Joint clearances in antenna pointing mechanisms lead to uncertainty in function deviation. Current studies mainly focus on radial clearance of revolute joints, while axial clearance has rarely been taken into consider...Joint clearances in antenna pointing mechanisms lead to uncertainty in function deviation. Current studies mainly focus on radial clearance of revolute joints, while axial clearance has rarely been taken into consideration. In fact, own?ing to errors from machining and assembly, thermal deformation and so forth, practically, axial clearance is inevitable in the joint. In this study, an error equivalent model(EEM) of revolute joints is proposed with considering both radial and axial clearances. Compared to the planar model of revolute joints only considering radial clearance, the journal motion inside the bearing is more abundant and matches the reality better in the EEM. The model is also extended for analyzing the error distribution of a spatial dual?axis("X–Y" type) antenna pointing mechanism of Spot?beam antennas which especially demand a high pointing accuracy. Three case studies are performed which illustrates the internal relation between radial clearance and axial clearance. It is found that when the axial clearance is big enough, the physical journal can freely realize both translational motion and rotational motion. While if the axial clearance is limited, the motion of the physical journal will be restricted. Analysis results indicate that the consideration of both radial and axial clearances in the revolute joint describes the journal motion inside the bearing more precise. To further validate the proposed model, a model of the EEM is designed and fabricated. Some suggestions on the design of revolute joints are also provided.展开更多
There are clearances in mechanism because of manufacture and assembly error,which reduces operation life and working accuracy of mechanism and has a great impact on dynamical responses.At the moment,research in this a...There are clearances in mechanism because of manufacture and assembly error,which reduces operation life and working accuracy of mechanism and has a great impact on dynamical responses.At the moment,research in this area mainly focuses on single degree⁃of⁃freedom mechanism considering one clearance,while research of multi⁃DOF mechanism considering multi⁃clearance is less.With the purpose of studying the dynamical characteristics of complex multi⁃DOF mechanism with multi⁃clearances,a dynamic model was developed.The dynamic responses of 2⁃DOF mechanism with two clearances under different positions,values,and numbers of clearance were analyzed.The displacement,velocity,acceleration,collision force,and the axis trajectory at clearance were then given.In addition,there is a limited amount of literature on chaotic phenomena,which mainly focuses on the chaotic phenomena of end⁃effector of mechanism.But in this paper,the non⁃linear characteristics were analyzed by chaotic phenomenon of clearance joint,then chaotic phenomenon was identified by Poincarémappings and phase diagrams.Bifurcation diagrams were given.The results will offer a reliable technical support for the study of dynamical responses of planar mechanisms and the analysis of chaotic phenomena.展开更多
The inner and outer oil film dynamic characteristic coefficients of floating ring bearings（FRBs） change due to the manufacturing tolerance of the floating ring, journal and intermediate, which leads to high-speed tu...The inner and outer oil film dynamic characteristic coefficients of floating ring bearings（FRBs） change due to the manufacturing tolerance of the floating ring, journal and intermediate, which leads to high-speed turbocharger＇s vibration too large and even causes nonlinear vibration accident. However, the investigation of floating ring bearing manufacturing tolerance clearance on the rotordynamic characteristics is less at present. In order to study the influence law of inner and outer clearance on turbocharger vibration, the rotor dynamic motion equations of turbocharger supported in FRBs are derived by analyzing the size relations between floating ring, journal and intermediate for the inner and outer oil film clearances, the time transient response analysis for combination of FRBs clearance are developed. A realistic turbocharger is taken as a research object, the FE model of the turbocharger with FRBs is modeled. Under the conditions of four kinds of limit state bearing clearances for inner and outer oil film, the nonlinear transient analyses are performed based on the established FE dynamic models of the nonlinear rotor-FRBs system applied incentive combinations of gravity and unbalance force, respectively. From the waterfall, the simulation results show that the speed for the appearance of fractional frequency is not identical and the amplitude magnitude is different under the four kinds of bearing manufacturing tolerance limit clearances, and fractional frequency does not appear in the turbocharger and the amplitude is minimum under the ODMin/IDMax bearing manufacturing tolerance clearances. The turbocharger vibration is reduced by controlling the manufacturing tolerance clearance combinations of FRBs, which is helpful for the dynamic design and production-manufacturing of high-speed turbocharger.展开更多
To investigate the effect of tip clearance size on cavitation characteristics in a turbopump inducer,a series of experiments have been conducted in a newly developed visualization test facility using room temperature ...To investigate the effect of tip clearance size on cavitation characteristics in a turbopump inducer,a series of experiments have been conducted in a newly developed visualization test facility using room temperature water as working fluid.The pressure fluctuations near the tip region were collected,and the cavity structures under various conditions were documented by a high-speed camera.It is found that large tip clearance distinctly reduces both the non-cavitation and cavitation performance.Three cavitation instabilities,super-synchronous rotating cavitation,synchronous rotating cavitation and cavitation surge have been carefully identified through combination of cross-correlation analysis of pressure signals and visualization results.Large tip clearance displays a remarkable stabilization effect on pressure fluctuation,cavitation surge totally disappears,and the range of occurrence of synchronous rotating cavitation becomes smaller for the large tip clearance,whereas super-synchronous rotating cavitation only occurs in the large tip clearance.The cavitation areas are smaller at large tip clearance,while the flow channels are more seriously choked when cavitation occurs heavily in comparison with those at small tip clearance,which may be responsible for the worse cavitation performance.展开更多
This paper uses the topology theory to analyze the surface flowspectrums of straight, positively curved and negatively curvedcascades with relative tip clearances of 0.023 and 0.036, findsapparent differences of topol...This paper uses the topology theory to analyze the surface flowspectrums of straight, positively curved and negatively curvedcascades with relative tip clearances of 0.023 and 0.036, findsapparent differences of topology and vortex structures in the bladetip and the suction side wall corner of single type of cascade withthis two clearances, and studies the mechanism of the differenceformation as well as their effects o the energy loss.展开更多
A new non-linear transverse-torsional coupled model was proposed for 2K-H planetary gear train, and gear's geometric eccentricity error, comprehensive transmission error, time-varying meshing stiffness, sun-planet...A new non-linear transverse-torsional coupled model was proposed for 2K-H planetary gear train, and gear's geometric eccentricity error, comprehensive transmission error, time-varying meshing stiffness, sun-planet and planet-ring gear pair's backlashes and sun gear's bearing clearance were taken into consideration. The solution of differential governing equation of motion was solved by applying variable step-size Runge-Kutta numerical integration method. The system motion state was investigated systematically and qualitatively, and exhibited diverse characteristics of bifurcation and chaos as well as non-linear behavior under different bifurcation parameters including meshing frequency, sun-planet backlash, planet-ring backlash and sun gear's bearing clearance. Analysis results show that the increasing damping could suppress the region of chaotic motion and improve the system's stability significantly. The route of crisis to chaotic motion was observed under the bifurcation parameter of meshing frequency. However, the routes of period doubling and crisis to chaos were identified under the bifurcation parameter of sun-planet backlash; besides, several different types of routes to chaos were observed and coexisted under the bifurcation parameter of planet-ring backlash including period doubling, Hopf bifurcation, 3T-periodic channel and crisis. Additionally, planet-ring backlash generated a strong coupling effect to system's non-linear behavior while the sun gear's bearing clearance produced weak coupling effect. Finally, quasi-periodic motion could be found under all above–mentioned bifurcation parameters and closely associated with the 3T-periodic motion.展开更多
