Traditional Palestinian medicine uses Thymus capitatus (T. capitatus), a plant recognized for its therapeutic properties due to its high concentration of essential oils such as thymol and carvacrol, to treat skin dise...Traditional Palestinian medicine uses Thymus capitatus (T. capitatus), a plant recognized for its therapeutic properties due to its high concentration of essential oils such as thymol and carvacrol, to treat skin diseases, gastrointestinal disorders, and respiratory infections. The present study was conducted to evaluate the antioxidant and anticancer activities of T. capitatus essential oil (EO). Moreover, this study employed computational methods including ADMET analysis and molecular docking. Using Gas chromatography-mass spectrometry (GC-MS) analysis, the phytochemical composition of T. capitatus essential oil was identified. The DPPH scavenging method was used to assess antioxidant activity. The Michigan Cancer Foundation-7 (MCF-7) and human colorectal carcinoma (HCT-116) cell lines were used to test for cytotoxic and cytostatic effects. The results of GC/MS analysis revealed 21 chemicals, accounting for 95.82% of their content, with carvacrol (61.23%), p-Cymene (9.49%) and γ-Terpinene (9.4%) being the most abundant. With an IC50 value of 0.27 ± 0.009 mg/mL, the DPPH assay demonstrated a robust scavenging capacity when compared to the IC50 value of butylated hydroxytoluene (BHT), which was 0.37 ± 0.007 mg/mL. T. capitatus EO showed potent anticancer activity on HCT-116 and MCF cell lines. The ADMET in-silico investigations revealed satisfying physicochemical and pharmacokinetics profiles, justified by good human intestinal absorption (HIA exceeding 93%), good permeabilities to the blood-brain barrier (BBB) and central nervous system (CNS), without any inhibition effect on 1A2, 2C9, 2C19, 2D6, and 3A4 cytochromes. Furthermore, all ligands were determined to be non-toxic, with no Ames mutagenicity detected based on toxicity predictions, making them ideal candidates for further drug development.展开更多
Invasive fungal infections have grown significantly over the last two decades, owing to an increase in immunocompromised hosts and geriatric patients. When the host’s defenses are compromised, such infections are ass...Invasive fungal infections have grown significantly over the last two decades, owing to an increase in immunocompromised hosts and geriatric patients. When the host’s defenses are compromised, such infections are associated with severe morbidity and mortality. Here, a rare case of fungal infection in a 61-year-old immunocompetent male patient from Saudi Arabia was reported, who suffered from pulmonary hemorrhage and Systemic Lupus Erythematous. Bronchoalveolar Lavage was used as a diagnostic tool to identify the fungus reported in the case. The pathogenic fungal specie identified as Magnusiomyces capitatus, in macroscopic and microscopic morphological characteristics of the colonies. Based on clinical evidence, liposomal amphotericin formulation was recommended for initial therapy against fungal infection. Also, liposomal amphotericin B induced mycological eradication up to 70 percent in patients with proven Magnusiomyces capitatus infection. In addition to addressing suspected Systemic lupus erythematosus, the patient’s health has improved with no evidence of pulmonary bleeding and hemoptysis.展开更多
Urban life has led to the creation of human-made environments that,from a microbiological perspective,provide extreme life conditions.Certain non-ubiquitous microorganisms such as thermophilic members of the black yea...Urban life has led to the creation of human-made environments that,from a microbiological perspective,provide extreme life conditions.Certain non-ubiquitous microorganisms such as thermophilic members of the black yeast genus Exophiala are enriched within these habitats for which no counterpart is known in nature.Dishwashers consistently accumulate a number of specific black,white and red yeasts on the rubber seals of doors and in stagnant water at the interior.Several of these yeasts are primarily known as agents of human opportunistic infections.In this review,the literature data are supported by a screening study involving 937 households in 15 cities in Turkey.Fungi were detected in 230 samples(24.5%).Using rDNA sequencing,the prevalent species were identified as Exophiala dermatitidis(n=116),Candida parapsilosis(n=44),E.phaeomuriformis(n=35),Magnusiomyces capitatus(n=22),Rhodotorula mucilaginosa(n=15),and C.lusitaniae(n=14).The possible role of dishwashers in transmitting disease is discussed.展开更多
