Pseudounipolar neurons in the dorsal root ganglia(DRG),as the central nodes of primary sensory afferents,possess a distinctive T-junction that is not merely a morphological peculiarity but also performs complex roles ...Pseudounipolar neurons in the dorsal root ganglia(DRG),as the central nodes of primary sensory afferents,possess a distinctive T-junction that is not merely a morphological peculiarity but also performs complex roles in rapid,multiplexed shunting and regulation of sensory signals.This specialized geometry enables separation,filtering,and feedback regulation of neuronal signals,thereby coordinating peripheral and central responses at multiple levels.Recent advances,including spatial transcriptomics,single-cell sequencing,super-resolution microscopy,organoid models,and novel electrophysiological methods,have permitted more precise dissection of the T-junction's molecular composition,ion-channel distribution,and electrophysiological properties.Here,we review current knowledge of the T-junction's developmental regulation and multilayered molecular networks,and we detail its functional alterations in both physiological signaling and pathological pain states,with particular emphasis on ion-channel modulation,signal attenuation,and selective transmission mechanisms.Finally,we discuss contemporary pain-intervention approaches and prospects for precision-targeted therapies,aiming to provide a theoretical foundation for future studies in pain physiology and clinical translation.展开更多
This study investigates the droplet formation for the liquid–liquid two-phase flow within a square T-junction microchannel through numerical simulation using volume of fluid method and experimental visualization usin...This study investigates the droplet formation for the liquid–liquid two-phase flow within a square T-junction microchannel through numerical simulation using volume of fluid method and experimental visualization using high-speed camera imaging.The T-junction microchannel has a cross-sectional width of 0.6 mm and a total length of 27.3 mm.The solution of cyclohexane with 2%and 3%mass concentrations of sorbitan trioleate surfactant were used as the continuous phase,and water was used as the dispersed phase.Slug flow,characteristic of squeezing regime,were predominantly observed.The effects of liquid–liquid two-phase flow rate ratio,and dimensionless number on droplet size,and pressure drop were investigated.The squeezing regime was mapped for 0.0005≤Ca_(c)≤0.0052(capillary number)and 0.1≤q≤10(flow rate ratio).The pressure drops of slugs were in the range from 40 Pa to 200 Pa.The slug lengths were measured between 1 mm and 9 mm.A universal flow map dependent on Ca_(c)Re_(d)^(0.5) are projected to investigate the droplet formation behavior in T-junction microchannel.Correlation expressions are proposed to predict pressure drops and the slug lengths for liquid–liquid two-phase flow in a square T-junction microchannel,using dimensionless numbers such as flow rate ratio and capillary number.The result shows that large continuous phase flow rates facilitate smaller slugs,whereas higher dispersed phase flow rates result in longer shorts.展开更多
Droplet-based microfluidics is a transformative technology with applications across diverse scientific and industrial domains.However,predicting the droplet size generated by individual microchannels before experiment...Droplet-based microfluidics is a transformative technology with applications across diverse scientific and industrial domains.However,predicting the droplet size generated by individual microchannels before experiments or simulations remains a significant challenge.In this study,we focus on a double T-junction microfluidic geometry and employ a hybrid modeling approach that combines machine learning with metaheuristic optimization to address this issue.Specifically,particle swarm optimization(PSO)is used to optimize the hyperparameters of a decision tree(DT)model,and its performance is compared with that of a DT optimized through grid search(GS).The hybrid models are developed to estimate the droplet diameter based on four parameters:the main width,side width,thickness,and flow rate ratio.The dataset of more than 300 cases,generated by a three-dimensional numerical model of the double T-junction,is used for training and testing.Multiple evaluation metrics confirm the predictive accuracy of the models.The results demonstrate that the proposed DT-PSO model achieves higher accuracy,with a coefficient of determination of 0.902 on the test data,while simultaneously reducing prediction time.This methodology holds the potential to minimize design iterations and accelerate the integration of microfluidic technology into the biological sciences.展开更多
Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process...Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process involves neurovascular communication that facilitates the transport of mediators among cerebrovascular endothelial cells,pericytes,glial cells,and neurons,thereby transmitting signals from donor to recipient cells to elicit a collaborative response.展开更多
The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subjec...The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.展开更多
Urban areas worldwide face escalating challenges in managing municipal solid waste (MSW) due to rapid urbanization, population growth, and changing consumption patterns. Inefficient waste management systems contribute...Urban areas worldwide face escalating challenges in managing municipal solid waste (MSW) due to rapid urbanization, population growth, and changing consumption patterns. Inefficient waste management systems contribute to environmental degradation, public health risks, and resource depletion, underscoring the need for innovative solutions. This review employing AI-driven sorting technologies in urban waste management as a transformative framework for sustainable MSW management, emphasizing waste reduction, resource recovery, and closed-loop systems. The paper synthesizes existing literature, case studies, and technological advancements to explore strategies for integrating CE principles into MSW management. Key areas of focus include the application of emerging technologies such as artificial intelligence, machine learning, and big data analytics;advancements in waste-to-resource technologies;the development of scalable and adaptable CE models tailored to diverse urban contexts;and fostering collaboration among governments, private sectors, and communities. Findings highlight the potential of CE frameworks to minimize waste generation, enhance resource efficiency, and create resilient urban systems. However, significant barriers remain, including technological, financial, and policy challenges. The review concludes by identifying future research directions and actionable recommendations for stakeholders, aiming to advance the global transition toward sustainable urban waste management.展开更多
