Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stituti...Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.展开更多
在常温静水条件下,研究了七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的急性毒性,并确定了各自对脊尾白虾的半致死浓度和安全浓度。结果表明,七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的96 h LC50依次为81.68、...在常温静水条件下,研究了七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的急性毒性,并确定了各自对脊尾白虾的半致死浓度和安全浓度。结果表明,七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的96 h LC50依次为81.68、71.81、51.72和39.06 mg/L,安全浓度依次为8.17、7.18、5.17和3.91 mg/L。4种化学品对脊尾白虾的毒性由强到弱依次为重铬酸钾>五水硫酸铜>高锰酸钾>七水硫酸锌。研究表明,脊尾白虾的死亡率与4种化学品浓度之间存在明显的剂量效应关系,4种化学品对脊尾白虾都有较强的毒性。展开更多
基金The National Basic Research Program of China (973 Program) under contract No. 2012CB114403General Program of National Natural Science Foundation of China to Dr. Fuhua Li under contract No. 31072203+1 种基金National Hightech Research and Development Program under contract Nos 2012AA10A404 and 2012AA092205China Agriculture Research System under contract No. CARS-47
文摘Recombinant bacteria secreting the transmembrane-truncated region of white spot syndrome virus （WSSV） envelope protein VP28 named DhpVB were constructed, using live Escherichia coli as a deliverer and a con- stitutive secretory expression plasmid as the expression vector. With the ability to deliver recombinant pro- tein products outside, DhpVB could make VP28 directly interact with the shrimp when injected into shrim- p. In order to test the protection potential, live DhpVB were injected into the marine shrimp Exopalamon carincauda Holthuis for three times and WSSV challenge was performed twice simultaneously at the last two injections of the bacteria. The results showed that DhpVB displayed statistically significant advantage over bacteria without VP28 after the second challenge with an average RI （Resistance index） of 0.67±0.08, compared with 0.41±0.09 of bacteria without VP28 （P 〈0.05）. The data suggested that a quasi-immune response may exist in E. carincauda and live bacteria carrying VP28 could enhance the shrimp resistance against WSSV.
文摘在常温静水条件下,研究了七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的急性毒性,并确定了各自对脊尾白虾的半致死浓度和安全浓度。结果表明,七水硫酸锌、高锰酸钾、五水硫酸铜和重铬酸钾对脊尾白虾的96 h LC50依次为81.68、71.81、51.72和39.06 mg/L,安全浓度依次为8.17、7.18、5.17和3.91 mg/L。4种化学品对脊尾白虾的毒性由强到弱依次为重铬酸钾>五水硫酸铜>高锰酸钾>七水硫酸锌。研究表明,脊尾白虾的死亡率与4种化学品浓度之间存在明显的剂量效应关系,4种化学品对脊尾白虾都有较强的毒性。
