In order to look for Trichderma strains which have inhibiting effects on Cytospora chrysosperma, total 29 domestic and foreign Trichderma strains were screened by confrontation culture and growth rate method. The phys...In order to look for Trichderma strains which have inhibiting effects on Cytospora chrysosperma, total 29 domestic and foreign Trichderma strains were screened by confrontation culture and growth rate method. The physiological ecology characteristics and taxonomic statuses of the screened highly efficient antifungal strains were studied. The results showed that the n-butanol extract from T-33 fer- mentation liquid showed the highest inhibition rate （94.2%） against mycelial growth, and its inhibition rate against spore germination was up to 100%. For the growth of T-33 strain, the optimum carbon source was glucose with optimum content of 3% （g/L）; the optimum nitrogen source was soy four with optimum content of 0.02% （g/L）; the optimum initial pH of culture medium was 6; the optimum culture temper- ature was 25 ℃. The 18S rDNA sequencing results showed that the full length of ITS sequence was 583 bp, and the T-33 strain was verified as Trichoderma viride.展开更多
The pathogen Cytospora chrysosperma is the causal agent of poplar canker disease and causes considerable economic losses in China.Mitogen-activated protein kinase(MAPK)cascades play a crucial role in mediating cellula...The pathogen Cytospora chrysosperma is the causal agent of poplar canker disease and causes considerable economic losses in China.Mitogen-activated protein kinase(MAPK)cascades play a crucial role in mediating cellular responses and Pmk1-MAPKs are indispensable for pathogenic related processes in plant pathogenic fungi.In previous studies,we demonstrated that the CcPmk1 acts as a core regulator of fungal pathogenicity by modulating a small number of master downstream targets,such as CcSte12.In this study,we identified and characterized two upstream components of CcPmk1:MAPKKK CcSte11 and MAPKK CcSte7.Deletion of CcSte11 and CcSte7,resulted in slowed growth,loss of sporulation and virulence,similar to the defects observed in the CcPmk1 deletion mutant.In addition,CcSte11,CcSte7 and CcPmk1 interact with each other,and the upstream adaptor protein CcSte50 interact with CcSte11 and CcSte7.Moreover,we explored the global regulation network of CcSte12 by transcriptional analysis between CcSte12 deletion mutants and wild-type during the simulated infection process.Two hydrolase activity GO terms(GO:0004553 and GO:0016798)and starch and sucrose metabolism(mgr00500)KEGG pathway were significantly enriched in the down-regulated genes of CcSte12 deletion mutants.In addition,a subset of glycosyl hydrolase genes and putative effector genes were significantly down-regulated in the CcSte12 deletion mutant,which might be important for fungal pathogenicity.Especially,CcSte12 bound to the CcSp84 promoter region containing the TGA AAC A motif.Moreover,comparison of CcSte12-regulated genes with CcPmk1-regulated genes revealed 116 overlapping regulated genes in both CcSte12 and CcPmk1,including some virulence-associated genes.Taken together,the protein complexes CcSte11-CcSte7-CcPmk1 receive signals transmitted by upstream CcSte50 and transmit signals to downstream CcSte12,which regulates hydrolase,effectors and other genes to promote virulence.Overall,these results indicate that the CcPmk1-MAPK signaling pathway of C.chrysosperma plays a key role in the pathogenicity.展开更多
