[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,a...[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,and the antitussive effects of different extraction fractions of A.caespitosa were observed.The inflammation models of acute inflammatory ear swelling in mice caused by xylene,granuloma in mice caused by cotton balls,and footpad swelling in mice caused by carrageenan were made,and the different extraction fractions were used to observe the effects of different extraction fractions on mouse ear swelling,granuloma and footpad swelling.And the levels of interleukin 6(IL-6)and tumor necrosis factor(TNF-a)in the serum of different groups of mice were detected to screen out the extraction fractions of A.caespitosa with good antitussive and anti-inflammatory activity.[Results]Antitussive experiments on mice showed that the water layer extraction fraction,ethyl acetate extraction fraction and petroleum ether extraction fraction of Miao medicine A.caespitosa could prolong the incubation period of cough in mice and reduce the number of coughs within 2 min to varying degrees,and its different extraction fractions all had different inhibitory effects on mouse ear swelling,granuloma and footpad swelling.Compared with the blank control group,the different extraction fractions could reduce IL-6,TNF-a and other indicators to different degrees.[Conclusions]Ethyl acetate extraction fraction of A.caespitosa has good antitussive and anti-inflammatory effects.展开更多
基金Scientific and Technological Research Project of Traditional Chinese Medicine and Ethnic Medicine of Guizhou Administration of Traditional Chinese Medicine(QZYY2017-116)Scientific Research Project of Guizhou University of Traditional Chinese Medicine(GZYKYN[2017]28).
文摘[Objectives]This study was conducted to investigate the antitussive and anti-inflammatory effects of Miao medicine Aspidistra caespitosa C.Pei.[Methods]A mouse cough model was made by the SO 2 cough induction method,and the antitussive effects of different extraction fractions of A.caespitosa were observed.The inflammation models of acute inflammatory ear swelling in mice caused by xylene,granuloma in mice caused by cotton balls,and footpad swelling in mice caused by carrageenan were made,and the different extraction fractions were used to observe the effects of different extraction fractions on mouse ear swelling,granuloma and footpad swelling.And the levels of interleukin 6(IL-6)and tumor necrosis factor(TNF-a)in the serum of different groups of mice were detected to screen out the extraction fractions of A.caespitosa with good antitussive and anti-inflammatory activity.[Results]Antitussive experiments on mice showed that the water layer extraction fraction,ethyl acetate extraction fraction and petroleum ether extraction fraction of Miao medicine A.caespitosa could prolong the incubation period of cough in mice and reduce the number of coughs within 2 min to varying degrees,and its different extraction fractions all had different inhibitory effects on mouse ear swelling,granuloma and footpad swelling.Compared with the blank control group,the different extraction fractions could reduce IL-6,TNF-a and other indicators to different degrees.[Conclusions]Ethyl acetate extraction fraction of A.caespitosa has good antitussive and anti-inflammatory effects.
