目的:观察蒲黄总黄酮(Pollen Typhae total flavones,PTF)对棕榈酸培养下C2C12骨骼肌细胞白细胞介素6(interleukin-6,IL-6)表达的影响,探讨PTF改善骨骼肌胰岛素抵抗(insulin resistance,IR)的可能机制。方法:0.25mmol/L棕榈酸培养建立...目的:观察蒲黄总黄酮(Pollen Typhae total flavones,PTF)对棕榈酸培养下C2C12骨骼肌细胞白细胞介素6(interleukin-6,IL-6)表达的影响,探讨PTF改善骨骼肌胰岛素抵抗(insulin resistance,IR)的可能机制。方法:0.25mmol/L棕榈酸培养建立骨骼肌细胞IR模型。根据处理方法不同,设对照组、模型组、核转录因子κB(nuclear factor-kappaB,NF-κB)抑制剂PDTC组、罗格列酮(rosiglitazone,ROS)组、ROS+PDTC组、PTF组和PTF+PDTC抑制剂组。处理16h后,3H-脱氧葡萄糖摄入法观察细胞对葡萄糖的转运率,实时定量多聚酶联反应检测IL-6mRNA的表达,酶联免疫吸附测定法检测细胞培养上清中IL-6蛋白水平。结果:0.25mmol/L棕榈酸培养16h,C2C12细胞葡萄糖摄取率下降30.43%,IR模型建立;PTF可使IR模型细胞葡萄糖转运率增加32.39%,IL-6mRNA表达及细胞培养上清液中IL-6蛋白水平均显著下降,差异有统计学意义(P<0.05);加入PDTC后,细胞IL-6mRNA表达及细胞培养上清液中IL-6蛋白水平上升(P<0.05)。结论:PTF通过抑制NF-κB通路而抑制C2C12骨骼肌细胞IL-6mRNA表达及蛋白分泌,可能是其减轻骨骼肌炎症状态和改善IR的机制之一。展开更多
Catalytic steam reforming of condensable vapors, i.e. bio-oil, derived from pyrolysis of biomass is an important process for hydrogen production, which is expected to form renewable and clean energy. The generation of...Catalytic steam reforming of condensable vapors, i.e. bio-oil, derived from pyrolysis of biomass is an important process for hydrogen production, which is expected to form renewable and clean energy. The generation of hydrogen from bio-oil was investigated from 250 to 750 ℃ by a MgO mixed C12A7-O^-(C12A7-MgO) catalyst in a fixed-bed micro-reactor. The hydrogen yield on C12A7-MgO was about 44% at 750 ℃. It is found that both the catalytic activity and catalysis life are improved by doping MgO. The XRD results show that the C12A7 structure of the positively charged lattice framework remains in the C12A7-MgO catalyst.展开更多
文摘Catalytic steam reforming of condensable vapors, i.e. bio-oil, derived from pyrolysis of biomass is an important process for hydrogen production, which is expected to form renewable and clean energy. The generation of hydrogen from bio-oil was investigated from 250 to 750 ℃ by a MgO mixed C12A7-O^-(C12A7-MgO) catalyst in a fixed-bed micro-reactor. The hydrogen yield on C12A7-MgO was about 44% at 750 ℃. It is found that both the catalytic activity and catalysis life are improved by doping MgO. The XRD results show that the C12A7 structure of the positively charged lattice framework remains in the C12A7-MgO catalyst.