It has been well known that nozzle end-clearances in a Variable Nozzle Turbine（VNT）are unfavorable for aerodynamic performance, especially at small openings, and efforts to further decrease size of the clearances ar...It has been well known that nozzle end-clearances in a Variable Nozzle Turbine（VNT）are unfavorable for aerodynamic performance, especially at small openings, and efforts to further decrease size of the clearances are very hard due to thermal expansion. In this paper, both the different sizes of nozzle end-clearances and the various ratios of their distribution at the hub and shroud sides were modelled and investigated by performing 3D Computational Fluid Dynamics（CFD） and Finite Element Analysis（FEA） simulations with a code of transferring the aerodynamic pressure from the CFD results to the FEA calculations. It was found that increasing the size of the nozzle end-clearances divided equally at the hub and shroud sides deteriorates turbine efficiency and turbine wheel reliability, yet increases turbine flow capacity. And, when the total nozzle endclearances remain the same, varying nozzle end-clearances＇ distribution at the hub and shroud sides not only shifts operation point of a VNT turbine, but also affects the turbine wheel vibration stress.Compared with nozzle hub clearance, the shroud clearance is more sensitive to both aerodynamic performance and reliability of a VNT turbine. Consequently, a possibility is put forward to improve VNT turbine efficiency meanwhile decrease vibration stress by optimizing nozzle end-clearances＇ distribution.展开更多
This paper is concerned with the determination of the normal force-displacement （NFD） relation for the contact problem of cylindrical joints with clearance. A simple formulation for this contact problem is developed...This paper is concerned with the determination of the normal force-displacement （NFD） relation for the contact problem of cylindrical joints with clearance. A simple formulation for this contact problem is developed by modeling the pin as a rigid wedge and the elastic plate as a simple Winkler elastic foundation. The numerical results show that the normal displacement relation based on Hertz theory is only valid for the case of large clearance with a small normal load, and the NFD relation based on Persson theory is only effective in the case of very small clearance. The proposed approximate model in this paper gives better results than Hertz theory and Persson theory in a large range of clearances as seen from the comparison with the results of FEM.展开更多
A new nonlinear transverse-torsional coupled model with backlash and bearing clearance was proposed for planetary gear set. Meanwhile, sun gear and planet's eccentricity errors, static transmission error, and time...A new nonlinear transverse-torsional coupled model with backlash and bearing clearance was proposed for planetary gear set. Meanwhile, sun gear and planet's eccentricity errors, static transmission error, and time-varying meshing stiffness were taken into consideration. The differential governing equations of motion were solved by employing variable step-size Rung-Kutta numerical integration method. The behavior of dynamic load sharing characteristics affected by the system parameters including input rate, sun gear's supporting stiffness and eccentricity error, planet's eccentricity error, sun gear's bearing clearance, backlashes of sun-planet and planet-ring meshes were investigated qualitatively and systematically. Some theoretical results are summarized at last which extend the current understanding of the dynamic load sharing behavior of planet gear train, enrich the related literature and provide references for the design of planetary gear train.展开更多
This article reports on track-monitoring and analyzing machine clearances during wood forwarding across seasons and weather, using ultrasonic distance sensors in combination with time-stamped GPS xy locations, at 10 s...This article reports on track-monitoring and analyzing machine clearances during wood forwarding across seasons and weather, using ultrasonic distance sensors in combination with time-stamped GPS xy locations, at 10 sec intervals. The resulting data, obtained from 54 harvesting blocks, were analyzed by machine type (two wood forwarders and one grapple skidder), stand type (softwood plantation versus natural hardwood stands), month, slope, cartographic depth-to-water (DTW) classes, number of passes along track, and machine speed. For the most part, clearances were highly variable, due to passing over stumps, rocks, harvest slash, brushmats, ruts, and snow cover when present. This variability was on average greater for the lighter-weight wood forwarders than for the heavier-weight skidder, with the former mostly moving along equally spaced lines on brushmats, while the paths of the latter spread away from central wood-landing sites. In terms of trends, machines moved 1) more slowly on wet ground, 2) faster during returning than forwarding, and 3) fastest along wood-landing roads, as to be expected. Low clearances were most notable during winter on snow-covered ground, and on non-frozen shallow DTW and wet multiple-pass ground. During dry weather conditions, clearances also increased from low-pass tracks to multi-pass tracks due to repeat soil compaction of broadened tracks. These results are presented block-by-block and by machine type. Each block-based clearance frequency pattern was quantified through regression analysis and using a gamma probability distribution function.展开更多
This paper presents a numerical investigation of effects of axial non-uniform tip clearances on the aerodynamic performance of a transonic axial compressor rotor （NASA Rotor 37）. The three-dimensional steady flow fi...This paper presents a numerical investigation of effects of axial non-uniform tip clearances on the aerodynamic performance of a transonic axial compressor rotor （NASA Rotor 37）. The three-dimensional steady flow field within the rotor passage was simulated with the datum tip clearance of 0.356 mm at the design wheel speed of 17188.7 rpm. The simulation results are well consistent with the measurement results, which verified the numeri- cal method. Then the three-dimensional steady flow field within the rotor passage was simulated respectively with different axial non-uniform tip clearances. The calculation results showed that optimal axial non-uniform tip clearances could improve the compressor performance, while the efficiency and the pressure ratio of the com- pressor were increased. The flow mechanism is that the axial non-uniform tip clearance can weaken the tip leak- age vortex, blow down low-energy fluids in boundary layers and reduce both flow blockage and tip loss.展开更多
The excessive buildup of neurotoxicα-synuclein plays a pivotal role in the pathogenesis of Parkinson's disease,highlighting the urgent need for innovative therapeutic strategies to promoteα-synuclein clearance,p...The excessive buildup of neurotoxicα-synuclein plays a pivotal role in the pathogenesis of Parkinson's disease,highlighting the urgent need for innovative therapeutic strategies to promoteα-synuclein clearance,particularly given the current lack of disease-modifying treatments.The glymphatic system,a recently identified perivascular fluid transport network,is crucial for clearing neurotoxic proteins.This review aims to synthesize current knowledge on the role of the glymphatic system inα-synuclein clearance and its implications for the pathology of Parkinson's disease while emphasizing potential therapeutic strategies and areas for future research.The review begins with an overview of the glymphatic system and details its anatomical structure and physiological functions that facilitate cerebrospinal fluid circulation and waste clearance.It summarizes emerging evidence from neuroimaging and experimental studies that highlight the close correlation between the glymphatic system and clinical symptom severity in patients with Parkinson's disease,as well as the effect of glymphatic dysfunction onα-synuclein accumulation in Parkinson's disease models.Subsequently,the review summarizes the mechanisms of glymphatic system impairment in Parkinson's disease,including sleep disturbances,aquaporin-4 impairment,and mitochondrial dysfunction,all of which diminish glymphatic system efficiency.This creates a vicious cycle that exacerbatesα-synuclein accumulation and worsens Parkinson's disease.The therapeutic perspectives section outlines strategies for enhancing glymphatic activity,such as improving sleep quality and pharmacologically targeting aquaporin-4 or its subcellular localization.Promising interventions include deep brain stimulation,melatonin supplementation,γ-aminobutyric acid modulation,and non-invasive methods(such as exercise and bright-light therapy),multisensoryγstimulation,and ultrasound therapy.Moreover,identifying neuroimaging biomarkers to assess glymphatic flow as an indicator ofα-synuclein burden could refine Parkinson's disease diagnosis and track disease progression.In conclusion,the review highlights the critical role of the glymphatic system inα-synuclein clearance and its potential as a therapeutic target in Parkinson's disease.It advocates for further research to elucidate the specific mechanisms by which the glymphatic system clears misfoldedα-synuclein and the development of imaging biomarkers to monitor glymphatic activity in patients with Parkinson's disease.Findings from this review suggest that enhancing glymphatic clearance is a promising strategy for reducingα-synuclein deposits and mitigating the progression of Parkinson's disease.展开更多
