in April and August 2017.SSB was assessed using seedling emergence method,whereas pre-and post-clearing surveys of AGV were conducted in February 2016 and 2017,respectively.Results:Cleared microsites had significantly(p<0.01)higher seed bank densities(1872 and 693 seeds m^(-2) at Maseding and Kgomokgomo,respectively)relative to uncleared microsites in August 2017,with grasses accounting for higher densities than other plant functional groups in the upper 0-10 cm.Pioneer grasses(Aristida congesta subsp.barbicollis,Brachiaria eruciformis and Tragus berteronianus)and invasive forbs(Bidens pilosa and Schkuhria pinnata)dominated the SSB in cleared microsites at Maseding,whereas succulents(Portulaca spp.)and pioneer grasses(T.berteronianus and Urochloa mosambicensis)were abundant at Kgomokgomo in August 2017.SSB and AGV were dissimilar until December 2016;thereafter,similarity increased significantly(p<0.05)in cleared microsites(Sørensen’s coefficient=0.60 to 0.66 at Maseding and 0.43 to 0.52 at Kgomokgomo)compared to uncleared microsites.Species diversity was highest in August 2016 and April 2017 at Maseding,but it did not differ between two microsites(p>0.05).At Kgomokgomo,SSB was more diverse in cleared than uncleared microsites in April and August 2017.Conclusion:High seed bank densities in the upper soil layer in cleared microsites would promote passive restoration,but regeneration is likely to be initiated by early successional species.However,close monitoring and eradication of invasive forbs and succulents is necessary following bush clearing.The tendency of SSB resembling AGV over time in cleared microsites signifies that seed production from AGV is important for passive restoration following bush clearing.展开更多
The quest for high spatial resolution in molecular identification is critical across various domains,including physiology,pathology,and pharmaceutical research.Super-resolution microscopy has made strides by surpassin...The quest for high spatial resolution in molecular identification is critical across various domains,including physiology,pathology,and pharmaceutical research.Super-resolution microscopy has made strides by surpassing the Abbe diffraction limit,but it relies on sophisticated equipment and is limited by the sample size to handle.Expansion microscopy,an emerging technique,has broadened the scope of subdiffraction imaging.It chemically preserves tissues at a large scale and physically enlarges them 4−20 times linearly,enabling superresolution observation.This review begins by exploring the foundational concepts of tissue clearing and the latest methodologies in the field.It then delves into the core tenets of expansion microscopy,covering a range of protocols.The review spotlights advancements in enhancing resolution,improving labeling efficiency,and ensuring isotropic tissue expansion.Finally,the review offers insights into the prospective evolution of expansion microscopy.It emphasizes the potential role of machine learning in refining image quality and in the autonomous extraction of data,which could revolutionize the way we visualize and understand biological tissues.展开更多
Clear aligner treatment is a novel technique in current orthodontic practice.Distinct from traditional fixed orthodontic appliances,clear aligners have different material features and biomechanical characteristics and...Clear aligner treatment is a novel technique in current orthodontic practice.Distinct from traditional fixed orthodontic appliances,clear aligners have different material features and biomechanical characteristics and treatment efficiencies,presenting new clinical challenges.Therefore,a comprehensive and systematic description of the key clinical aspects of clear aligner treatment is essential to enhance treatment efficacy and facilitate the advancement and wide adoption of this new technique.This expert consensus discusses case selection and grading of treatment difficulty,principle of clear aligner therapy,clinical procedures and potential complications,which are crucial to the clinical success of clear aligner treatment.展开更多
Have you ever heard of a "refuge for stars"?Located at an average elevation of over 4,500 meters,Ngari Prefecture boasts world-class night-sky conditions due to minimal atmospheric pollution,abundant clear d...Have you ever heard of a "refuge for stars"?Located at an average elevation of over 4,500 meters,Ngari Prefecture boasts world-class night-sky conditions due to minimal atmospheric pollution,abundant clear days,high transparency,low humidity.展开更多
Types of paper Research Article A complete report on original research.A clear structure is required,including introduction,material and methods,results and discussion,conclusions generally.
