The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants w...The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.展开更多
The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnorm...The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.展开更多
Since the establishment of the Hollywood film industry system,character portrayal has always been a core issue in narrative creation.From the rebellious Scarlett in Gone with the Wind,to Andy who adheres to hope in Th...Since the establishment of the Hollywood film industry system,character portrayal has always been a core issue in narrative creation.From the rebellious Scarlett in Gone with the Wind,to Andy who adheres to hope in The Shawshank Redemption,and then to Arthur who succumbs to madness and despair in Joker,American films have constructed emotional connections across cultural barriers through a series of vivid character images.Traditional character analysis mostly focuses on characters'behavioral performances and personality labels,but ignores the logic of character formation constructed by screenwriters behind the scenes.This paper proposes a three-dimensional analytical framework of"Concealment-Conflict-Value",aiming to uncover the methodological black box of character portrayal in American films:Concealment represents the dynamic growth trajectory of characters'personalities,highlighting the iterative realization of roles;Conflict,as a touchstone for personality traits,reveals the essence of characters through the analysis of three dimensions:self,others,and environment;Value is the soul of characters'personalities,determining the direction and realm of roles.The results indicate that applying this framework to analyze classic characters in American films can not only extract replicable creative experiences,but also offer a glimpse into the understanding of human nature and spiritual demands within the context of American culture.展开更多
Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitorin...Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitoring.Impact Statement:The integration of Ti_(3)C_(2)T_(x)-MXene into BC matrices represents a novel approach to creating multifunctional hydrogels that combine biocompatibility,electrical conductivity,and mechanical durability.These properties make the hydrogels promising candidates for advanced wound care and real-time monitoring applications.Introduction:Wound healing requires materials that support cell growth,promote tissue regeneration,and enable real-time monitoring.MXenes,a class of 2-dimensional materials,offer unique electrical and mechanical properties,making them suitable for biomedical applications.This study explores the integration of Ti_(3)C_(2)T_(x)-MXene with BC,a biopolymer known for its excellent biocompatibility and mechanical strength,to create electroactive composite hydrogel films for advanced wound care.Methods:Ti_(3)C_(2)T_(x)-MXene was synthesized by etching Ti3AlC2 with hydrofluoric acid and integrated into BC pellicles produced by Gluconacetobacter xylinum.The composite hydrogel films underwent characterization through x-ray diffraction(XRD),x-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and thermogravimetric analysis(TGA)to determine structural,chemical,and thermal properties.Mechanical testing assessed tensile and compressive strengths.Biological assessments,including cell viability,hemolysis rate,and protein expression,evaluated biocompatibility and regenerative potential.Results:XRD confirmed the crystallographic structure of MXene and BC composite film.XPS and FTIR validated the successful incorporation of MXene into the film matrix.Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive strength of 4.2 MPa.TGA showed stability up to 350℃,and the electrical conductivity reached 9.14×10^(−4)S/m,enabling real-time monitoring capabilities.Cell viability exceeded 95%,with a hemolysis rate below 2%.Protein expression studies revealed the ability to promote skin regeneration through collagen I,K10,K5,and filaggrin expression.Conclusion:The BC/MXene composite hydrogel films exhibit important potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring.Their unique combination of mechanical durability,electrical conductivity,and biocompatibility highlights their promise for advanced wound care applications.展开更多
