In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside...In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.展开更多
[Objectives]To establish a HPLC-UV method for the determination of Cistanches Herba in Zhenrongdan mixture.[Methods]According to the content determination method of Cistanches Herba in the 2020 edition of Chinese Phar...[Objectives]To establish a HPLC-UV method for the determination of Cistanches Herba in Zhenrongdan mixture.[Methods]According to the content determination method of Cistanches Herba in the 2020 edition of Chinese Pharmacopoeia,using HPLC-UV method,the content determination method of Cistanches Herba in Zhenrongdan mixture was established,and the content of echinacoside was determined.The related chromatographic conditions were explored,and the methodological investigation was carried out.[Results]The chromatographic conditions were determined as follows:octadecylsilane bonded silica gel as filler(C_(18));80%acetonitrile solution(containing 0.2%phosphoric acid)-0.2%phosphoric acid aqueous solution(15∶85)as mobile phase;detection wavelength was 330 nm.The number of theoretical plates should not be less than 3000 according to the peak of echinacoside.The methodological investigation showed that the method had better precision,accuracy and repeatability.Under the conditions of this study,echinacoside had better linear relationship in the range of 74.9-1498 ng,and the average recovery was 99.1%,RSD=0.5%(n=6).[Conclusions]A method for the determination of Cistanches Herba in Zhenrongdan mixture by HPLC-UV was established.The method is sensitive,rapid,accurate and suitable for the determination of Cistanches Herba in Zhenrongdan mixture.展开更多
To investigate the mechanism of glycosides of cistanches improving cognitive performance dysfunction in SAMP8 mice by AMPK/mTOR signaling pathway.Male 7-month-old SAMP8 mice were randomly divided into 5 groups:model g...To investigate the mechanism of glycosides of cistanches improving cognitive performance dysfunction in SAMP8 mice by AMPK/mTOR signaling pathway.Male 7-month-old SAMP8 mice were randomly divided into 5 groups:model group,GCs group,GCs+3-MA group,GCs+compound C group,Vehicle group.Morris water maze was used to detect the ability of learning and memory.Western blot was used to detect the protein expression of SYN,PSD-95,BDNF,LC3,AMPK and m-TOR.The escape latency of GCs group,GCs+3-MA group and GCs+compound C group were significantly shortened(P<0.05).Compared with GCs group,the escape latency and percent of time spent in the target quadrant of GCs+compound C group were significantly increased(P<0.05).展开更多
OBJECTIVE:To investigate the effect of Roucongrong(Herba Cistanches Deserticolae)decoction on the substantia nigra in rats with Parkinson’s disease(PD)induced by 6-hydroxydopamine hydrochloride(6-OHDA).To further det...OBJECTIVE:To investigate the effect of Roucongrong(Herba Cistanches Deserticolae)decoction on the substantia nigra in rats with Parkinson’s disease(PD)induced by 6-hydroxydopamine hydrochloride(6-OHDA).To further determine whether the Wnt/β-catenin signaling pathway is involved in the action.METHODS:A rat model of PD was established by intracranial injection of 6-OHDA.Subsequently,three concentrations of Roucongrong(Herba Cistanches Deserticolae)decoction were prepared and administered to rats by gavage therapy for 14 d.Behavioral changes were measured in PD rats.In vivo tyrosine hydroxylase(TH)levels in the substantia nigra were examined by immunohistochemistry.Additionally,gene and protein expression levels of members of the Wnt/β-catenin signaling pathway were examined by Western blotting and polymerase chain reaction.Lastly,a Wnt/β-catenin inhibitor was used to investigate the mechanism of action in 1-methyl-4-phenylpyridinium(MPP+)-treated MES23.5 cells in vitro.RESULTS:Roucongrong(Herba Cistanches Deserticolae)decoction improved performance in the stride and gait adjustment tests in PD rats.It also increased TH in the substantia nigra and altered the expression of genes and proteins in the Wnt/β-catenin signaling pathway.Wnt/β-catenin inhibitor reduced the effect of Roucongrong(Herba Cistanches Deserticolae)decoction in MPP+-treated MES23.5 cells.CONCLUSION:Roucongrong(Herba Cistanches Deserticolae)decoction may promote neuronal survival in PD in vivo and in vitro by increasing TH content in the substantia nigra and by activating the Wnt/β-catenin signaling pathway.展开更多
