Herpes simplex virus type 1（HSV-1） enters productive infection after infecting epithelial cells, where it controls the host nucleus to make viral proteins, starts viral DNA synthesis and assembles infectious virions...Herpes simplex virus type 1（HSV-1） enters productive infection after infecting epithelial cells, where it controls the host nucleus to make viral proteins, starts viral DNA synthesis and assembles infectious virions. In this process, replicating viral genomes are organized into replication centers to facilitate viral growth. HSV-1 is known to use host factors, including host chromatin and host transcription regulators, to transcribe its genes; however, the invading virus also encounters host defense and stress responses to inhibit viral growth. Recently, we found that HSV-1 replication centers recruit host factor CTCF but exclude γH2A.X. Thus, HSV-1 replication centers may selectively recruit cellular factors needed for viral growth, while excluding host factors that are deleterious for viral transcription or replication. Here we report that the viral replication centers selectively excluded modified histone H3, including heterochromatin mark H3K9me3, H3S10 P and active chromatin mark H3K4me3, but not unmodified H3. We found a dynamic association between the viral replication centers and host RNA polymerase II. The centers also recruited components of the DNA damage response pathway, including 53BP1, BRCA1 and host antiviral protein SP100. Importantly, we found that ATM kinase was needed for the recruitment of CTCF to the viral centers. These results suggest that the HSV-1 replication centers took advantage of host signaling pathways to actively recruit or exclude host factors to benefit viral growth.展开更多
This thesis gives the overview of concept and constitution of the closed supply chain of green agricultural products based on logistics center,and the necessity of regarding logistics center as core enterprise.Meanwhi...This thesis gives the overview of concept and constitution of the closed supply chain of green agricultural products based on logistics center,and the necessity of regarding logistics center as core enterprise.Meanwhile,it analyzes the function of logistics center of agricultural products,namely functions of exchange,collection,distribution,storage and transportation.It also poses the prerequisites of logistics center.The logistics center of agricultural products must have stable profit expectancy,prominent scale,strong appealing power,capacity of bearing market risk and basic thoughts of supply chain management.The functional design of the closed supply chain of green agricultural products has been discussed in 5 aspects,namely logistics center,production base,providers of means of production,retailer and consumer.The operating thoughts of the closed supply chain of green agricultural products based on logistics center are put forward on the basis of the research of operating objective and service target of supply chain as follows:first,based on local principal agricultural products,the logistics center mainly distributes the massed agricultural products;second,it is necessary to choose appropriate strategic cooperative partners to sign contract;third,the operating procedure of supply chain entails bringing in professional managerial talents of supply chain;finally,the relationships of supply chain should be maintained.展开更多
