The functionalization of living cells,both internally and externally,transforming them into microma-chines with specified functions,holds significant po-tential in fields such as biosensing,biocomputing,and intelligen...The functionalization of living cells,both internally and externally,transforming them into microma-chines with specified functions,holds significant po-tential in fields such as biosensing,biocomputing,and intelligent theranostics.However,due to the complexity and dynamic nature of living cells,it remains challenging to allocate exogenous function-al materials to specific locations within the cell or on its surface and maintain their positions stable for a reasonable period.Here,we devise a DNA-pro-grammed cargo distributing system(DCD),capable of distributing functional modules to the cell mem-brane or within the cell as needed.This system includes an amphiphilic DNA structure for determin-ing the destination of the cargo and a DNA connector carried on it for recognizing the DNA-encoded cargo.We test three different morphologies of amphiphilic DNA structures and find that their efficiencies in cell surface retention and cell internalization significantly varied,enabling the distribution of nanoparticle cargos on the cell membrane and within the cell in distinct proportions.Their positions can remain sta-ble for at least 6 h.Moreover,this allocation method shows specificity,which minimizes the deployment of mismatched cargo.This method provides new tools for the modular construction of cellular micro-machines.展开更多
基金supported by the National Key R&D Program of China(grant no.2020YFA0908900)the National Natural Science Foundation of China(grant nos.22105124,22325406,21934007,21991134,T2188102)+1 种基金2022 Shanghai“Science and Technology Innovation Action Plan”Fundamental Research Project(grant no.22JC1401203)the New Cornerstone Science Foundation,and the Open Research Fund of the National Facility for Translational Medicine(Shanghai,grant no.TMSK-2021-412).
文摘The functionalization of living cells,both internally and externally,transforming them into microma-chines with specified functions,holds significant po-tential in fields such as biosensing,biocomputing,and intelligent theranostics.However,due to the complexity and dynamic nature of living cells,it remains challenging to allocate exogenous function-al materials to specific locations within the cell or on its surface and maintain their positions stable for a reasonable period.Here,we devise a DNA-pro-grammed cargo distributing system(DCD),capable of distributing functional modules to the cell mem-brane or within the cell as needed.This system includes an amphiphilic DNA structure for determin-ing the destination of the cargo and a DNA connector carried on it for recognizing the DNA-encoded cargo.We test three different morphologies of amphiphilic DNA structures and find that their efficiencies in cell surface retention and cell internalization significantly varied,enabling the distribution of nanoparticle cargos on the cell membrane and within the cell in distinct proportions.Their positions can remain sta-ble for at least 6 h.Moreover,this allocation method shows specificity,which minimizes the deployment of mismatched cargo.This method provides new tools for the modular construction of cellular micro-machines.