OBJECTIVE： The purpose of this research is to study the effect of Roucongrong （Herba Cistanches Deserticolae） on reproductive toxicity in mice in- duced by a glycoside extracted from Leigongteng （Radix et Rhizoma ...OBJECTIVE： The purpose of this research is to study the effect of Roucongrong （Herba Cistanches Deserticolae） on reproductive toxicity in mice in- duced by a glycoside extracted from Leigongteng （Radix et Rhizoma Tripterygii） （GRT）. METHODS： Forty-eight BALB/c mice were random-ly divided into two groups in the ratio of I ： 3, 12 in one group and 36 in the other. The 12-mouse group was the control group that was intragastrical- ly administered physiological saline for 3 weeks. The 36 mice in the other group were given 30 mg- kg ·^-1 d i GRT for 3 weeks, then randomly divided into 3 subgroups： the model group, GRI group and Roucongrong （Herba Cistanches Deserticolae） group, with 12 mice in each group. In the model group, 0.25 mL physiological saline was intragastri- cally administered; in the GRT group, GRT, 0.25 mL at 30 mg · kg ^-1· d ^-1 was intragastrically adminis- tered once a day; in the Roucongrong （Herba Cis- tanches Deserticolae） group, mice were adminis- tered Roucongrong （Herba Cistanches Deserticolae） decoction equivalent to 0.25 mL at a final dose of 10g.kg 1.d.1 crude drug （calculated as per 20 times of 0.5 g· kg^-1. d ^-1 for adults）, and GRT 0.25 mL at 30 mg- kg^-1· d^- 1 daily. After another 3 weeks of exposure, expression levels of the reproduction-re- lated genes DEAD （Asp-Glu-Ala-Asp） box polypep- tide 3, Y-linked, B-cell CLL/lymphoma 6 and Signal transducer and activator of transcription 3 were evaluated. RESULTS： After 6 weeks of GRT treatment, the sper- matogenic cell population in the convoluted tu- bule of testis was in disorder and the tubule cavity expanded. Sertoli cell and Leydig cells exhibited at- rophy or disappeared. The number of sperm de- creased. The spermatogenic cell level of testis for male mice was ranked in order and sperm was pro- duced in the cavity of the spermatogenic cell. The expression levels of DDX3Y, BCL6 and STAT3 were up-regulated.CONCLUSION： GRT affected reproduction-related genes. Roucongrong （Herba Cistanches Desertico- lae） reversed reproductive toxicity in mice induced by GRT.展开更多
Parkinson' s disease(PD) is a progressive neurodegenerative disorder affecting movement,with no treatments currently available to halt or slow its progression.Therefore,the development of new anti-PD drugs is urge...Parkinson' s disease(PD) is a progressive neurodegenerative disorder affecting movement,with no treatments currently available to halt or slow its progression.Therefore,the development of new anti-PD drugs is urgently needed.As a kind of medicine and food homologous plant,Cistanches Herba has a promising future for the treatment of PD.In this study,a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced PD mouse model was used to detect Cistanches Herba' s anti-PD effects via histopathology and molecular biology.Simultaneously,the effect of Cistanches Herba on the "gut microbiota-barrier axis" was assessed through gut microbiota and intestinal barrier function in mice.Finally,transcriptomics analysis was conducted to further verify the results.As a result,37 differential metabolites and 16 microbial genera were screened and tentatively identified.Thirty-two metabolites and sixteen microbial genera were simultaneously altered with opposing trends in variation after MPTP and Cistanches Herba treatments.We built a framework for predicting targets and hostmicrobe interaction mechanisms,as well as identifying alternative treatment for PD,which should be validated further for clinical application.In conclusion,Cistanches Herba exerts a protective effect against the development of PD by manipulating the structural feature of intestinal flora to influence the host metabolites.展开更多
Objective To study the chemical constituents in the stems or Cisrancnes deserticola. Methods The compounds were purified by various column chromatographic methods and their structures were elucidated by spectroscopic ...Objective To study the chemical constituents in the stems or Cisrancnes deserticola. Methods The compounds were purified by various column chromatographic methods and their structures were elucidated by spectroscopic （1 D, 2D NMR, IR, [α]D, and MS） and chemical analyses. Results Seven compounds were isolated and identified as kankanoside D1 （1）, ajugol （2）, cistanin （3）, uridine （4）, adenosine （5）, 6-deoxycatalpol （6）, and kankanoside D （7）. Conclusion Compound 1 is a new compound.展开更多