文摘Traditional Palestinian medicine uses Thymus capitatus (T. capitatus), a plant recognized for its therapeutic properties due to its high concentration of essential oils such as thymol and carvacrol, to treat skin diseases, gastrointestinal disorders, and respiratory infections. The present study was conducted to evaluate the antioxidant and anticancer activities of T. capitatus essential oil (EO). Moreover, this study employed computational methods including ADMET analysis and molecular docking. Using Gas chromatography-mass spectrometry (GC-MS) analysis, the phytochemical composition of T. capitatus essential oil was identified. The DPPH scavenging method was used to assess antioxidant activity. The Michigan Cancer Foundation-7 (MCF-7) and human colorectal carcinoma (HCT-116) cell lines were used to test for cytotoxic and cytostatic effects. The results of GC/MS analysis revealed 21 chemicals, accounting for 95.82% of their content, with carvacrol (61.23%), p-Cymene (9.49%) and γ-Terpinene (9.4%) being the most abundant. With an IC50 value of 0.27 ± 0.009 mg/mL, the DPPH assay demonstrated a robust scavenging capacity when compared to the IC50 value of butylated hydroxytoluene (BHT), which was 0.37 ± 0.007 mg/mL. T. capitatus EO showed potent anticancer activity on HCT-116 and MCF cell lines. The ADMET in-silico investigations revealed satisfying physicochemical and pharmacokinetics profiles, justified by good human intestinal absorption (HIA exceeding 93%), good permeabilities to the blood-brain barrier (BBB) and central nervous system (CNS), without any inhibition effect on 1A2, 2C9, 2C19, 2D6, and 3A4 cytochromes. Furthermore, all ligands were determined to be non-toxic, with no Ames mutagenicity detected based on toxicity predictions, making them ideal candidates for further drug development.
文摘Invasive fungal infections have grown significantly over the last two decades, owing to an increase in immunocompromised hosts and geriatric patients. When the host’s defenses are compromised, such infections are associated with severe morbidity and mortality. Here, a rare case of fungal infection in a 61-year-old immunocompetent male patient from Saudi Arabia was reported, who suffered from pulmonary hemorrhage and Systemic Lupus Erythematous. Bronchoalveolar Lavage was used as a diagnostic tool to identify the fungus reported in the case. The pathogenic fungal specie identified as Magnusiomyces capitatus, in macroscopic and microscopic morphological characteristics of the colonies. Based on clinical evidence, liposomal amphotericin formulation was recommended for initial therapy against fungal infection. Also, liposomal amphotericin B induced mycological eradication up to 70 percent in patients with proven Magnusiomyces capitatus infection. In addition to addressing suspected Systemic lupus erythematosus, the patient’s health has improved with no evidence of pulmonary bleeding and hemoptysis.
文摘Urban life has led to the creation of human-made environments that,from a microbiological perspective,provide extreme life conditions.Certain non-ubiquitous microorganisms such as thermophilic members of the black yeast genus Exophiala are enriched within these habitats for which no counterpart is known in nature.Dishwashers consistently accumulate a number of specific black,white and red yeasts on the rubber seals of doors and in stagnant water at the interior.Several of these yeasts are primarily known as agents of human opportunistic infections.In this review,the literature data are supported by a screening study involving 937 households in 15 cities in Turkey.Fungi were detected in 230 samples(24.5%).Using rDNA sequencing,the prevalent species were identified as Exophiala dermatitidis(n=116),Candida parapsilosis(n=44),E.phaeomuriformis(n=35),Magnusiomyces capitatus(n=22),Rhodotorula mucilaginosa(n=15),and C.lusitaniae(n=14).The possible role of dishwashers in transmitting disease is discussed.
