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CCDC40基因新发突变导致男性不育临床分析(附1例家系报告)
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作者 陈亮 王玲 +6 位作者 杨志芳 王国霖 吴德宇 杨宇卓 陈菲 徐阳 薛晴 《中国性科学》 2025年第4期1-5,共5页
目的探讨CCDC40基因新发位点突变导致的男性不育的临床特点。方法通过精液分析、电镜超微形态学分析、基因点突变检测等技术进行分析。结果精液分析结果提示精子活动力差、精子尾部发育不全,电镜超微形态学分析显示头部及尾部畸形、精... 目的探讨CCDC40基因新发位点突变导致的男性不育的临床特点。方法通过精液分析、电镜超微形态学分析、基因点突变检测等技术进行分析。结果精液分析结果提示精子活动力差、精子尾部发育不全,电镜超微形态学分析显示头部及尾部畸形、精核发育不良、轴丝微管数量减少/缺失、排列紊乱等。全外显子测序发现CCDC40基因c.3009_3040dup致病性纯合移码变异。结论CCDC40基因c.3009_3040dup纯合移码突变改变了蛋白序列,导致超微结构缺陷及精子尾部畸形;该突变为首次被鉴定,丰富了基因突变谱,为临床诊疗提供了借鉴。 展开更多
关键词 ccdc40 基因突变 男性不育
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CCDC97影响肝癌的免疫微环境以及生物学功能 被引量:1
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作者 莫灵玲 吴新悦 +1 位作者 彭小花 陈闯 《细胞与分子免疫学杂志》 北大核心 2025年第1期23-30,共8页
目的探讨CCDC97与肝细胞癌(HCC)的临床及免疫价值。方法从癌症基因组图谱(TCGA)和国际癌症基因组联盟(ICGC)数据库中获取HCC患者的临床和RNA测序数据。通过生物信息学和数据库分析,研究CCDC97在HCC中的作用,并利用体外实验探讨其功能。... 目的探讨CCDC97与肝细胞癌(HCC)的临床及免疫价值。方法从癌症基因组图谱(TCGA)和国际癌症基因组联盟(ICGC)数据库中获取HCC患者的临床和RNA测序数据。通过生物信息学和数据库分析,研究CCDC97在HCC中的作用,并利用体外实验探讨其功能。结果HCC患者和肝癌细胞中CCDC97的表达水平高,并与病理特征、预后等密切相关。CCDC97被确认是一个新的预后因素。CCDC97与剪接体通路相关,该通路在肿瘤中高度活跃,可能促进癌变。CCDC97在多种免疫细胞中高表达,与免疫微环境有关。此外,敲低CCDC97在体外实验中抑制了细胞的侵袭、迁移和增殖。结论CCDC97在HCC进展以及免疫微环境中发挥重要作用,可能成为HCC预后和治疗的新靶点。 展开更多
关键词 肝细胞癌(HCC) ccdc97 免疫微环境 剪接体 免疫浸润
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Urban tree species classification based on multispectral airborne LiDAR 被引量:1
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作者 HU Pei-Lun CHEN Yu-Wei +3 位作者 Mohammad Imangholiloo Markus Holopainen WANG Yi-Cheng Juha Hyyppä 《红外与毫米波学报》 北大核心 2025年第2期211-216,共6页
Urban tree species provide various essential ecosystem services in cities,such as regulating urban temperatures,reducing noise,capturing carbon,and mitigating the urban heat island effect.The quality of these services... Urban tree species provide various essential ecosystem services in cities,such as regulating urban temperatures,reducing noise,capturing carbon,and mitigating the urban heat island effect.The quality of these services is influenced by species diversity,tree health,and the distribution and the composition of trees.Traditionally,data on urban trees has been collected through field surveys and manual interpretation of remote sensing images.In this study,we evaluated the effectiveness of multispectral airborne laser scanning(ALS)data in classifying 24 common urban roadside tree species in Espoo,Finland.Tree crown structure information,intensity features,and spectral data were used for classification.Eight different machine learning algorithms were tested,with the extra trees(ET)algorithm performing the best,achieving an overall accuracy of 71.7%using multispectral LiDAR data.This result highlights that integrating structural and spectral information within a single framework can improve the classification accuracy.Future research will focus on identifying the most important features for species classification and developing algorithms with greater efficiency and accuracy. 展开更多
关键词 multispectral airborne LiDAR machine learning tree species classification
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CCDC80在恶性肿瘤中的机制研究进展及临床意义
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作者 苏亚斯 石喆 戴文斌 《中国现代医生》 2025年第27期112-116,共5页
含卷曲螺旋结构域蛋白(coiled-coil domain containing,CCDC)80是一种可编码蛋白,在多种人体组织中广泛表达。研究发现CCDC80在多种关键生理过程中均发挥重要作用,且与恶性肿瘤密切相关,呈现抑癌或促癌的双向作用。因此,探究CCDC80在恶... 含卷曲螺旋结构域蛋白(coiled-coil domain containing,CCDC)80是一种可编码蛋白,在多种人体组织中广泛表达。研究发现CCDC80在多种关键生理过程中均发挥重要作用,且与恶性肿瘤密切相关,呈现抑癌或促癌的双向作用。因此,探究CCDC80在恶性肿瘤中的作用机制及临床意义,对攻克人类恶性肿瘤和研发相关靶向药物具有重要意义。本综述重点阐述CCDC80在恶性肿瘤中发挥各种生物学功能的分子机制和临床意义,以期为CCDC80介导肿瘤发生发展分子机制的深入研究和相关防治药物的研发提供新的思路和见解。 展开更多
关键词 含卷曲螺旋结构域蛋白80 ccdc80 恶性肿瘤 分子机制
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lncRNA CCDC183-AS1调节miR-628-5p/KLF12信号轴对宫颈癌细胞恶性生物学行为的影响
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作者 王瑾 高倩 +1 位作者 刁丛 刘蓬 《中国优生与遗传杂志》 2025年第5期1040-1046,共7页
