为研究几丁质结合蛋白(Chitin-binding protein D,CbpD)对溶藻弧菌(Vibrio alginolyticus)毒力和相关生物学特性的影响,通过同源重组技术构建溶藻弧菌ZJ-T的cbpD基因缺失突变株ZJ-T-ΔcbpD,比较突变株与野生株对斑马鱼(Danio rerio)的毒...为研究几丁质结合蛋白(Chitin-binding protein D,CbpD)对溶藻弧菌(Vibrio alginolyticus)毒力和相关生物学特性的影响,通过同源重组技术构建溶藻弧菌ZJ-T的cbpD基因缺失突变株ZJ-T-ΔcbpD,比较突变株与野生株对斑马鱼(Danio rerio)的毒性,以及毒力相关生理过程,包括生长能力、运动性、胞外蛋白酶分泌活性、溶血活性、抗生素敏感性、生物膜形成、过氧化氢(H_(2)O_(2))和铜离子(Cu^(2+))抗性以及铁离子(Fe^(3+))吸收能力的差异。研究发现,cbpD缺失后,溶藻弧菌对斑马鱼的毒力显著减弱,且细菌游动能力、涌动能力和胞外蛋白酶活性均降低,突变株对Cu2+的抗性提高;cbpD的突变不影响溶藻弧菌在富营养培养基中的生长、生物膜的形成、溶血活性、对大部分抗生素敏感性、对H_(2)O_(2)的抗性和Fe3+的获取能力。结果表明,cbpD可能通过正调控溶藻弧菌的运动能力和胞外蛋白酶分泌活性,而促进溶藻弧菌的毒力。展开更多
In this study the mechanical and erosion wear behavior of bamboo fiber reinforce epoxy composites filled with Cement By-Pass Dust (CBPD) were studied. The effect of CBPD content and alkalization on the various propert...In this study the mechanical and erosion wear behavior of bamboo fiber reinforce epoxy composites filled with Cement By-Pass Dust (CBPD) were studied. The effect of CBPD content and alkalization on the various properties of these composites was also investigated. Taguchi’s orthogonal arrays are used for analysis of experiential results. It identifies significant control factors influencing the erosion wear and also outlines significant interaction effects. Analysis of variance (ANOVA) test has also been performed on the measured data to find the most significant factors affecting erosion rate. Finally, eroded surfaces of both untreated and alkali treated bamboo fiber reinforced composites were characterized using SEM.展开更多
Forests play a crucial role in regulating the carbon balance and maintaining global climate stability.Remote sensing has provided new perspectives for regional monitoring of vegetation phenology.However,an accurate me...Forests play a crucial role in regulating the carbon balance and maintaining global climate stability.Remote sensing has provided new perspectives for regional monitoring of vegetation phenology.However,an accurate method for extracting the photosynthetic phenology of forests remains challenging.This study proposes an innovative method,the change point estimation of forest photosynthetic phenology method based on the maximum perpendicular distance(CBPD).CBPD extracted the dates of the start of the season(SOS)and the end of the season(EOS)for forests in North America from solar-induced chlorophyll fluorescence and daily flux tower observations.The validation results of CBPD indicated that compared to those of the double-logistic,first-order derivative,and dynamic threshold methods,the root mean square error of CBPD decreased by 0.04 to 14.04 d,while Pearson’s correlation coefficient and agreement index increased by 0.03 to 0.30 and by 0.34 to 21.52,respectively.Furthermore,CBPD demonstrated substantial consistency(P<0.01)with cross-validation based on remote sensing of photosynthetic phenology.In addition,SOS exhibited greater interannual variability compared to EOS.SOS was dominated by air temperature in 93.89% of the forest area.EOS was dominated by radiation in 48.70% of the forest area.In summary,CBPD has a great potential for tracking forest photosynthetic phenology,offering crucial insights into phenological responses to climate variations.展开更多