Purpose–As an important part of the management of railway passenger transport,the rationality and effectiveness of the clearing method of railway passenger transport are directly related to the operating efficiency a...Purpose–As an important part of the management of railway passenger transport,the rationality and effectiveness of the clearing method of railway passenger transport are directly related to the operating efficiency and service quality of railway passenger transport enterprises.This paper aims to comprehensively and deeply discuss the evolution and development process of China’s railway passenger transport clearing method,analyze its characteristics and influences in each stage,identify the main factors affecting its evolution and development and then put forward thoughts on improving the future development of the clearing method.Design/methodology/approach–Through a detailed review of the railway passenger transport clearing methods from the planned economy period to the reform and opening up period and into the new century,the basis,mode,subject and object of clearing in different development stages are systematically compared.Findings–It comprehensively reveals the evolution of the clearing method,sorted out the characteristics and changes of the clearing method at each stage and the adaptability to the development of railway passenger transport at that time.The characteristics of the development of clearing measures for railway passenger transport in different stages and their far-reaching influence on railway passenger transport business are deeply analyzed.Originality/value–This paper summarized the factors influencing the development of China’s railway passenger transportation clearing approach evolution,including the simplified rules of clearing,enhanced the market adaptability,establishing and perfecting the incentive mechanism,strengthening the construction of informatization,etc.This paper puts forward the ways to improve the railway passenger transportation clearing future development thinking.展开更多
In late May,the city of Dunhuang,a key historical hub of Eastern and Western cultures in Northwest China's Gansu Province,basked in clear and refreshing weather under a brilliant blue sky.The rugged Mingsha Mounta...In late May,the city of Dunhuang,a key historical hub of Eastern and Western cultures in Northwest China's Gansu Province,basked in clear and refreshing weather under a brilliant blue sky.The rugged Mingsha Mountain,renowned for the musical sounds produced by wind-blown sands when the wind blows,stood in silent majesty.To the close north of the mountain's massive golden dunes stood the city's international convention centre,where some 400 vips from 60 countries host cultural exchanges and mutual learning.展开更多
Learning a foreign language like English needs patience and right methods.Grammar,which helps us form correct and clear sentences,is a basic part we can't ignore.We can review grammar rules through textbook exerci...Learning a foreign language like English needs patience and right methods.Grammar,which helps us form correct and clear sentences,is a basic part we can't ignore.We can review grammar rules through textbook exercises.To improve pronunciation,listening to native speakers'conversations on English podcasts and repeating after them works well.展开更多
Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment...Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment.Methods:Public databases were employed to identify Cedrenol,an active component of Bupleuri Radix,and its associated gene targets.Among these,the gene RORC(also known as RORγ)was selected for detailed analysis due to its high expression correlation.Expression levels of RORC across various cancers were assessed using the TCGA pan-cancer dataset.Further analyses,including differential expression,prognostic relevance,immune infiltration,single-gene,and functional enrichment analyses,were conducted using the TCGA ccRCC dataset.Additionally,potential drug sensitivities and molecular docking interactions with RORC were explored using the GSCALite and CellMiner databases.The effects of RORC on ccRCC were also validated through cellular experiments.Results:RORC exhibited elevated expression in clear cell renal carcinoma tissue in contrast to normal tissues,and lower RORC expression was related to better prognosis.Immune cell infiltration analysis suggested that RORC may influence the penetration of cells that inhibit immune responses,such as regulatory T cells,thereby affecting ccRCC progression.Furthermore,molecular docking studies revealed that several drugs,including Axitinib,Docetaxel,Methotrexate,and Temsirolimus,have a high affinity for RORC and exhibit strong molecular binding.In cellular experiment experiments confirmed that RORC knockdown led to reduced proliferation,metastasis,and ccRCC cell invasion.Conclusion:The study implies that RORC may be a potential gene target for Bupleuri Radix in the management of ccRCC.展开更多
