The fuorescence-based in vivo flow cytometry(IVFC)is an emerging tool to monitor eirculating cells in vivo.As a noninvasive and real-timne diagnostic technology,the fluorescence based IVFC allows long-term monitoring ...The fuorescence-based in vivo flow cytometry(IVFC)is an emerging tool to monitor eirculating cells in vivo.As a noninvasive and real-timne diagnostic technology,the fluorescence based IVFC allows long-term monitoring of circulating cells without changing their native biological environment.It has been applied for various biological applications(eg,monitoring circulating tumor cells).In this work,we will review our recent works on fluorescence-based IVFC.The operation principle and typical biological applications will be introduced.In addition,the recent advances in IVFC flow cytometry based on photoacoustic effects and other label free detection methods such as imaging based methods,difuse-light methods,hybrid multimodality methods and multispectral methods are also summarized.展开更多
In the Sichuan Basin,the drilling of complex deep and ultradeep wells is restricted by the casing sequence.Multiple pressure systems are usually encountered in the same open hole section,and narrow safety density wind...In the Sichuan Basin,the drilling of complex deep and ultradeep wells is restricted by the casing sequence.Multiple pressure systems are usually encountered in the same open hole section,and narrow safety density window is observed in vertical strata.The application of managed pressure drilling(MPD)technique which is often used in well drilling ensures the safe and efficient drilling,but it also brings about great challenges to the small-gap liner cementing at the lower part.Therefore,it is of great practical significance to research a cementing technology that can not only satisfy the quality requirement of small gap liner cementing,but also ensure the safe cementing in the strata with narrow safety density window.In this paper,a cementing technology based on precise managed pressure balancing method was proposed by referring to the successful application of MPD technique.According to the cementing design,the hydrostatic column pressure of annulusfluid is under-balanced(slightly lower than the formation pore pressure),and then a precise managed pressure drilling device is adopted to produce back pressure through wellhead restriction or apply wellhead compensation pressure,so that the balanced pore pressure can be reached in the process of cementing via the wellhead pressure and theflow friction offluid in the annulus.After the cementing is completed,a certain compensation pressure is applied to the annulus continuously to prevent annulus channeling during waiting on cement caused by insufficient static pressure and slurry weight loss.After this technology was applied to an ultradeep exploration well in the Sichuan Basin,the qualification rate of borehole cementing quality reached 97%,and the well sections with good cementing quality accounted for 76%.In conclusion,this technology needs no additional equipment,and only by virtue of the MPD equipment,it can achieve safe cementing construction and cementing quality improvement under high displacement efficiency.展开更多
文摘The fuorescence-based in vivo flow cytometry(IVFC)is an emerging tool to monitor eirculating cells in vivo.As a noninvasive and real-timne diagnostic technology,the fluorescence based IVFC allows long-term monitoring of circulating cells without changing their native biological environment.It has been applied for various biological applications(eg,monitoring circulating tumor cells).In this work,we will review our recent works on fluorescence-based IVFC.The operation principle and typical biological applications will be introduced.In addition,the recent advances in IVFC flow cytometry based on photoacoustic effects and other label free detection methods such as imaging based methods,difuse-light methods,hybrid multimodality methods and multispectral methods are also summarized.
基金supported by the National Major Science and Technology Project"Development demonstration project of large-scale carbonae gas fields in the Sichuan Basin-Research and application of large-scale deep carbonate gas reservoir drilling and production technology"(No:2016ZX05052-003)Pet roChina Major Science and Technology Project"Research and application of key technologies for maintaining 30billion cubic meters of natural gas production in Southwest Oil and Gas Field-Research and application ofdrilling,completion,and oil testing technologies for high temperature and high pressure sulfur-bearing deep wells in the Sichuan Basin"(No:2016E-0608)。
文摘In the Sichuan Basin,the drilling of complex deep and ultradeep wells is restricted by the casing sequence.Multiple pressure systems are usually encountered in the same open hole section,and narrow safety density window is observed in vertical strata.The application of managed pressure drilling(MPD)technique which is often used in well drilling ensures the safe and efficient drilling,but it also brings about great challenges to the small-gap liner cementing at the lower part.Therefore,it is of great practical significance to research a cementing technology that can not only satisfy the quality requirement of small gap liner cementing,but also ensure the safe cementing in the strata with narrow safety density window.In this paper,a cementing technology based on precise managed pressure balancing method was proposed by referring to the successful application of MPD technique.According to the cementing design,the hydrostatic column pressure of annulusfluid is under-balanced(slightly lower than the formation pore pressure),and then a precise managed pressure drilling device is adopted to produce back pressure through wellhead restriction or apply wellhead compensation pressure,so that the balanced pore pressure can be reached in the process of cementing via the wellhead pressure and theflow friction offluid in the annulus.After the cementing is completed,a certain compensation pressure is applied to the annulus continuously to prevent annulus channeling during waiting on cement caused by insufficient static pressure and slurry weight loss.After this technology was applied to an ultradeep exploration well in the Sichuan Basin,the qualification rate of borehole cementing quality reached 97%,and the well sections with good cementing quality accounted for 76%.In conclusion,this technology needs no additional equipment,and only by virtue of the MPD equipment,it can achieve safe cementing construction and cementing quality improvement under high displacement efficiency.