目的该研究旨在探讨长链非编码RNA(lncRNA)CCDC183-AS1调节miR-628-5p/KLF转录因子12(KLF12)信号通路对宫颈癌细胞恶性生物学行为的影响。方法对宫颈癌细胞(CaSki、HeLa、SiHa、MS751)进行表型筛选,选择CaSki细胞分为si-NC组、si-CCDC18... 目的该研究旨在探讨长链非编码RNA(lncRNA)CCDC183-AS1调节miR-628-5p/KLF转录因子12(KLF12)信号通路对宫颈癌细胞恶性生物学行为的影响。方法对宫颈癌细胞(CaSki、HeLa、SiHa、MS751)进行表型筛选,选择CaSki细胞分为si-NC组、si-CCDC183-AS1组、mimic NC组、miR-628-5p mimic组、si-CCDC183-AS1+inhibitor NC组、si-CCDC183-AS1+miR-628-5p inhibitor组。qRT-PCR检测lncRNA CCDC183-AS1、miR-628-5p、KLF12 mRNA表达水平;EdU检测CaSki细胞增殖;Transwell检测CaSki细胞迁移和侵袭情况;流式检测CaSki细胞凋亡率;Western blot检测KLF12蛋白表达;双荧光素酶检测lncRNA CCDC183-AS1与miR-628-5p、miR-628-5p与KLF12的相互关系。结果与人正常宫颈细胞相比,宫颈癌细胞(CaSki、HeLa、SiHa、MS751)miR-628-5p表达降低,CCDC183-AS1和KLF12 mRNA表达提高,CaSki细胞表型变化最明显(P<0.05);与si-NC组比较,si-CCDC183-AS1组CaSki中CCDC183-AS1和KLF12表达、细胞增殖、迁移和侵袭能力降低,miR-628-5p表达和凋亡率升高(P<0.05);与si-CCDC183-AS1+inhibitor NC组比较,si-CCDC183-AS1+miR-628-5p inhibitor组CaSki中KLF12表达、细胞增殖、迁移和侵袭能力提高,miR-628-5p表达和凋亡率降低(P<0.05);双荧光素酶检测显示,lncRNA CCDC183-AS1与miR-628-5p、miR-628-5p与KLF12存在靶向关系(P<0.05)。结论lncRNA CCDC183-AS1可能通过抑制miR-628-5p,促进KLF12表达,促进宫颈癌细胞恶性生物学行为。 展开更多
关键词 lncRNA ccdc183-AS1 miR-628-5p KLF12 宫颈癌细胞 恶性生物学行为
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Nondestructive detection and classification of impurities-containing seed cotton based on hyperspectral imaging and one-dimensional convolutional neural network 被引量:1
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作者 Yeqi Fei Zhenye Li +2 位作者 Tingting Zhu Zengtao Chen Chao Ni 《Digital Communications and Networks》 2025年第2期308-316,共9页
The cleanliness of seed cotton plays a critical role in the pre-treatment of cotton textiles,and the removal of impurity during the harvesting process directly determines the quality and market value of cotton textile... The cleanliness of seed cotton plays a critical role in the pre-treatment of cotton textiles,and the removal of impurity during the harvesting process directly determines the quality and market value of cotton textiles.By fusing band combination optimization with deep learning,this study aims to achieve more efficient and accurate detection of film impurities in seed cotton on the production line.By applying hyperspectral imaging and a one-dimensional deep learning algorithm,we detect and classify impurities in seed cotton after harvest.The main categories detected include pure cotton,conveyor belt,film covering seed cotton,and film adhered to the conveyor belt.The proposed method achieves an impurity detection rate of 99.698%.To further ensure the feasibility and practical application potential of this strategy,we compare our results against existing mainstream methods.In addition,the model shows excellent recognition performance on pseudo-color images of real samples.With a processing time of 11.764μs per pixel from experimental data,it shows a much improved speed requirement while maintaining the accuracy of real production lines.This strategy provides an accurate and efficient method for removing impurities during cotton processing. 展开更多
关键词 Seed cotton Film impurity Hyperspectral imaging Band optimization classification
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Multi-Scale Dilated Convolution Network for SPECT-MPI Cardiovascular Disease Classification with Adaptive Denoising and Attenuation Correction
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作者 A.Robert Singh Suganya Athisayamani +1 位作者 Gyanendra Prasad Joshi Bhanu Shrestha 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期299-327,共29页
Myocardial perfusion imaging(MPI),which uses single-photon emission computed tomography(SPECT),is a well-known estimating tool for medical diagnosis,employing the classification of images to show situations in coronar... Myocardial perfusion imaging(MPI),which uses single-photon emission computed tomography(SPECT),is a well-known estimating tool for medical diagnosis,employing the classification of images to show situations in coronary artery disease(CAD).The automatic classification of SPECT images for different techniques has achieved near-optimal accuracy when using convolutional neural networks(CNNs).This paper uses a SPECT