一般来讲,if 是最普通的连接词,表示单纯的条件或假设。但是在某些特殊情况下,if 却有其特殊用法,请看下面的例句如何理解。The necklace costs ＄250 if it costs a cent.将 if 从句看作是单纯的条件句,显然是不合逻辑的,这种情况下,if...一般来讲,if 是最普通的连接词,表示单纯的条件或假设。但是在某些特殊情况下,if 却有其特殊用法,请看下面的例句如何理解。The necklace costs ＄250 if it costs a cent.将 if 从句看作是单纯的条件句,显然是不合逻辑的,这种情况下,if 从句失去了其字面意义,有“敢肯定(敢断言)……至少……”之意,那么应翻译为“(我敢说)这条项链至少值250美元”。由此可见,if 从句并不单纯表示条件,而是在条件中寓有肯定主句主论的含义,即 if 从句的作用是:用能够类比的最小单位(如 a cent,a day,a pen-ny,an inch,an ounce,a man,once 等)来对比,强调主句中所要肯定的数字,展开更多
随着计算机信息技术的发展,云计算与大数据技术的出现成为信息产业的重大变革。云计算、物联网、移动互联网的迅速发展催生大数据时代的到来,Hadoop分布式集群的成功搭建为大数据工程研究提供了基础环境。结合虚拟机,通过基于Cent OS 7...随着计算机信息技术的发展,云计算与大数据技术的出现成为信息产业的重大变革。云计算、物联网、移动互联网的迅速发展催生大数据时代的到来,Hadoop分布式集群的成功搭建为大数据工程研究提供了基础环境。结合虚拟机,通过基于Cent OS 7的系统安装、网络配置、远程链接、集群搭建、免密登录、时间同步等过程的研究和实现,让Hadoop集群的配置更加方便、快捷。展开更多
With the popularity of Computer Assisted Language Learning(CALL),autonomous language learning centers have been established in the universities throughout the country.However,there are many problems exist in the pract...With the popularity of Computer Assisted Language Learning(CALL),autonomous language learning centers have been established in the universities throughout the country.However,there are many problems exist in the practice of the autonomous language learning center.This essay tries to discuss the problems and its countermeasures with an aim of improving College English learning and teaching by the example of autonomous language learning center in Nanyang Institute of Technology.展开更多
首都国际会展中心项目作为超大型公共建筑工程,其特点表现于钢结构体量大,结构体系和节点构造复杂多变,构件数量多且单件重量大,合理选择施工方法和施工机械是重点。以首都国际会展中心为研究对象,以保证中心项目钢结构工程进度、质量...首都国际会展中心项目作为超大型公共建筑工程,其特点表现于钢结构体量大,结构体系和节点构造复杂多变,构件数量多且单件重量大,合理选择施工方法和施工机械是重点。以首都国际会展中心为研究对象,以保证中心项目钢结构工程进度、质量为目的,通过BIM(Building Information Modeling)结合智能建造技术在钢结构生产安装全过程中的综合应用,有效降低了项目因质量问题拆改所带来的经济损失,确保了现场钢结构安装进度,节约钢结构生产过程中所带来的损耗,为类似工程提供了宝贵经验。展开更多
The rapid growth of foreign investment in China will continue and may even accelerate after the country enters the World Trade Organization (WTO), analysts said. "More fields are opening to foreign investors and ...The rapid growth of foreign investment in China will continue and may even accelerate after the country enters the World Trade Organization (WTO), analysts said. "More fields are opening to foreign investors and market regulations are becoming more transparent, which encourages foreign companies to increase their presence in China," said Sun Xiaohua, a senior researcher at the Chinese Academy of International Trade and展开更多
面向园区综合能源系统中供能方与用能方的角色互换,以及园区低碳经济运行的强约束,提出了一种考虑动态参数的阶梯型碳交易机制和需求响应的园区级综合能源系统主从博弈优化调度方法。首先,将园区级综合能源系统中能源运营商(energy syst...面向园区综合能源系统中供能方与用能方的角色互换,以及园区低碳经济运行的强约束,提出了一种考虑动态参数的阶梯型碳交易机制和需求响应的园区级综合能源系统主从博弈优化调度方法。首先,将园区级综合能源系统中能源运营商(energy system operator,ESO)设定为上层领导者、综合能源系统园区设定为下层跟随者,并且能源运营商以最大化自身效益为目标,通过制定与园区间的购售电价格、碳交易基价、价格增长幅度,引导下层园区优化;下层园区以最小化其运行成本为目标,对上层发布的价格信息做出反应,从而构建主从博弈模型。其次,充分考虑园区级综合能源系统的低碳经济运行约束,在博弈模型中引入考虑动态参数的阶梯型碳交易机制以限制二氧化碳排放量,并在园区侧引入需求响应。最后,利用水母搜索算法对上层发布的购售电价、碳交易基价、价格增长幅度进行优化,利用CPLEX优化下层园区设备出力、需求响应以及购售电计划。仿真结果证明了所提模型和方法的有效性。展开更多
目的研究腺病毒携带目的基因经小鼠耳后入路圆窗膜显微注射途径耳蜗转导的可行性,为以小鼠作为动物模型的内耳基因治疗提供实验基础和解剖学依据。方法12只C57BL/6J小鼠分为2组,实验组(8只)以重组腺病毒携带的增强型绿色荧光蛋白基因(en...目的研究腺病毒携带目的基因经小鼠耳后入路圆窗膜显微注射途径耳蜗转导的可行性,为以小鼠作为动物模型的内耳基因治疗提供实验基础和解剖学依据。方法12只C57BL/6J小鼠分为2组,实验组(8只)以重组腺病毒携带的增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP)、对照组(4只)以人工外淋巴液经耳后入路圆窗膜显微注射注入耳蜗内。分别于术后5、14天取双侧耳蜗标本做基底膜铺片,在激光共聚焦显微镜下观察GFP表达。结果术后动物存活10只(每组死亡1只)。实验组转染后耳蜗底回基底膜及螺旋神经节上目的基因有表达,14天组强于5天组。对照组耳蜗未见荧光表达。结论耳后入路操作简单、损伤小、易于暴露圆窗龛。耳后入路圆窗膜显微注射腺病毒携带目的基因转导的方法能够将目的基因成功转导至耳蜗组织并表达。展开更多
