Acyl-ACP thioesterases （FATs） terminates the fatty acid synthesis and allow the transport of fatty acids out of the plastids, which are the important determinants of cellular metabolism. FATB is a member of FAT enzy...Acyl-ACP thioesterases （FATs） terminates the fatty acid synthesis and allow the transport of fatty acids out of the plastids, which are the important determinants of cellular metabolism. FATB is a member of FAT enzymes that has been described previously in most of the plants. In silico cloning is a new method that utilizes the bioinformatics on the complete genome and available EST database. In this study, a full-length cDNA clone of PtFATB gene was isolated from Populus tomentosa using this approach. It is 1,450 bp in length and the open reading frame encodes a peptide of 421 amino acids. The predicted amino acid sequence shows significant homology with those from other plant species, which contain typical domains owned by FATB proteins. The transcripts of PtFATB were abundant in leaves, and less in roots detected by using semiquantitative RT-PCR. When the shoots were subjected to the stress treatments （cold, dry, NaC1） and ABA （Abscisic acid）, the expression of PtFATB was only slightly reduced under the treatment of low temperature. This suggests that the expression of PtFATB is in a constitutive fashion. This study provides the basis not only for the identification and characterization of this gene but also for the improvement of cold tolerance by controlling the expression of the PtFATB gene in trees in near future.展开更多
针对中国先进研究堆(CARR)的具体结构和运行特点,利用Fortran程序设计语言开发了CARR热工水力安全分析程序TSACC(Thermal-hydraulic and Safety Analysis Code for CARR)。TSACC完全采用模块化结构设计,便于二次开发,可应用于多种事故...针对中国先进研究堆(CARR)的具体结构和运行特点,利用Fortran程序设计语言开发了CARR热工水力安全分析程序TSACC(Thermal-hydraulic and Safety Analysis Code for CARR)。TSACC完全采用模块化结构设计,便于二次开发,可应用于多种事故工况及其他堆型的分析计算。基于程序验证的基本思想,分别利用TSACC和商用程序RELAP5/Mod3对CARR丧失厂外电源事故工况进行了计算。得到了堆芯平均通道以及最热通道内冷却剂流量、温度和最小偏离泡核沸腾比(MDNBR)等参数的瞬态响应。将TSACC计算结果与RELAP5/Mod3计算结果进行比较、分析后发现:除冷却剂发生倒流前后二者计算结果相差较大外,总体吻合较好。局部值差别较大的主要原因是两个程序在低流速区域选用的换热公式不同。程序验证结果表明了TSACC的准确性和适用性。展开更多
基金This work was supported by project "Regulation of Composition and Saturation of Fatty Acid in Trees by Genetic Engineering", Introduction of Foreign Advanced Agricultural Science and Technology into China (No. 2005-4-52).
文摘Acyl-ACP thioesterases （FATs） terminates the fatty acid synthesis and allow the transport of fatty acids out of the plastids, which are the important determinants of cellular metabolism. FATB is a member of FAT enzymes that has been described previously in most of the plants. In silico cloning is a new method that utilizes the bioinformatics on the complete genome and available EST database. In this study, a full-length cDNA clone of PtFATB gene was isolated from Populus tomentosa using this approach. It is 1,450 bp in length and the open reading frame encodes a peptide of 421 amino acids. The predicted amino acid sequence shows significant homology with those from other plant species, which contain typical domains owned by FATB proteins. The transcripts of PtFATB were abundant in leaves, and less in roots detected by using semiquantitative RT-PCR. When the shoots were subjected to the stress treatments （cold, dry, NaC1） and ABA （Abscisic acid）, the expression of PtFATB was only slightly reduced under the treatment of low temperature. This suggests that the expression of PtFATB is in a constitutive fashion. This study provides the basis not only for the identification and characterization of this gene but also for the improvement of cold tolerance by controlling the expression of the PtFATB gene in trees in near future.
文摘针对中国先进研究堆(CARR)的具体结构和运行特点,利用Fortran程序设计语言开发了CARR热工水力安全分析程序TSACC(Thermal-hydraulic and Safety Analysis Code for CARR)。TSACC完全采用模块化结构设计,便于二次开发,可应用于多种事故工况及其他堆型的分析计算。基于程序验证的基本思想,分别利用TSACC和商用程序RELAP5/Mod3对CARR丧失厂外电源事故工况进行了计算。得到了堆芯平均通道以及最热通道内冷却剂流量、温度和最小偏离泡核沸腾比(MDNBR)等参数的瞬态响应。将TSACC计算结果与RELAP5/Mod3计算结果进行比较、分析后发现:除冷却剂发生倒流前后二者计算结果相差较大外,总体吻合较好。局部值差别较大的主要原因是两个程序在低流速区域选用的换热公式不同。程序验证结果表明了TSACC的准确性和适用性。