Circumferentially non-uniform tip clearances induced by rotor eccentricity significantly affect the overall performance of axial compressors,particularly the stability margin.Currently,Computational Fluid Dynamics(CFD...Circumferentially non-uniform tip clearances induced by rotor eccentricity significantly affect the overall performance of axial compressors,particularly the stability margin.Currently,Computational Fluid Dynamics(CFD)plays a crucial role in the aerodynamic analysis of eccentric compressors.However,conventional full-annulus Unsteady Reynolds-Averaged Navier-Stokes(URANS)simulations are prohibitively expensive for routine design and analysis purposes.To address this issue,the paper presents a novel Fourier-based method,called the Time-Space Collocation(TSC)method,for efficient simulations of eccentric compressors.This method coherently treats temporal and spatial harmonics,making it well-suited to tackle the rotor eccentricity problem,as the perturbation waves induced by eccentricity are time-periodic with respect to the rotor and space-periodic with respect to the stator.Three numerical cases,including NASA Rotor 67,original Stage 67,and Stage 67 with a reduced rotor-stator axial gap,were conducted to verify the effectiveness of the TSC method.The results indicate that,for the rotor eccentricity levels studied in this paper,the influence of weak rotor-stator interactions can be disregarded in the original Stage 67.In this situation,applying three harmonics can accurately capture both the performance variations and the non-uniformly distributed flowfields of eccentric compressors,while achieving a reduction in run time by two orders of magnitude compared to full-annulus URANS simulations.However,in Stage 67 with a reduced rotor-stator axial gap,the results that include rotor-stator interactions align much more closely with the URANS results.Nevertheless,the TSC simulations can still achieve speed-ups of several dozen times.Overall,the TSC method shows promising potential for application within the engineering community.展开更多
Mitochondrial dysfunction has emerged as a critical factor in the etiology of various neurodevelopmental disorders, including autism spectrum disorders, attention-deficit/hyperactivity disorder, and Rett syndrome. Alt...Mitochondrial dysfunction has emerged as a critical factor in the etiology of various neurodevelopmental disorders, including autism spectrum disorders, attention-deficit/hyperactivity disorder, and Rett syndrome. Although these conditions differ in clinical presentation, they share fundamental pathological features that may stem from abnormal mitochondrial dynamics and impaired autophagic clearance, which contribute to redox imbalance and oxidative stress in neurons. This review aimed to elucidate the relationship between mitochondrial dynamics dysfunction and neurodevelopmental disorders. Mitochondria are highly dynamic organelles that undergo continuous fusion and fission to meet the substantial energy demands of neural cells. Dysregulation of these processes, as observed in certain neurodevelopmental disorders, causes accumulation of damaged mitochondria, exacerbating oxidative damage and impairing neuronal function. The phosphatase and tensin homolog-induced putative kinase 1/E3 ubiquitin-protein ligase pathway is crucial for mitophagy, the process of selectively removing malfunctioning mitochondria. Mutations in genes encoding mitochondrial fusion proteins have been identified in autism spectrum disorders, linking disruptions in the fusion-fission equilibrium to neurodevelopmental impairments. Additionally, animal models of Rett syndrome have shown pronounced defects in mitophagy, reinforcing the notion that mitochondrial quality control is indispensable for neuronal health. Clinical studies have highlighted the importance of mitochondrial disturbances in neurodevelopmental disorders. In autism spectrum disorders, elevated oxidative stress markers and mitochondrial DNA deletions indicate compromised mitochondrial function. Attention-deficit/hyperactivity disorder has also been associated with cognitive deficits linked to mitochondrial dysfunction and oxidative stress. Moreover, induced pluripotent stem cell models derived from patients with Rett syndrome have shown impaired mitochondrial dynamics and heightened vulnerability to oxidative injury, suggesting the role of defective mitochondrial homeostasis in these disorders. From a translational standpoint, multiple therapeutic approaches targeting mitochondrial pathways show promise. Interventions aimed at preserving normal fusion-fission cycles or enhancing mitophagy can reduce oxidative damage by limiting the accumulation of defective mitochondria. Pharmacological modulation of mitochondrial permeability and upregulation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, an essential regulator of mitochondrial biogenesis, may also ameliorate cellular energy deficits. Identifying early biomarkers of mitochondrial impairment is crucial for precision medicine, since it can help clinicians tailor interventions to individual patient profiles and improve prognoses. Furthermore, integrating mitochondria-focused strategies with established therapies, such as antioxidants or behavioral interventions, may enhance treatment efficacy and yield better clinical outcomes. Leveraging these pathways could open avenues for regenerative strategies, given the influence of mitochondria on neuronal repair and plasticity. In conclusion, this review indicates mitochondrial homeostasis as a unifying therapeutic axis within neurodevelopmental pathophysiology. Disruptions in mitochondrial dynamics and autophagic clearance converge on oxidative stress, and researchers should prioritize validating these interventions in clinical settings to advance precision medicine and enhance outcomes for individuals affected by neurodevelopmental disorders.展开更多
Alzheimer s disease is a devastating neurodegenerative disorder affecting millions worldwide,with current treatments offering only limited benefits.Central to emerging research is the role of autophagy and endolysosom...Alzheimer s disease is a devastating neurodegenerative disorder affecting millions worldwide,with current treatments offering only limited benefits.Central to emerging research is the role of autophagy and endolysosomal pathways,which are essential for clearing misfolded proteins and damaged organelles.Bridging integrator 1(BIN1),traditionally recognized for its role in membrane remodeling and endocytosis,has recently emerged as a top genetic risk factor for Alzheimer's disease,linking cellular clearance mechanisms to the development of toxic amyloid-beta plaques and tau tangles.In this review,we provide an accessible overview of how disruptions in autophagy and endolysosomal trafficking contribute to the neurodegeneration process in Alzheimer's disease,positioning BIN1 as a central mediator within this complex network.Recent advances have shown that alte rations in BIN1 expression and isoform distribution are associated with increased tau pathology and changes in amyloid-beta processing.Moreover,BIN1 appears to also influence synaptic transmission,neuroinflammation,and overall cellular homeostasis.The integration of recent findings not only deepens our understanding of Alzheimer s disease pathology but also opens new avenues for the development of targeted treatments.This timely perspective underscores the potential of modulating BIN1 activity to enhance cellular clearance mechanisms and offers hope for more effective inte rventions for Alzheimer's disease.展开更多