基金supported by grants from the Yunnan Provincial Government(2013FA0512011HA005)+1 种基金the National Science Foundation of China(NSFC 81471966 to JZ and NSFC 31200964 to YX)the common project of the Panzhihua Science and Technology Bureau from China(2012CY-S-22(9)to HH)
文摘Herpes simplex virus type 1（HSV-1） enters productive infection after infecting epithelial cells, where it controls the host nucleus to make viral proteins, starts viral DNA synthesis and assembles infectious virions. In this process, replicating viral genomes are organized into replication centers to facilitate viral growth. HSV-1 is known to use host factors, including host chromatin and host transcription regulators, to transcribe its genes; however, the invading virus also encounters host defense and stress responses to inhibit viral growth. Recently, we found that HSV-1 replication centers recruit host factor CTCF but exclude γH2A.X. Thus, HSV-1 replication centers may selectively recruit cellular factors needed for viral growth, while excluding host factors that are deleterious for viral transcription or replication. Here we report that the viral replication centers selectively excluded modified histone H3, including heterochromatin mark H3K9me3, H3S10 P and active chromatin mark H3K4me3, but not unmodified H3. We found a dynamic association between the viral replication centers and host RNA polymerase II. The centers also recruited components of the DNA damage response pathway, including 53BP1, BRCA1 and host antiviral protein SP100. Importantly, we found that ATM kinase was needed for the recruitment of CTCF to the viral centers. These results suggest that the HSV-1 replication centers took advantage of host signaling pathways to actively recruit or exclude host factors to benefit viral growth.
文摘This thesis gives the overview of concept and constitution of the closed supply chain of green agricultural products based on logistics center,and the necessity of regarding logistics center as core enterprise.Meanwhile,it analyzes the function of logistics center of agricultural products,namely functions of exchange,collection,distribution,storage and transportation.It also poses the prerequisites of logistics center.The logistics center of agricultural products must have stable profit expectancy,prominent scale,strong appealing power,capacity of bearing market risk and basic thoughts of supply chain management.The functional design of the closed supply chain of green agricultural products has been discussed in 5 aspects,namely logistics center,production base,providers of means of production,retailer and consumer.The operating thoughts of the closed supply chain of green agricultural products based on logistics center are put forward on the basis of the research of operating objective and service target of supply chain as follows:first,based on local principal agricultural products,the logistics center mainly distributes the massed agricultural products;second,it is necessary to choose appropriate strategic cooperative partners to sign contract;third,the operating procedure of supply chain entails bringing in professional managerial talents of supply chain;finally,the relationships of supply chain should be maintained.
