With the aid of multi-agent based modeling approach to complex systems, the hierarchy simulation models of carrier-based aircraft catapult launch are developed. Ocean, carrier, aircraft, and atmosphere are treated as ...With the aid of multi-agent based modeling approach to complex systems, the hierarchy simulation models of carrier-based aircraft catapult launch are developed. Ocean, carrier, aircraft, and atmosphere are treated as aggregation agents, the detailed components like catapult, landing gears, and disturbances are considered as meta-agents, which belong to their aggregation agent. Thus, the model with two layers is formed i.e. the aggregation agent layer and the meta-agent layer. The information communication among all agents is described. The meta-agents within one aggregation agent communicate with each other directly by information sharing, but the meta-agents, which belong to different aggregation agents exchange their information through the aggregation layer first, and then perceive it from the sharing environment, that is the aggregation agent. Thus, not only the hierarchy model is built, but also the environment perceived by each agent is specified. Meanwhile, the problem of balancing the independency of agent and the resource consumption brought by real-time communication within multi-agent system （MAS） is resolved. Each agent involved in carrier-based aircraft catapult launch is depicted, with considering the interaction within disturbed atmospheric environment and multiple motion bodies including carrier, aircraft, and landing gears. The models of reactive agents among them are derived based on tensors, and the perceived messages and inner frameworks of each agent are characterized. Finally, some results of a simulation instance are given. The simulation and modeling of dynamic system based on multi-agent system is of benefit to express physical concepts and logical hierarchy clearly and precisely. The system model can easily draw in kinds of other agents to achieve a precise simulation of more complex system. This modeling technique makes the complex integral dynamic equations of multibodies decompose into parallel operations of single agent, and it is convenient to expand, maintain, and reuse the program codes.展开更多
AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A...AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT.展开更多
Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and...Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.展开更多
为解决遥感相机在运动过程中的抖动造成的图像位置偏移问题,提出了一种实时图像校正算法。由于在FPGA中采用HDL进行算法设计难度大、开发周期长,故设计中采用了C语言进行算法设计,然后借助Calypto公司的Catapult C Synthesis工具将抽象...为解决遥感相机在运动过程中的抖动造成的图像位置偏移问题,提出了一种实时图像校正算法。由于在FPGA中采用HDL进行算法设计难度大、开发周期长,故设计中采用了C语言进行算法设计,然后借助Calypto公司的Catapult C Synthesis工具将抽象的C设计转换成硬件RTL代码。在Catapult C Synthesis中对设计的算法进行了C/C++、RLT协同仿真测试,并在Xilinx XC5VLX110T型FPGA上进行了验证。仿真测试及硬件验证结果表明,采用Catapult C Synthesis设计的算法在时序、性能方面均满足设计要求,能够对偏移的图像进行实时校正。展开更多
To efficiently and fully utilize aircraft carrier resources,an optimization model is presented to deal with parameter matching between aircraft and carrier in the process of aircraft catapult launch.Based on carrier a...To efficiently and fully utilize aircraft carrier resources,an optimization model is presented to deal with parameter matching between aircraft and carrier in the process of aircraft catapult launch.Based on carrier aircraft longitudinal dynamic equations and theorem of kinetic energy in catapult launch course,the work characteristics of different forces are learned and a theory model of parameter matching is deduced.In view of the uncertainty of the model parameters of the theory model and the low matching accuracy of the approximate model,an optimization model of parameter matching is introduced in line with the structure of theory model and the approximate model and is generated by the proposed immune genetic algorithm.Compared with the original genetic algorithm and immune algorithm,the proposed algorithm has better calculation accuracy and convergence.The calculation results show that the optimization model occupies certain application value of engineering estimation from the comparison with the relevant literature data and has higher precision than the approximate models.The validity of the proposed approach is verified with numerical case study on a carrier based aircraft.展开更多
In order to enhance the safety of the catapult launch of the carrier-based aircraft,the catapult launch multibody dynamic model is established aiming at the problem of off center catapult launch.The whole catapult pro...In order to enhance the safety of the catapult launch of the carrier-based aircraft,the catapult launch multibody dynamic model is established aiming at the problem of off center catapult launch.The whole catapult process including four stages which are buffering,tensioning,releasing and taxiing is taken into consideration and the body dynamics of the off-center catapult during each stage is analyzed.The catapult launch dynamic differences between the conditions only considering taxiing and that considering four stages are compared,and the effects of the different initial off center distances considering four stages on the attitude,landing gear load and acceleration of the carrier based aircraft during catapult launch are discussed.The results show that only considering taxiing may underestimate the dynamics of the carrier-based aircraft substantially.When taking four stages into consideration,the initial off-center distance has small influence on the aircraft dynamic characteristics during buffering and tensioning but has larger influence on that during releasing and taxiing.The increase of the off-center distance will cause the enhancement of the aircraft rolling and yawing,which may lead to the load difference between the left and right landing gears and the increase of the aircraft lateral acceleration.The establishment and simulation of the catapult launch multi body dynamic model founded on buffering,tensioning,releasing and taxiing provide reference for the carrier-based aircraft design and analysis of the catapult launch dynamics.展开更多
