[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular mar...[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.展开更多
Objective:To determine? the anlihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde(MDA) level in the rat.Methods:Ke...Objective:To determine? the anlihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde(MDA) level in the rat.Methods:Kenaf seed oil(KSO),microencapsulated kenaf seed oil(MKSO),kenaf seed extract(KSE) and defatted kenaf seed meal(DKSM) were prepared and phytocHemicals screening on these samples were done prior in viro study.Phenolic compounds in KSF.were quantified using high performance liquid chromatography.There were 40(divided in eight diet groups of 5) male Sprague dawley rats adapted lo normal standard diet or hypercholesterolemic diet(HD) with or without the treatment of these kenaf samples for 32 days.Results:All the kenaf samples exhibited to contain most of the major phytocliemicals.KSE possessed gallic acid,tannic acid,catechin.benzaldehyde.benzoic acid,syringic acid,sinapic acid,ferulic acid,naringin acid,and protocatechuic acid.The significant higher(P<0.05) serum total cholesterol,low density lipoprotein cholesterol and MDA levels in HD group without treatment than the normal control group suggested the hypercholesterolemia was induced by the Incorporation of cholesterol into diet.KSE exhibited higher cholesterol-lowering properties due to the significant lower(P<0.05)in serum triglycerides,total cholesterol and MDA levels.KSF showed the highest efficiency of cholesterol-lowering activity,followed by KSO.MKSO and DKSM.Conclusions:DKSM.MKSO.KSO and KSE appeared lo have comparable anti-hypercholesterolemic effect with the commercial hypocholesterolemic drug.Hence,kenaf seed could be used as an alternative natural source lo replace the synthetic hypocholosterolemic drugs.展开更多
[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The...[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.展开更多
通过大田小区试验,研究减量灌溉和施肥对红麻(Hibiscus cannabinus)生长和产量的影响,以探讨红麻水肥利用特点,为红麻高产高效栽培水肥管理提供合理依据。结果表明,与足量施肥相比,减少25%施肥量显著降低红麻株高等农艺性状、生麻产量...通过大田小区试验,研究减量灌溉和施肥对红麻(Hibiscus cannabinus)生长和产量的影响,以探讨红麻水肥利用特点,为红麻高产高效栽培水肥管理提供合理依据。结果表明,与足量施肥相比,减少25%施肥量显著降低红麻株高等农艺性状、生麻产量和地上部产量;与足量灌溉相比,减少20%灌水量和40%灌水量均显著降低红麻株高等农艺性状和生麻产量,表明足量施肥和灌溉是红麻高产稳产的必备条件。足量施肥和灌溉的红麻生麻产量显著高于其他处理。减少25%施肥量时,减少20%灌水量的红麻生麻产量显著高于减少40%灌水量,但与足量灌溉之间的差异不明显,表明水和肥应协调供应。因此,为了获得理想的产量,红麻应按照灌水定额进行灌溉,施肥量采用200 kg N/hm2,66.7 kg P2O5/hm2,133.4 kg K2O/hm2。但水和肥有一种供应不足时,应当减少另一种的用量。展开更多
Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhe...Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhesive with the resin content of 4% for core particles and 50 g/m2 for bast fiber- woven sheets. The target board densities were set at 0.35, 0.45 and 0.55 g/cm3. The composite panels were evaluated with Japanese Industrial Standard for Particleboards (JIS A 5908- 2003).The results show that the composite panel has high modulus of rupture and internal bonding strength. The properties of 0.45 g/cm3 density composite panel are: MOR 20.4 MPa, MOE 1.94 MPa, IB 0.36 MPa, WA142%, TS 21%. Kenaf is a good raw material for making light-weight composite panels.展开更多
基金Supported by The National High Technology Research and Development Program of China(2001AA241211)Industry Special:Studyon the Efficient Production and Harvest Technique in Ramee, Flax,Jute/Kenaf(NYHYJX07-18)~~
文摘[Objective] This study was to reveal the genetic diversity and genetic relationship of the kenaf(Hibiscus cannabinus L.) resources from different origins, thus providing basis for genetic improvement and molecular marker-assisted breeding of kenaf. [Method] Ninety one ISSR molecular markers were used for amplification on 44 shares of kenaf germplasm resources, of which 21 showing good diversity and clear bands were chosen for PCR amplification. Based on amplification results, the genetic similarity coefficients among kenaf germplasm resources were calculated by using analytic software NTSYSpc-2.10e, and phylogenetic tree was then established via UPGMA. [Result] Totally 169 bands were amplified using the 21 screened primers, averagely 8.05 bands were amplified from each primer. Of them, 141 bands were polymorphic, accounting for 83.4%. When genetic similarity coefficient 0.887 was used as criterion L1, these 44 shares of kenaf germplasm could be classified to be 32 shares of cultivars and 12 shares of wild type or half-wild type varieties. When genetic similarity coefficient 0.897 was used as criterion L2, these 32 shares of cultivars could be further grouped into four sub-clusters. The genetic diversities between cultivars and wild type or half-wild type varieties were between 0.46-0.91, showing huge hereditary difference; while that among 32 cultivars were between 0.85-0.97, suggesting that genetic relationships among cultivars are relatively close and their genetic similarities are rather narrow. [Conclusion] ISSR could well determine the genetic similarities among kenaf germplasm resources and provide valuable molecular information for selecting parents of hybrid cross, which can lay a good foundation for DNA mapping of kenaf germplasm resources.
