Type I interferon(IFN-I)provides an important first line to protect avian species against pathogens invasion.IFN regulatory factor 7(IRF7)has been identified as the most important regulator for both DNA and RNA virus-...Type I interferon(IFN-I)provides an important first line to protect avian species against pathogens invasion.IFN regulatory factor 7(IRF7)has been identified as the most important regulator for both DNA and RNA virus-induced IFN-I production in chickens.Although four splicing variants of IRF7 have been identified in mammals,it is still unclear whether alternative splicing patterns and the function of IRF7 isoform(s)exist in chickens.In this study,we reported a novel short transcript isoform of chicken IRF7(chIRF7),termed chIRF7-iso,which contained an intact N-terminal DNA-binding domain(DBD)and 14 amino acids different from chIRF7 in the C-terminal.Overexpression of chIRF7 in chicken leghorn male hepatocellular(LMH)cells activated the IFN-βpromoter and significantly inhibited Newcastle disease virus(NDV)and fowl adenovirus serotype 4(FAdV-4)replication.Conversely,overexpression of chIRF7-iso blocked the IFN-βpromoter activity and was favorable for NDV and FAdV-4 replication in vitro.Collectively,our results confirm that a novel chIRF7 isoform-mediated negative regulates IFN-βproduction,which will contribute to understanding the role of chIRF7 in innate antiviral response in chicken.展开更多
基金supported by the grants from the National Natural Science Foundation of China(32002259)the Natural Science Foundation of Henan Province,China(202300410198).
文摘Type I interferon(IFN-I)provides an important first line to protect avian species against pathogens invasion.IFN regulatory factor 7(IRF7)has been identified as the most important regulator for both DNA and RNA virus-induced IFN-I production in chickens.Although four splicing variants of IRF7 have been identified in mammals,it is still unclear whether alternative splicing patterns and the function of IRF7 isoform(s)exist in chickens.In this study,we reported a novel short transcript isoform of chicken IRF7(chIRF7),termed chIRF7-iso,which contained an intact N-terminal DNA-binding domain(DBD)and 14 amino acids different from chIRF7 in the C-terminal.Overexpression of chIRF7 in chicken leghorn male hepatocellular(LMH)cells activated the IFN-βpromoter and significantly inhibited Newcastle disease virus(NDV)and fowl adenovirus serotype 4(FAdV-4)replication.Conversely,overexpression of chIRF7-iso blocked the IFN-βpromoter activity and was favorable for NDV and FAdV-4 replication in vitro.Collectively,our results confirm that a novel chIRF7 isoform-mediated negative regulates IFN-βproduction,which will contribute to understanding the role of chIRF7 in innate antiviral response in chicken.