The dedicated freight channel at the Youyiguan Border Gate in south China’s Guangxi Zhuang Autonomous Region buzzes with activity as trucks loaded with export goods move steadily through customs.Thanks to a system up...The dedicated freight channel at the Youyiguan Border Gate in south China’s Guangxi Zhuang Autonomous Region buzzes with activity as trucks loaded with export goods move steadily through customs.Thanks to a system upgraded at the start of 2026,clearance personnel at this China-Vietnam crossing simply place an order online,and the entire process is handled almost instantly via an intelligent platform.Adding to the efficiency,the border gate also features dedicated lanes that support driverless container trucks.展开更多
Activation of spinal cord neural stem cells(NSCs)and subsequent neurogenesis holds a promising alternative for spinal cord injury(SCI)repair.Our previous study demonstrated that complement C3a,derived from reactive as...Activation of spinal cord neural stem cells(NSCs)and subsequent neurogenesis holds a promising alternative for spinal cord injury(SCI)repair.Our previous study demonstrated that complement C3a,derived from reactive astrocytes,inhibits NSC proliferation by suppressing protein aggregate clearance through the deubiquitinating enzyme ubiquitin carboxy-terminal hydrolase L1(UCHL1)-proteasome system post-SCI.However,the potential molecular mechanism by which C3a modulates NSC activation via this pathway remains unclear.Here,we revealed that C3a/C3a receptor(C3aR)signaling activated NF-κB p65,which in turn inhibited Nrf2 activity and UCHL1 expression,resulting in diminished proteasome activity and the accumulation of protein aggregates,and ultimately impaired NSC activation.Both knockdown of NF-κB p65 and Nrf2 upregulation restored UCHL1 expression and proteasome activity in vitro,promoting NSC activation by enhancing protein aggregate clearance.Mechanistically,we found that NF-κB p65 regulated Nrf2 activity through a dual mechanism:(1)promoting Keap1-dependent ubiquitination and proteasome degradation of Nrf2;(2)inhibiting protein kinase C-mediated Nrf2 phosphorylation and nuclear translocation.Using the dual-luciferase reporter assay and chromatin immunoprecipitation(ChIP)analysis,we further identified UCHL1 as a direct transcriptional target of Nrf2.Importantly,in vivo experiments using SCI mice confirmed that either C3aR blockade,NF-κB p65 knockdown,or Nrf2 overexpression could rescue SCI-induced UCHL1 downregulation.Together,this study uncovers the C3a-NF-κB p65-Nrf2-UCHL1-proteasome axis as a critical regulator of NSC activation after SCI.This may provide novel molecular targets and intervention strategies for SCI repair.展开更多
A comprehensive method to predict wear in planar mechanical systems with clearance joints is presented and discussed in this paper.This method consists of a system dynamic analysis and a joint wear prediction.As the s...A comprehensive method to predict wear in planar mechanical systems with clearance joints is presented and discussed in this paper.This method consists of a system dynamic analysis and a joint wear prediction.As the size and shape of the clearance are dictated by wear and evolve with the dynamic response of the system,the contact between the journal and bearing could be conformal or non-conformal,which makes the contact conditions in clearance joints quite complicated.Therefore a modified contact force model is employed to evaluate the joint reaction force in this study.As the nonlinear stiffness coefficient is related to the physical and geometrical properties of contact bodies and varies with the deformation,this contact force model is applicable to different contact conditions between the journal and bearing.Furthermore,based on the Archard’s wear model,the amount of wear can be quantified in the joint.And the geometry is updated to reflect the evolving contact boundary.Then,the wear process and the contact force model are integrated into the motion equations of the system to perform coupled iterative analyses between system dynamic response and joint wear prediction.In addition,a slider-crank mechanism is simulated as an example to demonstrate efficiency of the proposed method and to carry out a parametric study on mechanical systems considering joint wear.The influence of clearance size and driving power are discussed and compared respectively.The index of concordance is introduced to quantify contributions of contact pressure and sliding distance to wear rate under different types of journal motion.This study could help to predict joint wear in mechanical systems with clearances and optimize mechanisms in design.展开更多
In order to investigate the characteristics of a vertical axial flow pump under various clearances of flare tube, the bell-shaped inlet and box culvert outlet channels with flare tube are studied numerically and exper...In order to investigate the characteristics of a vertical axial flow pump under various clearances of flare tube, the bell-shaped inlet and box culvert outlet channels with flare tube are studied numerically and experimentally. Then, the cases of inlet and outlet channels with the least hydraulic loss are selected to form an integral pump system, for which both numerical simulation and experimental investigation are carried out. The numerical results agree well with the experimental data. It is shown that the clearances of the interfaces between different components of the pump system have a significant impact on the internal flow structure, turbulent entropy and hydraulic performance of the inlet and outlet channels. For the cases of normalized bottom clearance less than 0.5 and normalized top clearance larger than 0.4, the internal flow and hydraulic performance indexes of inlet and outlet channels are relatively poor. There also exists a critical clearance in either inlet or outlet channel at which the hydraulic loss reaches the maximum. The results serve as an important reference for the design as well as safe and efficient operation of the vertical axial flow pump system.展开更多
This paper presents investigations into the influences of bearing clearances on the diagnostic features of monitoring rolling-bearings. A nonlinear dynamic model of a deep groove ball bearing with five degrees of free...This paper presents investigations into the influences of bearing clearances on the diagnostic features of monitoring rolling-bearings. A nonlinear dynamic model of a deep groove ball bearing with five degrees of freedom is developed for numerical analysis under increased radial clearances which are due to not only the scenarios of bearing grades but also gradual wear with bearing service lifetime. The model incorporates local defects and clearance increments in order to gain the insight into the bearing dynamics under different fault cases along with clearance changes. Numerical results show that the vibrations at fault characteristic frequencies exhibit clear inconsistency with common understandings for different cases of increased clearances. This study highlights that it has to take into account the clearance effect, especially for the inner race fault, in order to avoid the under-estimate of fault sizes which may be indicated by the feature amplitude reduction.展开更多
基金Supported by National Natural Science Foundation of China(Grant Nos.51635002(Key Program),51605011,51275015)
文摘Joint clearances in antenna pointing mechanisms lead to uncertainty in function deviation. Current studies mainly focus on radial clearance of revolute joints, while axial clearance has rarely been taken into consideration. In fact, own?ing to errors from machining and assembly, thermal deformation and so forth, practically, axial clearance is inevitable in the joint. In this study, an error equivalent model(EEM) of revolute joints is proposed with considering both radial and axial clearances. Compared to the planar model of revolute joints only considering radial clearance, the journal motion inside the bearing is more abundant and matches the reality better in the EEM. The model is also extended for analyzing the error distribution of a spatial dual?axis("X–Y" type) antenna pointing mechanism of Spot?beam antennas which especially demand a high pointing accuracy. Three case studies are performed which illustrates the internal relation between radial clearance and axial clearance. It is found that when the axial clearance is big enough, the physical journal can freely realize both translational motion and rotational motion. While if the axial clearance is limited, the motion of the physical journal will be restricted. Analysis results indicate that the consideration of both radial and axial clearances in the revolute joint describes the journal motion inside the bearing more precise. To further validate the proposed model, a model of the EEM is designed and fabricated. Some suggestions on the design of revolute joints are also provided.