classification framework with three steps:1)Image denoising,2)Attenuation correction,and 3)Image classification.Image denoising is done by a U-Net architecture that ensures effective image denoising.Attenuation correction is implemented by a convolution neural network model that can remove the attenuation that affects the feature extraction process of classification.Finally,a novel multi-scale diluted convolution(MSDC)network is proposed.It merges the features extracted in different scales and makes the model learn the features more efficiently.Three scales of filters with size 3×3 are used to extract features.All three steps are compared with state-of-the-art methods.The proposed denoising architecture ensures a high-quality image with the highest peak signal-to-noise ratio(PSNR)value of 39.7.The proposed classification method is compared with the five different CNN models,and the proposed method ensures better classification with an accuracy of 96%,precision of 87%,sensitivity of 87%,specificity of 89%,and F1-score of 87%.To demonstrate the importance of preprocessing,the classification model was analyzed without denoising and attenuation correction. 展开更多
关键词 SPECT-MPI CAD MSDC DENOISING attenuation correction classification
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Various classification methods for diabetes mellitus in the management of blood glucose control 被引量:1
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作者 Qing Jiang Yun Hu Jian-Hua Ma 《World Journal of Diabetes》 2025年第5期1-7,共7页
In the era of precision medicine,the classification of diabetes mellitus has evolved beyond the traditional categories.Various classification methods now account for a multitude of factors,including variations in spec... In the era of precision medicine,the classification of diabetes mellitus has evolved beyond the traditional categories.Various classification methods now account for a multitude of factors,including variations in specific genes,type ofβ-cell impairment,degree of insulin resistance,and clinical characteristics of metabolic profiles.Improved classification methods enable healthcare providers to formulate blood glucose management strategies more precisely.Applying these updated classification systems,will assist clinicians in further optimising treatment plans,including targeted drug therapies,personalized dietary advice,and specific exercise plans.Ultimately,this will facilitate stricter blood glucose control,minimize the risks of hypoglycaemia and hyperglycaemia,and reduce long-term complications associated with diabetes. 展开更多
关键词 Diabetes classification Glycaemic control Personalised treatment Soft clustering Precision medicine
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Three-Stage Transfer Learning with AlexNet50 for MRI Image Multi-Class Classification with Optimal Learning Rate
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作者 Suganya Athisayamani A.Robert Singh +1 位作者 Gyanendra Prasad Joshi Woong Cho 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期155-183,共29页
In radiology,magnetic resonance imaging(MRI)is an essential diagnostic tool that provides detailed images of a patient’s anatomical and physiological structures.MRI is particularly effective for detecting soft tissue... In radiology,magnetic resonance imaging(MRI)is an essential diagnostic tool that provides detailed images of a patient’s anatomical and physiological structures.MRI is particularly effective for detecting soft tissue anomalies.Traditionally,radiologists manually interpret these images,which can be labor-intensive and time-consuming due to the vast amount of data.To address this challenge,machine learning,and deep learning approaches can be utilized to improve the accuracy and efficiency of anomaly detection in MRI scans.This manuscript presents the use of the Deep AlexNet50 model for MRI classification with discriminative learning methods.There are three stages for learning;in the first stage,the whole dataset is used to learn the features.In