Understanding and predicting droplet breakup is essential in droplet-based microfluidic systems,as it enables precise control over droplet manipulation for various applications.In this study,droplet breakup behavior i...Understanding and predicting droplet breakup is essential in droplet-based microfluidic systems,as it enables precise control over droplet manipulation for various applications.In this study,droplet breakup behavior in a T-junction microchannel is investigated under the influence of microchannel geometry using three-dimensional numerical simulations.A theoretical model is developed based on the balance between surface tension and viscous drag forces acting on the droplet,incorporating the effects of geometric parameters on droplet length.This model predicts the critical Capillary number required for breakup to occur.The theoretical predictions are validated using both previous research data and the present numerical simulations.The results show that the model accurately predicts the transition between breakup and non-breakup regimes.Specifically,an increase in sidearm length ratio inhibits droplet breakup and leads to an asymmetric breakup regime.Furthermore,increasing the outlet-to-inlet width ratio also reduces the likelihood of droplet breakup.These findings provide a predictive framework for understanding and controlling droplet dynamics in microfluidic T-junctions,with potential applications in lab-on-a-chip technologies.展开更多
To address the challenges of varied aircraft skin hole detection types and susceptibility to noise,this paper proposes a method based on the perspective of dual contour edge information fusion.The core method combines...To address the challenges of varied aircraft skin hole detection types and susceptibility to noise,this paper proposes a method based on the perspective of dual contour edge information fusion.The core method combines stereoscopic vision and structured light dual contour information consistently,focusing on extracting edge point information around the hole edge to achieve precise detection of circular holes.In this approach,a line multi-directional gradient feature detector (LMGFD) is introduced for locating the holes from plane stereoscopic image.Furthermore,we establish a three-dimensional (3D) circular hole detection method (BPCircle) based on the dual contour edge information fusion.Finally,experiments demonstrate that our proposed method achieves superior accuracy and robustness based on public benchmark dataset and our own collected standard IPCDS dataset (including two-dimensional (2D)images,3D point clouds,and measured data of three-coordinate measuring machine).The dataset and code can be found from https://github.com/Nicholsdqw/123.展开更多
BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognos...BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognostic potential of circRNAs in patients with pneumonia-induced sepsis.METHODS:This prospective cohort study included 40 healthy individuals,60 patients with pneumonia,and 80 patients with pneumonia-induced sepsis.CircRNAs identified through RNA-sequencing were validated using quantitative real-time polymerase chain reaction(qRT-PCR).Spearman correlation analysis was used to evaluate the associations between circRNAs,inflammatory markers,Sequential Organ Failure Assessment(SOFA)scores,and Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ)scores.Receiver operating characteristic(ROC)curves analysis were used to assess the diagnostic performance of circRNAs,while ROC curves and Kaplan-Meier survival analysis were used to evaluate their prognostic value of 28-day mortality.RESULTS:qRT-PCR confirmed the significant upregulation of Circ-CTD-2281E23.2 and downregulation of Circ-0075723 and Circ-0008679 in sepsis patients.Spearman correlation analysis showed that Circ-CTD-2281E23.2 was positively correlated with inflammatory markers and severity scores,whereas Circ-0075723 and Circ-0008679 were negatively correlated with these parameters.The area under the curve(AUC)values for Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 in diagnosing pneumonia-induced sepsis were 0.728,0.706,and 0.793,respectively.The combination of these circRNAs(AUC=0.846)and the combination with other clinical indicators(AUC=0.990)demostrated enhanced AUC values.The AUC values for Circ-CTD-2281E23.2 and Circ-0075723 in predicting 28-day mortality were 0.664 and 0.765,respectively.CONCLUSION:This study suggest the additional diagnostic and prognostic value of circRNAs in pneumonia-induced sepsis.Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 exhibit diagnostic potential,with Circ-CTD-2281E23.2 and Circ-0075723 showing positive prognostic value for 28-day mortality in sepsis patients.展开更多
Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,t...Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,the impact of the inherent properties of active particles on collective motion under confined conditions remains elusive.Here,we use a highly tunable active nematics model to study active systems under confinement,focusing on the effect of the self-driven speed of active particles.We identify three distinct states characterized by unique particle and flow fields within confined active nematic systems,among which circular rotation emerges as a collective motion involving rotational movement in both particle and flow fields.The theoretical phase diagram shows that increasing the self-driven speed of active particles significantly enhances the region of the circular rotation state and improves its stability.Our results provide insights into the formation of high quality vortices in confined active nematic systems.展开更多