采用组织分离法对新疆南疆农田防护林主要树种腐烂病的病原菌进行分离,用形态学和分子生物学对病原菌进行鉴定。结果表明:从新疆杨Populus alba cv.pyramidalis,箭杆杨Populus nigra cv.Afghanica,胡杨Populus euphratia,柳树Salix mats...采用组织分离法对新疆南疆农田防护林主要树种腐烂病的病原菌进行分离,用形态学和分子生物学对病原菌进行鉴定。结果表明:从新疆杨Populus alba cv.pyramidalis,箭杆杨Populus nigra cv.Afghanica,胡杨Populus euphratia,柳树Salix matsudana上共分离得到52个菌株,鉴定出3种病原菌分别为金黄壳囊孢Cytospora chrysosperma(有性型Valsa sordida),C.nivea(有性型Leucostoma niveum)和Cryptosphaeria pullmanensis,其中Cytospora chrysosperma为主要种。展开更多
【目的】核桃腐烂病已成为制约新疆核桃产业健康发展的重要病害之一,筛选拮抗菌株开展该病害的生物防治,有利于新疆生态健康果园的建设。【方法】采用平板分离、对峙培养、致畸菌丝形态观察、拮抗菌形态学、生理生化特性及分子生物学的...【目的】核桃腐烂病已成为制约新疆核桃产业健康发展的重要病害之一,筛选拮抗菌株开展该病害的生物防治,有利于新疆生态健康果园的建设。【方法】采用平板分离、对峙培养、致畸菌丝形态观察、拮抗菌形态学、生理生化特性及分子生物学的鉴定方法。【结果】获得具有广谱拮抗效果的拮抗菌株XJAU-117,对核桃树腐烂病病原菌的拮抗效果达85%。通过形态学观察、生理生化特性和16s r DNA序列分析,鉴定为短小芽孢杆菌(Bacillus pumilus),拮抗菌株对核桃树腐烂病病原菌的抑制机理主要为使正常菌丝发生断裂、解体,无法正常生长。【结论】所获得的拮抗细菌对核桃树腐烂病病原菌表现出较好的拮抗作用,对生物防治核桃树腐烂病具有潜在的应用价值。展开更多
【目的】明确新疆南疆核桃腐烂病菌种类。【方法】从南疆3个地方采集核桃腐烂病样10份,采用组织分离法分离获得10个分离物,根据科赫氏法则进行致病性测定,结合形态学观察和18 S rDNA-ITS、β-tubulin两个基因序列分析。【结果】经形态...【目的】明确新疆南疆核桃腐烂病菌种类。【方法】从南疆3个地方采集核桃腐烂病样10份,采用组织分离法分离获得10个分离物,根据科赫氏法则进行致病性测定,结合形态学观察和18 S rDNA-ITS、β-tubulin两个基因序列分析。【结果】经形态学特征鉴定,菌落形态、分生孢子器、分生孢子与已报道的金黄壳囊孢Cytospora chrysosperma一致;从18 S rDNA-ITS、β-tubulin两个基因序列分析可以得出,18 S r DNA-ITS序列分析结果与C.chrysosperma(登录号:KP114125.1)相似性为99%,β-tubulin序列分析结果与C.chrysosperma(登录号:KP117038.1)相似性达到98%。【结论】新疆南疆核桃树腐烂病菌为金黄壳囊孢C.chrysosperma。展开更多
对新疆塔里木河下游胡杨树Populus euphratica腐烂病病原菌进行分离和鉴定。从新疆巴音郭楞蒙古自治州铁门关市采集胡杨树腐烂病典型病样12份,采用组织分离法分离获得12个分离物,根据科赫氏法则测定致病性,结合形态学观察和18S r DNA-I...对新疆塔里木河下游胡杨树Populus euphratica腐烂病病原菌进行分离和鉴定。从新疆巴音郭楞蒙古自治州铁门关市采集胡杨树腐烂病典型病样12份,采用组织分离法分离获得12个分离物,根据科赫氏法则测定致病性,结合形态学观察和18S r DNA-ITS,β-tubulin两个基因序列分析,将其鉴定为金黄壳囊孢Cytospora chrysosperma。展开更多
基金Supported by 948 Program of State Forestry Administration,China(2009-4-29)Postdoctoral Fund of Heilongjiang Province(LBH-Z14206)~~
文摘In order to look for Trichderma strains which have inhibiting effects on Cytospora chrysosperma, total 29 domestic and foreign Trichderma strains were screened by confrontation culture and growth rate method. The physiological ecology characteristics and taxonomic statuses of the screened highly efficient antifungal strains were studied. The results showed that the n-butanol extract from T-33 fer- mentation liquid showed the highest inhibition rate （94.2%） against mycelial growth, and its inhibition rate against spore germination was up to 100%. For the growth of T-33 strain, the optimum carbon source was glucose with optimum content of 3% （g/L）; the optimum nitrogen source was soy four with optimum content of 0.02% （g/L）; the optimum initial pH of culture medium was 6; the optimum culture temper- ature was 25 ℃. The 18S rDNA sequencing results showed that the full length of ITS sequence was 583 bp, and the T-33 strain was verified as Trichoderma viride.