Slingshot spiders use their conical webs to launch themselves at high speeds to catch prey.We built a launching mechanism inspired by the slingshot spider.Our launch mechanisms comprise a steel structure with eye bolt...Slingshot spiders use their conical webs to launch themselves at high speeds to catch prey.We built a launching mechanism inspired by the slingshot spider.Our launch mechanisms comprise a steel structure with eye bolts to hold a conical web made from latex rubber tubing.We pull the web by a winch using a lever arm.A quick-release mechanism connects the web to the winch using a tough-grid paracord.When we release the quick-return mechanism,the projectile material consisting of a steel ball achieved an acceleration of 5107 m s^(-2) or 521 times gravity,and a speed of 66.41 m s^(-1).The power per unit mass and energy per unit mass of our prototype was one tenth and one fifth that of the spider’s web respectively.In particular,we found that web structure consisting multiple radial web elements reinforced with circumferential web elements provide enhanced stiffness;as a comparison our web had a stiffness of about eight times that of traditional slingshot with a single radial web element.Our results suggest that adapting designs from nature can lead to potentially superior performance of man-made contraptions.展开更多
The architecture strategy of the Unmanned Aerial Vehicle(UAV)pneumatic launch system should continue to evolve to adapt to complex and variable operating environments.Architecture representation,decomposition perspect...The architecture strategy of the Unmanned Aerial Vehicle(UAV)pneumatic launch system should continue to evolve to adapt to complex and variable operating environments.Architecture representation,decomposition perspective,and cluster analysis play a vital role in the early phase of system architecture development.In order for the system to emerge anticipated and desirable intrinsic functional properties,an architecture decomposition method based on the ObjectProcess Methodology(OPM)and Design Structure Matrix(DSM)is put forward in this paper.The OPM is proposed to model the UAV launch process formally,and the matrix representation of the architecture of the pneumatic launch system is established.After the extension of the definition and operations of DSM,with the Idicula-Gutierrez-Thebeau Algorithm plus(IGTA+)clustering algorithm,the transformation of the pneumatic launch system architecture from process decomposition to function decomposition is demonstrated in this paper.The analysis shows that the architecture decomposition of the pneumatic launch system meets the functional requirements of stakeholders.展开更多
基金Aeronautical Science Foundation of China (2006ZA51004)
文摘With the aid of multi-agent based modeling approach to complex systems, the hierarchy simulation models of carrier-based aircraft catapult launch are developed. Ocean, carrier, aircraft, and atmosphere are treated as aggregation agents, the detailed components like catapult, landing gears, and disturbances are considered as meta-agents, which belong to their aggregation agent. Thus, the model with two layers is formed i.e. the aggregation agent layer and the meta-agent layer. The information communication among all agents is described. The meta-agents within one aggregation agent communicate with each other directly by information sharing, but the meta-agents, which belong to different aggregation agents exchange their information through the aggregation layer first, and then perceive it from the sharing environment, that is the aggregation agent. Thus, not only the hierarchy model is built, but also the environment perceived by each agent is specified. Meanwhile, the problem of balancing the independency of agent and the resource consumption brought by real-time communication within multi-agent system （MAS） is resolved. Each agent involved in carrier-based aircraft catapult launch is depicted, with considering the interaction within disturbed atmospheric environment and multiple motion bodies including carrier, aircraft, and landing gears. The models of reactive agents among them are derived based on tensors, and the perceived messages and inner frameworks of each agent are characterized. Finally, some results of a simulation instance are given. The simulation and modeling of dynamic system based on multi-agent system is of benefit to express physical concepts and logical hierarchy clearly and precisely. The system model can easily draw in kinds of other agents to achieve a precise simulation of more complex system. This modeling technique makes the complex integral dynamic equations of multibodies decompose into parallel operations of single agent, and it is convenient to expand, maintain, and reuse the program codes.