Global acoustic simulations are significant in revealing the internal and physical structure of the Earth.However,due to the limited flexibility of grids and the difficulties in handling boundaries,the traditional fin...Global acoustic simulations are significant in revealing the internal and physical structure of the Earth.However,due to the limited flexibility of grids and the difficulties in handling boundaries,the traditional finite-difference method(FDM)is usually less used in global simulations.Nevertheless,these issues can be well resolved by employing a multi-block structured grid to discretize circular regions.In this paper,we propose an O-H grid approach to partition the circular region and utilize the curvilinear grid finite-difference method(CGFDM)to solve the acoustic wave equation within this circular domain.By appropriately stretching the grid,the interconnections between each grid block are sufficiently smooth for stable information exchange.To verify the efficacy of this method,we conducted three numerical experiments,by comparing results with alternative approaches.Our test results demonstrate good agreement between our findings and the reference solutions.Since the proposed algorithm can effectively solve wave propagation problems in circular regions,it can contribute to 2D global simulation,particularly in interpreting the Earth’s interior.展开更多
Sustainable alternatives have grown more prevalent due to an urgent need to address climate change,environmental degradation,and the depletion of non-renewable resources.As an inexhaustible and renewable resource,biom...Sustainable alternatives have grown more prevalent due to an urgent need to address climate change,environmental degradation,and the depletion of non-renewable resources.As an inexhaustible and renewable resource,biomass has become an attractive option for energy production within the framework of a circular economy that emphasizes resource efficiency and environmental sustainability.Various kinds and sources of biomass,from forestry waste and agricultural residue to animal dung and microalgae,are fully explored in this mini review,along with their potential for biofuel production in both developed and developing countries.The processes for thermochemical and biochemical conversion,the sustainability of using biomass,and the socioeconomic advantages,especially for African countries,are highlighted.Key case studies demonstrating the value and potential of biomass waste in promoting sustainable energy transitions worldwide are also discussed in this review.Despite its potential,the use of biomass is restricted due to challenges including low conversion efficiency,high transportation costs,seasonal variability,and insufficient advancements in technology.Nonetheless,biomass offers an innovative approach for developing an environmentally friendly,efficient,and low-carbon economy that promotes sustainable development and energy security.Holistic approaches,such as increased regional cooperation,capacity building,technical innovation,and policy reform,must be implemented to address existing challenges.展开更多
Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the str...Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the strata but also by the anisotropy of the layered rock mass. The interaction between them can lead to critical structural failure, such as rupture, collapse, or significant deformation within the adjacent rock mass, thereby jeopardizing operational safety. However, the coupling law and mechanism between the stress concentration resulting from the cavities and the anisotropy of a layered rock mass remain unclear. In this study, a uniaxial compression test was performed on shale specimens containing a circular hole to investigate the effects of layer inclination and circular holes on the mechanical properties, elastic energy storage, and failure behaviors of these specimens. The failure mechanism of the rock surrounding the hole was analyzed on the basis of the single plane of weakness theory and the Kirsch solution. The test results indicated pronounced anisotropy in the compressive strength, elastic modulus, and elastic strain energy of the specimens, with distinct “V”, “M” and “U”-shaped patterns correlated with varying layer inclination angles. In addition, the combined effect of stress concentration and layer inclination resulted in different failure types, which were classified into four groups according to their failure behavior. Theoretical analysis revealed that failure around circular holes in layered rock is affected by a range of variables, such as layer inclination, layer strength, lateral pressure coefficient, azimuth, and loading stress.展开更多
Odd elasticity introduces active moduli to the antisymmetric components of the elastic tensor,which describe the asymmetric coupling between different deformation modes in a medium and quantify the work extracted duri...Odd elasticity introduces active moduli to the antisymmetric components of the elastic tensor,which describe the asymmetric coupling between different deformation modes in a medium and quantify the work extracted during quasi-static strain cycles.The introduction of active moduli renders the elastic tensor non-Hermitian,breaking the Maxwell-Betti reciprocity and enabling the observation of phenomena that cannot occur in traditional passive media.Here,we develop an analytical dynamic model for odd elastic circular plates to investigate the effects of odd elasticity on motion in rotationally symmetric geometries.We report a novel nonreciprocal rotating wave and explore the effects of different odd elastic moduli on chiral deformation.Nonreciprocal rotating waves represent a distinct dynamic mode,exhibiting unidirectional propagation with amplitude increasing or decreasing exclusively along a specific direction.The amplitude change during motion reveals the system’s non-conservation of energy.展开更多
Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellu...Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellular and molecular pathways through various mechanisms,such as microRNA sponging.Numerous studies have indicated the regulatory roles of circRNAs in the osteogenic differentiation of stem cells(SCs)isolated from different sources.Dental tissue-derived mesenchymal SCs(MSCs)have received considerable attention in artificial bone engineering,in which SCs are used to manufacture functional bone tissues to repair bone defects.Recently,studies have reported the regulatory roles of circRNAs in the osteogenic differentiation of dental-derived MSCs,such as apical papillae,dental pulp,and dental follicle SCs.This review aimed to discuss the findings of studies evaluating the contribution of circRNAs to the osteogenic differentiation of dental-derived MSCs.展开更多
Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, ...Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, with the aim of solving this problem, circularity metrology of a small cylindrical workpiece using a segmenting scanning method is analyzed. The cross-sectional circle of the cylinder is segmented into several equivalent arcs for measurement by a two-dimensional coordinate measuring machine(profilometer). The circularity contour is obtained by stitching together arc contours obtained by data processing of the coordinates. Different segmenting patterns for coordinate scanning are considered. Measurement results are presented for three segmentation patterns, with 8, 10, and 12 equal segments, respectively.These results are evaluated in terms of the matching coefficient between neighboring arc contours on circumferential stitching, the Euclidean distance between neighboring arc contours on radial stitching, and the curvature of the arcs. From these evaluations, it is found that as the number of segments is increased, the matching coefficient increases from 0.14 to 0.50, the Euclidean distance decreases from 32 nm to 26 nm,and the curvature becomes close to the standard value.展开更多
Circular RNAs(circRNAs)are formed by splicing of precursor RNAs and covalently linked at the 5′and 3′ends.Dysregulated circRNAs are closely related to the epithelial-mesenchymal transition(EMT)of gastrointestinal ma...Circular RNAs(circRNAs)are formed by splicing of precursor RNAs and covalently linked at the 5′and 3′ends.Dysregulated circRNAs are closely related to the epithelial-mesenchymal transition(EMT)of gastrointestinal malignancies.CircRNAs,including circRNA_0008717,circGOT1,circ-DOCK5,circVPS33B,circPVT1,circMET,circ-OXCT1,circ_67835,circRTN4,circ_0087502,circFNDC38,circ_PTEN1,circPGPEP1,and circ-E-Cad are involved in the EMT process of gastrointestinal malignancies through a variety of mechanisms,such as regulating EMT-inducing transcription factors,signaling pathways,and tumor microenvironments.Gastrointestinal(GI)malignancies are common malignant tumors worldwide,and the heterogeneity and easy metastasis of gastrointestinal malignancies limit the effectiveness of medical treatments.Therefore,investigating the molecular mechanisms involved in the pathogenesis of gastrointestinal malignancies is essential for clinical treatment.This article summarizes the biological roles and molecular mechanism of circRNAs in EMT of gastrointestinal malignancies,providing a theoretical basis for applying EMT-related circRNAs in targeted therapy.展开更多
Linear mRNA vaccines are constrained by exonuclease susceptibility and instability,leading to compromised antigen expression.Circular RNA(circRNA) lacking canonical 5' and 3' untranslated regions demonstrates ...Linear mRNA vaccines are constrained by exonuclease susceptibility and instability,leading to compromised antigen expression.Circular RNA(circRNA) lacking canonical 5' and 3' untranslated regions demonstrates intrinsic exonuclease resistance.Current circularization strategies face three principal limitations:chemical methods produce non-native 2',5'-phosphodiester bonds;ribozyme-mediated approaches are restricted to RNA fragments shorter than 500 nucleotides;the Anabaena Group I intron system retains immunogenic exon sequences.In contrast,the self-splicing Group I intron ribozyme from Tetrahymena enables precisely controlled circularization through autonomous structural rearrangement,yielding exonfree constructs.Through optimized purification protocols,historical scalability challenges are systematically addressed.This Perspective establishes the mechanistic rationale and therapeutic superiority of this engineered RNA circularization platform.展开更多
基金supported by grant from the National Key Technology Support Program of the Ministry of Science and Technology of China(No.2021ZD0203204)。
文摘Pseudounipolar neurons in the dorsal root ganglia(DRG),as the central nodes of primary sensory afferents,possess a distinctive T-junction that is not merely a morphological peculiarity but also performs complex roles in rapid,multiplexed shunting and regulation of sensory signals.This specialized geometry enables separation,filtering,and feedback regulation of neuronal signals,thereby coordinating peripheral and central responses at multiple levels.Recent advances,including spatial transcriptomics,single-cell sequencing,super-resolution microscopy,organoid models,and novel electrophysiological methods,have permitted more precise dissection of the T-junction's molecular composition,ion-channel distribution,and electrophysiological properties.Here,we review current knowledge of the T-junction's developmental regulation and multilayered molecular networks,and we detail its functional alterations in both physiological signaling and pathological pain states,with particular emphasis on ion-channel modulation,signal attenuation,and selective transmission mechanisms.Finally,we discuss contemporary pain-intervention approaches and prospects for precision-targeted therapies,aiming to provide a theoretical foundation for future studies in pain physiology and clinical translation.
基金supports for this project from the National Natural Science Foundation of China(22378295).
