Echinacea purpurea (Purple coneflower) is an immunostimulating drug, containing multiple substances. The most important substance in activity is polysaccharide, caffeic acid derivatives (cichoric acid), alkamides and ...Echinacea purpurea (Purple coneflower) is an immunostimulating drug, containing multiple substances. The most important substance in activity is polysaccharide, caffeic acid derivatives (cichoric acid), alkamides and glycoproteins. It is not clear yet, which substances are responsible for activity. Cichoric acid is an appropriate marker of the quality of Echinacea purpurea containing product, because it has immune stimulatory effects and it is susceptible to degradation. In this study a TLC scanner system and HPLC method has been used for identification and determination of cichoric acid in aerial parts of Echinacea purpurea. The results showed that the cichoric acid content of Echinacea purpurea cultivated in Iran is about 1.50 ±0.65% (w/w) which is comparable with cichoric acid content in native plants. The local conditions have no significant effect on cichoric acid content as a biomarker of Echinacea purpurea quality.展开更多
The inside of the cell is closely filled with diverse biological macromolecules,which will affect various physi-ological activities in vivo.It is known that natural products have beneficial effects against the formati...The inside of the cell is closely filled with diverse biological macromolecules,which will affect various physi-ological activities in vivo.It is known that natural products have beneficial effects against the formation of advanced glycation end products(AGEs),but their inhibitory actions in macromolecular crowding environment are still poorly understood.Therefore,to investigate the influence of macromolecular crowding environment,the inhibitory effect of cichoric acid(CA)on the non-enzymatic glycation of bovine serum albumin(BSA)in the absence and presence of crowding reagents(PEG 2000 and PEG 4000)was studied.The results demonstrated that CA could significantly inhibit the formation of AGEs than aminoguanidine in the BSA-fructose model with or without PEG,and the inhibition effect in PBS solution was better than that in macromolecular crowding envi-ronment.Moreover,CA presented strong antioxidant capacity and trapping ability of glyoxal,and could stabilize the native structure of BSA caused by the glycation modification,which might be the primary mechanisms for its anti-glycation effect.Additionally,CA tended to bind to site I of BSA mainly through hydrogen bonds and hy-drophobic interactions.These findings indicated that CA could be regarded as a high potential anti-glycation inhibitor to prevent the development of diabetic complications.展开更多
Objective:To establish HPLC fingerprints of different parts of chicory stems,leaves,roots,flowers and seeds,and compare the similarities and differences of chemical components in different parts,so as to provide a sci...Objective:To establish HPLC fingerprints of different parts of chicory stems,leaves,roots,flowers and seeds,and compare the similarities and differences of chemical components in different parts,so as to provide a scientific basis for the comprehensive utilization of chicory.Methods:To establish the HPLC fingerprint of chicory,the chromatographic column was chosen with Agilent ZORBAX Eclipse XDB-C_(18),the mobile phase was methanol(A)-0.2%formic acid(B),the flow rate was 1 mL/min,the column temperature was 30℃,and the detection wavelength was 254 nm.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was used to evaluate the similarity of different parts of decoction pieces,and the determination method of multi-component content was established based on fingerprint identification chromatographic peaks,and the determination results were analyzed.Results:The HPLC fingerprinting method of chicory was established.Sixteen chromatographic peaks were identified and 10 of them were identified as:caftaric acid(1),esculin(2),chlorogenic acid(3),esculetin(4),caffeic acid(5),cichoric acid(8),hyperoside(11),rutin(12),isochlorogenic acid C(14)and luteolin(16).The similarity of different parts was 0.084–0.701.At the same time,the total content of detected chemical components was ranked as flower>leaf>stem>root>seed.Roots did not contain caftaric acid,rutin,and luteolin,flowers did not contain luteolin,and seeds did not contain caftaric acid,cichoric acid,and luteolin.The content of cichoric acid in leaves was the most,and esculin in flowers was the most.Conclusion:The results of HPLC fingerprint and multi-component content determination revealed the similarity and difference of different parts of chicory from chemical composition,indicating that there were certain differences in different parts of chicory.The established HPLC fingerprinting method can provide a reference for quality control and evaluation of different parts of the chicory.展开更多
文摘Echinacea purpurea (Purple coneflower) is an immunostimulating drug, containing multiple substances. The most important substance in activity is polysaccharide, caffeic acid derivatives (cichoric acid), alkamides and glycoproteins. It is not clear yet, which substances are responsible for activity. Cichoric acid is an appropriate marker of the quality of Echinacea purpurea containing product, because it has immune stimulatory effects and it is susceptible to degradation. In this study a TLC scanner system and HPLC method has been used for identification and determination of cichoric acid in aerial parts of Echinacea purpurea. The results showed that the cichoric acid content of Echinacea purpurea cultivated in Iran is about 1.50 ±0.65% (w/w) which is comparable with cichoric acid content in native plants. The local conditions have no significant effect on cichoric acid content as a biomarker of Echinacea purpurea quality.