Cistanches Herba, known as ‘‘Ginseng of the desert’’, is authenticated from the dried succulent stems of Cistanche deserticola and Cistanche tubulosa. As a famous remedy in China for tonic the kidney, it is used t...Cistanches Herba, known as ‘‘Ginseng of the desert’’, is authenticated from the dried succulent stems of Cistanche deserticola and Cistanche tubulosa. As a famous remedy in China for tonic the kidney, it is used to treat ＂kidney-deficiency syndrome＂-induced diseases such as infertility, forgetfulness, hearing lost, chronic constipation, etc.. As various biological activities, including anti-aging, antioxidant, estrogenic, anti-osteoporotic, and anti- inflammation effects, have been discovered, here we reviewed Cistanches Herba in biological characteristics, chemical constituents, and pharmacological activities.展开更多
Background:This study aimed to discover whether Cistanche tubulosa affects the AKT/CASP3 pathway by regulating m6A methylation,to exert a protective effect against peripheral nerve injury in a Parkinson's Disease(...Background:This study aimed to discover whether Cistanche tubulosa affects the AKT/CASP3 pathway by regulating m6A methylation,to exert a protective effect against peripheral nerve injury in a Parkinson's Disease(PD)mouse model.Methods:In this study,network pharmacology analysis and the molecular docking virtual screening technique was used to filter Acteoside(Act),a potential neuroprotective agent of active components in Cistanche tubulosa.A PD-related peripheral neuropathy mouse model was established by MPTP induction,followed by 21 days treatment of oral Act(25,50,and 100 mg kg^(−1)).Pole climbing,automatic avoidance ability and hot plate sensory tests were evaluated to determine behavioral changes caused by central and peripheral nerve injury.The pathological alterations of dorsal root ganglion tissue and the protein levels of IL-6,AKT,and CASP3 under Act intervention,as well as the dynamic changes of FTO,METTL3,and YTHDF2 which are closely related to m6A methylation,were comprehensively analyzed to observe the peripheral nerve protective efficacy of Act.Results:The results showed that peripheral neuropathy occurring with PD in the mouse model,which could be verified by behavioral tests and pathological histological changes.In addition to the previously established protective effect of Act on dopaminergic neurons in substantia nigra(SN),extensive follow-up studies demonstrated that Act effectively induced m6A methylation,which could further regulate the AKT/CASP3 pathway to play a therapeutic role.In this study,medium and high doses of Act played more obvious therapeutic roles.Conclusion:These findings suggest that Act may regulate the severity of peripheral nerve injury under the activation of the AKT/CASP3 signaling pathway by balancing the methylation level of m6A.These results provide a theoretical basis and experimental evidence for further research on the protective effect of Cistanche tubulosa on both the central and peripheral nerves in the treatment of PD.展开更多
Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The d...Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.展开更多
Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubat...Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products （CB-1, CB-2） from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method.展开更多
BACKGROUND Constipation is one of the chronic gastrointestinal functional diseases.It seriously affects the quality of life.Cistanche deserticola(C.deserticola)can treat constipation obviously,but its mechanism has no...BACKGROUND Constipation is one of the chronic gastrointestinal functional diseases.It seriously affects the quality of life.Cistanche deserticola(C.deserticola)can treat constipation obviously,but its mechanism has not been clarified.We supposed that mechanism of it improved the intestinal motility by stimulating interstitial Cajal cells(ICC).Activation of the C-kit receptor on the surface of ICC is closely related to ICC function,and the stem cell factor(SCF)/C-kit signaling pathways plays an important role on it.To investigate the mechanism of how C.deserticola treats constipation,this study aimed to establish a constipation model in rats and explore the role of SCF/C-kit signaling pathway in the treatment.AIM To explore the SCF/C-kit signaling pathways in the role of C.deserticola for treatment of constipation by a constipation rat model.METHODS Forty-eight 8-mo-old Sprague–Dawley rats were divided into 4 groups by random weight method:Normal group(n=12),model group(n=12),C.deserticola group(n=12)and blocker group(n=12).The normal group received normal saline by gavage;the model group received loperamide by gavage;the blocker group received loperamide and C.deserticola by gavage,and STI571 was injected by intraperitoneally.During treatment,the weight,fecal