In a study published recently in Nature,Sass,Ma,and colleagues describe the neurokinin 2 receptor(NK2R),a G protein-coupled receptor(GPCR),as a novel regulator of food intake as well as energy expenditure,and develop ...In a study published recently in Nature,Sass,Ma,and colleagues describe the neurokinin 2 receptor(NK2R),a G protein-coupled receptor(GPCR),as a novel regulator of food intake as well as energy expenditure,and develop and characterize selective agonists that effectively activate NK2R to promote weight loss.Most interestingly,the authors bridge the gap between rodents and primates,raising hopes for novel treatment options.1 Although obesity cases are continuously rising,thereby contributing to major health problems,long-term effective treatment options are still limited.However,it is well established that GPCRs and their pathways are involved in the regulation of different processes controlling appetite,food intake,or energy homeostasis.Thus,several receptors expressed in neurons governing food intake have been proposed to be promising targets for body weight reduction.However,currently the only GPCR agonists approved for obesity treatment are targeting incretin receptors(receptors for glucagon-like peptide-1(GLP1R),glucose-dependent insulinotropic peptide(GIP),glucagon(GCGR))(Fig.1).展开更多
1.INTRODUCTION First described in 1964,46,XX male syndrome is a rare disorder of sex development(DSD)characterized by a discordance between a female karyotype and male phenotype.1,2 Its prevalence is estimated to be 3...1.INTRODUCTION First described in 1964,46,XX male syndrome is a rare disorder of sex development(DSD)characterized by a discordance between a female karyotype and male phenotype.1,2 Its prevalence is estimated to be 3 to 4 cases per 100,000 live male births.3,4 Clinically,the affected individuals can be grouped into three categories:phenotypically normal males with infertility,males with genital ambiguity,and true hermaphrodites.Previous reports have linked this condition to autoimmune thyroiditis and myelodysplastic syndromes.展开更多
基金the National Natural science Fundationof china(39970386)
文摘The rol genes cloned from Agrobacterium rhizogenes were transferred to the cotton genomevia Agrobacterium-mediated transformation. Molecular analyses and developmentalidentification of the putative transgenic plants were carried out by means of PCR,Southern blotting and field characterization. The results showed that the expression ofrol genes greatly increased the rooting ability of the transgenic plants, and changed theplant development. Highly male-sterile plants with strong apical dominance and fertileplants with short internodes, stunted growth and improved economic characteristics weresegregated from the T1 transgenic lines of wild rol B gene and the rol B gene driven by35S promoter. The transgenic lines of rol ABC construct usually had normal boll settingand slow growth. Therefore we concluded that the rol genes, modified in suitable ways,could be used to create new cotton varieties with some highly valuable characteristics.
基金supported by the National Science & Technology Pillar Program of China in the Tenth Five-Year Plan Period(Grant No.2001BA302B)the Education Department of Henan Province, China(Grant No.2008A208019)
文摘The process of double fertilization and the characters of embryo and endosperm development in an autotetraploid polyembryonic mutant rice IR36-Shuang were studied with a laser scanning confocal microscopy. Some abnormalities including degenerated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo and so on. were found dudng double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67% in IR36-Shuang, significantly higher than that in the control, an autotetraploid rice line IR36-4X （33.00%）. Cytological and embryonic evidences were provided for seed setting decline and the initiation of additional embryo in IR36-Shuang.
文摘Since the establishment of the Hollywood film industry system,character portrayal has always been a core issue in narrative creation.From the rebellious Scarlett in Gone with the Wind,to Andy who adheres to hope in The Shawshank Redemption,and then to Arthur who succumbs to madness and despair in Joker,American films have constructed emotional connections across cultural barriers through a series of vivid character images.Traditional character analysis mostly focuses on characters'behavioral performances and personality labels,but ignores the logic of character formation constructed by screenwriters behind the scenes.This paper proposes a three-dimensional analytical framework of"Concealment-Conflict-Value",aiming to uncover the methodological black box of character portrayal in American films:Concealment represents the dynamic growth trajectory of characters'personalities,highlighting the iterative realization of roles;Conflict,as a touchstone for personality traits,reveals the essence of characters through the analysis of three dimensions:self,others,and environment;Value is the soul of characters'personalities,determining the direction and realm of roles.The results indicate that applying this framework to analyze classic characters in American films can not only extract replicable creative experiences,but also offer a glimpse into the understanding of human nature and spiritual demands within the context of American culture.