BACKGROUND Metastasis of renal cell carcinoma(RCC)to the skeletal muscle and small bowel is an exceedingly rare occurrence.Both of these sites are unusual sites for RCC to metastasize to and to occur simultaneously is...BACKGROUND Metastasis of renal cell carcinoma(RCC)to the skeletal muscle and small bowel is an exceedingly rare occurrence.Both of these sites are unusual sites for RCC to metastasize to and to occur simultaneously is even less common.CASE SUMMARY A 58-year-old male with known history of RCC presented with a recurrence that was diagnosed through imaging and biopsies.Mucosa abnormalities of small bowel noted during endoscopy were biopsied as well as lesion in the psoas muscle that was noted.CONCLUSION This case report emphasizes that RCC can not only recur but can do so even decades later and present as metastatic foci at atypical sites.展开更多
Dear Editor,The mammalian brain exhibits cross-scale complexity in neuronal morphology and connectivity,the study of which demands high-resolution morphological reconstruction of individual neurons across the entire b...Dear Editor,The mammalian brain exhibits cross-scale complexity in neuronal morphology and connectivity,the study of which demands high-resolution morphological reconstruction of individual neurons across the entire brain[1-4].Current commonly used approaches for such mesoscale brain mapping include two main types of three-dimensional fluorescence microscopy:the block-face methods,and the lightsheet-based methods[5,6].In general,the high imaging speed and light efficiency of light-sheet microscopy make it a suitable tool for high-throughput volumetric imaging,especially when combined with tissue-clearing techniques.However,large brain samples pose major challenges to this approach.展开更多
Introduction Human papillomavirus(HPV)vaccination is a cornerstone of cervical cancer prevention,particularly in low-and middle-income countries(LMICs),where the burden of disease remains high~1.The World Health Organ...Introduction Human papillomavirus(HPV)vaccination is a cornerstone of cervical cancer prevention,particularly in low-and middle-income countries(LMICs),where the burden of disease remains high~1.The World Health Organization(WHO)HPV Vaccine Introduction Clearing House reported that 147 countries(of 194 reporting)had fully introduced the HPV vaccine into their national schedules as of 20242.After COVID-19 pandemic disruptions,global coverage is again increasing.展开更多
Background:Clear cell renal carcinoma(ccRCC),the leading histological subtype of RCC,lacks any targeted therapy options.Although some studies have shown that early growth response factor 1(EGR1)has a significant role ...Background:Clear cell renal carcinoma(ccRCC),the leading histological subtype of RCC,lacks any targeted therapy options.Although some studies have shown that early growth response factor 1(EGR1)has a significant role in cancer development and progression,its role and underlying mechanisms in ccRCC remain poorly understood.Methods:The Cancer Genome Atlas(TCGA)database was utilized to examine the expression of EGR1 in ccRCC.The expression of EGR1 in 55 ccRCC tissues was evaluated using immunohistochemistry.The link between EGR1 expression and clinicopathological variables was examined through an analysis.Gain-of-function assays were employed to investigate EGR1’s biological functions in ccRCC cells,involving proliferation,colony formation,invasion assays,and tumorigenesis in nude mice.In order to assess the protein expression of mitogen-activated protein kinase 15(MAPK15),E-cadherin,matrix metalloproteinase-9/-2(MMP-9 and MMP-2),Western blot technique was applied.Results:The results revealed a decrease in EGR1 expression in ccRCC tissues.This decrease was strongly linked to TNM stage,lymphatic metastasis,tumor size,histological grade,and unfavorable prognosis in ccRCC patients.It has been demonstrated that overexpressing EGR1 inhibits the growth of xenograft tumors in vivo and inhibits cell colony formation,motility,and invasion in vitro.Furthermore,EGR1 can prevent the development and movement of ccRCC cells by controlling the expression of MMP-2,MMP-9,E-cadherin,and MAPK15.Conclusions:The EGR1/MAPK15 axis may represent a promising target for drug development,with EGR1 serving as a possible target for ccRCC therapy.展开更多
Background:Clear cell renal cell carcinoma(ccRCC)is an aggressive malignancy associated with limited treatment options and poor prognosis.Emerging studies suggest that the actin-regulating protein actin-related protei...Background:Clear cell renal cell carcinoma(ccRCC)is an aggressive malignancy associated with limited treatment options and poor prognosis.Emerging studies suggest that the actin-regulating protein actin-related protein 2/3 complex subunit 1B(ARPC1B),a key regulatory protein within the actin cytoskeleton,could play a pivotal role in ccRCC progression.The current study aimed to uncover the biological functions of ARPC1B and the molecular mechanisms driving its effects in ccRCC.Methods:ARPC1B expression and prognostic implications were analyzed using data sourced from the Gene Expression Profiling Interactive Analysis(GEPIA)platform,immunohistochemical(IHC)staining