Previous study indicated that the thermo-sensitive genic malesterile(TGMS)gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS syste...Previous study indicated that the thermo-sensitive genic malesterile(TGMS)gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS)system.'AnnongS'is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1'and'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1'×'Nanjing11','Y58S'×'Q611',and'Y58S'×'Guanghui122')were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023)of the NAC(NAM-ATAF-CUC-related)gene family was identified as the candidate gene of the tms5 gene.展开更多
文摘Previous study indicated that the thermo-sensitive genic malesterile(TGMS)gene in rice was regulated by temperature.TGMS rice plays an important role in hybrid rice production,because the application of the TGMS system in two-line breeding is laborsaving,timesaving,simple,inexpensive,efficient,and eliminating the limitations of the cytoplasmic male sterility(CMS)system.'AnnongS'is the first discovered and deeply studied TGMS rice lines in China.'AnnongS-1'and'Y58S',two derivatives of TGMS line AnnongS,were both controlled by a single recessive gene named tms5,which was genetically mapped on chromosome 2.In this study,three populations('AnnongS-1'×'Nanjing11','Y58S'×'Q611',and'Y58S'×'Guanghui122')were developed and used for the molecular fine mapping of the tms5 gene.By analyzing recombination events in the sterile individuals using a total of 125 probes covering the tms5 region,the tms5 gene was physically mapped to a 19-kb DNA fragment between two markers 4039-1 and 4039-2,which were located on the BAC clone AP004039.After the construction of the physical map between two markers 4039-1 and 4039-2,a member(ONAC023)of the NAC(NAM-ATAF-CUC-related)gene family was identified as the candidate gene of the tms5 gene.