文摘为研究几丁质结合蛋白(Chitin-binding protein D,CbpD)对溶藻弧菌(Vibrio alginolyticus)毒力和相关生物学特性的影响,通过同源重组技术构建溶藻弧菌ZJ-T的cbpD基因缺失突变株ZJ-T-ΔcbpD,比较突变株与野生株对斑马鱼(Danio rerio)的毒性,以及毒力相关生理过程,包括生长能力、运动性、胞外蛋白酶分泌活性、溶血活性、抗生素敏感性、生物膜形成、过氧化氢(H_(2)O_(2))和铜离子(Cu^(2+))抗性以及铁离子(Fe^(3+))吸收能力的差异。研究发现,cbpD缺失后,溶藻弧菌对斑马鱼的毒力显著减弱,且细菌游动能力、涌动能力和胞外蛋白酶活性均降低,突变株对Cu2+的抗性提高;cbpD的突变不影响溶藻弧菌在富营养培养基中的生长、生物膜的形成、溶血活性、对大部分抗生素敏感性、对H_(2)O_(2)的抗性和Fe3+的获取能力。结果表明,cbpD可能通过正调控溶藻弧菌的运动能力和胞外蛋白酶分泌活性,而促进溶藻弧菌的毒力。
文摘In this study the mechanical and erosion wear behavior of bamboo fiber reinforce epoxy composites filled with Cement By-Pass Dust (CBPD) were studied. The effect of CBPD content and alkalization on the various properties of these composites was also investigated. Taguchi’s orthogonal arrays are used for analysis of experiential results. It identifies significant control factors influencing the erosion wear and also outlines significant interaction effects. Analysis of variance (ANOVA) test has also been performed on the measured data to find the most significant factors affecting erosion rate. Finally, eroded surfaces of both untreated and alkali treated bamboo fiber reinforced composites were characterized using SEM.
基金supported in part by the Postdoctor Project of Hubei Province under Grant Number 2024HBBHCXA064the Natural Resources Science and Technology Innovation Projects in Fujian Province under Grant Number KY-030000-04-2024-033+4 种基金the Open Fund of the Key Laboratory of JiangHuai Arable Land Resources Protection and Ecorestoration under Grant Number ARPE-2024-KF01the National Natural Science Foundation of China under Grant Number 42090012the Sichuan Science and Technology Program under Grant Numbers 2022YFN0031,2023YFS0381,and 2023YFN0022the Inte rgovernmental International Science and Technology Inno vation Cooperation Project under Grant Number 2023YFE0110400the Key Technology and Application Demonstration for Integ rated Remote Sensing Monitoring of Safety in Key Projects under Grant Number 2023YFB3906100.
文摘Forests play a crucial role in regulating the carbon balance and maintaining global climate stability.Remote sensing has provided new perspectives for regional monitoring of vegetation phenology.However,an accurate method for extracting the photosynthetic phenology of forests remains challenging.This study proposes an innovative method,the change point estimation of forest photosynthetic phenology method based on the maximum perpendicular distance(CBPD).CBPD extracted the dates of the start of the season(SOS)and the end of the season(EOS)for forests in North America from solar-induced chlorophyll fluorescence and daily flux tower observations.The validation results of CBPD indicated that compared to those of the double-logistic,first-order derivative,and dynamic threshold methods,the root mean square error of CBPD decreased by 0.04 to 14.04 d,while Pearson’s correlation coefficient and agreement index increased by 0.03 to 0.30 and by 0.34 to 21.52,respectively.Furthermore,CBPD demonstrated substantial consistency(P<0.01)with cross-validation based on remote sensing of photosynthetic phenology.In addition,SOS exhibited greater interannual variability compared to EOS.SOS was dominated by air temperature in 93.89% of the forest area.EOS was dominated by radiation in 48.70% of the forest area.In summary,CBPD has a great potential for tracking forest photosynthetic phenology,offering crucial insights into phenological responses to climate variations.
