AIM： To describe an acute extraocular muscle injury model in cats. METHODS： Seventy-two cats were randomly divided into 6 groups（12 cats per group）. Cats＇ left lateral recti were clamped using a surgical needle h...AIM： To describe an acute extraocular muscle injury model in cats. METHODS： Seventy-two cats were randomly divided into 6 groups（12 cats per group）. Cats＇ left lateral recti were clamped using a surgical needle holder with a clamping strength of 2（Groups A and D）, 4（Groups B and E） and 6 kg（Groups C and F）. The right lateral recti were treated as controls. On the 4^（th） and 7^（th） days, hematoxylin eosin（HE） staining, immunohistochemical staining for proliferating cell nuclear antigen（PCNA）, muscle force measurements and ocular alignment changes were performed to evaluate the extent of injuries. RESULTS： The morphological changes were graded as mild, moderate or severe by HE staining in all experiment groups. PCNA immunohistochemical staining indicated repairment of muscle fibers in the damaged area. On the 4^（th） and 7^（th） days after clamping, the injured lateral muscle exhibited an elevated threshold for electric stimulation. The muscle forces among groups 2, 4 and 6 kg injury at 4 d（Groups A, B and C） were statistically significant（P0.05）, respectively. In addition, medial deviation in ocular alignment was also present to various degrees in all groups. CONCLUSION： A cat model of acute extraocular muscle injury can be established by rectus clamping. Different clamping strengths can make different degrees of muscle injury. This model may help the future study in the acute extraocular muscle injury.展开更多
基金Supported by the Natural Science Research Foundation of Shaanxi Province,China(No.SJ08C241)
文摘AIM： To describe an acute extraocular muscle injury model in cats. METHODS： Seventy-two cats were randomly divided into 6 groups（12 cats per group）. Cats＇ left lateral recti were clamped using a surgical needle holder with a clamping strength of 2（Groups A and D）, 4（Groups B and E） and 6 kg（Groups C and F）. The right lateral recti were treated as controls. On the 4^（th） and 7^（th） days, hematoxylin eosin（HE） staining, immunohistochemical staining for proliferating cell nuclear antigen（PCNA）, muscle force measurements and ocular alignment changes were performed to evaluate the extent of injuries. RESULTS： The morphological changes were graded as mild, moderate or severe by HE staining in all experiment groups. PCNA immunohistochemical staining indicated repairment of muscle fibers in the damaged area. On the 4^（th） and 7^（th） days after clamping, the injured lateral muscle exhibited an elevated threshold for electric stimulation. The muscle forces among groups 2, 4 and 6 kg injury at 4 d（Groups A, B and C） were statistically significant（P0.05）, respectively. In addition, medial deviation in ocular alignment was also present to various degrees in all groups. CONCLUSION： A cat model of acute extraocular muscle injury can be established by rectus clamping. Different clamping strengths can make different degrees of muscle injury. This model may help the future study in the acute extraocular muscle injury.
