Globally,approximately 10 million new tuberculosis(TB)cases are reported annually.Delayed diagnosis due to low detection rates is the primary cause of mortality.Although pathological examination is commonly used for d...Globally,approximately 10 million new tuberculosis(TB)cases are reported annually.Delayed diagnosis due to low detection rates is the primary cause of mortality.Although pathological examination is commonly used for diagnosing TB,5%-30%of cases remain undiagnosed,emphasizing the urgent need to establish quality control(QC)standards to reduce rates of misdiagnosis and missed diagnoses.To address this,we introduced a novel QC chip for detecting Mycobacterium tuberculosis(MTB).A quantitative pathological QC model was constructed by precisely and uniformly integrating MTB and HeLa cells into a photocurable hydrogel.This model was then sliced into uniform sections to create QC chips.It demonstrated that the QC chips exhibited no significant differences in intra-batch or inter-batch variation(coefficient of variation<5%),and remained stable at−80°C for one year.Furthermore,these chips were found to be 100%effective when tested with 240 clinical samples(200 with special staining and 40 with polymerase chain reaction).In addition to enhancing TB detection rates,this approach offers visualization,quantification,and sustainable production.Overall,this work provides a novel framework for developing QC chips for pathological testing,offering a reliable solution to enhance clinical diagnostic workflows.展开更多
A pressure force control system for hot embossing of microfluidic chips is designed with a moment motor and a ball bearing lead screw. Based on the numeric PID technique, the algorithm of pulsant integral accelerated ...A pressure force control system for hot embossing of microfluidic chips is designed with a moment motor and a ball bearing lead screw. Based on the numeric PID technique, the algorithm of pulsant integral accelerated PID control is presented and the negative effects of nonlinearity from friction, clearance and saturation are eliminated. In order to improve the quick-resixmse characteristic, independent thread technique is adopted. The method of pressure force control based on pulsant integral accelerated PID control and independent thread technique is applied with satisfactory control performance.展开更多
Fibres are being rapidly developed into intelligent devices and systems.Through the integration of microelectronic chips and controllers within individual fibres,these systems can now perform advanced functionalities ...Fibres are being rapidly developed into intelligent devices and systems.Through the integration of microelectronic chips and controllers within individual fibres,these systems can now perform advanced functionalities including sensing,data storage,computational processing,and wireless communication-all integrated into a single fibre.Recently,Fink et al.demonstrated a textile-integrated fibre computer that achieves these multifunctional capabilities while weighing less than 5 g.This breakthrough work provides novel design paradigms for the integration of fibres and electronics,transcending the conventional functional limitations of individual fibres and establishing new research directions in computational textiles.展开更多
基金supported by the National Natural Science Foundation of China(Nos.52325504 and 52235007)the Basic Public Welfare Research Project of Zhejiang Province(No.LGY23H160089)the Science and Technology Plan Project of Taizhou City(No.24ywa08).
文摘Globally,approximately 10 million new tuberculosis(TB)cases are reported annually.Delayed diagnosis due to low detection rates is the primary cause of mortality.Although pathological examination is commonly used for diagnosing TB,5%-30%of cases remain undiagnosed,emphasizing the urgent need to establish quality control(QC)standards to reduce rates of misdiagnosis and missed diagnoses.To address this,we introduced a novel QC chip for detecting Mycobacterium tuberculosis(MTB).A quantitative pathological QC model was constructed by precisely and uniformly integrating MTB and HeLa cells into a photocurable hydrogel.This model was then sliced into uniform sections to create QC chips.It demonstrated that the QC chips exhibited no significant differences in intra-batch or inter-batch variation(coefficient of variation<5%),and remained stable at−80°C for one year.Furthermore,these chips were found to be 100%effective when tested with 240 clinical samples(200 with special staining and 40 with polymerase chain reaction).In addition to enhancing TB detection rates,this approach offers visualization,quantification,and sustainable production.Overall,this work provides a novel framework for developing QC chips for pathological testing,offering a reliable solution to enhance clinical diagnostic workflows.
基金This project is supported by National Hi-tech Research and Development Program of China (863 Program, No. 2004AA404260).
文摘A pressure force control system for hot embossing of microfluidic chips is designed with a moment motor and a ball bearing lead screw. Based on the numeric PID technique, the algorithm of pulsant integral accelerated PID control is presented and the negative effects of nonlinearity from friction, clearance and saturation are eliminated. In order to improve the quick-resixmse characteristic, independent thread technique is adopted. The method of pressure force control based on pulsant integral accelerated PID control and independent thread technique is applied with satisfactory control performance.
基金the National Natural Science Foundation of China(Grant No.52402230,52127805 52202167)Chenguang Program of Shanghai Education Development Foundation and Shanghai Municipal Education Commission(23CGA41)+1 种基金Fundamental Research Funds for the Central Universities(2232024D-02)Natural Science Foundation of Shanghai(24ZR1402300).
文摘Fibres are being rapidly developed into intelligent devices and systems.Through the integration of microelectronic chips and controllers within individual fibres,these systems can now perform advanced functionalities including sensing,data storage,computational processing,and wireless communication-all integrated into a single fibre.Recently,Fink et al.demonstrated a textile-integrated fibre computer that achieves these multifunctional capabilities while weighing less than 5 g.This breakthrough work provides novel design paradigms for the integration of fibres and electronics,transcending the conventional functional limitations of individual fibres and establishing new research directions in computational textiles.