文摘This study investigates the droplet formation for the liquid–liquid two-phase flow within a square T-junction microchannel through numerical simulation using volume of fluid method and experimental visualization using high-speed camera imaging.The T-junction microchannel has a cross-sectional width of 0.6 mm and a total length of 27.3 mm.The solution of cyclohexane with 2%and 3%mass concentrations of sorbitan trioleate surfactant were used as the continuous phase,and water was used as the dispersed phase.Slug flow,characteristic of squeezing regime,were predominantly observed.The effects of liquid–liquid two-phase flow rate ratio,and dimensionless number on droplet size,and pressure drop were investigated.The squeezing regime was mapped for 0.0005≤Ca_(c)≤0.0052(capillary number)and 0.1≤q≤10(flow rate ratio).The pressure drops of slugs were in the range from 40 Pa to 200 Pa.The slug lengths were measured between 1 mm and 9 mm.A universal flow map dependent on Ca_(c)Re_(d)^(0.5) are projected to investigate the droplet formation behavior in T-junction microchannel.Correlation expressions are proposed to predict pressure drops and the slug lengths for liquid–liquid two-phase flow in a square T-junction microchannel,using dimensionless numbers such as flow rate ratio and capillary number.The result shows that large continuous phase flow rates facilitate smaller slugs,whereas higher dispersed phase flow rates result in longer shorts.
文摘Droplet-based microfluidics is a transformative technology with applications across diverse scientific and industrial domains.However,predicting the droplet size generated by individual microchannels before experiments or simulations remains a significant challenge.In this study,we focus on a double T-junction microfluidic geometry and employ a hybrid modeling approach that combines machine learning with metaheuristic optimization to address this issue.Specifically,particle swarm optimization(PSO)is used to optimize the hyperparameters of a decision tree(DT)model,and its performance is compared with that of a DT optimized through grid search(GS).The hybrid models are developed to estimate the droplet diameter based on four parameters:the main width,side width,thickness,and flow rate ratio.The dataset of more than 300 cases,generated by a three-dimensional numerical model of the double T-junction,is used for training and testing.Multiple evaluation metrics confirm the predictive accuracy of the models.The results demonstrate that the proposed DT-PSO model achieves higher accuracy,with a coefficient of determination of 0.902 on the test data,while simultaneously reducing prediction time.This methodology holds the potential to minimize design iterations and accelerate the integration of microfluidic technology into the biological sciences.
基金supported by the National Natural Science Foundation of China,Nos.82171344(to ZY),82471313(to CKT)the Guangdong Basic and Applied Basic Research Foundation,China,Nos.2023B1515120035,2024A1515012035(to CKT)The Science and Technology Projects in Guangzhou Nos.2025A03J4169(to ZY)。
文摘Stroke-induced alterations in cerebral blood flow trigger neurovascular remodeling,as manifested by the blood-brain barrier dysfunction and subs equent neurovascular repair activities such as angiogenesis.This process involves neurovascular communication that facilitates the transport of mediators among cerebrovascular endothelial cells,pericytes,glial cells,and neurons,thereby transmitting signals from donor to recipient cells to elicit a collaborative response.
文摘The development of sustainable sludge management systems requires looking at them with a new vision in which the concepts of SD(Sustainable Development)must integrate those of CE(Circular Economy),both concepts subject to the principles of TD(Thermodynamics),thus allowing the adoption of actions that are all the more effective the more complete the evaluation of the social dimension has been.This involves a new“Way of thinking”which sees the sludge system as the“Locomotive”of the entire wastewater/sludge treatment train and is developed through“Ways of acting”which includes both“Technical”actions to maximize recoveries of useful materials and/or or energy,and“Socio/Institutional”actions to overcome barriers linked to local cultures and traditions,also considering that the specific local context heavily influences the choices capable of satisfying the concepts of CE.It follows the need of issuing realistic and applicable regulations and overcoming social barriers,such as lack of infrastructure and/or qualified personnel,to achieve an effective integration of the concepts of CE with the more general ones of sustainability.
文摘Urban areas worldwide face escalating challenges in managing municipal solid waste (MSW) due to rapid urbanization, population growth, and changing consumption patterns. Inefficient waste management systems contribute to environmental degradation, public health risks, and resource depletion, underscoring the need for innovative solutions. This review employing AI-driven sorting technologies in urban waste management as a transformative framework for sustainable MSW management, emphasizing waste reduction, resource recovery, and closed-loop systems. The paper synthesizes existing literature, case studies, and technological advancements to explore strategies for integrating CE principles into MSW management. Key areas of focus include the application of emerging technologies such as artificial intelligence, machine learning, and big data analytics;advancements in waste-to-resource technologies;the development of scalable and adaptable CE models tailored to diverse urban contexts;and fostering collaboration among governments, private sectors, and communities. Findings highlight the potential of CE frameworks to minimize waste generation, enhance resource efficiency, and create resilient urban systems. However, significant barriers remain, including technological, financial, and policy challenges. The review concludes by identifying future research directions and actionable recommendations for stakeholders, aiming to advance the global transition toward sustainable urban waste management.