on 150 tumor samples along with 30 corresponding normal tissues,and Western blotting(WB)analyses across multiple ccRCC-derived cell lines.Functional assays assessing cell proliferation,colony formation capability,migration,invasion,and in vivo tumorigenicity were conducted following either ARPC1B suppression or upregulation.Additionally,WB analysis was utilized to evaluate proteins linked to epithelial-to-mesenchymal transition(EMT)and the Wnt/β-catenin pathway.Results:The findings revealed a substantial elevation of ARPC1B in ccRCC tissues and cell lines,significantly associated with advanced TNM stages,higher Fuhrman grades,and reduced overall survival(OS)(p<0.001).Multivariate statistical analysis identified ARPC1B as a standalone prognostic factor.Silencing ARPC1B notably impaired ccRCC cellular activities,and tumorigenesis in animalmodels,whereas augmented ARPC1B expression enhanced these malignant phenotypes.Mechanistically,downregulation of ARPC1B suppressed Wnt/β-catenin signaling and disrupted EMT,indicated by reducedβ-catenin,c-Myc,cyclin D1,and ZEB-1 levels,and concurrently increased E-cadherin expression.Additionally,reactivation of theWnt/β-catenin pathway partly reversed the inhibitory effects of ARPC1B depletion on tumor growth and invasiveness.Conclusions:ARPC1B emerges as an essential oncogenic factor in ccRCC by stimulating EMT and activating the Wnt/β-catenin pathway,ultimately enhancing tumor aggressiveness and metastatic potential.Thus,targeting ARPC1B represents a promising therapeutic strategy,warranting further exploration in ccRCC management.展开更多
本文对求解不可压缩流体流动和传热问题提出了一种全隐算法。该算法被称为CLEAR(Coupled and LinkedEquations Algorithm Revised).该算法不同与SIMPLE系列算法之处在于,它用直接求解的压力改进速度,而不引入压力修正项。完全考虑了邻...本文对求解不可压缩流体流动和传热问题提出了一种全隐算法。该算法被称为CLEAR(Coupled and LinkedEquations Algorithm Revised).该算法不同与SIMPLE系列算法之处在于,它用直接求解的压力改进速度,而不引入压力修正项。完全考虑了邻点速度的影响,速度和压力的耦合得到很好的保证。因此在很大程度上加快了迭代的收敛速度,而且可以通过引入第二松弛因子,对迭代过程进行控制。本文详细阐述了CLEAR算法的数学原理和计算步骤,并讨论与SIMPLER算法的区别。在本文下篇中用五个算例对CLEAR算法和SIMPLER算法进行比较,证明了该算法的可行性。展开更多
文摘Subject Code:C04With the support by the National Natural Science Foundation of China,a collaborative study by Chinese and American biologists led by Prof.Zhang Peng(张鹏)from Sun Yat-Sen University shows that nearly nine out of 10species of frog today have descended from just three lineages that survived the
基金This research was supported by the Agricultural Research Council(MMN 3014)National Research Foundation(NRF)of South Africa.
文摘Background:Bush clearing of encroached rangelands is crucial for restoration of herbaceous cover and diversity.Regeneration after bush clearing depends largely on the soil seed bank(SSB)size and composition.To assess the potential of the SSB to facilitate post-clearing herbaceous restoration,we examined the SSB density,composition and diversity and similarity between SSB and aboveground vegetation(AGV).Methods:The study was conducted in semi-arid rangelands of Maseding and Kgomokgomo in North-West Province of South Africa.In each rangeland,all woody plants in three 1250 m^(2) plots were cut using saws and loppers,with the stumps treated with picloram.Paired observations were conducted in cleared and uncleared microsites.Soil samples were collected at three depths(0-10,10-20 and 20-30 cm)in each microsite in April(pretreatment),August,October and December 2016 and in April and August 2017.SSB was assessed using seedling emergence method,whereas pre-and post-clearing surveys of AGV were conducted in February 2016 and 2017,respectively.Results:Cleared microsites had significantly(p<0.01)higher seed bank densities(1872 and 693 seeds m^(-2) at Maseding and Kgomokgomo,respectively)relative to uncleared microsites in August 2017,with grasses accounting for higher densities than other plant functional groups in the upper 0-10 cm.Pioneer grasses(Aristida congesta subsp.barbicollis,Brachiaria eruciformis and Tragus berteronianus)and invasive forbs(Bidens pilosa and Schkuhria pinnata)dominated the SSB in cleared microsites at Maseding,whereas succulents(Portulaca spp.)and pioneer grasses(T.berteronianus and Urochloa mosambicensis)were abundant at Kgomokgomo in August 2017.SSB and AGV were dissimilar until December 2016;thereafter,similarity increased significantly(p<0.05)in cleared microsites(Sørensen’s coefficient=0.60 to 0.66 at Maseding and 0.43 to 0.52 at Kgomokgomo)compared to uncleared microsites.Species diversity was highest in August 2016 and April 2017 at Maseding,but it did not differ between two microsites(p>0.05).At Kgomokgomo,SSB was more diverse in cleared than uncleared microsites in April and August 2017.Conclusion:High seed bank densities in the upper soil layer in cleared microsites would promote passive restoration,but regeneration is likely to be initiated by early successional species.However,close monitoring and eradication of invasive forbs and succulents is necessary following bush clearing.The tendency of SSB resembling AGV over time in cleared microsites signifies that seed production from AGV is important for passive restoration following bush clearing.
