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Isolation and Physiological Characterization of Synechococcus cedrorum 1191 Strain Tolerant to Heavy Metals and Pesticides 被引量:1
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作者 R.GOTHALWAL P.S.BISEN 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1993年第2期187-194,共8页
The toxicity of heavy metals (Hg2 + , Zn2 + ) and pesticides has been investigated by comparing the physiological properties in wild and tolerant strains of Synechococcus cedrorum 1191. The differential pattern of gro... The toxicity of heavy metals (Hg2 + , Zn2 + ) and pesticides has been investigated by comparing the physiological properties in wild and tolerant strains of Synechococcus cedrorum 1191. The differential pattern of growth, absorption spectra of pigments and nutrient uptake was observed in tolerant strain. 展开更多
关键词 Isolation and Physiological Characterization of Synechococcus cedrorum 1191 Strain Tolerant to Heavy Metals and Pesticides
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Adaptive Response of Wild and Mutant Type Synechococcus cedrorum to a Polychlorinated Pesticide-Endosulfan
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作者 P.S.BISEN SHRUTIMATHUR 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1993年第3期265-272,共8页
The effect of endosulfan, a hexachlorinated pesticide, on growth, inorganic nitrogenous nutrient uptake (NO3-, NO2- and NH4+), change in pigmentation and glycogen content on wild type and chemically mutagenised cells ... The effect of endosulfan, a hexachlorinated pesticide, on growth, inorganic nitrogenous nutrient uptake (NO3-, NO2- and NH4+), change in pigmentation and glycogen content on wild type and chemically mutagenised cells of Synechococcus cedrorum was investigated. The pattern of response to pesticide stress in wild and mutant type was the same. Growth reappeared in both after a period of initial lag in presence of endosulfan. The duration of lag increased with increasing doses of pesticide. Paradoxically, however, the rate of uptake of NO-3, NO-2 and NH+4 pigment and glycogen content progressively increased with increasing doses. The difference in the adaptation response between wild and mutant types was observed only in the concentration of pesticide that could be tolerated; with the mutant tolerating 2.5 fold more. 展开更多
关键词 Adaptive Response of Wild and Mutant Type Synechococcus cedrorum to a Polychlorinated Pesticide-Endosulfan
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雪松聚球藻对重金属镉的抗性和解毒作用 被引量:18
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作者 戴玲芬 高宏 夏建荣 《应用与环境生物学报》 CAS CSCD 1998年第2期192-195,共4页
培养液中不同浓度(c)的重金属镉(Cd)对雪松聚藻(Synechococcuscedrorum)有相当的致毒作用,当c(Cd)>2μmol/L时,藻体的生长和光合作用均明显受到抑制,c>10μmol/L时,藻细胞出现解体,光合放氧量几近0,然而,当按梯度逐... 培养液中不同浓度(c)的重金属镉(Cd)对雪松聚藻(Synechococcuscedrorum)有相当的致毒作用,当c(Cd)>2μmol/L时,藻体的生长和光合作用均明显受到抑制,c>10μmol/L时,藻细胞出现解体,光合放氧量几近0,然而,当按梯度逐级提高培养基中c(Cd)时,雪松聚球藻则相应产生对有毒金属Cd的抗性,在即使c(Cd)达20μmol/L的培养基中亦生长良好.为探讨该藻对重金属致毒的抗性产生机理及其解毒作用,对藻体蛋白和胞内Cd进行了分离检测,结果表明:Cd在进入藻细胞后,与胞内多肽结合形成一种大分子量的色素蛋白复合体,和一种低分子量的金属结合蛋白,特别是后一种蛋白在藻细胞中的含量明显受外界Cd的诱导,并起解毒作用. 展开更多
关键词 藻类 雪松聚球藻 Cd 金属结合蛋白 解毒
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雪松聚球藻金属硫蛋白的纯化及其性质的研究
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作者 夏建荣 戴玲芬 林惠民 《水生生物学报》 CAS CSCD 北大核心 1998年第2期112-118,共7页
在雪松聚球藻的培养基中逐渐增加氯化镉的浓度以诱导藻细胞内金属硫蛋白(NT)的合成,经SephedexG-50、DEAE-cellulose(DE-52)和SephadexG-25柱层析,获得的MT没有亚型,经SDS-PAGE分析是高度均一的。每个蛋白分子约含5个Cd原子... 在雪松聚球藻的培养基中逐渐增加氯化镉的浓度以诱导藻细胞内金属硫蛋白(NT)的合成,经SephedexG-50、DEAE-cellulose(DE-52)和SephadexG-25柱层析,获得的MT没有亚型,经SDS-PAGE分析是高度均一的。每个蛋白分子约含5个Cd原子,10个巯基,分子量约为7.6KD,等电点pH4、5左右,半胱氨酸含量约占总氨基酸量的15.5%,还含有少量的芳香族氨基酸,MT的最大紫外吸收在250nm左右,在低pH下稳定性较差。 展开更多
关键词 雪松聚球藻 金属硫蛋白 纯化 性质
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