Hizikia fusiforme polysaccharide(HFP)is a natural sulfated polysaccharide derived from brown algae that modulates gut microbiota to confer health benefits.However,the key bacteria targeted by HFP for utilization in th...Hizikia fusiforme polysaccharide(HFP)is a natural sulfated polysaccharide derived from brown algae that modulates gut microbiota to confer health benefits.However,the key bacteria targeted by HFP for utilization in the gut microbiota remain unclear.In the present study,HFP showed high resistance against in vitro digestion in the upper gastrointestinal tract but was fermentable by in vitro gut microbiota(human feces),as evidenced by reduced HFP content and decreased sulfate group content after fermentation.Seven Bacteroides strains were then screened for their HFP-utilizing ability as a single strain.Among them,Bacteroides caccae was highly effective in degrading HFP.Unlike the human fecal microbiota,the sulfate group content of HFP was not significantly reduced by B.caccae.It is worth noting that fermentation by B.caccae reduced the uronic acid content in HFP.A similar phenomenon was not observed when HFP was fermented by human fecal microbiota,indicating that B.caccae was one of the participants in the utilization of HFP in the human gut.In addition,HFP significantly influenced the metabolic profile of B.caccae,facilitating the accumulation of a diverse array of metabolites,with lipids-like molecules and organic acids being predominant.To the best of our knowledge,this is the first report of B.caccae-degrading polysaccharides from Hizikia fusiforme,laying the foundation for further research and enhancing our understanding of HFP’s interactions with human gut commensal bacteria.展开更多
基金supported by Shenzhen Science and Technology Program(ZDSYS20220117155800001)National Natural Science Foundation of China(32302148).
文摘Hizikia fusiforme polysaccharide(HFP)is a natural sulfated polysaccharide derived from brown algae that modulates gut microbiota to confer health benefits.However,the key bacteria targeted by HFP for utilization in the gut microbiota remain unclear.In the present study,HFP showed high resistance against in vitro digestion in the upper gastrointestinal tract but was fermentable by in vitro gut microbiota(human feces),as evidenced by reduced HFP content and decreased sulfate group content after fermentation.Seven Bacteroides strains were then screened for their HFP-utilizing ability as a single strain.Among them,Bacteroides caccae was highly effective in degrading HFP.Unlike the human fecal microbiota,the sulfate group content of HFP was not significantly reduced by B.caccae.It is worth noting that fermentation by B.caccae reduced the uronic acid content in HFP.A similar phenomenon was not observed when HFP was fermented by human fecal microbiota,indicating that B.caccae was one of the participants in the utilization of HFP in the human gut.In addition,HFP significantly influenced the metabolic profile of B.caccae,facilitating the accumulation of a diverse array of metabolites,with lipids-like molecules and organic acids being predominant.To the best of our knowledge,this is the first report of B.caccae-degrading polysaccharides from Hizikia fusiforme,laying the foundation for further research and enhancing our understanding of HFP’s interactions with human gut commensal bacteria.