基金the financial support from the Fundamental Research Funds for the Central Universities(Grant Nos.14380532,2024300419)Natural science foundation of Jiangsu province(Grant Nos.BK20220780,BE2022818).
文摘The quest for high spatial resolution in molecular identification is critical across various domains,including physiology,pathology,and pharmaceutical research.Super-resolution microscopy has made strides by surpassing the Abbe diffraction limit,but it relies on sophisticated equipment and is limited by the sample size to handle.Expansion microscopy,an emerging technique,has broadened the scope of subdiffraction imaging.It chemically preserves tissues at a large scale and physically enlarges them 4−20 times linearly,enabling superresolution observation.This review begins by exploring the foundational concepts of tissue clearing and the latest methodologies in the field.It then delves into the core tenets of expansion microscopy,covering a range of protocols.The review spotlights advancements in enhancing resolution,improving labeling efficiency,and ensuring isotropic tissue expansion.Finally,the review offers insights into the prospective evolution of expansion microscopy.It emphasizes the potential role of machine learning in refining image quality and in the autonomous extraction of data,which could revolutionize the way we visualize and understand biological tissues.
文摘Clear aligner treatment is a novel technique in current orthodontic practice.Distinct from traditional fixed orthodontic appliances,clear aligners have different material features and biomechanical characteristics and treatment efficiencies,presenting new clinical challenges.Therefore,a comprehensive and systematic description of the key clinical aspects of clear aligner treatment is essential to enhance treatment efficacy and facilitate the advancement and wide adoption of this new technique.This expert consensus discusses case selection and grading of treatment difficulty,principle of clear aligner therapy,clinical procedures and potential complications,which are crucial to the clinical success of clear aligner treatment.
文摘Have you ever heard of a "refuge for stars"?Located at an average elevation of over 4,500 meters,Ngari Prefecture boasts world-class night-sky conditions due to minimal atmospheric pollution,abundant clear days,high transparency,low humidity.
文摘Types of paper Research Article A complete report on original research.A clear structure is required,including introduction,material and methods,results and discussion,conclusions generally.
基金supported by the Research and Development Plan Project of China State Railway Group Co.,Ltd.(Project No.P2023X029).
文摘Purpose–As an important part of the management of railway passenger transport,the rationality and effectiveness of the clearing method of railway passenger transport are directly related to the operating efficiency and service quality of railway passenger transport enterprises.This paper aims to comprehensively and deeply discuss the evolution and development process of China’s railway passenger transport clearing method,analyze its characteristics and influences in each stage,identify the main factors affecting its evolution and development and then put forward thoughts on improving the future development of the clearing method.Design/methodology/approach–Through a detailed review of the railway passenger transport clearing methods from the planned economy period to the reform and opening up period and into the new century,the basis,mode,subject and object of clearing in different development stages are systematically compared.Findings–It comprehensively reveals the evolution of the clearing method,sorted out the characteristics and changes of the clearing method at each stage and the adaptability to the development of railway passenger transport at that time.The characteristics of the development of clearing measures for railway passenger transport in different stages and their far-reaching influence on railway passenger transport business are deeply analyzed.Originality/value–This paper summarized the factors influencing the development of China’s railway passenger transportation clearing approach evolution,including the simplified rules of clearing,enhanced the market adaptability,establishing and perfecting the incentive mechanism,strengthening the construction of informatization,etc.This paper puts forward the ways to improve the railway passenger transportation clearing future development thinking.
文摘In late May,the city of Dunhuang,a key historical hub of Eastern and Western cultures in Northwest China's Gansu Province,basked in clear and refreshing weather under a brilliant blue sky.The rugged Mingsha Mountain,renowned for the musical sounds produced by wind-blown sands when the wind blows,stood in silent majesty.To the close north of the mountain's massive golden dunes stood the city's international convention centre,where some 400 vips from 60 countries host cultural exchanges and mutual learning.