基金Sponsored by the Shandong Key Research and Development Public Welfare Program(Grant No.2019GGX104011)the Natural Science Foundation of Shandong Province(Grant No.ZR2017MEE066).
文摘There are clearances in mechanism because of manufacture and assembly error,which reduces operation life and working accuracy of mechanism and has a great impact on dynamical responses.At the moment,research in this area mainly focuses on single degree⁃of⁃freedom mechanism considering one clearance,while research of multi⁃DOF mechanism considering multi⁃clearance is less.With the purpose of studying the dynamical characteristics of complex multi⁃DOF mechanism with multi⁃clearances,a dynamic model was developed.The dynamic responses of 2⁃DOF mechanism with two clearances under different positions,values,and numbers of clearance were analyzed.The displacement,velocity,acceleration,collision force,and the axis trajectory at clearance were then given.In addition,there is a limited amount of literature on chaotic phenomena,which mainly focuses on the chaotic phenomena of end⁃effector of mechanism.But in this paper,the non⁃linear characteristics were analyzed by chaotic phenomenon of clearance joint,then chaotic phenomenon was identified by Poincarémappings and phase diagrams.Bifurcation diagrams were given.The results will offer a reliable technical support for the study of dynamical responses of planar mechanisms and the analysis of chaotic phenomena.
基金Supported by National Natural Science Foundation of China(Grant Nos.51205121,51375162)Scientific Research Foundation of Hunan Provincial Education Department of China(Grant No.13A023)Postgraduate Innovation Foundation of Hunan University of Science and Technology,China(Grant No.S140020)
文摘The inner and outer oil film dynamic characteristic coefficients of floating ring bearings（FRBs） change due to the manufacturing tolerance of the floating ring, journal and intermediate, which leads to high-speed turbocharger＇s vibration too large and even causes nonlinear vibration accident. However, the investigation of floating ring bearing manufacturing tolerance clearance on the rotordynamic characteristics is less at present. In order to study the influence law of inner and outer clearance on turbocharger vibration, the rotor dynamic motion equations of turbocharger supported in FRBs are derived by analyzing the size relations between floating ring, journal and intermediate for the inner and outer oil film clearances, the time transient response analysis for combination of FRBs clearance are developed. A realistic turbocharger is taken as a research object, the FE model of the turbocharger with FRBs is modeled. Under the conditions of four kinds of limit state bearing clearances for inner and outer oil film, the nonlinear transient analyses are performed based on the established FE dynamic models of the nonlinear rotor-FRBs system applied incentive combinations of gravity and unbalance force, respectively. From the waterfall, the simulation results show that the speed for the appearance of fractional frequency is not identical and the amplitude magnitude is different under the four kinds of bearing manufacturing tolerance limit clearances, and fractional frequency does not appear in the turbocharger and the amplitude is minimum under the ODMin/IDMax bearing manufacturing tolerance clearances. The turbocharger vibration is reduced by controlling the manufacturing tolerance clearance combinations of FRBs, which is helpful for the dynamic design and production-manufacturing of high-speed turbocharger.
基金supported by the National Basic Research Program of China(No.613321)。
文摘To investigate the effect of tip clearance size on cavitation characteristics in a turbopump inducer,a series of experiments have been conducted in a newly developed visualization test facility using room temperature water as working fluid.The pressure fluctuations near the tip region were collected,and the cavity structures under various conditions were documented by a high-speed camera.It is found that large tip clearance distinctly reduces both the non-cavitation and cavitation performance.Three cavitation instabilities,super-synchronous rotating cavitation,synchronous rotating cavitation and cavitation surge have been carefully identified through combination of cross-correlation analysis of pressure signals and visualization results.Large tip clearance displays a remarkable stabilization effect on pressure fluctuation,cavitation surge totally disappears,and the range of occurrence of synchronous rotating cavitation becomes smaller for the large tip clearance,whereas super-synchronous rotating cavitation only occurs in the large tip clearance.The cavitation areas are smaller at large tip clearance,while the flow channels are more seriously choked when cavitation occurs heavily in comparison with those at small tip clearance,which may be responsible for the worse cavitation performance.
文摘This paper uses the topology theory to analyze the surface flowspectrums of straight, positively curved and negatively curvedcascades with relative tip clearances of 0.023 and 0.036, findsapparent differences of topology and vortex structures in the bladetip and the suction side wall corner of single type of cascade withthis two clearances, and studies the mechanism of the differenceformation as well as their effects o the energy loss.
基金Projects(51375226,51305196,51475226) supported by the National Natural Science Foundation of ChinaProjects(NZ2013303,NZ2014201) supported by the Fundamental Research Funds for the Central Universities,China
文摘A new non-linear transverse-torsional coupled model was proposed for 2K-H planetary gear train, and gear's geometric eccentricity error, comprehensive transmission error, time-varying meshing stiffness, sun-planet and planet-ring gear pair's backlashes and sun gear's bearing clearance were taken into consideration. The solution of differential governing equation of motion was solved by applying variable step-size Runge-Kutta numerical integration method. The system motion state was investigated systematically and qualitatively, and exhibited diverse characteristics of bifurcation and chaos as well as non-linear behavior under different bifurcation parameters including meshing frequency, sun-planet backlash, planet-ring backlash and sun gear's bearing clearance. Analysis results show that the increasing damping could suppress the region of chaotic motion and improve the system's stability significantly. The route of crisis to chaotic motion was observed under the bifurcation parameter of meshing frequency. However, the routes of period doubling and crisis to chaos were identified under the bifurcation parameter of sun-planet backlash; besides, several different types of routes to chaos were observed and coexisted under the bifurcation parameter of planet-ring backlash including period doubling, Hopf bifurcation, 3T-periodic channel and crisis. Additionally, planet-ring backlash generated a strong coupling effect to system's non-linear behavior while the sun gear's bearing clearance produced weak coupling effect. Finally, quasi-periodic motion could be found under all above–mentioned bifurcation parameters and closely associated with the 3T-periodic motion.
