目的:构建条件性胰岛β细胞钙/钙调蛋白依赖性丝氨酸蛋白激酶(calcium/calmodulin-dependent serine protein kinase,CASK)基因敲除小鼠,为研究CASK基因在糖尿病发生中的机制提供动物模型。方法:将CASKloxp/-雌鼠与CASKloxp/Y雄鼠杂交,...目的:构建条件性胰岛β细胞钙/钙调蛋白依赖性丝氨酸蛋白激酶(calcium/calmodulin-dependent serine protein kinase,CASK)基因敲除小鼠,为研究CASK基因在糖尿病发生中的机制提供动物模型。方法:将CASKloxp/-雌鼠与CASKloxp/Y雄鼠杂交,获得基因型为CASKloxp/loxp雌鼠、CASKloxp/Y雄鼠;再让条件性胰岛β细胞特异性表达Cre重组酶雄鼠与CASKloxp/loxp雌鼠杂交获得CASKloxP/YMIP-Cre雄鼠和CASKloxP/-MIP-Cre雌鼠。CASKloxP/YMIP-Cre基因型小鼠即为本实验所需要构建的模型小鼠。小鼠生后1~2周剪尾,通过PCR鉴定小鼠基因型,4~5周龄时腹腔注射他莫昔芬诱导Cre重组酶表达后,利用实时荧光定量PCR、蛋白质印迹技术验证CASK基因敲除效果。结果:从引进这两种小鼠开始,繁殖10个月,共获得基因型为CASKloxP/YMIP-Cre的雄鼠28只,PCR结果证实小鼠基因型符合CASKloxP/YMIP-Cre。实时荧光定量PCR、蛋白质印迹结果显示CASKloxP/YMIP-Cre小鼠胰岛CASK表达量明显下降。结论:利用Cre/loxp系统,成功构建了条件性胰岛β细胞CASK基因敲除小鼠,为在动物水平研究CASK基因在糖尿病发病机制中的作用提供了研究平台。展开更多
Dry storage containers must be secured and reliable during long-term storage,and the effect of decay heat released from the internal spent fuel on the cask has become an important research topic.In this paper,a 3D com...Dry storage containers must be secured and reliable during long-term storage,and the effect of decay heat released from the internal spent fuel on the cask has become an important research topic.In this paper,a 3D computational fluid dynamics model is presented,and the accuracy of the calculation is verified,with computational errors of less than 6.2%.The thermal stress of the dry storage cask was estimated by coupling it with a transient temperature field.The total power remained constant and adjusting the power ratio of the inner and outer zones had a small effect on the stress results,with a maximum equivalent stress of approximately 5.2 kPa,which occurred at the lower edge of the shell.In the case of tilt,the temperature gradient varied in a wavy distribution,and the wave crest moved from right to left.Altering the tilt angle affects the air distribution in the annular gap,leading to the shell temperature being transformed,with a maximum equivalent stress of 202 MPa at the bottom of the shell.However,the equivalent stress in both cases was less than the yield stress(205 MPa).展开更多
Necessary adjustment ranges and accuracies of the pallet for ITER are presented. Detailed structural designs and structural finite element analyses for pallet components are made to determine whether the results satis...Necessary adjustment ranges and accuracies of the pallet for ITER are presented. Detailed structural designs and structural finite element analyses for pallet components are made to determine whether the results satisfy the requirements of the pallet structure to be used in ITER.展开更多
To aid the United States Nuclear Regulatory Commission,Sandia National Laboratories (SNL) was contracted to investigate the seismic behavior of typical dry cask storage systems. Parametric evaluations characterized th...To aid the United States Nuclear Regulatory Commission,Sandia National Laboratories (SNL) was contracted to investigate the seismic behavior of typical dry cask storage systems. Parametric evaluations characterized the sensitivity of calculated cask response characteristics to input parameters. The parametric evaluation investigated two generic cask designs (cylindrical and rectangular),three different foundation types (soft soil,hard soil,and rock),and three different casks to pad coefficients of friction (0.2,0.55,0.8) for earthquakes with peak ground accelerations of 0.25g,0.6g,1.0g and 1.25g. A total of 1 165 analyses were completed,with regression analyses being performed on the resulting cask response data to determine relationships relating the mean (16 % and 84 % confidence intervals on the mean) to peak ground acceleration,peak ground velocity,and pseudo-spectral acceleration at 1 Hz and 5 % damping. In general,the cylindrical casks experienced significantly larger responses in comparison to the rectangular cask. The cylindrical cask experienced larger top of cask displacements,larger cask rotations (rocking),and more occurrences of cask toppling (the rectangular cask never toppled over). The cylindrical cask was also susceptible to rolling once rocking had been initiated,a behavior not observed in the rectangular cask. Cask response was not overly sensitive to foundation type,but was significantly dependent on the response spectrum employed.展开更多
The Cask of Amontillado, which tells a story of revenge, is one of Edgar Allan Poe's masterpieces. The horrible and darksetting of the story, its theme of revenge, the description of the crazy character Fortunato ...The Cask of Amontillado, which tells a story of revenge, is one of Edgar Allan Poe's masterpieces. The horrible and darksetting of the story, its theme of revenge, the description of the crazy character Fortunato and the use of irony and symbols make it atypical Gothic fiction.展开更多
Edgar Allan Poe is a writer who establishes a new school in the American history of literature.He firstly and consciously takes the short story as an independent literature style,advances his own composing theory of s...Edgar Allan Poe is a writer who establishes a new school in the American history of literature.He firstly and consciously takes the short story as an independent literature style,advances his own composing theory of short stories and applies it to his composition of Gothic novels.He plays a great role in the contribution of the internal development of Gothic novels for he organically unified the moral exploration and psychological exploration,thoroughly displayed the conflict between the good and the evil as well as advanced the composing technique of Gothic novels to the extremely high artistic state.This paper holds that the Gothic technique is successfully applied in one of Edgar Allan Poe's famous short stories A Cask of Amontillado in three aspects: theme and plot,characterization and atmosphere,and its author reveals people's most deep-hidden inner world and exposes the ugliness of human's nature by way of the Gothic technique and the special effects of terror.展开更多