基金supported by the Ministry of Higher Education through the Fundamental Research Grant Scheme(FRGS/2/2010/SG/UCSI/03/1)
文摘Objective:To determine? the anlihypercholesterolemic effects of kenaf seed samples and compare with the commercial hypocholesterolemic drug on serum lipids profiles and malondialdehyde(MDA) level in the rat.Methods:Kenaf seed oil(KSO),microencapsulated kenaf seed oil(MKSO),kenaf seed extract(KSE) and defatted kenaf seed meal(DKSM) were prepared and phytocHemicals screening on these samples were done prior in viro study.Phenolic compounds in KSF.were quantified using high performance liquid chromatography.There were 40(divided in eight diet groups of 5) male Sprague dawley rats adapted lo normal standard diet or hypercholesterolemic diet(HD) with or without the treatment of these kenaf samples for 32 days.Results:All the kenaf samples exhibited to contain most of the major phytocliemicals.KSE possessed gallic acid,tannic acid,catechin.benzaldehyde.benzoic acid,syringic acid,sinapic acid,ferulic acid,naringin acid,and protocatechuic acid.The significant higher(P<0.05) serum total cholesterol,low density lipoprotein cholesterol and MDA levels in HD group without treatment than the normal control group suggested the hypercholesterolemia was induced by the Incorporation of cholesterol into diet.KSE exhibited higher cholesterol-lowering properties due to the significant lower(P<0.05)in serum triglycerides,total cholesterol and MDA levels.KSF showed the highest efficiency of cholesterol-lowering activity,followed by KSO.MKSO and DKSM.Conclusions:DKSM.MKSO.KSO and KSE appeared lo have comparable anti-hypercholesterolemic effect with the commercial hypocholesterolemic drug.Hence,kenaf seed could be used as an alternative natural source lo replace the synthetic hypocholosterolemic drugs.
文摘[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.
文摘通过大田小区试验,研究减量灌溉和施肥对红麻(Hibiscus cannabinus)生长和产量的影响,以探讨红麻水肥利用特点,为红麻高产高效栽培水肥管理提供合理依据。结果表明,与足量施肥相比,减少25%施肥量显著降低红麻株高等农艺性状、生麻产量和地上部产量;与足量灌溉相比,减少20%灌水量和40%灌水量均显著降低红麻株高等农艺性状和生麻产量,表明足量施肥和灌溉是红麻高产稳产的必备条件。足量施肥和灌溉的红麻生麻产量显著高于其他处理。减少25%施肥量时,减少20%灌水量的红麻生麻产量显著高于减少40%灌水量,但与足量灌溉之间的差异不明显,表明水和肥应协调供应。因此,为了获得理想的产量,红麻应按照灌水定额进行灌溉,施肥量采用200 kg N/hm2,66.7 kg P2O5/hm2,133.4 kg K2O/hm2。但水和肥有一种供应不足时,应当减少另一种的用量。
基金Supported by the talent fund of the Central South University of Forestry and Technology.
文摘Light-weight composite panels were manufactured using kenaf core particles as core material and kenaf bast fiber-woven sheets as top and bottom surfaces. Methylene diphenyldiisocyanate (MDI) resin was used as the adhesive with the resin content of 4% for core particles and 50 g/m2 for bast fiber- woven sheets. The target board densities were set at 0.35, 0.45 and 0.55 g/cm3. The composite panels were evaluated with Japanese Industrial Standard for Particleboards (JIS A 5908- 2003).The results show that the composite panel has high modulus of rupture and internal bonding strength. The properties of 0.45 g/cm3 density composite panel are: MOR 20.4 MPa, MOE 1.94 MPa, IB 0.36 MPa, WA142%, TS 21%. Kenaf is a good raw material for making light-weight composite panels.
