This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using convention...This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides.展开更多
[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experime...[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experiment in a relatively small polyculture system. The agonistic behavior of P. clarkii and E. sinensis in the first 30 min of the initial encounter and the fighting results were observed and recorded; the approach-avoidance behavior of P. clarkii to bigger E. sinensis in a relatively large space （the ＂Y＂ maze） was observed and recorded. [Result] The re- sults showed that the times of first aggressions and approaches and the total times of active aggressions and approaches of P. clarkii in the initial period of polyculture experiment were all significantly more than E. sinensis （/：＇〈0.05）; after 30 d of poly- culture, P. clarkfi died in 30% of the groups while E. sinensis died in only 10% of the groups, and they coexisted in 60% of the groups. In the experiment of ap- proach-avoidance behavior of P. clarkii to bigger E. sinensis, with the increase of experiment times, the tendency of approach and avoidance of P. clarkii to the con- trol and E. sinensis became control-prefer （P〈0.05） from insignificantly different （P〉 0.05）, which indicated that the recognition ability of P. clarkii for E. sinensis was sig- nificantly improved, and the selection behavior of E. sinensis was obviously inhibited. [Conclusion] Presumably, polyculture of crayfishes and crabs can achieve better effi- ciency when under appropriate habitat conditions.展开更多
基金Supported by the Science and Technology Innovative Research Team of Anhui Academy of Agricultural Sciences(14C0504)the Youth Innovation Foundation of President of Anhui Academy of Agricultural Sciences(14B0529)Anhui Aquaculture Industry Technology System for Shrimp and Crab
文摘This experiment was conducted to clarify species and drug resistance of pathogen from the diseased Procambarus clarkia. Pathogenic bacteria from hepatopancreas of the diseased P. clarkia were examined using conventional methods,and then were isolated. The further tests and analysis of the isolated strain were developed,including the regression experiment to P. clarkia,the morphology,physiological and biochemical characteristics,sequence analysis of their 16 S rRNA and gyr B genes,and the susceptibility test to antibiotics. Large colonies with similar morphology and color were obtained. Strain X120523 was identified as Citrobacter freundii,proved to have strong pathogenicity,and was susceptible to quinolones and aminoglycosides.
基金Supported by National R&D Special Fund for Public Welfare Industry of China(201003070)National Natural Science Foundation of China (NSFC J0730641)~~
文摘[Objective] This study aimed to observe and explore the behavior of Procambarus clarkii and Eriocheir sinensis in polyculture system. [Method] P. clarkii and E. sinensis with the same weight were used for the experiment in a relatively small polyculture system. The agonistic behavior of P. clarkii and E. sinensis in the first 30 min of the initial encounter and the fighting results were observed and recorded; the approach-avoidance behavior of P. clarkii to bigger E. sinensis in a relatively large space （the ＂Y＂ maze） was observed and recorded. [Result] The re- sults showed that the times of first aggressions and approaches and the total times of active aggressions and approaches of P. clarkii in the initial period of polyculture experiment were all significantly more than E. sinensis （/：＇〈0.05）; after 30 d of poly- culture, P. clarkfi died in 30% of the groups while E. sinensis died in only 10% of the groups, and they coexisted in 60% of the groups. In the experiment of ap- proach-avoidance behavior of P. clarkii to bigger E. sinensis, with the increase of experiment times, the tendency of approach and avoidance of P. clarkii to the con- trol and E. sinensis became control-prefer （P〈0.05） from insignificantly different （P〉 0.05）, which indicated that the recognition ability of P. clarkii for E. sinensis was sig- nificantly improved, and the selection behavior of E. sinensis was obviously inhibited. [Conclusion] Presumably, polyculture of crayfishes and crabs can achieve better effi- ciency when under appropriate habitat conditions.