the second stage,some layers of AlexNet50 are frozen with an augmented dataset,and in the third stage,AlexNet50 with an augmented dataset with the augmented dataset.This method used three publicly available MRI classification datasets:Harvard whole brain atlas(HWBA-dataset),the School of Biomedical Engineering of Southern Medical University(SMU-dataset),and The National Institute of Neuroscience and Hospitals brain MRI dataset(NINS-dataset)for analysis.Various hyperparameter optimizers like Adam,stochastic gradient descent(SGD),Root mean square propagation(RMS prop),Adamax,and AdamW have been used to compare the performance of the learning process.HWBA-dataset registers maximum classification performance.We evaluated the performance of the proposed classification model using several quantitative metrics,achieving an average accuracy of 98%. 展开更多
关键词 MRI TUMORS classification AlexNet50 transfer learning hyperparameter tuning OPTIMIZER
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TMC-GCN: Encrypted Traffic Mapping Classification Method Based on Graph Convolutional Networks 被引量:1
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作者 Baoquan Liu Xi Chen +2 位作者 Qingjun Yuan Degang Li Chunxiang Gu 《Computers, Materials & Continua》 2025年第2期3179-3201,共23页
With the emphasis on user privacy and communication security, encrypted traffic has increased dramatically, which brings great challenges to traffic classification. The classification method of encrypted traffic based... With the emphasis on user privacy and communication security, encrypted traffic has increased dramatically, which brings great challenges to traffic classification. The classification method of encrypted traffic based on GNN can deal with encrypted traffic well. However, existing GNN-based approaches ignore the relationship between client or server packets. In this paper, we design a network traffic topology based on GCN, called Flow Mapping Graph (FMG). FMG establishes sequential edges between vertexes by the arrival order of packets and establishes jump-order edges between vertexes by connecting packets in different bursts with the same direction. It not only reflects the time characteristics of the packet but also strengthens the relationship between the client or server packets. According to FMG, a Traffic Mapping Classification model (TMC-GCN) is designed, which can automatically capture and learn the characteristics and structure information of the top vertex in FMG. The TMC-GCN model is used to classify the encrypted traffic. The encryption stream classification problem is transformed into a graph classification problem, which can effectively deal with data from different data sources and application scenarios. By comparing the performance of TMC-GCN with other classical models in four public datasets, including CICIOT2023, ISCXVPN2016, CICAAGM2017, and GraphDapp, the effectiveness of the FMG algorithm is verified. The experimental results show that the accuracy rate of the TMC-GCN model is 96.13%, the recall rate is 95.04%, and the F1 rate is 94.54%. 展开更多
关键词 Encrypted traffic classification deep learning graph neural networks multi-layer perceptron graph convolutional networks
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A novel method for clustering cellular data to improve classification
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作者 Diek W.Wheeler Giorgio A.Ascoli 《Neural Regeneration Research》 SCIE CAS 2025年第9期2697-2705,共9页
