To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) wa...To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) was amplified from the recombinant plasmid pTG402 by using PCR technique and was subcloned into pUC118N and pUC119N. The single stranded recombinant phage DNA from the transformed E. coli MV1184 cells was used for sequencing. The sequence of xylE gene was proved to be the same as reported. The gene was then subcloned into the high expression plasmid pJLA503, its expression amount being about 34. 2% of the total bacterial proteins. Conclusion: xylE gene is highly expressed in host E. coli TG1.展开更多
Catechol adsorbed on TiO_(2)is one of the simplest models to explore the relevant properties of dye-sensitized solar cells.However,the effects of water and defects on the electronic levels and the excitonic properties...Catechol adsorbed on TiO_(2)is one of the simplest models to explore the relevant properties of dye-sensitized solar cells.However,the effects of water and defects on the electronic levels and the excitonic properties of the catechol/TiO_(2)interface have been rarely explored.Here,we investigate four catechol/TiO_(2)interfaces aiming to study the influence of coverage,water,and defects on the electronic levels and the excitonic properties of the catechol/TiO_(2)interface through the first-principles many-body Green’s function theory.We find that the adsorption of catechol on the rutile(110)surface increases the energies of both the TiO_(2)valence band maximum and conduction band minimum by approximately 0.7 eV.The increasing coverage and the presence of water can reduce the optical absorption of charge-transfer excitons with maximum oscillator strength.Regarding the reduced hydroxylated TiO_(2)substrate,the conduction band minimum decreases greatly,resulting in a sub-bandgap of 2.51 eV.The exciton distributions in the four investigated interfaces can spread across several unit cells,especially for the hydroxylated TiO2substrate.Although the hydroxylated TiO_(2)substrate leads to a lower open-circuit voltage,it may increase the separation between photogenerated electrons and holes and may therefore be beneficial for improving the photovoltaic efficiency by controlling its concentration.Our results may provide guidance for the design of highly efficient solar cells in future.展开更多
目的 观察早发型重度子痫前期患者血清中2-甲氧雌二醇(2-ME)水平和胎盘组织儿茶酚-O-甲基转移酶(COMT)蛋白表达变化及其临床意义。方法 选取孕周为28~34周早发型重度子痫前期单胎孕妇25例;随机选取同期健康对照组25例,标准为孕周小于34...目的 观察早发型重度子痫前期患者血清中2-甲氧雌二醇(2-ME)水平和胎盘组织儿茶酚-O-甲基转移酶(COMT)蛋白表达变化及其临床意义。方法 选取孕周为28~34周早发型重度子痫前期单胎孕妇25例;随机选取同期健康对照组25例,标准为孕周小于34周早产的单胎健康孕妇。收集两组年龄、孕周、孕产次、孕前体质量指数、采血日体质量指数、收缩压、舒张压、分娩孕周、期待治疗时间临床资料。采用酶联免疫吸附法检测两组孕妇血清2-ME水平,分析2-ME与临床指标的相关性。免疫组化检测两组胎盘组织中COMT的表达,逆转录-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western Blot)法分别检测胎盘组织中COMT mRNA和蛋白的表达。结果 早发型重度子痫前期(EOSPE)组血清2-ME水平为(525.3±172.6)pg/mL,健康对照(NP)组为(705.9±157.4)pg/mL,EOSPE组血清2-ME水平低于NP组,差异有统计学意义( P <0.05);2-ME水平与收缩压呈负相关( r =-0.648, P <0.05);与期待治疗时间呈正相关( r =0.694, P <0.05)。两组孕妇胎盘组织中均有COMT表达,EOSPE组COMT mRNA相对表达量(0.447±0.152)与NP组COMT mRNA相对表达量(0.467±0.151)比较,差异无统计学意义( P >0.05);EOSPE组胎盘组织中COMT 蛋白相对表达量(0.891 6±0.102 0)与NP组(0.924 0±0.158 7)比较差异无统计学意义( P >0.05)。结论 早发型重度子痫前期患者血清2-ME水平降低,并与病情严重程度相关;胎盘COMT表达无明显变化。提示2-ME水平降低可能会导致子痫前期发生及发展,但与胎盘COMT表达无关。展开更多
文摘To construct a high-level expression of xylE gene in E. coli for quick detection of environmental pollution of aromatic compounds. Methods and Results: XylE gene coding for the catechol 2, 3 dioxygenase (CatO2ase ) was amplified from the recombinant plasmid pTG402 by using PCR technique and was subcloned into pUC118N and pUC119N. The single stranded recombinant phage DNA from the transformed E. coli MV1184 cells was used for sequencing. The sequence of xylE gene was proved to be the same as reported. The gene was then subcloned into the high expression plasmid pJLA503, its expression amount being about 34. 2% of the total bacterial proteins. Conclusion: xylE gene is highly expressed in host E. coli TG1.
