BACKGROUND Circular RNAs(circRNAs)are critical regulators in tumorigenesis,functioning as microRNA sponges or protein decoys.Although numerous circRNAs have been implicated in gastric cancer progression,the role of hs...BACKGROUND Circular RNAs(circRNAs)are critical regulators in tumorigenesis,functioning as microRNA sponges or protein decoys.Although numerous circRNAs have been implicated in gastric cancer progression,the role of hsa_circRNA_101996 remains unclear.This study hypothesizes that hsa_circRNA_101996 promotes gastric cancer cell proliferation and apoptosis via the microRNA-577(miR-577)/high mobility group nucleosome binding domain 5(HMGN5)axis.AIM To investigate the role of hsa_circRNA_101996 in gastric cancer proliferation and apoptosis through the miR-577/HMGN5 axis.METHODS Forty-one paired gastric cancer tissues and adjacent non-cancerous tissues were analyzed.Differential circRNA expression was identified using GSE83521 and GSE89143 datasets.miR-577 and HMGN5 were predicted via CircInteractome and TargetScan.Functional experiments(MTT,colony formation,Western blot)and dual-luciferase reporter assays were performed in gastric cancer cell lines(OCUM-1,HSC-39).In vivo tumorigenesis was validated in nude mice.Statistical analysis included Student’s t-test and one-way ANOVA(P<0.05).RESULTS Hsa_circRNA_101996 was significantly upregulated in gastric cancer tissues and cell lines compared to adjacent non-cancerous tissues(P<0.05).Dual-luciferase reporter assays validated the interactions among hsa_circRNA_101996,miR-577,and HMGN5.In vitro,gastric cancer cells overexpressing hsa_circRNA_101996 showed significantly increased proliferation and decreased apoptosis compared to controls(P<0.05).Cells transfected with miR-577 mimics exhibited reduced proliferation and increased apoptosis(P<0.05).Co-transfection with hsa_circRNA_101996 or HMGN5 reversed the effects of miR-577 mimics.In vivo,hsa_circRNA_101996-overexpressing tumors showed increased volume and HMGN5 expression(P<0.05).CONCLUSION Hsa_circRNA_101996 promotes gastric cancer progression by sponging miR-577 to upregulate HMGN5,suggesting a novel therapeutic target for gastric cancer.展开更多
目的:探讨circRNA_0016418及circRNA_0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。方法:选取2018年01月至2021年10月我院收治的68例恶性黑色素瘤患者,将其分为淋巴结转移组26例和无淋巴结转移组42例。采用实时荧光定量...目的:探讨circRNA_0016418及circRNA_0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。方法:选取2018年01月至2021年10月我院收治的68例恶性黑色素瘤患者,将其分为淋巴结转移组26例和无淋巴结转移组42例。采用实时荧光定量PCR法检测恶性黑色素瘤组织及正常组织中circRNA_0016418及circRNA_0017247的水平。应用受试者工作特征(receiver operating characteristic, ROC)曲线分析circRNA_0016418及circRNA_0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。Pearson相关分析检测恶性黑色素瘤患者中circRNA_0016418水平与circRNA_0017247水平的相关性。结果:恶性黑色素瘤组织中circRNA_0016418水平(2.64±1.17 vs 0.95±0.36)及circRNA_0017247水平(5.18±2.50 vs 1.52±0.63)均明显高于正常组织(P<0.001)。淋巴结转移组circRNA_0016418水平(3.85±1.72 vs 1.66±0.74)及circRNA_0017247水平(7.16±3.82 vs 3.38±1.54)均明显高于无淋巴结转移组(P<0.001)。ROC曲线显示,circRNA_0016418及circRNA_0017247二者联合诊断恶性黑色素瘤的曲线下面积为0.905(95%CI:0.847~0.964),其灵敏度为92.8%,特异度为83.5%;二者联合预测淋巴结转移的曲线下面积为0.957(95%CI:0.895~0.993),其灵敏度为98.0%,特异度为84.6%。相关分析显示,恶性黑色素瘤患者circRNA_0016418水平与circRNA_0017247水平呈正相关(r=0.802,P<0.001)。结论:恶性黑色素瘤组织中circRNA_0016418及circRNA_0017247水平明显升高,与淋巴结转移有关,二者联合检测对恶性黑色素瘤诊断及其预测淋巴结转移有很好的价值。展开更多