Many fields,such as neuroscience,are experiencing the vast prolife ration of cellular data,underscoring the need fo r organizing and interpreting large datasets.A popular approach partitions data into manageable subse... Many fields,such as neuroscience,are experiencing the vast prolife ration of cellular data,underscoring the need fo r organizing and interpreting large datasets.A popular approach partitions data into manageable subsets via hierarchical clustering,but objective methods to determine the appropriate classification granularity are missing.We recently introduced a technique to systematically identify when to stop subdividing clusters based on the fundamental principle that cells must differ more between than within clusters.Here we present the corresponding protocol to classify cellular datasets by combining datadriven unsupervised hierarchical clustering with statistical testing.These general-purpose functions are applicable to any cellular dataset that can be organized as two-dimensional matrices of numerical values,including molecula r,physiological,and anatomical datasets.We demonstrate the protocol using cellular data from the Janelia MouseLight project to chara cterize morphological aspects of neurons. 展开更多
关键词 cellular data clustering dendrogram data classification Levene's one-tailed statistical test unsupervised hierarchical clustering
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Congruent Feature Selection Method to Improve the Efficacy of Machine Learning-Based Classification in Medical Image Processing
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作者 Mohd Anjum Naoufel Kraiem +2 位作者 Hong Min Ashit Kumar Dutta Yousef Ibrahim Daradkeh 《Computer Modeling in Engineering & Sciences》 SCIE EI 2025年第1期357-384,共28页
Machine learning(ML)is increasingly applied for medical image processing with appropriate learning paradigms.These applications include analyzing images of various organs,such as the brain,lung,eye,etc.,to identify sp... Machine learning(ML)is increasingly applied for medical image processing with appropriate learning paradigms.These applications include analyzing images of various organs,such as the brain,lung,eye,etc.,to identify specific flaws/diseases for diagnosis.The primary concern of ML applications is the precise selection of flexible image features for pattern detection and region classification.Most of the extracted image features are irrelevant and lead to an increase in computation time.Therefore,this article uses an analytical learning paradigm to design a Congruent Feature Selection Method to select the most relevant image features.This process trains the learning paradigm using similarity and correlation-based features over different textural intensities and pixel distributions.The similarity between the pixels over the various distribution patterns with high indexes is recommended for disease diagnosis.Later,the correlation based on intensity and distribution is analyzed to improve the feature selection congruency.Therefore,the more congruent pixels are sorted in the descending order of the selection,which identifies better regions than the distribution.Now,the learning paradigm is trained using intensity and region-based similarity to maximize the chances of selection.Therefore,the probability of feature selection,regardless of the textures and medical image patterns,is improved.This process enhances the performance of ML applications for different medical image processing.The proposed method improves the accuracy,precision,and training rate by 13.19%,10.69%,and 11.06%,respectively,compared to other models for the selected dataset.The mean error and selection time is also reduced by 12.56%and 13.56%,respectively,compared to the same models and dataset. 展开更多
关键词 Computer vision feature selection machine learning region detection texture analysis image classification medical images
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NUP98::CCDC28A融合基因阳性成人早期前体T细胞急性淋巴细胞白血病1例
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作者 刘邵梅 陈平 +3 位作者 刘要伟 董丽丽 窦立萍 黄赛 《中国肿瘤临床》 北大核心 2025年第14期755-756,共2页
患者女性,22岁,主诉双侧颜面浮肿伴颌下淋巴结肿大、发热6个月余。2021年12月患者就诊于解放军总医院第一医学中心。CT检查示:纵隔内、双侧腋窝及双侧腹股沟多发肿大淋巴结,前纵隔软组织密度影,盆腔少量积液;超声检查示:双侧颈部及锁骨... 患者女性,22岁,主诉双侧颜面浮肿伴颌下淋巴结肿大、发热6个月余。2021年12月患者就诊于解放军总医院第一医学中心。CT检查示:纵隔内、双侧腋窝及双侧腹股沟多发肿大淋巴结,前纵隔软组织密度影,盆腔少量积液;超声检查示:双侧颈部及锁骨上窝、双侧腋下及双侧腹股沟可见多发低回声结节;淋巴结穿刺病理示:结合形态学表现及免疫组化,符合T淋巴母细胞淋巴瘤;骨髓细胞形态学意见:多考虑淋巴瘤/白血病骨髓象,白血病细胞形态,见图1;骨髓免疫分型结果示:表型异常的幼稚T细胞占28.16%,表达CD7、CD38、CD33,少量细胞表达CD5、HLA-DR. 展开更多
关键词 早期前体T 细胞急性淋巴细胞白血病 融合基因 NUP98::ccdc28A