granules and fecal quality were recorded every 10 d.On day 20 after model induction,the colon tissues of each group were removed.Hematoxylin and eosin staining was used to observe pathological changes.Expression levels of SCF,C-kit and Aquaporin genes were detected by immunohistochemistry,western blotting,and real-time-quantitative polymerase chain reaction.The colonic epithelial mitochondria and goblet cells were observed by transmission electron microscopy.RESULTS Compared with the normal group,as treatment progressed,the weight of rats in the model and blocker groups decreased significantly,the stool weight decreased,and the stool quality was dry(P<0.05).C.deserticola reversed the decrease in body weight and stool weight and improved stool quality.Histopathological analysis indicated that the colonic mucosal epithelium in the model group was incomplete,and the arrangement of the glands was irregular or damaged.Treatment with C.deserticola improved the integrity and continuity of the epithelial cells and regular arrangement of the glands.The blocking agents inhibited the effects of C.deserticola Immunohistochemistry and real-timequantitative polymerase chain reaction showed that expression of SCF and C-kit protein or genes in the colonic tissue of the model group was decreased(P<0.05),while treatment with C.deserticola increased protein or gene expression(P<0.05).Western blotting showed that expression of aquaporin APQ3 was increased,while the expression of Cx43 decreased in the model group.Treatment with C.deserticola inhibited expression of APQ3 and promoted expression of Cx43.Transmission electron microscopy showed that the mitochondria of the colonic epithelium in the model group were swollen and arranged disorderly,and microvilli were sparse.The condition was better in the C.deserticola group.Mice treated with STI571 blocker confirmed that blocking the SCF/C-kit pathway inhibited the improvement of constipation by C.deserticola.CONCLUSION C.deserticola improved defecation in rats with constipation,and the SCF/C-kit signaling pathway,which is a key link of ICC function,played an important role of the treatment.展开更多
基金National Key R&D Program of China(Grant Nos.2017YFC1702400,2018YFC1707300 and 2018YFC1707904)
文摘In the present study, a method for the quantitative analysis of multi-components by single marker(QAMS) has been developed and validated for the simultaneous determination of echinacoside(ECH), tubuloside A, acteoside, isoacteoside, and2’-acetylacteoside in Cistanches Herba. ECH was used as the internal standard(IS) to obtain the relative correction factors(RCFs) of the other four phenylethanoid glycosides(PhGs);meanwhile, various influencing factors on RCFs were investigated under different conditions. The content of each component was calculated with RCF. The results were compared with those obtained by the external standard method(ESM) to verify the feasibility and accuracy of the established QAMS method. No significant difference was found in the quantitative results of 10 batches of Cistanches Herba between QAMS and ESM. The proposed QAMS method for simultaneous determination of PhGs in Cistanches Herba is accurate and feasible, providing an efficient and economical approach for the quality control of Cistanches Herba.
文摘[Objectives]To establish a HPLC-UV method for the determination of Cistanches Herba in Zhenrongdan mixture.[Methods]According to the content determination method of Cistanches Herba in the 2020 edition of Chinese Pharmacopoeia,using HPLC-UV method,the content determination method of Cistanches Herba in Zhenrongdan mixture was established,and the content of echinacoside was determined.The related chromatographic conditions were explored,and the methodological investigation was carried out.[Results]The chromatographic conditions were determined as follows:octadecylsilane bonded silica gel as filler(C_(18));80%acetonitrile solution(containing 0.2%phosphoric acid)-0.2%phosphoric acid aqueous solution(15∶85)as mobile phase;detection wavelength was 330 nm.The number of theoretical plates should not be less than 3000 according to the peak of echinacoside.The methodological investigation showed that the method had better precision,accuracy and repeatability.Under the conditions of this study,echinacoside had better linear relationship in the range of 74.9-1498 ng,and the average recovery was 99.1%,RSD=0.5%(n=6).[Conclusions]A method for the determination of Cistanches Herba in Zhenrongdan mixture by HPLC-UV was established.The method is sensitive,rapid,accurate and suitable for the determination of Cistanches Herba in Zhenrongdan mixture.
