为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆...为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆的序列与 Gen Bank中的序列一致。利用线性化的质粒模板反转出 c RNA探针 。展开更多
Objective: To establish a sensitive, nonradioactive in situ hybridization method to detect the expression of gut regulatory peptide genes. Methods: The digoxigenin (Dig) labeled somatostatin (SS) RNA probes were synth...Objective: To establish a sensitive, nonradioactive in situ hybridization method to detect the expression of gut regulatory peptide genes. Methods: The digoxigenin (Dig) labeled somatostatin (SS) RNA probes were synthesized with in vitro transcription system and the in situ hybridization was conducted on the cryostat sections of rat stomach , duodenum and pancreas. Results: Positive reaction sites were purple-blue in color and located in the cytoplasm with a light background and a strong contrast. Both the morphosis and distribution of the SS mRNA posrtive cells corresponded well with those of the previously reported SS immunoreactive cells. Conclusion: The Dig -labeled cRNA probe in situ hybridization method is simple, sensitive and reliable for the study of gut regulatory peptide gene expression.展开更多
The Crna River is a river in the Republic of Macedonia, right tributary to Vardar. Its source is in the mountains of Western Macedonia, west of Krusevo. It flows through the village of Sopotnica, and southwards throug...The Crna River is a river in the Republic of Macedonia, right tributary to Vardar. Its source is in the mountains of Western Macedonia, west of Krusevo. It flows through the village of Sopotnica, and southwards through the plains east of Bitola. The name means ＂black river＂ in Macedonian, which is translation for its former Thracian name. The purpose of this paper is to show the hunting and hunting areas for big and small Game （food）, the structure of the areas of certain hunting, fishing, fishing water objects, fish species, fishponds up to 20 years shown by municipalities and individual farms with ponds in the basin of Black River. It had used statistical data for the hunting grounds by areas, fishing ponds. Fish is very diverse in this river. Hunting in the Black River basin is of great importance for the economy of Republic of Macedonia. It is also a necessary factor for development of other economic activities.展开更多
文摘为了研究褪黑素受体在人脑中的表达状况以及褪黑素在老年性痴呆中的作用 ,本研究克隆了 Mel1 a和 RORβ等两种褪黑素受体的 c DNA并将其转入 p Bluescript ( KS+)质粒载体中。重组质粒通过限制性内切酶鉴定分析并测序。结果表明 ,克隆的序列与 Gen Bank中的序列一致。利用线性化的质粒模板反转出 c RNA探针 。
文摘Objective: To establish a sensitive, nonradioactive in situ hybridization method to detect the expression of gut regulatory peptide genes. Methods: The digoxigenin (Dig) labeled somatostatin (SS) RNA probes were synthesized with in vitro transcription system and the in situ hybridization was conducted on the cryostat sections of rat stomach , duodenum and pancreas. Results: Positive reaction sites were purple-blue in color and located in the cytoplasm with a light background and a strong contrast. Both the morphosis and distribution of the SS mRNA posrtive cells corresponded well with those of the previously reported SS immunoreactive cells. Conclusion: The Dig -labeled cRNA probe in situ hybridization method is simple, sensitive and reliable for the study of gut regulatory peptide gene expression.
文摘The Crna River is a river in the Republic of Macedonia, right tributary to Vardar. Its source is in the mountains of Western Macedonia, west of Krusevo. It flows through the village of Sopotnica, and southwards through the plains east of Bitola. The name means ＂black river＂ in Macedonian, which is translation for its former Thracian name. The purpose of this paper is to show the hunting and hunting areas for big and small Game （food）, the structure of the areas of certain hunting, fishing, fishing water objects, fish species, fishponds up to 20 years shown by municipalities and individual farms with ponds in the basin of Black River. It had used statistical data for the hunting grounds by areas, fishing ponds. Fish is very diverse in this river. Hunting in the Black River basin is of great importance for the economy of Republic of Macedonia. It is also a necessary factor for development of other economic activities.