基金supported by This work was supported by the National Key Research and Development Program(2022YFD1401003)the Fundamental Research Funds for the Central Universities(2021ZY15)the National Natural Science Foundation of China(31800540)。
文摘The pathogen Cytospora chrysosperma is the causal agent of poplar canker disease and causes considerable economic losses in China.Mitogen-activated protein kinase(MAPK)cascades play a crucial role in mediating cellular responses and Pmk1-MAPKs are indispensable for pathogenic related processes in plant pathogenic fungi.In previous studies,we demonstrated that the CcPmk1 acts as a core regulator of fungal pathogenicity by modulating a small number of master downstream targets,such as CcSte12.In this study,we identified and characterized two upstream components of CcPmk1:MAPKKK CcSte11 and MAPKK CcSte7.Deletion of CcSte11 and CcSte7,resulted in slowed growth,loss of sporulation and virulence,similar to the defects observed in the CcPmk1 deletion mutant.In addition,CcSte11,CcSte7 and CcPmk1 interact with each other,and the upstream adaptor protein CcSte50 interact with CcSte11 and CcSte7.Moreover,we explored the global regulation network of CcSte12 by transcriptional analysis between CcSte12 deletion mutants and wild-type during the simulated infection process.Two hydrolase activity GO terms(GO:0004553 and GO:0016798)and starch and sucrose metabolism(mgr00500)KEGG pathway were significantly enriched in the down-regulated genes of CcSte12 deletion mutants.In addition,a subset of glycosyl hydrolase genes and putative effector genes were significantly down-regulated in the CcSte12 deletion mutant,which might be important for fungal pathogenicity.Especially,CcSte12 bound to the CcSp84 promoter region containing the TGA AAC A motif.Moreover,comparison of CcSte12-regulated genes with CcPmk1-regulated genes revealed 116 overlapping regulated genes in both CcSte12 and CcPmk1,including some virulence-associated genes.Taken together,the protein complexes CcSte11-CcSte7-CcPmk1 receive signals transmitted by upstream CcSte50 and transmit signals to downstream CcSte12,which regulates hydrolase,effectors and other genes to promote virulence.Overall,these results indicate that the CcPmk1-MAPK signaling pathway of C.chrysosperma plays a key role in the pathogenicity.
文摘【目的】核桃腐烂病已成为制约新疆核桃产业健康发展的重要病害之一,筛选拮抗菌株开展该病害的生物防治,有利于新疆生态健康果园的建设。【方法】采用平板分离、对峙培养、致畸菌丝形态观察、拮抗菌形态学、生理生化特性及分子生物学的鉴定方法。【结果】获得具有广谱拮抗效果的拮抗菌株XJAU-117,对核桃树腐烂病病原菌的拮抗效果达85%。通过形态学观察、生理生化特性和16s r DNA序列分析,鉴定为短小芽孢杆菌(Bacillus pumilus),拮抗菌株对核桃树腐烂病病原菌的抑制机理主要为使正常菌丝发生断裂、解体,无法正常生长。【结论】所获得的拮抗细菌对核桃树腐烂病病原菌表现出较好的拮抗作用,对生物防治核桃树腐烂病具有潜在的应用价值。
文摘【目的】明确新疆南疆核桃腐烂病菌种类。【方法】从南疆3个地方采集核桃腐烂病样10份,采用组织分离法分离获得10个分离物,根据科赫氏法则进行致病性测定,结合形态学观察和18 S rDNA-ITS、β-tubulin两个基因序列分析。【结果】经形态学特征鉴定,菌落形态、分生孢子器、分生孢子与已报道的金黄壳囊孢Cytospora chrysosperma一致;从18 S rDNA-ITS、β-tubulin两个基因序列分析可以得出,18 S r DNA-ITS序列分析结果与C.chrysosperma(登录号:KP114125.1)相似性为99%,β-tubulin序列分析结果与C.chrysosperma(登录号:KP117038.1)相似性达到98%。【结论】新疆南疆核桃树腐烂病菌为金黄壳囊孢C.chrysosperma。
文摘对新疆塔里木河下游胡杨树Populus euphratica腐烂病病原菌进行分离和鉴定。从新疆巴音郭楞蒙古自治州铁门关市采集胡杨树腐烂病典型病样12份,采用组织分离法分离获得12个分离物,根据科赫氏法则测定致病性,结合形态学观察和18S r DNA-ITS,β-tubulin两个基因序列分析,将其鉴定为金黄壳囊孢Cytospora chrysosperma。