文摘To investigate the mechanism of glycosides of cistanches improving cognitive performance dysfunction in SAMP8 mice by AMPK/mTOR signaling pathway.Male 7-month-old SAMP8 mice were randomly divided into 5 groups:model group,GCs group,GCs+3-MA group,GCs+compound C group,Vehicle group.Morris water maze was used to detect the ability of learning and memory.Western blot was used to detect the protein expression of SYN,PSD-95,BDNF,LC3,AMPK and m-TOR.The escape latency of GCs group,GCs+3-MA group and GCs+compound C group were significantly shortened(P<0.05).Compared with GCs group,the escape latency and percent of time spent in the target quadrant of GCs+compound C group were significantly increased(P<0.05).
基金Supported by the Creation of Major New Drugs for Major National Science and Technology Projects(No.2019ZX09301154)National Natural Science Foundation of China(Nos.81904263,81804156)+1 种基金2021 Fujian Provincial Traditional Chinese Medicine Science and Technology Program2018 Outstanding Young Scientific Research Project in Fujian province。
文摘OBJECTIVE:To investigate the effect of Roucongrong(Herba Cistanches Deserticolae)decoction on the substantia nigra in rats with Parkinson’s disease(PD)induced by 6-hydroxydopamine hydrochloride(6-OHDA).To further determine whether the Wnt/β-catenin signaling pathway is involved in the action.METHODS:A rat model of PD was established by intracranial injection of 6-OHDA.Subsequently,three concentrations of Roucongrong(Herba Cistanches Deserticolae)decoction were prepared and administered to rats by gavage therapy for 14 d.Behavioral changes were measured in PD rats.In vivo tyrosine hydroxylase(TH)levels in the substantia nigra were examined by immunohistochemistry.Additionally,gene and protein expression levels of members of the Wnt/β-catenin signaling pathway were examined by Western blotting and polymerase chain reaction.Lastly,a Wnt/β-catenin inhibitor was used to investigate the mechanism of action in 1-methyl-4-phenylpyridinium(MPP+)-treated MES23.5 cells in vitro.RESULTS:Roucongrong(Herba Cistanches Deserticolae)decoction improved performance in the stride and gait adjustment tests in PD rats.It also increased TH in the substantia nigra and altered the expression of genes and proteins in the Wnt/β-catenin signaling pathway.Wnt/β-catenin inhibitor reduced the effect of Roucongrong(Herba Cistanches Deserticolae)decoction in MPP+-treated MES23.5 cells.CONCLUSION:Roucongrong(Herba Cistanches Deserticolae)decoction may promote neuronal survival in PD in vivo and in vitro by increasing TH content in the substantia nigra and by activating the Wnt/β-catenin signaling pathway.