基金Supported by Grant G.0061.08 from the Fund for Scientific Research Flanders
文摘AIM: To investigate cellular 5-HT4(-h/+h) receptor distribution, particularly in the epithelial layer, by laser mi-crodissection and polymerase chain reaction (PCR) in porcine gastrointestinal (GI) tissues. METHODS: A stepwise approach was used to evaluate RNA quality and to study cell-specific 5-HT4 receptor mRNA expression in the porcine gastric fundus and colon descendens. After freezing, staining and laser microdissection and pressure catapulting (LMPC), RNA quality was evaluated by the Experion automated electrophoresis system. 5-HT4 receptor and glyceral-dehyde-3-phosphate dehydrogenase (GAPDH) expressions were examined by endpoint reverse transcription (RT)-PCR in mucosal and muscle-myenteric plexus (MMP) tissue fractions, in mucosal and MMP parts of hematoxylin and eosin (HE) stained tissue sections andin microdissected patches of the epithelial and circular smooth muscle cell layer in these sections. Pig gastric fundus tissue sections were also stained immunohisto-chemically (IHC) for enterochromaffin cells (EC cells; MAB352); these cells were isolated by LMPC and examined by endpoint RT-PCR. RESULTS: After HE staining, the epithelial and circular smooth muscle cell layer of pig colon descendens and the epithelial cell layer of gastric fundus were identified morphologically and isolated by LMPC. EC cells of pig gastric fundus were successfully stained by IHC and isolated by LMPC. Freezing, HE and IHC staining, and LMPC had no influence on RNA quality. 5-HT4 recep-tor and GAPDH mRNA expressions were detected in mucosa and MMP tissue fractions, and in mucosal and MMP parts of HE stained tissue sections of pig colon descendens and gastric fundus. In the mucosa tissue fractions of both GI regions, the expression of h-exon containing receptor [5-HT4(+h) receptor] mRNA was significantly higher (P<0.01) compared to 5-HT4(-h) re-ceptor expression, and a similar trend was obtained in the mucosal part of HE stained tissue sections. Large microdissected patches of the epithelial and circular smooth muscle cell layer of pig colon descendens and of the epithelial cell layer of pig gastric fundus, also showed 5-HT4 receptor and GAPDH mRNA expression. No 5-HT4 receptor mRNA expression was detected in gastric LMPC-isolated EC cells from IHC stained tissues, which cells were positive for GAPDH. CONCLUSION: Porcine GI mucosa predominantly expresses 5-HT4(+h) receptor splice variants, suggesting their contribution to the 5-HT4 receptor-mediated mu-cosal effects of 5-HT.
文摘Objective To compare the quality and quantity of total RNA from different source-original neurons applied in LMPC technique. Methods （ 1 ） Aglient 2100 bioanalyzer and RT-PCR were used to check the concentration and fragmentation of total RNA from unfixed, temporal fixed and fixed 12 h hypothalamus sections; （2）Different neurons of PVN and SON were collected by LMPC, CRH, TRH, AVP, OT mRNA level were measured by RT-PCR; （3）Labeled neurons by injecting CTB into stomach and non-labeled neurons in DMV collected by LMPC were checked for house keeping genes by RT-PCR. Results （ 1 ） Unfixed section had higher concentration and better quality of total RNA compared with fixed sections applied in LMPC ; relative short amplicons such as GAPDH, NSE, MCH and MCAR were successfully obtained from fixed and unfixed and long amplicon of GR can only be obtained from unfixed material; （2） In magnocellular PVN and SON the expressions of AVP and OT were more special than those in the parvocellular PVN. Oppositely, the expressions of CRH, TRH in the parvocellular were more special than the other two ; （3） The expressions of house keeping genes had no significant difference between labeled and non-labeled DMV neurons. Conclusion The quality and quantity of total RNA from unfixed brain tissues were better than fixed tissues applied in LMPC and the CTB tracer which may differentiate neurons had no significant effect on physiology of the neurons applied in LMPC. The results showed that the LMPC technique is suitable for the qualitative and quantitative study on individual neurons at mRNA level.