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New classification of gastric polyps:An in-depth analysis and critical evaluation 被引量:1
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作者 Xiao-Hui Liao Ying-Ming Sun Hong-Bin Chen 《World Journal of Gastroenterology》 2025年第7期149-155,共7页
With the widespread use of upper gastrointestinal endoscopy,more and more gastric polyps(GPs)are being detected.Traditional management strategies often rely on histopathologic examination,which can be time-consuming a... With the widespread use of upper gastrointestinal endoscopy,more and more gastric polyps(GPs)are being detected.Traditional management strategies often rely on histopathologic examination,which can be time-consuming and may not guide immediate clinical decisions.This paper aims to introduce a novel classification system for GPs based on their potential risk of malignant transformation,categorizing them as"good","bad",and"ugly".A review of the literature and clinical case analysis were conducted to explore the clinical implications,management strategies,and the system's application in endoscopic practice.Good polyps,mainly including fundic gland polyps and inflammatory fibrous polyps,have a low risk of malignancy and typically require minimal or no intervention.Bad polyps,mainly including hyperplastic polyps and adenomas,pose an intermediate risk of malignancy,necessitating closer monitoring or removal.Ugly polyps,mainly including type 3 neuroendocrine tumors and early gastric cancer,indicate a high potential for malignancy and require urgent and comprehensive treatment.The new classification system provides a simplified and practical framework for diagnosing and managing GPs,improving diagnostic accuracy,guiding individualized treatment,and promoting advancements in endoscopic techniques.Despite some challenges,such as the risk of misclassification due to similar endoscopic appearances,this system is essential for the standardized management of GPs.It also lays the foundation for future research into biomarkers and the development of personalized medicine. 展开更多
关键词 Gastric polyps classification Fundic gland polyps Inflammatory fibroid polyps Hyperplastic polyps ADENOMAS Neuroendocrine tumors Early gastric cancer Patient management
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Infrared aircraft few-shot classification method based on cross-correlation network
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作者 HUANG Zhen ZHANG Yong GONG Jin-Fu 《红外与毫米波学报》 北大核心 2025年第1期103-111,共9页
In response to the scarcity of infrared aircraft samples and the tendency of traditional deep learning to overfit,a few-shot infrared aircraft classification method based on cross-correlation networks is proposed.This... In response to the scarcity of infrared aircraft samples and the tendency of traditional deep learning to overfit,a few-shot infrared aircraft classification method based on cross-correlation networks is proposed.This method combines two core modules:a simple parameter-free self-attention and cross-attention.By analyzing the self-correlation and cross-correlation between support images and query images,it achieves effective classification of infrared aircraft under few-shot conditions.The proposed cross-correlation network integrates these two modules and is trained in an end-to-end manner.The simple parameter-free self-attention is responsible for extracting the internal structure of the image while the cross-attention can calculate the cross-correlation between images further extracting and fusing the features between images.Compared with existing few-shot infrared target classification models,this model focuses on the geometric structure and thermal texture information of infrared images by modeling the semantic relevance between the features of the support set and query set,thus better attending to the target objects.Experimental results show that this method outperforms existing infrared aircraft classification methods in various classification tasks,with the highest classification accuracy improvement exceeding 3%.In addition,ablation experiments and comparative experiments also prove the effectiveness of the method. 展开更多