基金funded by the Master,Ph D Scholarship Programme of Vingroup Innovation Foundation(VINIF),code VINIF.2023.Th S.118。
文摘Understanding and predicting droplet breakup is essential in droplet-based microfluidic systems,as it enables precise control over droplet manipulation for various applications.In this study,droplet breakup behavior in a T-junction microchannel is investigated under the influence of microchannel geometry using three-dimensional numerical simulations.A theoretical model is developed based on the balance between surface tension and viscous drag forces acting on the droplet,incorporating the effects of geometric parameters on droplet length.This model predicts the critical Capillary number required for breakup to occur.The theoretical predictions are validated using both previous research data and the present numerical simulations.The results show that the model accurately predicts the transition between breakup and non-breakup regimes.Specifically,an increase in sidearm length ratio inhibits droplet breakup and leads to an asymmetric breakup regime.Furthermore,increasing the outlet-to-inlet width ratio also reduces the likelihood of droplet breakup.These findings provide a predictive framework for understanding and controlling droplet dynamics in microfluidic T-junctions,with potential applications in lab-on-a-chip technologies.
基金supported by the National Natural Science Foundation Youth (No.62303330)。
文摘To address the challenges of varied aircraft skin hole detection types and susceptibility to noise,this paper proposes a method based on the perspective of dual contour edge information fusion.The core method combines stereoscopic vision and structured light dual contour information consistently,focusing on extracting edge point information around the hole edge to achieve precise detection of circular holes.In this approach,a line multi-directional gradient feature detector (LMGFD) is introduced for locating the holes from plane stereoscopic image.Furthermore,we establish a three-dimensional (3D) circular hole detection method (BPCircle) based on the dual contour edge information fusion.Finally,experiments demonstrate that our proposed method achieves superior accuracy and robustness based on public benchmark dataset and our own collected standard IPCDS dataset (including two-dimensional (2D)images,3D point clouds,and measured data of three-coordinate measuring machine).The dataset and code can be found from https://github.com/Nicholsdqw/123.
基金supported by grants from the municipal Natural Science Foundation of Shanghai Scientific Committee of China(22ZR1451000 to TL)the peak supporting clinical discipline of Shanghai Health Bureau(2023ZDFC0104 to TL)+3 种基金the key clinical discipline of Shanghai Pudong Health Bureau(PWZxk2022-17 to TL)the clinical peak discipline of Shanghai Pudong Heath Bureau(PWYgf2021-03)the top-notch innovative medical talents of Shanghai Pudong Health Bureau(2025PDWSYCBJ-03 to TL)the leading medical talent project of Shanghai Pudong Heath Bureau(PWR12020-07 to LS)。
文摘BACKGROUND:Circulating biomarkers for sepsis are lacking,and research on circular RNAs(circR NAs)as potential biomarkers of pneumonia-induced sepsis is limited.This study aims to investigate the diagnostic and prognostic potential of circRNAs in patients with pneumonia-induced sepsis.METHODS:This prospective cohort study included 40 healthy individuals,60 patients with pneumonia,and 80 patients with pneumonia-induced sepsis.CircRNAs identified through RNA-sequencing were validated using quantitative real-time polymerase chain reaction(qRT-PCR).Spearman correlation analysis was used to evaluate the associations between circRNAs,inflammatory markers,Sequential Organ Failure Assessment(SOFA)scores,and Acute Physiology and Chronic Health EvaluationⅡ(APACHEⅡ)scores.Receiver operating characteristic(ROC)curves analysis were used to assess the diagnostic performance of circRNAs,while ROC curves and Kaplan-Meier survival analysis were used to evaluate their prognostic value of 28-day mortality.RESULTS:qRT-PCR confirmed the significant upregulation of Circ-CTD-2281E23.2 and downregulation of Circ-0075723 and Circ-0008679 in sepsis patients.Spearman correlation analysis showed that Circ-CTD-2281E23.2 was positively correlated with inflammatory markers and severity scores,whereas Circ-0075723 and Circ-0008679 were negatively correlated with these parameters.The area under the curve(AUC)values for Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 in diagnosing pneumonia-induced sepsis were 0.728,0.706,and 0.793,respectively.The combination of these circRNAs(AUC=0.846)and the combination with other clinical indicators(AUC=0.990)demostrated enhanced AUC values.The AUC values for Circ-CTD-2281E23.2 and Circ-0075723 in predicting 28-day mortality were 0.664 and 0.765,respectively.CONCLUSION:This study suggest the additional diagnostic and prognostic value of circRNAs in pneumonia-induced sepsis.Circ-CTD-2281E23.2,Circ-0075723,and Circ-0008679 exhibit diagnostic potential,with Circ-CTD-2281E23.2 and Circ-0075723 showing positive prognostic value for 28-day mortality in sepsis patients.