文摘Learning a foreign language like English needs patience and right methods.Grammar,which helps us form correct and clear sentences,is a basic part we can't ignore.We can review grammar rules through textbook exercises.To improve pronunciation,listening to native speakers'conversations on English podcasts and repeating after them works well.
基金supported by Hubei Provincial Department of Science and Technology,Innovation and Development Joint Fund(No.2022CFD145)Young Talents Project of Hubei Provincial Administration of Traditional Chinese Medicine(No.ZY2023Q002).
文摘Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment.Methods:Public databases were employed to identify Cedrenol,an active component of Bupleuri Radix,and its associated gene targets.Among these,the gene RORC(also known as RORγ)was selected for detailed analysis due to its high expression correlation.Expression levels of RORC across various cancers were assessed using the TCGA pan-cancer dataset.Further analyses,including differential expression,prognostic relevance,immune infiltration,single-gene,and functional enrichment analyses,were conducted using the TCGA ccRCC dataset.Additionally,potential drug sensitivities and molecular docking interactions with RORC were explored using the GSCALite and CellMiner databases.The effects of RORC on ccRCC were also validated through cellular experiments.Results:RORC exhibited elevated expression in clear cell renal carcinoma tissue in contrast to normal tissues,and lower RORC expression was related to better prognosis.Immune cell infiltration analysis suggested that RORC may influence the penetration of cells that inhibit immune responses,such as regulatory T cells,thereby affecting ccRCC progression.Furthermore,molecular docking studies revealed that several drugs,including Axitinib,Docetaxel,Methotrexate,and Temsirolimus,have a high affinity for RORC and exhibit strong molecular binding.In cellular experiment experiments confirmed that RORC knockdown led to reduced proliferation,metastasis,and ccRCC cell invasion.Conclusion:The study implies that RORC may be a potential gene target for Bupleuri Radix in the management of ccRCC.
文摘BACKGROUND Metastasis of renal cell carcinoma(RCC)to the skeletal muscle and small bowel is an exceedingly rare occurrence.Both of these sites are unusual sites for RCC to metastasize to and to occur simultaneously is even less common.CASE SUMMARY A 58-year-old male with known history of RCC presented with a recurrence that was diagnosed through imaging and biopsies.Mucosa abnormalities of small bowel noted during endoscopy were biopsied as well as lesion in the psoas muscle that was noted.CONCLUSION This case report emphasizes that RCC can not only recur but can do so even decades later and present as metastatic foci at atypical sites.
基金supported by the STI 2030-Major Project(2021ZD0204400,2022ZD0205203,2021ZD0200104,2022ZD0211900)the Shenzhen Science and Technology Program(RCYX20210706092100003,RCBS20221008093311027)+3 种基金the Shenzhen Medical Research Funds(A2303005)the Youth Innovation Promotion Association CAS(2022367)the National Natural Science Foundation of China(32100896)NSFC-Guangdong Joint Fund(U20A6005).
文摘Dear Editor,The mammalian brain exhibits cross-scale complexity in neuronal morphology and connectivity,the study of which demands high-resolution morphological reconstruction of individual neurons across the entire brain[1-4].Current commonly used approaches for such mesoscale brain mapping include two main types of three-dimensional fluorescence microscopy:the block-face methods,and the lightsheet-based methods[5,6].In general,the high imaging speed and light efficiency of light-sheet microscopy make it a suitable tool for high-throughput volumetric imaging,especially when combined with tissue-clearing techniques.However,large brain samples pose major challenges to this approach.
文摘Introduction Human papillomavirus(HPV)vaccination is a cornerstone of cervical cancer prevention,particularly in low-and middle-income countries(LMICs),where the burden of disease remains high~1.The World Health Organization(WHO)HPV Vaccine Introduction Clearing House reported that 147 countries(of 194 reporting)had fully introduced the HPV vaccine into their national schedules as of 20242.After COVID-19 pandemic disruptions,global coverage is again increasing.
基金supported by grants from the Shenzhen Basic Research Project(JCYJ20210324125803008)the Shenzhen Longhua District Science and Innovation Bureau Fund for Medical Institutions(2022143).