文摘Objective:This study aims to develop and characterize electroactive hydrogels based on reduced bacterial cellulose(BC)and Ti_(3)C_(2)T_(x)-MXene for their potential application in wound healing and real-time monitoring.Impact Statement:The integration of Ti_(3)C_(2)T_(x)-MXene into BC matrices represents a novel approach to creating multifunctional hydrogels that combine biocompatibility,electrical conductivity,and mechanical durability.These properties make the hydrogels promising candidates for advanced wound care and real-time monitoring applications.Introduction:Wound healing requires materials that support cell growth,promote tissue regeneration,and enable real-time monitoring.MXenes,a class of 2-dimensional materials,offer unique electrical and mechanical properties,making them suitable for biomedical applications.This study explores the integration of Ti_(3)C_(2)T_(x)-MXene with BC,a biopolymer known for its excellent biocompatibility and mechanical strength,to create electroactive composite hydrogel films for advanced wound care.Methods:Ti_(3)C_(2)T_(x)-MXene was synthesized by etching Ti3AlC2 with hydrofluoric acid and integrated into BC pellicles produced by Gluconacetobacter xylinum.The composite hydrogel films underwent characterization through x-ray diffraction(XRD),x-ray photoelectron spectroscopy(XPS),Fourier transform infrared spectroscopy(FTIR),and thermogravimetric analysis(TGA)to determine structural,chemical,and thermal properties.Mechanical testing assessed tensile and compressive strengths.Biological assessments,including cell viability,hemolysis rate,and protein expression,evaluated biocompatibility and regenerative potential.Results:XRD confirmed the crystallographic structure of MXene and BC composite film.XPS and FTIR validated the successful incorporation of MXene into the film matrix.Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive strength of 4.2 MPa.TGA showed stability up to 350℃,and the electrical conductivity reached 9.14×10^(−4)S/m,enabling real-time monitoring capabilities.Cell viability exceeded 95%,with a hemolysis rate below 2%.Protein expression studies revealed the ability to promote skin regeneration through collagen I,K10,K5,and filaggrin expression.Conclusion:The BC/MXene composite hydrogel films exhibit important potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring.Their unique combination of mechanical durability,electrical conductivity,and biocompatibility highlights their promise for advanced wound care applications.
基金supported by the German Diabetes Association(DDG)(D.T.)the German Research Foundation(DFG)in CRC1423/2(project number 421152132,C04(S.P.)).
文摘In a study published recently in Nature,Sass,Ma,and colleagues describe the neurokinin 2 receptor(NK2R),a G protein-coupled receptor(GPCR),as a novel regulator of food intake as well as energy expenditure,and develop and characterize selective agonists that effectively activate NK2R to promote weight loss.Most interestingly,the authors bridge the gap between rodents and primates,raising hopes for novel treatment options.1 Although obesity cases are continuously rising,thereby contributing to major health problems,long-term effective treatment options are still limited.However,it is well established that GPCRs and their pathways are involved in the regulation of different processes controlling appetite,food intake,or energy homeostasis.Thus,several receptors expressed in neurons governing food intake have been proposed to be promising targets for body weight reduction.However,currently the only GPCR agonists approved for obesity treatment are targeting incretin receptors(receptors for glucagon-like peptide-1(GLP1R),glucose-dependent insulinotropic peptide(GIP),glucagon(GCGR))(Fig.1).
基金supported by the National Natural Science Foundation of China(82200193,82572140).
文摘1.INTRODUCTION First described in 1964,46,XX male syndrome is a rare disorder of sex development(DSD)characterized by a discordance between a female karyotype and male phenotype.1,2 Its prevalence is estimated to be 3 to 4 cases per 100,000 live male births.3,4 Clinically,the affected individuals can be grouped into three categories:phenotypically normal males with infertility,males with genital ambiguity,and true hermaphrodites.Previous reports have linked this condition to autoimmune thyroiditis and myelodysplastic syndromes.