关键词 infrared imaging aircraft classification few-shot learning parameter-free attention cross attention
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Retraction:Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203
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作者 Oncology Research Editorial Office 《Oncology Research》 2025年第10期3156-3156,共1页
The published article titled“Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203”has been retracted fromOncology Research,Vol.26,No.8,2018,pp.1143... The published article titled“Silencing of lncRNA CCDC26 Restrains the Growth and Migration of Glioma Cells In Vitro and In Vivo via Targeting miR-203”has been retracted fromOncology Research,Vol.26,No.8,2018,pp.1143–1154. 展开更多
关键词 GROWTH GLIOMA glioma cells ccdc MIGRATION lncrna mir
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Audiovisual Art Event Classification and Outreach Based on Web Extracted Data
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作者 Andreas Giannakoulopoulos Minas Pergantis +1 位作者 Aristeidis Lamprogeorgos Stella Lampoura 《Journal of Software Engineering and Applications》 2025年第1期24-43,共20页
The World Wide Web provides a wealth of information about everything, including contemporary audio and visual art events, which are discussed on media outlets, blogs, and specialized websites alike. This information m... The World Wide Web provides a wealth of information about everything, including contemporary audio and visual art events, which are discussed on media outlets, blogs, and specialized websites alike. This information may become a robust source of real-world data, which may form the basis of an objective data-driven analysis. In this study, a methodology for collecting information about audio and visual art events in an automated manner from a large array of websites is presented in detail. This process uses cutting edge Semantic Web, Web Search and Generative AI technologies to convert website documents into a collection of structured data. The value of the methodology is demonstrated by creating a large dataset concerning audiovisual events in Greece. The collected information includes event characteristics, estimated metrics based on their text descriptions, outreach metrics based on the media that reported them, and a multi-layered classification of these events based on their type, subjects and methods used. This dataset is openly provided to the general and academic public through a Web application. Moreover, each event’s outreach is evaluated using these quantitative metrics, the results are analyzed with an emphasis on classification popularity and useful conclusions are drawn concerning the importance of artistic subjects, methods, and media. 展开更多
关键词 Web Data Extraction Art Events classification Artistic Outreach Online Media
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Domain-independent adaptive histogram-based features for pomegranate fruit and leaf diseases classification
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作者 Mohanmuralidhar Prajwala Prabhuswamy Prajwal Kumar +3 位作者 Shanubhog Maheshwarappa Gopinath Shivakumara Palaiahnakote Mahadevappa Basavanna Daniel P.Lopresti 《CAAI Transactions on Intelligence Technology》 2025年第2期317-336,共20页