文摘Background:Clear cell renal carcinoma(ccRCC),the leading histological subtype of RCC,lacks any targeted therapy options.Although some studies have shown that early growth response factor 1(EGR1)has a significant role in cancer development and progression,its role and underlying mechanisms in ccRCC remain poorly understood.Methods:The Cancer Genome Atlas(TCGA)database was utilized to examine the expression of EGR1 in ccRCC.The expression of EGR1 in 55 ccRCC tissues was evaluated using immunohistochemistry.The link between EGR1 expression and clinicopathological variables was examined through an analysis.Gain-of-function assays were employed to investigate EGR1’s biological functions in ccRCC cells,involving proliferation,colony formation,invasion assays,and tumorigenesis in nude mice.In order to assess the protein expression of mitogen-activated protein kinase 15(MAPK15),E-cadherin,matrix metalloproteinase-9/-2(MMP-9 and MMP-2),Western blot technique was applied.Results:The results revealed a decrease in EGR1 expression in ccRCC tissues.This decrease was strongly linked to TNM stage,lymphatic metastasis,tumor size,histological grade,and unfavorable prognosis in ccRCC patients.It has been demonstrated that overexpressing EGR1 inhibits the growth of xenograft tumors in vivo and inhibits cell colony formation,motility,and invasion in vitro.Furthermore,EGR1 can prevent the development and movement of ccRCC cells by controlling the expression of MMP-2,MMP-9,E-cadherin,and MAPK15.Conclusions:The EGR1/MAPK15 axis may represent a promising target for drug development,with EGR1 serving as a possible target for ccRCC therapy.
文摘Background:Clear cell renal cell carcinoma(ccRCC)is an aggressive malignancy associated with limited treatment options and poor prognosis.Emerging studies suggest that the actin-regulating protein actin-related protein 2/3 complex subunit 1B(ARPC1B),a key regulatory protein within the actin cytoskeleton,could play a pivotal role in ccRCC progression.The current study aimed to uncover the biological functions of ARPC1B and the molecular mechanisms driving its effects in ccRCC.Methods:ARPC1B expression and prognostic implications were analyzed using data sourced from the Gene Expression Profiling Interactive Analysis(GEPIA)platform,immunohistochemical(IHC)staining on 150 tumor samples along with 30 corresponding normal tissues,and Western blotting(WB)analyses across multiple ccRCC-derived cell lines.Functional assays assessing cell proliferation,colony formation capability,migration,invasion,and in vivo tumorigenicity were conducted following either ARPC1B suppression or upregulation.Additionally,WB analysis was utilized to evaluate proteins linked to epithelial-to-mesenchymal transition(EMT)and the Wnt/β-catenin pathway.Results:The findings revealed a substantial elevation of ARPC1B in ccRCC tissues and cell lines,significantly associated with advanced TNM stages,higher Fuhrman grades,and reduced overall survival(OS)(p<0.001).Multivariate statistical analysis identified ARPC1B as a standalone prognostic factor.Silencing ARPC1B notably impaired ccRCC cellular activities,and tumorigenesis in animalmodels,whereas augmented ARPC1B expression enhanced these malignant phenotypes.Mechanistically,downregulation of ARPC1B suppressed Wnt/β-catenin signaling and disrupted EMT,indicated by reducedβ-catenin,c-Myc,cyclin D1,and ZEB-1 levels,and concurrently increased E-cadherin expression.Additionally,reactivation of theWnt/β-catenin pathway partly reversed the inhibitory effects of ARPC1B depletion on tumor growth and invasiveness.Conclusions:ARPC1B emerges as an essential oncogenic factor in ccRCC by stimulating EMT and activating the Wnt/β-catenin pathway,ultimately enhancing tumor aggressiveness and metastatic potential.Thus,targeting ARPC1B represents a promising therapeutic strategy,warranting further exploration in ccRCC management.
文摘本文对求解不可压缩流体流动和传热问题提出了一种全隐算法。该算法被称为CLEAR(Coupled and LinkedEquations Algorithm Revised).该算法不同与SIMPLE系列算法之处在于,它用直接求解的压力改进速度,而不引入压力修正项。完全考虑了邻点速度的影响,速度和压力的耦合得到很好的保证。因此在很大程度上加快了迭代的收敛速度,而且可以通过引入第二松弛因子,对迭代过程进行控制。本文详细阐述了CLEAR算法的数学原理和计算步骤,并讨论与SIMPLER算法的区别。在本文下篇中用五个算例对CLEAR算法和SIMPLER算法进行比较,证明了该算法的可行性。
