Circular aptamers are promising candidates for analytical and therapeutic applications due to their enhanced biological and structural stability.However,the process of circular aptamer selection remains a great challe...Circular aptamers are promising candidates for analytical and therapeutic applications due to their enhanced biological and structural stability.However,the process of circular aptamer selection remains a great challenge,as it requires multiple rounds of binding-separation-amplification that involves issues with nonspecific binding and amplification bias.Here,we develop a highly practical solution for reliable selection of circular aptamers in a single round based on magnetosome-like magnetic chain cross-linked graphene oxide(separation efficiency≈105).High-affinity aptamer candidates can be rapidly selected from a preenriched circular DNA library,while low-affinity candidates are effectively adsorbed and separated by magnetosome-like magnetic chain cross-linked graphene oxide.With lipopolysaccharide as a representative model,the single-round selected lipopolysaccharide circular aptamer has been identified to have a high binding affinity with a Kd value of low to nanomolar range.Using this method,circular aptamers for protein and small-molecule targets were also successfully generated.We envision that this approach will accelerate the discovery of various new circular aptamers and open up a new avenue for analytical and therapeutic studies.展开更多
基金supported by the National Natural Science Foundation of China(22104028,32072306,and U23A20265).
文摘Circular aptamers are promising candidates for analytical and therapeutic applications due to their enhanced biological and structural stability.However,the process of circular aptamer selection remains a great challenge,as it requires multiple rounds of binding-separation-amplification that involves issues with nonspecific binding and amplification bias.Here,we develop a highly practical solution for reliable selection of circular aptamers in a single round based on magnetosome-like magnetic chain cross-linked graphene oxide(separation efficiency≈105).High-affinity aptamer candidates can be rapidly selected from a preenriched circular DNA library,while low-affinity candidates are effectively adsorbed and separated by magnetosome-like magnetic chain cross-linked graphene oxide.With lipopolysaccharide as a representative model,the single-round selected lipopolysaccharide circular aptamer has been identified to have a high binding affinity with a Kd value of low to nanomolar range.Using this method,circular aptamers for protein and small-molecule targets were also successfully generated.We envision that this approach will accelerate the discovery of various new circular aptamers and open up a new avenue for analytical and therapeutic studies.