Disease identification for fruits and leaves in the field of agriculture is important for estimating production,crop yield,and earnings for farmers.In the specific case of pomegranates,this is challenging because of t... Disease identification for fruits and leaves in the field of agriculture is important for estimating production,crop yield,and earnings for farmers.In the specific case of pomegranates,this is challenging because of the wide range of possible diseases and their effects on the plant and the crop.This study presents an adaptive histogram-based method for solving this problem.Our method describe is domain independent in the sense that it can be easily and efficiently adapted to other similar smart agriculture tasks.The approach explores colour spaces,namely,Red,Green,and Blue along with Grey.The histograms of colour spaces and grey space are analysed based on the notion that as the disease changes,the colour also changes.The proximity between the histograms of grey images with individual colour spaces is estimated to find the closeness of images.Since the grey image is the average of colour spaces(R,G,and B),it can be considered a reference image.For estimating the distance between grey and colour spaces,the proposed approach uses a Chi-Square distance measure.Further,the method uses an Artificial Neural Network for classification.The effectiveness of our approach is demonstrated by testing on a dataset of fruit and leaf images affected by different diseases.The results show that the method outperforms existing techniques in terms of average classification rate. 展开更多
关键词 color spaces distance measure fruit classification leaf classification plant disease classification
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TFAP2A对IgA肾病相关基因CCDC69转录调控机制研究
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作者 张小田 蒋文娟 +3 位作者 王梦婕 刘镇 彭洪军 任献国 《合肥医科大学学报》 2025年第10期1002-1009,共8页
目的探究转录因子TFAP2A与CCDC69启动子区特异性结合及TFAP2A对IgA肾病相关基因CCDC69的转录调控机制。方法利用GDS3712高通量数据集分析肾小球硬化肾组织RNA-Seq数据中CCDC69及TFAP2A RNA相对表达量,Trizol法提取IgAN患儿外周血TFAP2A... 目的探究转录因子TFAP2A与CCDC69启动子区特异性结合及TFAP2A对IgA肾病相关基因CCDC69的转录调控机制。方法利用GDS3712高通量数据集分析肾小球硬化肾组织RNA-Seq数据中CCDC69及TFAP2A RNA相对表达量,Trizol法提取IgAN患儿外周血TFAP2A及CCDC69 RNA,qPCR法检测RNA表达量改变,通过双萤光素酶报告基因实验验证TFAP2A对CCDC69基因启动子水平的调控作用。采用实时荧光定量PCR检测TFAP2A siRNA及TFAP2A过表达质粒转染细胞后TFAP2A、CCDC69 mRNA表达水平,采用蛋白免疫印迹检测TFAP2A、CCDC69蛋白表达水平。采用染色质免疫沉淀试验验证TFAP2A与CCDC69启动子的特定区域结合情况。结果GDS3712高通量数据集分析肾小球硬化肾组织中CCDC69表达量升高,TFAP2A表达量下降,Trizol法提取IgAN患儿外周血TFAP2A及CCDC69 RNA,qPCR法检测TFAP2A表达量下降,CCDC69表达量上升。双萤光素酶报告基因实验证明TFAP2A siRNA浓度为10、15μmol/L的CCDC69启动子相对萤光素酶活性增强(P<0.01),TFAP2A过表达质粒质量浓度为100、300μg/L的CCDC69启动子萤光素酶活性降低(P<0.05)。与Control siRNA组相比,TFAP2A siRNA组CCDC69 mRNA和蛋白表达水平升高;与pENTER质粒组比较,TFAP2A过表达质粒组CCDC69 mRNA和蛋白表达水平降低(P<0.05)。染色质免疫沉淀实验证实TFAP2A与CCDC69启动子的特定区域结合。结论转录因子TFAP2A与CCDC69启动子区特异性结合并负向调控CCDC69基因表达,本研究发现了调控IgA肾病重要基因的转录因子新成员。 展开更多
关键词 IGA肾病 TFAP2A 启动子 ccdc69 转录调控
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Variety classification and identification of maize seeds based on hyperspectral imaging method 被引量:1
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作者 XUE Hang XU Xiping MENG Xiang 《Optoelectronics Letters》 2025年第4期234-241,共8页
In this study,eight different varieties of maize seeds were used as the research objects.Conduct 81 types of combined preprocessing on the original spectra.Through comparison,Savitzky-Golay(SG)-multivariate scattering... In this study,eight different varieties of maize seeds were used as the research objects.Conduct 81 types of combined preprocessing on the original spectra.Through comparison,Savitzky-Golay(SG)-multivariate scattering correction(MSC)-maximum-minimum normalization(MN)was identified as the optimal preprocessing technique.The competitive adaptive reweighted sampling(CARS),successive projections algorithm(SPA),and their combined methods were employed to extract feature wavelengths.Classification models based on back propagation(BP),support vector machine(SVM),random forest(RF),and partial least squares(PLS)were established using full-band data and feature wavelengths.Among all models,the(CARS-SPA)-BP model achieved the highest accuracy rate of 98.44%.This study offers novel insights and methodologies for the rapid and accurate identification of corn seeds as well as other crop seeds. 展开更多
关键词 feature extraction extract feature wavelengthsclassification models variety classification hyperspectral imaging combined preprocessing competitive adaptive reweighted sampling cars successive projections algorithm spa PREPROCESSING maize seeds
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