基金co-supported by the Natural Science Foundation of Hebei Province of China(No.E2017402135)the Program of Science and Technology Research and Development of Handan of China(No.1621212047-2)
文摘It has been well known that nozzle end-clearances in a Variable Nozzle Turbine（VNT）are unfavorable for aerodynamic performance, especially at small openings, and efforts to further decrease size of the clearances are very hard due to thermal expansion. In this paper, both the different sizes of nozzle end-clearances and the various ratios of their distribution at the hub and shroud sides were modelled and investigated by performing 3D Computational Fluid Dynamics（CFD） and Finite Element Analysis（FEA） simulations with a code of transferring the aerodynamic pressure from the CFD results to the FEA calculations. It was found that increasing the size of the nozzle end-clearances divided equally at the hub and shroud sides deteriorates turbine efficiency and turbine wheel reliability, yet increases turbine flow capacity. And, when the total nozzle endclearances remain the same, varying nozzle end-clearances＇ distribution at the hub and shroud sides not only shifts operation point of a VNT turbine, but also affects the turbine wheel vibration stress.Compared with nozzle hub clearance, the shroud clearance is more sensitive to both aerodynamic performance and reliability of a VNT turbine. Consequently, a possibility is put forward to improve VNT turbine efficiency meanwhile decrease vibration stress by optimizing nozzle end-clearances＇ distribution.
基金The project supported by the National Natural Science Foundation of China(10272002:60334030)
文摘This paper is concerned with the determination of the normal force-displacement （NFD） relation for the contact problem of cylindrical joints with clearance. A simple formulation for this contact problem is developed by modeling the pin as a rigid wedge and the elastic plate as a simple Winkler elastic foundation. The numerical results show that the normal displacement relation based on Hertz theory is only valid for the case of large clearance with a small normal load, and the NFD relation based on Persson theory is only effective in the case of very small clearance. The proposed approximate model in this paper gives better results than Hertz theory and Persson theory in a large range of clearances as seen from the comparison with the results of FEM.
基金Project(51105194)supported by the National Natural Science Foundation of ChinaProject(20113218110017)supported by the Doctoral Program Foundation of Institutions of Higher Education of China+2 种基金Project supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions,ChinaProject(CXZZ11_0199)supported by the Funding of Jiangsu Innovation Program for Graduate Education,ChinaProjects(NZ2013303,NZ2014201)supported by the Fundamental Research Funds for the Central Universities,China
文摘A new nonlinear transverse-torsional coupled model with backlash and bearing clearance was proposed for planetary gear set. Meanwhile, sun gear and planet's eccentricity errors, static transmission error, and time-varying meshing stiffness were taken into consideration. The differential governing equations of motion were solved by employing variable step-size Rung-Kutta numerical integration method. The behavior of dynamic load sharing characteristics affected by the system parameters including input rate, sun gear's supporting stiffness and eccentricity error, planet's eccentricity error, sun gear's bearing clearance, backlashes of sun-planet and planet-ring meshes were investigated qualitatively and systematically. Some theoretical results are summarized at last which extend the current understanding of the dynamic load sharing behavior of planet gear train, enrich the related literature and provide references for the design of planetary gear train.
文摘This article reports on track-monitoring and analyzing machine clearances during wood forwarding across seasons and weather, using ultrasonic distance sensors in combination with time-stamped GPS xy locations, at 10 sec intervals. The resulting data, obtained from 54 harvesting blocks, were analyzed by machine type (two wood forwarders and one grapple skidder), stand type (softwood plantation versus natural hardwood stands), month, slope, cartographic depth-to-water (DTW) classes, number of passes along track, and machine speed. For the most part, clearances were highly variable, due to passing over stumps, rocks, harvest slash, brushmats, ruts, and snow cover when present. This variability was on average greater for the lighter-weight wood forwarders than for the heavier-weight skidder, with the former mostly moving along equally spaced lines on brushmats, while the paths of the latter spread away from central wood-landing sites. In terms of trends, machines moved 1) more slowly on wet ground, 2) faster during returning than forwarding, and 3) fastest along wood-landing roads, as to be expected. Low clearances were most notable during winter on snow-covered ground, and on non-frozen shallow DTW and wet multiple-pass ground. During dry weather conditions, clearances also increased from low-pass tracks to multi-pass tracks due to repeat soil compaction of broadened tracks. These results are presented block-by-block and by machine type. Each block-based clearance frequency pattern was quantified through regression analysis and using a gamma probability distribution function.
基金the National Natural Science Foundation of China,Grant No.50776004Aeronautics Foundation of China,Grant No.04C51030the 111 Project,No,B07009
文摘This paper presents a numerical investigation of effects of axial non-uniform tip clearances on the aerodynamic performance of a transonic axial compressor rotor （NASA Rotor 37）. The three-dimensional steady flow field within the rotor passage was simulated with the datum tip clearance of 0.356 mm at the design wheel speed of 17188.7 rpm. The simulation results are well consistent with the measurement results, which verified the numeri- cal method. Then the three-dimensional steady flow field within the rotor passage was simulated respectively with different axial non-uniform tip clearances. The calculation results showed that optimal axial non-uniform tip clearances could improve the compressor performance, while the efficiency and the pressure ratio of the com- pressor were increased. The flow mechanism is that the axial non-uniform tip clearance can weaken the tip leak- age vortex, blow down low-energy fluids in boundary layers and reduce both flow blockage and tip loss.