文摘BACKGROUND Circular RNAs(circRNAs)are critical regulators in tumorigenesis,functioning as microRNA sponges or protein decoys.Although numerous circRNAs have been implicated in gastric cancer progression,the role of hsa_circRNA_101996 remains unclear.This study hypothesizes that hsa_circRNA_101996 promotes gastric cancer cell proliferation and apoptosis via the microRNA-577(miR-577)/high mobility group nucleosome binding domain 5(HMGN5)axis.AIM To investigate the role of hsa_circRNA_101996 in gastric cancer proliferation and apoptosis through the miR-577/HMGN5 axis.METHODS Forty-one paired gastric cancer tissues and adjacent non-cancerous tissues were analyzed.Differential circRNA expression was identified using GSE83521 and GSE89143 datasets.miR-577 and HMGN5 were predicted via CircInteractome and TargetScan.Functional experiments(MTT,colony formation,Western blot)and dual-luciferase reporter assays were performed in gastric cancer cell lines(OCUM-1,HSC-39).In vivo tumorigenesis was validated in nude mice.Statistical analysis included Student’s t-test and one-way ANOVA(P<0.05).RESULTS Hsa_circRNA_101996 was significantly upregulated in gastric cancer tissues and cell lines compared to adjacent non-cancerous tissues(P<0.05).Dual-luciferase reporter assays validated the interactions among hsa_circRNA_101996,miR-577,and HMGN5.In vitro,gastric cancer cells overexpressing hsa_circRNA_101996 showed significantly increased proliferation and decreased apoptosis compared to controls(P<0.05).Cells transfected with miR-577 mimics exhibited reduced proliferation and increased apoptosis(P<0.05).Co-transfection with hsa_circRNA_101996 or HMGN5 reversed the effects of miR-577 mimics.In vivo,hsa_circRNA_101996-overexpressing tumors showed increased volume and HMGN5 expression(P<0.05).CONCLUSION Hsa_circRNA_101996 promotes gastric cancer progression by sponging miR-577 to upregulate HMGN5,suggesting a novel therapeutic target for gastric cancer.
文摘目的:探讨circRNA_0016418及circRNA_0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。方法:选取2018年01月至2021年10月我院收治的68例恶性黑色素瘤患者,将其分为淋巴结转移组26例和无淋巴结转移组42例。采用实时荧光定量PCR法检测恶性黑色素瘤组织及正常组织中circRNA_0016418及circRNA_0017247的水平。应用受试者工作特征(receiver operating characteristic, ROC)曲线分析circRNA_0016418及circRNA_0017247异常表达对恶性黑色素瘤诊断及其预测淋巴结转移的价值。Pearson相关分析检测恶性黑色素瘤患者中circRNA_0016418水平与circRNA_0017247水平的相关性。结果:恶性黑色素瘤组织中circRNA_0016418水平(2.64±1.17 vs 0.95±0.36)及circRNA_0017247水平(5.18±2.50 vs 1.52±0.63)均明显高于正常组织(P<0.001)。淋巴结转移组circRNA_0016418水平(3.85±1.72 vs 1.66±0.74)及circRNA_0017247水平(7.16±3.82 vs 3.38±1.54)均明显高于无淋巴结转移组(P<0.001)。ROC曲线显示,circRNA_0016418及circRNA_0017247二者联合诊断恶性黑色素瘤的曲线下面积为0.905(95%CI:0.847~0.964),其灵敏度为92.8%,特异度为83.5%;二者联合预测淋巴结转移的曲线下面积为0.957(95%CI:0.895~0.993),其灵敏度为98.0%,特异度为84.6%。相关分析显示,恶性黑色素瘤患者circRNA_0016418水平与circRNA_0017247水平呈正相关(r=0.802,P<0.001)。结论:恶性黑色素瘤组织中circRNA_0016418及circRNA_0017247水平明显升高,与淋巴结转移有关,二者联合检测对恶性黑色素瘤诊断及其预测淋巴结转移有很好的价值。
