The Gansu zokor(Eospalax cansus),a subterranean rodent endemic to the Loess Plateau of China,exhibits remarkable adaptability to hypoxic environments.While gut microbiota are known to regulate lipid metabolism through...The Gansu zokor(Eospalax cansus),a subterranean rodent endemic to the Loess Plateau of China,exhibits remarkable adaptability to hypoxic environments.While gut microbiota are known to regulate lipid metabolism through bile acid(BA)pathways,this phenomenon has not been investigated in subterranean rodents exposed to hypoxia.This study employed 16SrRNA sequencing,targeted analysis of BA metabolites in colonic contents,and assessments of BA and lipid metabolites alongside molecular analyses in the liver and ileum under conditions of acute and chronic hypoxia in Gansu zokors.The results revealed that hypoxia altered the composition of gut microbiota and BA pools in Gansu zokors.Hypoxia-induced changes increased the abundance of gut microbiota associated with BA metabolism,thereby modulating lipid metabolism via farnesoid X receptor(FXR)signaling in the distal ileum and liver cells.Under acute hypoxia,FXR upregulated lipid synthesis and suppressed fatty acidβ-oxidation by downregulating the carnitine palmitoyl-transferase1A(CPT1A)expression.Conversely,during chronic hypoxia,particularly under long-term exposure,FXR reduced lipid synthesis and enhanced fatty acidβ-oxidation by upregulating acyl-CoA oxidase(ACOX1)expression.In both hypoxic conditions,FXR facilitated lipoprotein metabolism.In summary,this study elucidates that gut microbiota-mediated BA metabolic pathways contribute to the Gansu zokor’s ability to maintain lipid metabolic homeostasis and adaptation to hypoxia.展开更多
To help resolve the systematic position of Myospalacine(zokors)and to clarify the phylogenetic relationships among zokor species,complete mitochondrial 12S rRNA and cytochrome b gene sequences were determined for seve...To help resolve the systematic position of Myospalacine(zokors)and to clarify the phylogenetic relationships among zokor species,complete mitochondrial 12S rRNA and cytochrome b gene sequences were determined for seven zokor species from China.Phylogenetic relationships among all extant zokors,except the Siberian zokor(Myospalax myospalax)and Smith’s zokor(Eospalax smithi),were reconstructed with the Chinese bamboo rat(Rhizomys sinensis)as an outgroup.Our results support the generic status of Eospalax and also support the validity of the specific status of Eospalax cansus,Eospalax baileyi and Eospalax rufescens.展开更多
基金supported by the Natural Science Basic Research Program of Shaanxi(Program No.2024JC-ZDXM-14).
文摘The Gansu zokor(Eospalax cansus),a subterranean rodent endemic to the Loess Plateau of China,exhibits remarkable adaptability to hypoxic environments.While gut microbiota are known to regulate lipid metabolism through bile acid(BA)pathways,this phenomenon has not been investigated in subterranean rodents exposed to hypoxia.This study employed 16SrRNA sequencing,targeted analysis of BA metabolites in colonic contents,and assessments of BA and lipid metabolites alongside molecular analyses in the liver and ileum under conditions of acute and chronic hypoxia in Gansu zokors.The results revealed that hypoxia altered the composition of gut microbiota and BA pools in Gansu zokors.Hypoxia-induced changes increased the abundance of gut microbiota associated with BA metabolism,thereby modulating lipid metabolism via farnesoid X receptor(FXR)signaling in the distal ileum and liver cells.Under acute hypoxia,FXR upregulated lipid synthesis and suppressed fatty acidβ-oxidation by downregulating the carnitine palmitoyl-transferase1A(CPT1A)expression.Conversely,during chronic hypoxia,particularly under long-term exposure,FXR reduced lipid synthesis and enhanced fatty acidβ-oxidation by upregulating acyl-CoA oxidase(ACOX1)expression.In both hypoxic conditions,FXR facilitated lipoprotein metabolism.In summary,this study elucidates that gut microbiota-mediated BA metabolic pathways contribute to the Gansu zokor’s ability to maintain lipid metabolic homeostasis and adaptation to hypoxia.
基金funded by the National Natural Science Foundation of China(No:30770256)the Key Projects(SZD 0420)of Sichuan Province.
文摘To help resolve the systematic position of Myospalacine(zokors)and to clarify the phylogenetic relationships among zokor species,complete mitochondrial 12S rRNA and cytochrome b gene sequences were determined for seven zokor species from China.Phylogenetic relationships among all extant zokors,except the Siberian zokor(Myospalax myospalax)and Smith’s zokor(Eospalax smithi),were reconstructed with the Chinese bamboo rat(Rhizomys sinensis)as an outgroup.Our results support the generic status of Eospalax and also support the validity of the specific status of Eospalax cansus,Eospalax baileyi and Eospalax rufescens.