文摘目的:构建条件性胰岛β细胞钙/钙调蛋白依赖性丝氨酸蛋白激酶(calcium/calmodulin-dependent serine protein kinase,CASK)基因敲除小鼠,为研究CASK基因在糖尿病发生中的机制提供动物模型。方法:将CASKloxp/-雌鼠与CASKloxp/Y雄鼠杂交,获得基因型为CASKloxp/loxp雌鼠、CASKloxp/Y雄鼠;再让条件性胰岛β细胞特异性表达Cre重组酶雄鼠与CASKloxp/loxp雌鼠杂交获得CASKloxP/YMIP-Cre雄鼠和CASKloxP/-MIP-Cre雌鼠。CASKloxP/YMIP-Cre基因型小鼠即为本实验所需要构建的模型小鼠。小鼠生后1~2周剪尾,通过PCR鉴定小鼠基因型,4~5周龄时腹腔注射他莫昔芬诱导Cre重组酶表达后,利用实时荧光定量PCR、蛋白质印迹技术验证CASK基因敲除效果。结果:从引进这两种小鼠开始,繁殖10个月,共获得基因型为CASKloxP/YMIP-Cre的雄鼠28只,PCR结果证实小鼠基因型符合CASKloxP/YMIP-Cre。实时荧光定量PCR、蛋白质印迹结果显示CASKloxP/YMIP-Cre小鼠胰岛CASK表达量明显下降。结论:利用Cre/loxp系统,成功构建了条件性胰岛β细胞CASK基因敲除小鼠,为在动物水平研究CASK基因在糖尿病发病机制中的作用提供了研究平台。
基金the High-Performance Computing Center of Nanjing Tech University for supporting the computational resources
文摘Dry storage containers must be secured and reliable during long-term storage,and the effect of decay heat released from the internal spent fuel on the cask has become an important research topic.In this paper,a 3D computational fluid dynamics model is presented,and the accuracy of the calculation is verified,with computational errors of less than 6.2%.The thermal stress of the dry storage cask was estimated by coupling it with a transient temperature field.The total power remained constant and adjusting the power ratio of the inner and outer zones had a small effect on the stress results,with a maximum equivalent stress of approximately 5.2 kPa,which occurred at the lower edge of the shell.In the case of tilt,the temperature gradient varied in a wavy distribution,and the wave crest moved from right to left.Altering the tilt angle affects the air distribution in the annular gap,leading to the shell temperature being transformed,with a maximum equivalent stress of 202 MPa at the bottom of the shell.However,the equivalent stress in both cases was less than the yield stress(205 MPa).
文摘Necessary adjustment ranges and accuracies of the pallet for ITER are presented. Detailed structural designs and structural finite element analyses for pallet components are made to determine whether the results satisfy the requirements of the pallet structure to be used in ITER.
文摘To aid the United States Nuclear Regulatory Commission,Sandia National Laboratories (SNL) was contracted to investigate the seismic behavior of typical dry cask storage systems. Parametric evaluations characterized the sensitivity of calculated cask response characteristics to input parameters. The parametric evaluation investigated two generic cask designs (cylindrical and rectangular),three different foundation types (soft soil,hard soil,and rock),and three different casks to pad coefficients of friction (0.2,0.55,0.8) for earthquakes with peak ground accelerations of 0.25g,0.6g,1.0g and 1.25g. A total of 1 165 analyses were completed,with regression analyses being performed on the resulting cask response data to determine relationships relating the mean (16 % and 84 % confidence intervals on the mean) to peak ground acceleration,peak ground velocity,and pseudo-spectral acceleration at 1 Hz and 5 % damping. In general,the cylindrical casks experienced significantly larger responses in comparison to the rectangular cask. The cylindrical cask experienced larger top of cask displacements,larger cask rotations (rocking),and more occurrences of cask toppling (the rectangular cask never toppled over). The cylindrical cask was also susceptible to rolling once rocking had been initiated,a behavior not observed in the rectangular cask. Cask response was not overly sensitive to foundation type,but was significantly dependent on the response spectrum employed.
文摘The Cask of Amontillado, which tells a story of revenge, is one of Edgar Allan Poe's masterpieces. The horrible and darksetting of the story, its theme of revenge, the description of the crazy character Fortunato and the use of irony and symbols make it atypical Gothic fiction.
文摘Edgar Allan Poe is a writer who establishes a new school in the American history of literature.He firstly and consciously takes the short story as an independent literature style,advances his own composing theory of short stories and applies it to his composition of Gothic novels.He plays a great role in the contribution of the internal development of Gothic novels for he organically unified the moral exploration and psychological exploration,thoroughly displayed the conflict between the good and the evil as well as advanced the composing technique of Gothic novels to the extremely high artistic state.This paper holds that the Gothic technique is successfully applied in one of Edgar Allan Poe's famous short stories A Cask of Amontillado in three aspects: theme and plot,characterization and atmosphere,and its author reveals people's most deep-hidden inner world and exposes the ugliness of human's nature by way of the Gothic technique and the special effects of terror.