基金supported by the National Natural Science Foundation of China(21506152)the Natural Science Foundation of Tianjin,China(19JCYBJC21000)+2 种基金the Open Fund of Key Laboratory of Biotechnology and Bioresources Utilization(Dalian Minzu University),Ministry of Education(KF2023006)China,the Foundation of Key Laboratory of Tropical Medicinal Resource Chemistry of Ministry of Education,China(RDZH2021005)State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources,Guangxi Normal University(CMEMR2022-B02).
文摘The inside of the cell is closely filled with diverse biological macromolecules,which will affect various physi-ological activities in vivo.It is known that natural products have beneficial effects against the formation of advanced glycation end products(AGEs),but their inhibitory actions in macromolecular crowding environment are still poorly understood.Therefore,to investigate the influence of macromolecular crowding environment,the inhibitory effect of cichoric acid(CA)on the non-enzymatic glycation of bovine serum albumin(BSA)in the absence and presence of crowding reagents(PEG 2000 and PEG 4000)was studied.The results demonstrated that CA could significantly inhibit the formation of AGEs than aminoguanidine in the BSA-fructose model with or without PEG,and the inhibition effect in PBS solution was better than that in macromolecular crowding envi-ronment.Moreover,CA presented strong antioxidant capacity and trapping ability of glyoxal,and could stabilize the native structure of BSA caused by the glycation modification,which might be the primary mechanisms for its anti-glycation effect.Additionally,CA tended to bind to site I of BSA mainly through hydrogen bonds and hy-drophobic interactions.These findings indicated that CA could be regarded as a high potential anti-glycation inhibitor to prevent the development of diabetic complications.
基金financial support of the industry special project for the public welfare of the State Administration of Traditional Chinese Medicine of China(00104296)。
文摘Objective:To establish HPLC fingerprints of different parts of chicory stems,leaves,roots,flowers and seeds,and compare the similarities and differences of chemical components in different parts,so as to provide a scientific basis for the comprehensive utilization of chicory.Methods:To establish the HPLC fingerprint of chicory,the chromatographic column was chosen with Agilent ZORBAX Eclipse XDB-C_(18),the mobile phase was methanol(A)-0.2%formic acid(B),the flow rate was 1 mL/min,the column temperature was 30℃,and the detection wavelength was 254 nm.The Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine(2012 Edition)was used to evaluate the similarity of different parts of decoction pieces,and the determination method of multi-component content was established based on fingerprint identification chromatographic peaks,and the determination results were analyzed.Results:The HPLC fingerprinting method of chicory was established.Sixteen chromatographic peaks were identified and 10 of them were identified as:caftaric acid(1),esculin(2),chlorogenic acid(3),esculetin(4),caffeic acid(5),cichoric acid(8),hyperoside(11),rutin(12),isochlorogenic acid C(14)and luteolin(16).The similarity of different parts was 0.084–0.701.At the same time,the total content of detected chemical components was ranked as flower>leaf>stem>root>seed.Roots did not contain caftaric acid,rutin,and luteolin,flowers did not contain luteolin,and seeds did not contain caftaric acid,cichoric acid,and luteolin.The content of cichoric acid in leaves was the most,and esculin in flowers was the most.Conclusion:The results of HPLC fingerprint and multi-component content determination revealed the similarity and difference of different parts of chicory from chemical composition,indicating that there were certain differences in different parts of chicory.The established HPLC fingerprinting method can provide a reference for quality control and evaluation of different parts of the chicory.