基金supported by the National Natural Science Foundation of China,No.81971031(to ZL)the National Key Research and Development Program of China,No.2022YFE0210100(to JFC)+7 种基金the National Natural Science Foundation of China(Original Exploration Project),No.82151308(to JFC)the National Natural Science Foundation of China(Research Fund for International Senior Scientists),No.82150710558(to JFC)Science&Technology Initiative STI2030-Major Projects,No.2021ZD0203400(to JFC)Key Research and Development Program of Zhejiang Province,No.2023C03079(to JFC)Scientific Research Starting Foundation of Oujiang Laboratory(Zhejiang Laboratory for Regenerative Medicine,Vision and Brain Health),No.OJQDSP2022007(to JFC)Project of State Key Laboratory of Ophthalmology,Optometry and Visual Science,Wenzhou Medical University,No.J01-20190101(to JFC)Scientific Research Starting Foundation of Wenzhou Medical University,No.QTJ12003(to JFC)Department of Science and Technology of Zhejiang Province,No.2023ZY1011(to JFC)。
文摘The excessive buildup of neurotoxicα-synuclein plays a pivotal role in the pathogenesis of Parkinson's disease,highlighting the urgent need for innovative therapeutic strategies to promoteα-synuclein clearance,particularly given the current lack of disease-modifying treatments.The glymphatic system,a recently identified perivascular fluid transport network,is crucial for clearing neurotoxic proteins.This review aims to synthesize current knowledge on the role of the glymphatic system inα-synuclein clearance and its implications for the pathology of Parkinson's disease while emphasizing potential therapeutic strategies and areas for future research.The review begins with an overview of the glymphatic system and details its anatomical structure and physiological functions that facilitate cerebrospinal fluid circulation and waste clearance.It summarizes emerging evidence from neuroimaging and experimental studies that highlight the close correlation between the glymphatic system and clinical symptom severity in patients with Parkinson's disease,as well as the effect of glymphatic dysfunction onα-synuclein accumulation in Parkinson's disease models.Subsequently,the review summarizes the mechanisms of glymphatic system impairment in Parkinson's disease,including sleep disturbances,aquaporin-4 impairment,and mitochondrial dysfunction,all of which diminish glymphatic system efficiency.This creates a vicious cycle that exacerbatesα-synuclein accumulation and worsens Parkinson's disease.The therapeutic perspectives section outlines strategies for enhancing glymphatic activity,such as improving sleep quality and pharmacologically targeting aquaporin-4 or its subcellular localization.Promising interventions include deep brain stimulation,melatonin supplementation,γ-aminobutyric acid modulation,and non-invasive methods(such as exercise and bright-light therapy),multisensoryγstimulation,and ultrasound therapy.Moreover,identifying neuroimaging biomarkers to assess glymphatic flow as an indicator ofα-synuclein burden could refine Parkinson's disease diagnosis and track disease progression.In conclusion,the review highlights the critical role of the glymphatic system inα-synuclein clearance and its potential as a therapeutic target in Parkinson's disease.It advocates for further research to elucidate the specific mechanisms by which the glymphatic system clears misfoldedα-synuclein and the development of imaging biomarkers to monitor glymphatic activity in patients with Parkinson's disease.Findings from this review suggest that enhancing glymphatic clearance is a promising strategy for reducingα-synuclein deposits and mitigating the progression of Parkinson's disease.
文摘Circumferentially non-uniform tip clearances induced by rotor eccentricity significantly affect the overall performance of axial compressors,particularly the stability margin.Currently,Computational Fluid Dynamics(CFD)plays a crucial role in the aerodynamic analysis of eccentric compressors.However,conventional full-annulus Unsteady Reynolds-Averaged Navier-Stokes(URANS)simulations are prohibitively expensive for routine design and analysis purposes.To address this issue,the paper presents a novel Fourier-based method,called the Time-Space Collocation(TSC)method,for efficient simulations of eccentric compressors.This method coherently treats temporal and spatial harmonics,making it well-suited to tackle the rotor eccentricity problem,as the perturbation waves induced by eccentricity are time-periodic with respect to the rotor and space-periodic with respect to the stator.Three numerical cases,including NASA Rotor 67,original Stage 67,and Stage 67 with a reduced rotor-stator axial gap,were conducted to verify the effectiveness of the TSC method.The results indicate that,for the rotor eccentricity levels studied in this paper,the influence of weak rotor-stator interactions can be disregarded in the original Stage 67.In this situation,applying three harmonics can accurately capture both the performance variations and the non-uniformly distributed flowfields of eccentric compressors,while achieving a reduction in run time by two orders of magnitude compared to full-annulus URANS simulations.However,in Stage 67 with a reduced rotor-stator axial gap,the results that include rotor-stator interactions align much more closely with the URANS results.Nevertheless,the TSC simulations can still achieve speed-ups of several dozen times.Overall,the TSC method shows promising potential for application within the engineering community.
文摘Mitochondrial dysfunction has emerged as a critical factor in the etiology of various neurodevelopmental disorders, including autism spectrum disorders, attention-deficit/hyperactivity disorder, and Rett syndrome. Although these conditions differ in clinical presentation, they share fundamental pathological features that may stem from abnormal mitochondrial dynamics and impaired autophagic clearance, which contribute to redox imbalance and oxidative stress in neurons. This review aimed to elucidate the relationship between mitochondrial dynamics dysfunction and neurodevelopmental disorders. Mitochondria are highly dynamic organelles that undergo continuous fusion and fission to meet the substantial energy demands of neural cells. Dysregulation of these processes, as observed in certain neurodevelopmental disorders, causes accumulation of damaged mitochondria, exacerbating oxidative damage and impairing neuronal function. The phosphatase and tensin homolog-induced putative kinase 1/E3 ubiquitin-protein ligase pathway is crucial for mitophagy, the process of selectively removing malfunctioning mitochondria. Mutations in genes encoding mitochondrial fusion proteins have been identified in autism spectrum disorders, linking disruptions in the fusion-fission equilibrium to neurodevelopmental impairments. Additionally, animal models of Rett syndrome have shown pronounced defects in mitophagy, reinforcing the notion that mitochondrial quality control is indispensable for neuronal health. Clinical studies have highlighted the importance of mitochondrial disturbances in neurodevelopmental disorders. In autism spectrum disorders, elevated oxidative stress markers and mitochondrial DNA deletions indicate compromised mitochondrial function. Attention-deficit/hyperactivity disorder has also been associated with cognitive deficits linked to mitochondrial dysfunction and oxidative stress. Moreover, induced pluripotent stem cell models derived from patients with Rett syndrome have shown impaired mitochondrial dynamics and heightened vulnerability to oxidative injury, suggesting the role of defective mitochondrial homeostasis in these disorders. From a translational standpoint, multiple therapeutic approaches targeting mitochondrial pathways show promise. Interventions aimed at preserving normal fusion-fission cycles or enhancing mitophagy can reduce oxidative damage by limiting the accumulation of defective mitochondria. Pharmacological modulation of mitochondrial permeability and upregulation of peroxisome proliferator-activated receptor gamma coactivator 1-alpha, an essential regulator of mitochondrial biogenesis, may also ameliorate cellular energy deficits. Identifying early biomarkers of mitochondrial impairment is crucial for precision medicine, since it can help clinicians tailor interventions to individual patient profiles and improve prognoses. Furthermore, integrating mitochondria-focused strategies with established therapies, such as antioxidants or behavioral interventions, may enhance treatment efficacy and yield better clinical outcomes. Leveraging these pathways could open avenues for regenerative strategies, given the influence of mitochondria on neuronal repair and plasticity. In conclusion, this review indicates mitochondrial homeostasis as a unifying therapeutic axis within neurodevelopmental pathophysiology. Disruptions in mitochondrial dynamics and autophagic clearance converge on oxidative stress, and researchers should prioritize validating these interventions in clinical settings to advance precision medicine and enhance outcomes for individuals affected by neurodevelopmental disorders.