基金supported by the National Key Research and Development Program of China under Grant No.2022YFA1405000Innovation Program for Quantum Science and Technology under Grant No.2024ZD0300101the National Natural Science Foundation of China under Grant Nos.12274212,12174184,12347102。
文摘Active matter exhibits collective motions at various scales.Geometric confinement has been identified as an effective way to control and manipulate active fluids,with much attention given to external factors.However,the impact of the inherent properties of active particles on collective motion under confined conditions remains elusive.Here,we use a highly tunable active nematics model to study active systems under confinement,focusing on the effect of the self-driven speed of active particles.We identify three distinct states characterized by unique particle and flow fields within confined active nematic systems,among which circular rotation emerges as a collective motion involving rotational movement in both particle and flow fields.The theoretical phase diagram shows that increasing the self-driven speed of active particles significantly enhances the region of the circular rotation state and improves its stability.Our results provide insights into the formation of high quality vortices in confined active nematic systems.
基金supported by National Key Research and Development Program of China(No.2022YFF0800602)Guangdong Provincial Key Laboratory of Geophysical High-resolution Imaging Technology(No.2022B1212010002)Shenzhen Science and Technology Program(No.KQTD20170810111725321).
文摘Global acoustic simulations are significant in revealing the internal and physical structure of the Earth.However,due to the limited flexibility of grids and the difficulties in handling boundaries,the traditional finite-difference method(FDM)is usually less used in global simulations.Nevertheless,these issues can be well resolved by employing a multi-block structured grid to discretize circular regions.In this paper,we propose an O-H grid approach to partition the circular region and utilize the curvilinear grid finite-difference method(CGFDM)to solve the acoustic wave equation within this circular domain.By appropriately stretching the grid,the interconnections between each grid block are sufficiently smooth for stable information exchange.To verify the efficacy of this method,we conducted three numerical experiments,by comparing results with alternative approaches.Our test results demonstrate good agreement between our findings and the reference solutions.Since the proposed algorithm can effectively solve wave propagation problems in circular regions,it can contribute to 2D global simulation,particularly in interpreting the Earth’s interior.
文摘Sustainable alternatives have grown more prevalent due to an urgent need to address climate change,environmental degradation,and the depletion of non-renewable resources.As an inexhaustible and renewable resource,biomass has become an attractive option for energy production within the framework of a circular economy that emphasizes resource efficiency and environmental sustainability.Various kinds and sources of biomass,from forestry waste and agricultural residue to animal dung and microalgae,are fully explored in this mini review,along with their potential for biofuel production in both developed and developing countries.The processes for thermochemical and biochemical conversion,the sustainability of using biomass,and the socioeconomic advantages,especially for African countries,are highlighted.Key case studies demonstrating the value and potential of biomass waste in promoting sustainable energy transitions worldwide are also discussed in this review.Despite its potential,the use of biomass is restricted due to challenges including low conversion efficiency,high transportation costs,seasonal variability,and insufficient advancements in technology.Nonetheless,biomass offers an innovative approach for developing an environmentally friendly,efficient,and low-carbon economy that promotes sustainable development and energy security.Holistic approaches,such as increased regional cooperation,capacity building,technical innovation,and policy reform,must be implemented to address existing challenges.
基金supported by Beijing Natural Science Foundation of China(Grant No.2244099)the China Postdoctoral Science Foundation(Grant No.2023T0025)the National Natural Science Foundation of China(Grant No.52074020).
文摘Layered rock formations are frequently encountered during the excavation of underground structures. The stability of such structures is influenced not only by the stress concentration caused by the cavities in the strata but also by the anisotropy of the layered rock mass. The interaction between them can lead to critical structural failure, such as rupture, collapse, or significant deformation within the adjacent rock mass, thereby jeopardizing operational safety. However, the coupling law and mechanism between the stress concentration resulting from the cavities and the anisotropy of a layered rock mass remain unclear. In this study, a uniaxial compression test was performed on shale specimens containing a circular hole to investigate the effects of layer inclination and circular holes on the mechanical properties, elastic energy storage, and failure behaviors of these specimens. The failure mechanism of the rock surrounding the hole was analyzed on the basis of the single plane of weakness theory and the Kirsch solution. The test results indicated pronounced anisotropy in the compressive strength, elastic modulus, and elastic strain energy of the specimens, with distinct “V”, “M” and “U”-shaped patterns correlated with varying layer inclination angles. In addition, the combined effect of stress concentration and layer inclination resulted in different failure types, which were classified into four groups according to their failure behavior. Theoretical analysis revealed that failure around circular holes in layered rock is affected by a range of variables, such as layer inclination, layer strength, lateral pressure coefficient, azimuth, and loading stress.
基金The Innovative Projects of Key Disciplines of Civil Engineering of Changsha University of Science and Technology,24ZDXK07,Andi Lai。
文摘Odd elasticity introduces active moduli to the antisymmetric components of the elastic tensor,which describe the asymmetric coupling between different deformation modes in a medium and quantify the work extracted during quasi-static strain cycles.The introduction of active moduli renders the elastic tensor non-Hermitian,breaking the Maxwell-Betti reciprocity and enabling the observation of phenomena that cannot occur in traditional passive media.Here,we develop an analytical dynamic model for odd elastic circular plates to investigate the effects of odd elasticity on motion in rotationally symmetric geometries.We report a novel nonreciprocal rotating wave and explore the effects of different odd elastic moduli on chiral deformation.Nonreciprocal rotating waves represent a distinct dynamic mode,exhibiting unidirectional propagation with amplitude increasing or decreasing exclusively along a specific direction.The amplitude change during motion reveals the system’s non-conservation of energy.