基金Supported by Ministry of Scientific Technology of China Grant(No.201007005)National Natural Science Foundation of China Grant(No.81173219)+3 种基金Shanghai Science & Technology Commission Grant(No.11DZ1973100No.12ZR1432400)Key Clinic Laboratory of TCM of Shanghai,the Chinese Medicine Development Three Years Project of Shanghai Municipal Health Bureau(No.ZYSNXD-CC-YJXYY)Innovative Research Team in Universities,Shanghai Municipal Education
文摘OBJECTIVE： The purpose of this research is to study the effect of Roucongrong （Herba Cistanches Deserticolae） on reproductive toxicity in mice in- duced by a glycoside extracted from Leigongteng （Radix et Rhizoma Tripterygii） （GRT）. METHODS： Forty-eight BALB/c mice were random-ly divided into two groups in the ratio of I ： 3, 12 in one group and 36 in the other. The 12-mouse group was the control group that was intragastrical- ly administered physiological saline for 3 weeks. The 36 mice in the other group were given 30 mg- kg ·^-1 d i GRT for 3 weeks, then randomly divided into 3 subgroups： the model group, GRI group and Roucongrong （Herba Cistanches Deserticolae） group, with 12 mice in each group. In the model group, 0.25 mL physiological saline was intragastri- cally administered; in the GRT group, GRT, 0.25 mL at 30 mg · kg ^-1· d ^-1 was intragastrically adminis- tered once a day; in the Roucongrong （Herba Cis- tanches Deserticolae） group, mice were adminis- tered Roucongrong （Herba Cistanches Deserticolae） decoction equivalent to 0.25 mL at a final dose of 10g.kg 1.d.1 crude drug （calculated as per 20 times of 0.5 g· kg^-1. d ^-1 for adults）, and GRT 0.25 mL at 30 mg- kg^-1· d^- 1 daily. After another 3 weeks of exposure, expression levels of the reproduction-re- lated genes DEAD （Asp-Glu-Ala-Asp） box polypep- tide 3, Y-linked, B-cell CLL/lymphoma 6 and Signal transducer and activator of transcription 3 were evaluated. RESULTS： After 6 weeks of GRT treatment, the sper- matogenic cell population in the convoluted tu- bule of testis was in disorder and the tubule cavity expanded. Sertoli cell and Leydig cells exhibited at- rophy or disappeared. The number of sperm de- creased. The spermatogenic cell level of testis for male mice was ranked in order and sperm was pro- duced in the cavity of the spermatogenic cell. The expression levels of DDX3Y, BCL6 and STAT3 were up-regulated.CONCLUSION： GRT affected reproduction-related genes. Roucongrong （Herba Cistanches Desertico- lae） reversed reproductive toxicity in mice induced by GRT.
基金supported by the National Natural Science Foundation of China (82204375,81903657,82003933)the Science and Technology Development Planning Project of Traditional Chinese Medicine of Jiangsu Province of China (QN202417,QN202103)+9 种基金Supporting Projects of Youth Science Foundation of National Natural Science Foundation of China (XPT82204375)333High-level Talent Development Project in Jiangsu Province ((2024)3-2394)Supporting Projects of Natural Science Foundation of Jiangsu Province (PT20240402)Research Project of Jiangsu Association of Chinese Medicine (CYTF2024021)Jiangsu Science and Technology Association Young Science and Technology Talents Lifting Project(JSTJ-2024-343)Natural Science Foundation of Nanjing University of Chinese Medicine (XZR2021046)the Nanjing Youth Talent Training Plan of TCM (ZYQ20006)NATCM's Project of High-level Construction of Key TCM DisciplinesNanjing University of Chinese Medicine Foundation Youth Project (NZY903657)the Research Project of Jiangsu Commission of Health (Z2023057)。
文摘Parkinson' s disease(PD) is a progressive neurodegenerative disorder affecting movement,with no treatments currently available to halt or slow its progression.Therefore,the development of new anti-PD drugs is urgently needed.As a kind of medicine and food homologous plant,Cistanches Herba has a promising future for the treatment of PD.In this study,a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)-induced PD mouse model was used to detect Cistanches Herba' s anti-PD effects via histopathology and molecular biology.Simultaneously,the effect of Cistanches Herba on the "gut microbiota-barrier axis" was assessed through gut microbiota and intestinal barrier function in mice.Finally,transcriptomics analysis was conducted to further verify the results.As a result,37 differential metabolites and 16 microbial genera were screened and tentatively identified.Thirty-two metabolites and sixteen microbial genera were simultaneously altered with opposing trends in variation after MPTP and Cistanches Herba treatments.We built a framework for predicting targets and hostmicrobe interaction mechanisms,as well as identifying alternative treatment for PD,which should be validated further for clinical application.In conclusion,Cistanches Herba exerts a protective effect against the development of PD by manipulating the structural feature of intestinal flora to influence the host metabolites.
