Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in ...Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed展开更多
Cap蛋白是猪圆环病毒2型(porcine circovirus type 2,PCV2)的主要结构蛋白,能诱导免疫保护,是临床上利用血清学诊断PCV2的主要依据。本研究根据PCV2TJ株全基因核苷酸序列(GenBank登录号:KC751546)设计特异性引物,利用PCR从PCV2TJ株扩增...Cap蛋白是猪圆环病毒2型(porcine circovirus type 2,PCV2)的主要结构蛋白,能诱导免疫保护,是临床上利用血清学诊断PCV2的主要依据。本研究根据PCV2TJ株全基因核苷酸序列(GenBank登录号:KC751546)设计特异性引物,利用PCR从PCV2TJ株扩增获得Cap全基因,将该基因片段连接到原核表达载体pET-32a(+)中,获得重组质粒pET32a-Cap,经IPTG诱导获得约48ku的重组融合蛋白,Western blotting分析结果表明其与小鼠抗6×His单克隆抗体和PCV2阳性猪血清均呈阳性反应。本研究成功克隆Cap全基因,构建原核表达载体,并实现Cap融合蛋白的表达,这为PCV2Cap蛋白的功能研究及诊断方法的建立和亚单位疫苗的研制奠定了基础。展开更多
文摘Resistance to whitebacked planthopper(WBPH)in Chinese japonica riceChunjiang 06(CJ-06)was mediated by sucking inhibitory and ovicidal mecha-nism.The ovicidal reponse was a common self-defense mechanism againstWBPH in japonica.The ovicidal gene and its chromosomal position had alreadybeen identified.The sucking inhibitory nature of CJ-06 caused a definenon-preference behavior of WBPH in fields.A single dominant gene governed
文摘Cap蛋白是猪圆环病毒2型(porcine circovirus type 2,PCV2)的主要结构蛋白,能诱导免疫保护,是临床上利用血清学诊断PCV2的主要依据。本研究根据PCV2TJ株全基因核苷酸序列(GenBank登录号:KC751546)设计特异性引物,利用PCR从PCV2TJ株扩增获得Cap全基因,将该基因片段连接到原核表达载体pET-32a(+)中,获得重组质粒pET32a-Cap,经IPTG诱导获得约48ku的重组融合蛋白,Western blotting分析结果表明其与小鼠抗6×His单克隆抗体和PCV2阳性猪血清均呈阳性反应。本研究成功克隆Cap全基因,构建原核表达载体,并实现Cap融合蛋白的表达,这为PCV2Cap蛋白的功能研究及诊断方法的建立和亚单位疫苗的研制奠定了基础。