基金supported by the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(No.2020L0609 and No.2020L0556)the Doctoral research funds of Jinzhong University(jzxybsjjxm2019005)the Basic Research Program in Shanxi Province under the Grant No.20210302124345。
文摘Catechol adsorbed on TiO_(2)is one of the simplest models to explore the relevant properties of dye-sensitized solar cells.However,the effects of water and defects on the electronic levels and the excitonic properties of the catechol/TiO_(2)interface have been rarely explored.Here,we investigate four catechol/TiO_(2)interfaces aiming to study the influence of coverage,water,and defects on the electronic levels and the excitonic properties of the catechol/TiO_(2)interface through the first-principles many-body Green’s function theory.We find that the adsorption of catechol on the rutile(110)surface increases the energies of both the TiO_(2)valence band maximum and conduction band minimum by approximately 0.7 eV.The increasing coverage and the presence of water can reduce the optical absorption of charge-transfer excitons with maximum oscillator strength.Regarding the reduced hydroxylated TiO_(2)substrate,the conduction band minimum decreases greatly,resulting in a sub-bandgap of 2.51 eV.The exciton distributions in the four investigated interfaces can spread across several unit cells,especially for the hydroxylated TiO2substrate.Although the hydroxylated TiO_(2)substrate leads to a lower open-circuit voltage,it may increase the separation between photogenerated electrons and holes and may therefore be beneficial for improving the photovoltaic efficiency by controlling its concentration.Our results may provide guidance for the design of highly efficient solar cells in future.
文摘目的 观察早发型重度子痫前期患者血清中2-甲氧雌二醇(2-ME)水平和胎盘组织儿茶酚-O-甲基转移酶(COMT)蛋白表达变化及其临床意义。方法 选取孕周为28~34周早发型重度子痫前期单胎孕妇25例;随机选取同期健康对照组25例,标准为孕周小于34周早产的单胎健康孕妇。收集两组年龄、孕周、孕产次、孕前体质量指数、采血日体质量指数、收缩压、舒张压、分娩孕周、期待治疗时间临床资料。采用酶联免疫吸附法检测两组孕妇血清2-ME水平,分析2-ME与临床指标的相关性。免疫组化检测两组胎盘组织中COMT的表达,逆转录-聚合酶链反应(RT-PCR)和蛋白质印迹法(Western Blot)法分别检测胎盘组织中COMT mRNA和蛋白的表达。结果 早发型重度子痫前期(EOSPE)组血清2-ME水平为(525.3±172.6)pg/mL,健康对照(NP)组为(705.9±157.4)pg/mL,EOSPE组血清2-ME水平低于NP组,差异有统计学意义( P <0.05);2-ME水平与收缩压呈负相关( r =-0.648, P <0.05);与期待治疗时间呈正相关( r =0.694, P <0.05)。两组孕妇胎盘组织中均有COMT表达,EOSPE组COMT mRNA相对表达量(0.447±0.152)与NP组COMT mRNA相对表达量(0.467±0.151)比较,差异无统计学意义( P >0.05);EOSPE组胎盘组织中COMT 蛋白相对表达量(0.891 6±0.102 0)与NP组(0.924 0±0.158 7)比较差异无统计学意义( P >0.05)。结论 早发型重度子痫前期患者血清2-ME水平降低,并与病情严重程度相关;胎盘COMT表达无明显变化。提示2-ME水平降低可能会导致子痫前期发生及发展,但与胎盘COMT表达无关。
