In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiograph...In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiography(OCTA)suffers from angular-dependent contrast(including blind spots),limited field of view,and prolonged imaging time—challenges that restrict its clinical utility.We introduce a circular interleaving scan OCTA method that overcomes these barriers by enabling 360 deg high-contrast iris angiography with consistent spatiotemporal sampling and optimized motion contrast.The circular scan design enables directionoptimized sampling:we configured circumferential sampling density to approximately twice the radial density,enhancing detection of radially oriented iris vasculature.A Cartesian–polar coordinate transformation was implemented for eye-motion compensation,vessel realignment,and vasculature reconstruction.Compared with raster scan OCTA,our circular scan protocol demonstrates 1.55×higher efficiency in iris vascular imaging,featuring a superior duty cycle(99.95%versus 82.00%)and eliminating redundant data acquisition from rectangular field corners(27.3%of the circular area).This method improves vessel density measurement by 39.0%and vessel count quantification by 25.2%relative to raster scans.By eliminating angular-dependent blind spots,our method significantly enhances vascular quantification reliability,paving the way to a better understanding of ocular diseases and holding promising potential for future clinical applications.展开更多
基金supported by the National Key Research and Development Program of China(Grant No.2021YFF0502900)the National Natural Science Foundation of China(Grant Nos.62575066 and 62027824)+3 种基金the Guangdong Basic and Applied Basic Research Foundation(Grant No.2024A1515011344)the Innovation and Entrepreneurship Teams Project of Guangdong Pearl River Talents Program(Grant No.2019ZT08Y105)the Guangdong-Hong Kong-Macao Intelligent Micro-Nano Optoelectronic Technology Joint Laboratory(Grant No.2020B1212030010)the National Institutes of Health/National Eye Institute(NIH/NEI)(Grant Nos.P30EY07551,R01EY022362,and R01EY022362).
文摘In vivo imaging of human iris vasculature remains a persistent challenge,limiting our understanding of its relationship with ocular disease pathogenesis.Conventional raster scan optical coherence tomography angiography(OCTA)suffers from angular-dependent contrast(including blind spots),limited field of view,and prolonged imaging time—challenges that restrict its clinical utility.We introduce a circular interleaving scan OCTA method that overcomes these barriers by enabling 360 deg high-contrast iris angiography with consistent spatiotemporal sampling and optimized motion contrast.The circular scan design enables directionoptimized sampling:we configured circumferential sampling density to approximately twice the radial density,enhancing detection of radially oriented iris vasculature.A Cartesian–polar coordinate transformation was implemented for eye-motion compensation,vessel realignment,and vasculature reconstruction.Compared with raster scan OCTA,our circular scan protocol demonstrates 1.55×higher efficiency in iris vascular imaging,featuring a superior duty cycle(99.95%versus 82.00%)and eliminating redundant data acquisition from rectangular field corners(27.3%of the circular area).This method improves vessel density measurement by 39.0%and vessel count quantification by 25.2%relative to raster scans.By eliminating angular-dependent blind spots,our method significantly enhances vascular quantification reliability,paving the way to a better understanding of ocular diseases and holding promising potential for future clinical applications.