基金National Institutes of Health,No.R01AG083943Alzheimer's Association Strategic Fund No.ABA-22-965518(to YWAH)。
文摘Alzheimer s disease is a devastating neurodegenerative disorder affecting millions worldwide,with current treatments offering only limited benefits.Central to emerging research is the role of autophagy and endolysosomal pathways,which are essential for clearing misfolded proteins and damaged organelles.Bridging integrator 1(BIN1),traditionally recognized for its role in membrane remodeling and endocytosis,has recently emerged as a top genetic risk factor for Alzheimer's disease,linking cellular clearance mechanisms to the development of toxic amyloid-beta plaques and tau tangles.In this review,we provide an accessible overview of how disruptions in autophagy and endolysosomal trafficking contribute to the neurodegeneration process in Alzheimer's disease,positioning BIN1 as a central mediator within this complex network.Recent advances have shown that alte rations in BIN1 expression and isoform distribution are associated with increased tau pathology and changes in amyloid-beta processing.Moreover,BIN1 appears to also influence synaptic transmission,neuroinflammation,and overall cellular homeostasis.The integration of recent findings not only deepens our understanding of Alzheimer s disease pathology but also opens new avenues for the development of targeted treatments.This timely perspective underscores the potential of modulating BIN1 activity to enhance cellular clearance mechanisms and offers hope for more effective inte rventions for Alzheimer's disease.
文摘The dedicated freight channel at the Youyiguan Border Gate in south China’s Guangxi Zhuang Autonomous Region buzzes with activity as trucks loaded with export goods move steadily through customs.Thanks to a system upgraded at the start of 2026,clearance personnel at this China-Vietnam crossing simply place an order online,and the entire process is handled almost instantly via an intelligent platform.Adding to the efficiency,the border gate also features dedicated lanes that support driverless container trucks.
基金supported by the National Natural Science Foundation of China(82071362 and 82270669)Key Project of the Regional Joint Fund of Guangdong Province(2023B1515120077)+3 种基金Basic Research Program of Shenzhen Science and Technology Innovation Commission(JCYJ20210324123001003 and JCYJ20220530144801003)Shenzhen Key Laboratory of Bone Tissue Repair and Translational Research(ZDSYS20230626091402006)the Innovation and Entrepreneurship Training Program for College Students,Sun Yat-sen University(20242150)the Leading Innovation and Entrepreneurship Team Program of Zhejiang Province,China(2023R01005).
文摘Activation of spinal cord neural stem cells(NSCs)and subsequent neurogenesis holds a promising alternative for spinal cord injury(SCI)repair.Our previous study demonstrated that complement C3a,derived from reactive astrocytes,inhibits NSC proliferation by suppressing protein aggregate clearance through the deubiquitinating enzyme ubiquitin carboxy-terminal hydrolase L1(UCHL1)-proteasome system post-SCI.However,the potential molecular mechanism by which C3a modulates NSC activation via this pathway remains unclear.Here,we revealed that C3a/C3a receptor(C3aR)signaling activated NF-κB p65,which in turn inhibited Nrf2 activity and UCHL1 expression,resulting in diminished proteasome activity and the accumulation of protein aggregates,and ultimately impaired NSC activation.Both knockdown of NF-κB p65 and Nrf2 upregulation restored UCHL1 expression and proteasome activity in vitro,promoting NSC activation by enhancing protein aggregate clearance.Mechanistically,we found that NF-κB p65 regulated Nrf2 activity through a dual mechanism:(1)promoting Keap1-dependent ubiquitination and proteasome degradation of Nrf2;(2)inhibiting protein kinase C-mediated Nrf2 phosphorylation and nuclear translocation.Using the dual-luciferase reporter assay and chromatin immunoprecipitation(ChIP)analysis,we further identified UCHL1 as a direct transcriptional target of Nrf2.Importantly,in vivo experiments using SCI mice confirmed that either C3aR blockade,NF-κB p65 knockdown,or Nrf2 overexpression could rescue SCI-induced UCHL1 downregulation.Together,this study uncovers the C3a-NF-κB p65-Nrf2-UCHL1-proteasome axis as a critical regulator of NSC activation after SCI.This may provide novel molecular targets and intervention strategies for SCI repair.
基金supported by the National Natural Science Foundation of China(Grant No.11272171)Specialized Research Fund for the Doctoral Program of Higher Education(Grant No.20120002110070)
文摘A comprehensive method to predict wear in planar mechanical systems with clearance joints is presented and discussed in this paper.This method consists of a system dynamic analysis and a joint wear prediction.As the size and shape of the clearance are dictated by wear and evolve with the dynamic response of the system,the contact between the journal and bearing could be conformal or non-conformal,which makes the contact conditions in clearance joints quite complicated.Therefore a modified contact force model is employed to evaluate the joint reaction force in this study.As the nonlinear stiffness coefficient is related to the physical and geometrical properties of contact bodies and varies with the deformation,this contact force model is applicable to different contact conditions between the journal and bearing.Furthermore,based on the Archard’s wear model,the amount of wear can be quantified in the joint.And the geometry is updated to reflect the evolving contact boundary.Then,the wear process and the contact force model are integrated into the motion equations of the system to perform coupled iterative analyses between system dynamic response and joint wear prediction.In addition,a slider-crank mechanism is simulated as an example to demonstrate efficiency of the proposed method and to carry out a parametric study on mechanical systems considering joint wear.The influence of clearance size and driving power are discussed and compared respectively.The index of concordance is introduced to quantify contributions of contact pressure and sliding distance to wear rate under different types of journal motion.This study could help to predict joint wear in mechanical systems with clearances and optimize mechanisms in design.
基金supported by the National Natural Science Foundation of China(Grant Nos.52079057,52106043)the China Postdoctoral Science Foundation(Grant No.2022M711376).
文摘In order to investigate the characteristics of a vertical axial flow pump under various clearances of flare tube, the bell-shaped inlet and box culvert outlet channels with flare tube are studied numerically and experimentally. Then, the cases of inlet and outlet channels with the least hydraulic loss are selected to form an integral pump system, for which both numerical simulation and experimental investigation are carried out. The numerical results agree well with the experimental data. It is shown that the clearances of the interfaces between different components of the pump system have a significant impact on the internal flow structure, turbulent entropy and hydraulic performance of the inlet and outlet channels. For the cases of normalized bottom clearance less than 0.5 and normalized top clearance larger than 0.4, the internal flow and hydraulic performance indexes of inlet and outlet channels are relatively poor. There also exists a critical clearance in either inlet or outlet channel at which the hydraulic loss reaches the maximum. The results serve as an important reference for the design as well as safe and efficient operation of the vertical axial flow pump system.
文摘This paper presents investigations into the influences of bearing clearances on the diagnostic features of monitoring rolling-bearings. A nonlinear dynamic model of a deep groove ball bearing with five degrees of freedom is developed for numerical analysis under increased radial clearances which are due to not only the scenarios of bearing grades but also gradual wear with bearing service lifetime. The model incorporates local defects and clearance increments in order to gain the insight into the bearing dynamics under different fault cases along with clearance changes. Numerical results show that the vibrations at fault characteristic frequencies exhibit clear inconsistency with common understandings for different cases of increased clearances. This study highlights that it has to take into account the clearance effect, especially for the inner race fault, in order to avoid the under-estimate of fault sizes which may be indicated by the feature amplitude reduction.