基金Important Drug Development Fund,Ministry of Science and Technology of China(2015ZX09501004-003-004)
文摘Objective To study the chemical constituents in the stems or Cisrancnes deserticola. Methods The compounds were purified by various column chromatographic methods and their structures were elucidated by spectroscopic （1 D, 2D NMR, IR, [α]D, and MS） and chemical analyses. Results Seven compounds were isolated and identified as kankanoside D1 （1）, ajugol （2）, cistanin （3）, uridine （4）, adenosine （5）, 6-deoxycatalpol （6）, and kankanoside D （7）. Conclusion Compound 1 is a new compound.
文摘Cistanches Herba, known as ‘‘Ginseng of the desert’’, is authenticated from the dried succulent stems of Cistanche deserticola and Cistanche tubulosa. As a famous remedy in China for tonic the kidney, it is used to treat ＂kidney-deficiency syndrome＂-induced diseases such as infertility, forgetfulness, hearing lost, chronic constipation, etc.. As various biological activities, including anti-aging, antioxidant, estrogenic, anti-osteoporotic, and anti- inflammation effects, have been discovered, here we reviewed Cistanches Herba in biological characteristics, chemical constituents, and pharmacological activities.
基金Xinjiang Uygur Autonomous Region Science and Technology Department-Tianshan Innovation Team Program Project,Grant/Award Number:2023D14006Xinjiang Uygur Autonomous Region Science and Technology Department-Natural Science Foundation,Grant/Award Number:2023D01C115。
文摘Background:This study aimed to discover whether Cistanche tubulosa affects the AKT/CASP3 pathway by regulating m6A methylation,to exert a protective effect against peripheral nerve injury in a Parkinson's Disease(PD)mouse model.Methods:In this study,network pharmacology analysis and the molecular docking virtual screening technique was used to filter Acteoside(Act),a potential neuroprotective agent of active components in Cistanche tubulosa.A PD-related peripheral neuropathy mouse model was established by MPTP induction,followed by 21 days treatment of oral Act(25,50,and 100 mg kg^(−1)).Pole climbing,automatic avoidance ability and hot plate sensory tests were evaluated to determine behavioral changes caused by central and peripheral nerve injury.The pathological alterations of dorsal root ganglion tissue and the protein levels of IL-6,AKT,and CASP3 under Act intervention,as well as the dynamic changes of FTO,METTL3,and YTHDF2 which are closely related to m6A methylation,were comprehensively analyzed to observe the peripheral nerve protective efficacy of Act.Results:The results showed that peripheral neuropathy occurring with PD in the mouse model,which could be verified by behavioral tests and pathological histological changes.In addition to the previously established protective effect of Act on dopaminergic neurons in substantia nigra(SN),extensive follow-up studies demonstrated that Act effectively induced m6A methylation,which could further regulate the AKT/CASP3 pathway to play a therapeutic role.In this study,medium and high doses of Act played more obvious therapeutic roles.Conclusion:These findings suggest that Act may regulate the severity of peripheral nerve injury under the activation of the AKT/CASP3 signaling pathway by balancing the methylation level of m6A.These results provide a theoretical basis and experimental evidence for further research on the protective effect of Cistanche tubulosa on both the central and peripheral nerves in the treatment of PD.
文摘Introduction The plants of genus Cistanche G. Beck, which belong to Orobanchaceae family, include about twenty species, and there are four species and one variety are distributed in the northwest part of China. The dried fleshy stem of Cistanche genus plant known as ＇Roucongrong＇ in Chinese traditional medicine, has long been used for kidney deficiency, female infertility, morbid leucorrhea, and neurasthenia. Studies on active components and pharmacological activities show that it possesses significant activities in enhancing potency, anti-fatigue, immuno-modulability, etc, and phenylethanoid glycosides are the major active components. Due to excessive exploitation, its natural resources are facing with exhaustion. In order to improve this situation, the plant has been cultivated on a large scale in the northwest part of China. Up to now, the chemical investigations of the cultivated Cistartcbe salsa have not been undertaken. In this paper we report seven phenylethanoid glycosides from the cultivated Cistanche salsa： echinacoside （1）, cistanoside A （2）, acteoside （3）, isoacteoside （4）, 2＇-acetylacteoside （5）, tubuloside B （6）, and eutigoside A （7）. Among them, compound 7 isolated fronl family Orobanchaceae was reported previously, and compounds 1, 2, 3, 4, 5 and 6 from the cultivated Cistanche salsa are reported for the first time.
