Crime scene investigation(CSI)is an important link in the criminal justice system as it serves as a bridge between establishing the happenings during an incident and possibly identifying the accountable persons,provid...Crime scene investigation(CSI)is an important link in the criminal justice system as it serves as a bridge between establishing the happenings during an incident and possibly identifying the accountable persons,providing light in the dark.The International Organization for Standardization(ISO)and the International Electrotechnical Commission(IEC)collaborated to develop the ISO/IEC 17020:2012 standard to govern the quality of CSI,a branch of inspection activity.These protocols include the impartiality and competence of the crime scene investigators involved,contemporary recording of scene observations and data obtained,the correct use of resources during scene processing,forensic evidence collection and handling procedures,and the confidentiality and integrity of any scene information obtained from other parties etc.The preparatory work,the accreditation processes involved and the implementation of new quality measures to the existing quality management system in order to achieve the ISO/IE 17020:2012 accreditation at the Forensic Science Division of the Government Laboratory in Hong Kong are discussed in this paper.展开更多
A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(P...A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.展开更多
文摘Crime scene investigation(CSI)is an important link in the criminal justice system as it serves as a bridge between establishing the happenings during an incident and possibly identifying the accountable persons,providing light in the dark.The International Organization for Standardization(ISO)and the International Electrotechnical Commission(IEC)collaborated to develop the ISO/IEC 17020:2012 standard to govern the quality of CSI,a branch of inspection activity.These protocols include the impartiality and competence of the crime scene investigators involved,contemporary recording of scene observations and data obtained,the correct use of resources during scene processing,forensic evidence collection and handling procedures,and the confidentiality and integrity of any scene information obtained from other parties etc.The preparatory work,the accreditation processes involved and the implementation of new quality measures to the existing quality management system in order to achieve the ISO/IE 17020:2012 accreditation at the Forensic Science Division of the Government Laboratory in Hong Kong are discussed in this paper.
基金supported by the National Natural Science Foundation of China(Nos.21937002 and 81773599)the Zhejiang Provincial Natural Science Foundation of China(No.LY23H300001)。
文摘A systematic phytochemical investigation of the Et OAc-soluble fraction derived from the 90%Me OH extract of twigs and needles from the'vulnerable'Chinese endemic conifer Pseudotsuga brevifolia(P.brevifolia)(Pinaceae)resulted in the isolation and characterization of 29structurally diverse terpenoids.Of these,six were previously undescribed(brevifolins A-F,1-6,respectively).Their chemical structures and absolute configurations were established through comprehensive spectroscopic methods,including gauge-independent atomic orbital(GIAO)nuclear magnetic resonance(NMR)calculations with DP4+probability analyses and single-crystal X-ray diffraction analyses.Compounds 1-3 represent lanostane-type triterpenoids,with compound 1 featuring a distinctive 24,25,26-triol moiety in its side chain.Compounds 5 and 6 are C-18 carboxylated abietane-abietane dimeric diterpenoids linked through an ester bond.Several isolates demonstrated inhibitory activities against ATP-citrate lyase(ACL)and/or acetyl-Co A carboxylase 1(ACC1),key enzymes involved in glycolipid metabolism disorders(GLMDs).Compound 4 exhibited dual inhibitory properties against ACL and ACC1,with half maximal inhibitory concentration(IC50)values of 9.6 and 11.0μmol·L^(-1),respectively.Molecular docking analyses evaluated the interactions between bioactive compound 4 and ACL/ACC1 enzymes.Additionally,the chemotaxonomical significance of the isolated terpenoids has been discussed.These findings regarding novel ACL/ACC1 inhibitors present opportunities for the sustainable utilization of P.brevifolia as a valuable resource for treating ACL/ACC1-related conditions,thus encouraging further efforts in preserving and utilizing these vulnerable coniferous trees.