文摘Circular RNAs(circRNAs)are a distinct type of nonlinear and noncoding RNAs endogenously expressed by pre-mRNA back-splicing and crucial in transcriptional and posttranscriptional regulation.CircRNAs can regulate cellular and molecular pathways through various mechanisms,such as microRNA sponging.Numerous studies have indicated the regulatory roles of circRNAs in the osteogenic differentiation of stem cells(SCs)isolated from different sources.Dental tissue-derived mesenchymal SCs(MSCs)have received considerable attention in artificial bone engineering,in which SCs are used to manufacture functional bone tissues to repair bone defects.Recently,studies have reported the regulatory roles of circRNAs in the osteogenic differentiation of dental-derived MSCs,such as apical papillae,dental pulp,and dental follicle SCs.This review aimed to discuss the findings of studies evaluating the contribution of circRNAs to the osteogenic differentiation of dental-derived MSCs.
基金supported by the National Defense Basic Scientific Research Program of China(Grant No.JCKY2019427D002).
文摘Owing to eccentricity and inclination, circularity of a cylindrical workpiece cannot be measured precisely by a circularity measuring machine when the workpiece has a small dimension(diameter ≤ 3 mm). In this paper, with the aim of solving this problem, circularity metrology of a small cylindrical workpiece using a segmenting scanning method is analyzed. The cross-sectional circle of the cylinder is segmented into several equivalent arcs for measurement by a two-dimensional coordinate measuring machine(profilometer). The circularity contour is obtained by stitching together arc contours obtained by data processing of the coordinates. Different segmenting patterns for coordinate scanning are considered. Measurement results are presented for three segmentation patterns, with 8, 10, and 12 equal segments, respectively.These results are evaluated in terms of the matching coefficient between neighboring arc contours on circumferential stitching, the Euclidean distance between neighboring arc contours on radial stitching, and the curvature of the arcs. From these evaluations, it is found that as the number of segments is increased, the matching coefficient increases from 0.14 to 0.50, the Euclidean distance decreases from 32 nm to 26 nm,and the curvature becomes close to the standard value.
基金supported by grants from the Key Scientific and Technological Projects of Ningbo(No.2021Z133)Ningbo Top Medical and Health Research Program(No.2023020612)+2 种基金National Natural Science Foundation of China(No.81702367)the Medical and Health Research Project of Zhejiang Province(No.2024KY319)the Youth Medical Backbone Talents Training Program of Ningbo.
文摘Circular RNAs(circRNAs)are formed by splicing of precursor RNAs and covalently linked at the 5′and 3′ends.Dysregulated circRNAs are closely related to the epithelial-mesenchymal transition(EMT)of gastrointestinal malignancies.CircRNAs,including circRNA_0008717,circGOT1,circ-DOCK5,circVPS33B,circPVT1,circMET,circ-OXCT1,circ_67835,circRTN4,circ_0087502,circFNDC38,circ_PTEN1,circPGPEP1,and circ-E-Cad are involved in the EMT process of gastrointestinal malignancies through a variety of mechanisms,such as regulating EMT-inducing transcription factors,signaling pathways,and tumor microenvironments.Gastrointestinal(GI)malignancies are common malignant tumors worldwide,and the heterogeneity and easy metastasis of gastrointestinal malignancies limit the effectiveness of medical treatments.Therefore,investigating the molecular mechanisms involved in the pathogenesis of gastrointestinal malignancies is essential for clinical treatment.This article summarizes the biological roles and molecular mechanism of circRNAs in EMT of gastrointestinal malignancies,providing a theoretical basis for applying EMT-related circRNAs in targeted therapy.
基金supported by the National Key Research&Development Program of China(Nos.2021YFC2302400,2021YFA1201000,2023YFC2606004)the Fundamental Research Funds for the Central Universities(No.2022CX01013)。
文摘Linear mRNA vaccines are constrained by exonuclease susceptibility and instability,leading to compromised antigen expression.Circular RNA(circRNA) lacking canonical 5' and 3' untranslated regions demonstrates intrinsic exonuclease resistance.Current circularization strategies face three principal limitations:chemical methods produce non-native 2',5'-phosphodiester bonds;ribozyme-mediated approaches are restricted to RNA fragments shorter than 500 nucleotides;the Anabaena Group I intron system retains immunogenic exon sequences.In contrast,the self-splicing Group I intron ribozyme from Tetrahymena enables precisely controlled circularization through autonomous structural rearrangement,yielding exonfree constructs.Through optimized purification protocols,historical scalability challenges are systematically addressed.This Perspective establishes the mechanistic rationale and therapeutic superiority of this engineered RNA circularization platform.