文摘一般来讲,if 是最普通的连接词,表示单纯的条件或假设。但是在某些特殊情况下,if 却有其特殊用法,请看下面的例句如何理解。The necklace costs ＄250 if it costs a cent.将 if 从句看作是单纯的条件句,显然是不合逻辑的,这种情况下,if 从句失去了其字面意义,有“敢肯定(敢断言)……至少……”之意,那么应翻译为“(我敢说)这条项链至少值250美元”。由此可见,if 从句并不单纯表示条件,而是在条件中寓有肯定主句主论的含义,即 if 从句的作用是:用能够类比的最小单位(如 a cent,a day,a pen-ny,an inch,an ounce,a man,once 等)来对比,强调主句中所要肯定的数字,
文摘随着计算机信息技术的发展,云计算与大数据技术的出现成为信息产业的重大变革。云计算、物联网、移动互联网的迅速发展催生大数据时代的到来,Hadoop分布式集群的成功搭建为大数据工程研究提供了基础环境。结合虚拟机,通过基于Cent OS 7的系统安装、网络配置、远程链接、集群搭建、免密登录、时间同步等过程的研究和实现,让Hadoop集群的配置更加方便、快捷。
文摘With the popularity of Computer Assisted Language Learning(CALL),autonomous language learning centers have been established in the universities throughout the country.However,there are many problems exist in the practice of the autonomous language learning center.This essay tries to discuss the problems and its countermeasures with an aim of improving College English learning and teaching by the example of autonomous language learning center in Nanyang Institute of Technology.
文摘首都国际会展中心项目作为超大型公共建筑工程,其特点表现于钢结构体量大,结构体系和节点构造复杂多变,构件数量多且单件重量大,合理选择施工方法和施工机械是重点。以首都国际会展中心为研究对象,以保证中心项目钢结构工程进度、质量为目的,通过BIM(Building Information Modeling)结合智能建造技术在钢结构生产安装全过程中的综合应用,有效降低了项目因质量问题拆改所带来的经济损失,确保了现场钢结构安装进度,节约钢结构生产过程中所带来的损耗,为类似工程提供了宝贵经验。
文摘The rapid growth of foreign investment in China will continue and may even accelerate after the country enters the World Trade Organization (WTO), analysts said. "More fields are opening to foreign investors and market regulations are becoming more transparent, which encourages foreign companies to increase their presence in China," said Sun Xiaohua, a senior researcher at the Chinese Academy of International Trade and
文摘面向园区综合能源系统中供能方与用能方的角色互换,以及园区低碳经济运行的强约束,提出了一种考虑动态参数的阶梯型碳交易机制和需求响应的园区级综合能源系统主从博弈优化调度方法。首先,将园区级综合能源系统中能源运营商(energy system operator,ESO)设定为上层领导者、综合能源系统园区设定为下层跟随者,并且能源运营商以最大化自身效益为目标,通过制定与园区间的购售电价格、碳交易基价、价格增长幅度,引导下层园区优化;下层园区以最小化其运行成本为目标,对上层发布的价格信息做出反应,从而构建主从博弈模型。其次,充分考虑园区级综合能源系统的低碳经济运行约束,在博弈模型中引入考虑动态参数的阶梯型碳交易机制以限制二氧化碳排放量,并在园区侧引入需求响应。最后,利用水母搜索算法对上层发布的购售电价、碳交易基价、价格增长幅度进行优化,利用CPLEX优化下层园区设备出力、需求响应以及购售电计划。仿真结果证明了所提模型和方法的有效性。
文摘目的研究腺病毒携带目的基因经小鼠耳后入路圆窗膜显微注射途径耳蜗转导的可行性,为以小鼠作为动物模型的内耳基因治疗提供实验基础和解剖学依据。方法12只C57BL/6J小鼠分为2组,实验组(8只)以重组腺病毒携带的增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP)、对照组(4只)以人工外淋巴液经耳后入路圆窗膜显微注射注入耳蜗内。分别于术后5、14天取双侧耳蜗标本做基底膜铺片,在激光共聚焦显微镜下观察GFP表达。结果术后动物存活10只(每组死亡1只)。实验组转染后耳蜗底回基底膜及螺旋神经节上目的基因有表达,14天组强于5天组。对照组耳蜗未见荧光表达。结论耳后入路操作简单、损伤小、易于暴露圆窗龛。耳后入路圆窗膜显微注射腺病毒携带目的基因转导的方法能够将目的基因成功转导至耳蜗组织并表达。