文摘为解决遥感相机在运动过程中的抖动造成的图像位置偏移问题,提出了一种实时图像校正算法。由于在FPGA中采用HDL进行算法设计难度大、开发周期长,故设计中采用了C语言进行算法设计,然后借助Calypto公司的Catapult C Synthesis工具将抽象的C设计转换成硬件RTL代码。在Catapult C Synthesis中对设计的算法进行了C/C++、RLT协同仿真测试,并在Xilinx XC5VLX110T型FPGA上进行了验证。仿真测试及硬件验证结果表明,采用Catapult C Synthesis设计的算法在时序、性能方面均满足设计要求,能够对偏移的图像进行实时校正。
基金supported by the Excellence Foundation of BUAA for Ph D.and the National Natural Science Foundation of China(No.91641123).
文摘To efficiently and fully utilize aircraft carrier resources,an optimization model is presented to deal with parameter matching between aircraft and carrier in the process of aircraft catapult launch.Based on carrier aircraft longitudinal dynamic equations and theorem of kinetic energy in catapult launch course,the work characteristics of different forces are learned and a theory model of parameter matching is deduced.In view of the uncertainty of the model parameters of the theory model and the low matching accuracy of the approximate model,an optimization model of parameter matching is introduced in line with the structure of theory model and the approximate model and is generated by the proposed immune genetic algorithm.Compared with the original genetic algorithm and immune algorithm,the proposed algorithm has better calculation accuracy and convergence.The calculation results show that the optimization model occupies certain application value of engineering estimation from the comparison with the relevant literature data and has higher precision than the approximate models.The validity of the proposed approach is verified with numerical case study on a carrier based aircraft.
文摘In order to enhance the safety of the catapult launch of the carrier-based aircraft,the catapult launch multibody dynamic model is established aiming at the problem of off center catapult launch.The whole catapult process including four stages which are buffering,tensioning,releasing and taxiing is taken into consideration and the body dynamics of the off-center catapult during each stage is analyzed.The catapult launch dynamic differences between the conditions only considering taxiing and that considering four stages are compared,and the effects of the different initial off center distances considering four stages on the attitude,landing gear load and acceleration of the carrier based aircraft during catapult launch are discussed.The results show that only considering taxiing may underestimate the dynamics of the carrier-based aircraft substantially.When taking four stages into consideration,the initial off-center distance has small influence on the aircraft dynamic characteristics during buffering and tensioning but has larger influence on that during releasing and taxiing.The increase of the off-center distance will cause the enhancement of the aircraft rolling and yawing,which may lead to the load difference between the left and right landing gears and the increase of the aircraft lateral acceleration.The establishment and simulation of the catapult launch multi body dynamic model founded on buffering,tensioning,releasing and taxiing provide reference for the carrier-based aircraft design and analysis of the catapult launch dynamics.
文摘Slingshot spiders use their conical webs to launch themselves at high speeds to catch prey.We built a launching mechanism inspired by the slingshot spider.Our launch mechanisms comprise a steel structure with eye bolts to hold a conical web made from latex rubber tubing.We pull the web by a winch using a lever arm.A quick-release mechanism connects the web to the winch using a tough-grid paracord.When we release the quick-return mechanism,the projectile material consisting of a steel ball achieved an acceleration of 5107 m s^(-2) or 521 times gravity,and a speed of 66.41 m s^(-1).The power per unit mass and energy per unit mass of our prototype was one tenth and one fifth that of the spider’s web respectively.In particular,we found that web structure consisting multiple radial web elements reinforced with circumferential web elements provide enhanced stiffness;as a comparison our web had a stiffness of about eight times that of traditional slingshot with a single radial web element.Our results suggest that adapting designs from nature can lead to potentially superior performance of man-made contraptions.
基金was co-supported by the National Defense Outstanding Youth Science Foundation,China(No.2018-JCJQZQ-053)the Natural Science Foundation of Jiangsu Province,China(No.BK20220911).
文摘The architecture strategy of the Unmanned Aerial Vehicle(UAV)pneumatic launch system should continue to evolve to adapt to complex and variable operating environments.Architecture representation,decomposition perspective,and cluster analysis play a vital role in the early phase of system architecture development.In order for the system to emerge anticipated and desirable intrinsic functional properties,an architecture decomposition method based on the ObjectProcess Methodology(OPM)and Design Structure Matrix(DSM)is put forward in this paper.The OPM is proposed to model the UAV launch process formally,and the matrix representation of the architecture of the pneumatic launch system is established.After the extension of the definition and operations of DSM,with the Idicula-Gutierrez-Thebeau Algorithm plus(IGTA+)clustering algorithm,the transformation of the pneumatic launch system architecture from process decomposition to function decomposition is demonstrated in this paper.The analysis shows that the architecture decomposition of the pneumatic launch system meets the functional requirements of stakeholders.