文摘Aim A capillary zone electrophoretic method （CZE） was used to determine the interactions between natural products and thrombin. Methods Samples containing natural products and thrombin at various ratios were incubated at 25 ℃ and then were separated by CZE with Tris-acetate buffer at pH 7.2. Each run could be accomplished within 5 min. Results In CZE, the peak width broadened due to the affinity interaction between natural products and thrombin. Compared with positive and negative control, the natural products （CB-1, CB-2） from Coreopsis tinctoria Nuttt. interacted with thrombin; CB-3 from Coreopsis tinctoria Nuttt. and HC-1, HC-2, HC-3 from Cistanche deserticola Ma. did not bind to thrombin. Both qualification and quantification characterizations of the binding were determined. Conclusion The established method is capable of sensitive and fast determination of natural products and thrombin interactions, it can be employed as an alternative method.
基金Natural Science Foundation of Chongqing,No.cstc2017jcyjAX0306。
文摘BACKGROUND Constipation is one of the chronic gastrointestinal functional diseases.It seriously affects the quality of life.Cistanche deserticola(C.deserticola)can treat constipation obviously,but its mechanism has not been clarified.We supposed that mechanism of it improved the intestinal motility by stimulating interstitial Cajal cells(ICC).Activation of the C-kit receptor on the surface of ICC is closely related to ICC function,and the stem cell factor(SCF)/C-kit signaling pathways plays an important role on it.To investigate the mechanism of how C.deserticola treats constipation,this study aimed to establish a constipation model in rats and explore the role of SCF/C-kit signaling pathway in the treatment.AIM To explore the SCF/C-kit signaling pathways in the role of C.deserticola for treatment of constipation by a constipation rat model.METHODS Forty-eight 8-mo-old Sprague–Dawley rats were divided into 4 groups by random weight method:Normal group(n=12),model group(n=12),C.deserticola group(n=12)and blocker group(n=12).The normal group received normal saline by gavage;the model group received loperamide by gavage;the blocker group received loperamide and C.deserticola by gavage,and STI571 was injected by intraperitoneally.During treatment,the weight,fecal granules and fecal quality were recorded every 10 d.On day 20 after model induction,the colon tissues of each group were removed.Hematoxylin and eosin staining was used to observe pathological changes.Expression levels of SCF,C-kit and Aquaporin genes were detected by immunohistochemistry,western blotting,and real-time-quantitative polymerase chain reaction.The colonic epithelial mitochondria and goblet cells were observed by transmission electron microscopy.RESULTS Compared with the normal group,as treatment progressed,the weight of rats in the model and blocker groups decreased significantly,the stool weight decreased,and the stool quality was dry(P<0.05).C.deserticola reversed the decrease in body weight and stool weight and improved stool quality.Histopathological analysis indicated that the colonic mucosal epithelium in the model group was incomplete,and the arrangement of the glands was irregular or damaged.Treatment with C.deserticola improved the integrity and continuity of the epithelial cells and regular arrangement of the glands.The blocking agents inhibited the effects of C.deserticola Immunohistochemistry and real-timequantitative polymerase chain reaction showed that expression of SCF and C-kit protein or genes in the colonic tissue of the model group was decreased(P<0.05),while treatment with C.deserticola increased protein or gene expression(P<0.05).Western blotting showed that expression of aquaporin APQ3 was increased,while the expression of Cx43 decreased in the model group.Treatment with C.deserticola inhibited expression of APQ3 and promoted expression of Cx43.Transmission electron microscopy showed that the mitochondria of the colonic epithelium in the model group were swollen and arranged disorderly,and microvilli were sparse.The condition was better in the C.deserticola group.Mice treated with STI571 blocker confirmed that blocking the SCF/C-kit pathway inhibited the improvement of constipation by C.deserticola.CONCLUSION C.deserticola improved defecation in rats with constipation,and the SCF/C-kit signaling pathway,which is a key link of ICC function,played an important role of the treatment.
