Sorting of desired single cells from a cell population is crucial for many applications in biology and biomedicine that require analysis at the cellular level.Microfluidic dielectrophoresis(DEP)-based single-cell sort...Sorting of desired single cells from a cell population is crucial for many applications in biology and biomedicine that require analysis at the cellular level.Microfluidic dielectrophoresis(DEP)-based single-cell sorting method has been demonstrated as a powerful technology to enable high-throughput and accurate sorting of single cells.However,conventional DEP sorting is mainly performed in the oil phase constrained by solid channels,which restricts the capacity and tunability of the sorting path.Here,we describe an approach to sort single cells on multiple paths in air.Our device allows for tunable ejection of droplets containing single cells in air,which are interrogated and sorted by a microfluidic DEP sorter with a cylindrical electrode,showing a sorting accuracy of>99%for all paths with high survival rates.We demonstrate the utility of our device by isolating multiple subpopulations from a cell sample with three types of cells.Our technology holds the potential to perform sorting on numerous paths,making it applicable for multipurpose sorting from complex heterogeneous cell populations.展开更多
To the Editor,We read with interest the article by Lehrer et al.on an in silico molecular docking and molecular dynamics simulation study with Cx43 channels,which investigated whether insulin or metformin can dock wit...To the Editor,We read with interest the article by Lehrer et al.on an in silico molecular docking and molecular dynamics simulation study with Cx43 channels,which investigated whether insulin or metformin can dock within the Cx43 channel and effectively block it,thereby reducing the risk of amyotrophic lateral sclerosis(ALS)[1].Based on data from the FDA Med Watch,it was found that the insulin heterodimer docks in the center of the Cx43 channel and effectively blocks it,and that this blockade is very stable,thereby achieving a protective effect on ALS[1].It has been found that metformin also docks in the Cx43 channel,but due to the small size of the molecule,it does not impede the passage of toxic waste products from astrocytes[1].The study is impressive,but some points should be discussed.展开更多
基金supported by the National Natural Science Foundation of China(Grant no.52475584,52205296)National Key R&D Program of China(Grant no.2025YFC3408703)Aeronautical Science Foundation of China(Grant Number 2023Z056051001).
文摘Sorting of desired single cells from a cell population is crucial for many applications in biology and biomedicine that require analysis at the cellular level.Microfluidic dielectrophoresis(DEP)-based single-cell sorting method has been demonstrated as a powerful technology to enable high-throughput and accurate sorting of single cells.However,conventional DEP sorting is mainly performed in the oil phase constrained by solid channels,which restricts the capacity and tunability of the sorting path.Here,we describe an approach to sort single cells on multiple paths in air.Our device allows for tunable ejection of droplets containing single cells in air,which are interrogated and sorted by a microfluidic DEP sorter with a cylindrical electrode,showing a sorting accuracy of>99%for all paths with high survival rates.We demonstrate the utility of our device by isolating multiple subpopulations from a cell sample with three types of cells.Our technology holds the potential to perform sorting on numerous paths,making it applicable for multipurpose sorting from complex heterogeneous cell populations.
文摘To the Editor,We read with interest the article by Lehrer et al.on an in silico molecular docking and molecular dynamics simulation study with Cx43 channels,which investigated whether insulin or metformin can dock within the Cx43 channel and effectively block it,thereby reducing the risk of amyotrophic lateral sclerosis(ALS)[1].Based on data from the FDA Med Watch,it was found that the insulin heterodimer docks in the center of the Cx43 channel and effectively blocks it,and that this blockade is very stable,thereby achieving a protective effect on ALS[1].It has been found that metformin also docks in the Cx43 channel,but due to the small size of the molecule,it does not impede the passage of toxic waste products from astrocytes[1].The study is impressive,but some points should be discussed.
