A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CHECl2 was effectively degraded by Bacillus circulans WZ-12 ...A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CHECl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g.L^-1 to 10 g-L^-1, and the maximum degradation efficiency （85%） was achieved at NaCl concentration of 10 g.L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH2BrCl, C2H4Cl2, and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine （GB）, but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation （R2= 0.85-0.94） was observed between the rate of biodegradation （V） and salt concentration from 5 g.L〈 to 60 g.L-1. The growing cells （in minimal salt medium） degraded approximately 50% of the CH2C12 within 24 h, whereas the resting cells （in physiological saline） degraded only 25% of the CH2C12 within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industriai efflu- ents that contain chlorinated hydrocarbons with high salt concentrations.展开更多
Phosphate-solubilizing bacterial strains(6 Nos.) were isolated from the rhizosphere soils of two seagrasses(Halophila ovalis(R.Br.) Hook and Halodule pinifolia(Miki) Hartog) in the Vellar estuary.Experimental studies ...Phosphate-solubilizing bacterial strains(6 Nos.) were isolated from the rhizosphere soils of two seagrasses(Halophila ovalis(R.Br.) Hook and Halodule pinifolia(Miki) Hartog) in the Vellar estuary.Experimental studies found that the strain PSSG6 was effective in phosphate solubilization with Phosphate Solubilization efficiency index E = 375 ± 8.54,followed by the strain PSSG5 with Phosphate Solubilization efficiency index E = 275 ± 27.3.Of the 6 strains isolated,the strains PSSG4 and PSSG5 be-longed to the genus Bacillus,and PSSG1,PSSG2 and PSSG3 were identified as Citrobacter sp.,Shigella sp.,and Klebsiella sp.,respectively,by conventional method,and PSSG6 was identified as Bacillus circulans using conventional and molecular methods.展开更多
Objective:To evaluate the effects of four different concentrations of Bacillus subtilis(B.subtilis)and Bacillus circulance(B.circulance)(1×10^(4),2×10^(4),3×10^(4)and 4×10^(4)CFU/g)on growth and re...Objective:To evaluate the effects of four different concentrations of Bacillus subtilis(B.subtilis)and Bacillus circulance(B.circulance)(1×10^(4),2×10^(4),3×10^(4)and 4×10^(4)CFU/g)on growth and resistance of three spot gourami(Trichopodus trichopterus)for a period of 30 days.Methods:During this study,experimental fish were fed with supplemented diets.Bacillus probiotics with concentrations of 1×10^(4)to 4×10^(4)CFU/g were administered to improve the growth performance and larval resistance to challenge tests containing acidic pH and basic pH,ammonia and salinity tests.Results:The addition of B.subtilis and B.circulance to the diet did not increase larval growth rate,but increased larval resistance against the challenge tests.Conclusions:The results of this study investigated the positive effects of B.subtilis and B.circulance on Trichopodus trichopterus resistance time to the challenge test.展开更多
基金Supported by the National Natural Science Foundation of China （20977087, 20976165）, Zhejiang Provincial Key Science and Technology Project of China （2011C13023）, and Zhejiang Provincial Natural Science Foundation of China （Y5090155, Y5090054）.
文摘A novel saline-tolerant bacterium Bacillus circulans WZ-12 was evaluated for its potential to degrade four chlorinated hydrocarbons under saline conditions. CHECl2 was effectively degraded by Bacillus circulans WZ-12 cells in the medium containing NaCl concentrations ranging from 5 g.L^-1 to 10 g-L^-1, and the maximum degradation efficiency （85%） was achieved at NaCl concentration of 10 g.L^-1. Similarly, Bacillus circulans WZ-12 was able to degrade CH2BrCl, C2H4Cl2, and C2H2Cl2 in the presence of 10 g NaCl per liter within 24 h. Cells of Bacillus circulans WZ-12 grown in minimal salt medium contained low levels of glycine betaine （GB）, but GB levels were 3- to 5-fold higher in cells grown in media with high salt. Kinetic analysis revealed that biodegradation of the four chlorinated hydrocarbons was concentration dependent and a linear inverse correlation （R2= 0.85-0.94） was observed between the rate of biodegradation （V） and salt concentration from 5 g.L〈 to 60 g.L-1. The growing cells （in minimal salt medium） degraded approximately 50% of the CH2C12 within 24 h, whereas the resting cells （in physiological saline） degraded only 25% of the CH2C12 within 24 h and were inactive after 36 h cultivation. Biodegradation could be repeatedly performed for more than 192 h with more than 50% removal efficiency. Bacillus circulans WZ-12 grows well in an aqueous/oil system, hence, it is effective for the treatment of industriai efflu- ents that contain chlorinated hydrocarbons with high salt concentrations.
文摘Phosphate-solubilizing bacterial strains(6 Nos.) were isolated from the rhizosphere soils of two seagrasses(Halophila ovalis(R.Br.) Hook and Halodule pinifolia(Miki) Hartog) in the Vellar estuary.Experimental studies found that the strain PSSG6 was effective in phosphate solubilization with Phosphate Solubilization efficiency index E = 375 ± 8.54,followed by the strain PSSG5 with Phosphate Solubilization efficiency index E = 275 ± 27.3.Of the 6 strains isolated,the strains PSSG4 and PSSG5 be-longed to the genus Bacillus,and PSSG1,PSSG2 and PSSG3 were identified as Citrobacter sp.,Shigella sp.,and Klebsiella sp.,respectively,by conventional method,and PSSG6 was identified as Bacillus circulans using conventional and molecular methods.
文摘Objective:To evaluate the effects of four different concentrations of Bacillus subtilis(B.subtilis)and Bacillus circulance(B.circulance)(1×10^(4),2×10^(4),3×10^(4)and 4×10^(4)CFU/g)on growth and resistance of three spot gourami(Trichopodus trichopterus)for a period of 30 days.Methods:During this study,experimental fish were fed with supplemented diets.Bacillus probiotics with concentrations of 1×10^(4)to 4×10^(4)CFU/g were administered to improve the growth performance and larval resistance to challenge tests containing acidic pH and basic pH,ammonia and salinity tests.Results:The addition of B.subtilis and B.circulance to the diet did not increase larval growth rate,but increased larval resistance against the challenge tests.Conclusions:The results of this study investigated the positive effects of B.subtilis and B.circulance on Trichopodus trichopterus resistance time to the challenge test.
