A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface supercond...A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface superconducting gaps and topological surface states have been observed.However,the simultaneous presence of bulk and surface superconductivity has remained unresolved.In this study,we directly visualize coexisting bulk and surface superconducting gaps in trigonal PtBi2 using ultra-low-temperature scanning tunneling microscopy/spectroscopy.The bulk gap,Δ,is∼0.053 meV,with a critical temperature(T_(c))of∼0.5K and a critical field below 0.01 T,accompanied by a vortex lattice and vortex bound states and yielding a coherence length of∼200 nm.Remarkably,certain surface regions exhibit a much larger gap(Δ)of∼0.42 meV,persisting up to a T_(c)value of∼3K and surviving magnetic fields of up to 2 T,yet lacking a static vortex lattice.This coexistence of robust surface and bulk superconductivity establishes γ-PtBi_(2)as a unique platform for exploring the interplay between bulk and surface Cooper pairings in topological superconductors.展开更多
The organic compound composition ofwastewater,serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activate...The organic compound composition ofwastewater,serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activated sludge.This study focused on the impact of typical soluble and slowly-biodegradable organic compounds,investigating the pathways through which these substrates affect the occurrence of filamentous bulking in systems operated under both high-and low-oxygen conditions.Results showed that slowly-biodegradable organic compounds lead to a concentrated distribution of microorganisms within flocs,with inward growth of filamentous bacteria.Both Tween-80 and granular starch treated systems exhibited a significant increase in protein content.The glucose system,utilizing soluble substrates,exhibited a markedly higher total polysaccharide content.Microbial communities in the Tween-80 and granular starch treated systems were characterized by a higher abundance of bacteria known to enhance sludge flocculation and settling,such as Competibacter,Xanthomonadaceae and Zoogloea.These findings are of high significance for controlling the operational performance and stability of activated sludge systems,deepening our understanding and providing a novel perspective for the improvement of wastewater treatment processes.展开更多
目的基于网络拓扑学、Bulk转录组学和空间转录组学探究绿茶多酚抗胰腺癌(pancreatic adenocarcinoma,PAAD)的分子机制。方法利用网络拓扑学分析绿茶多酚抗PAAD的潜在作用靶点,基于BEST平台和GSCA数据库评估靶基因的富集通路和肿瘤免疫...目的基于网络拓扑学、Bulk转录组学和空间转录组学探究绿茶多酚抗胰腺癌(pancreatic adenocarcinoma,PAAD)的分子机制。方法利用网络拓扑学分析绿茶多酚抗PAAD的潜在作用靶点,基于BEST平台和GSCA数据库评估靶基因的富集通路和肿瘤免疫微环境,通过SpatialTME数据库探究靶基因的空间作用机制。结果绿茶多酚能够通过多条通路和多靶点干预PAAD的发生发展。靶基因表达水平与受体酪氨酸激酶(receptor tyrosine kinase,RTK)信号通路呈显著正相关关系。此外,靶基因表达与中性粒细胞(neutrophil)、辅助性T细胞2型(T helper 2 cell,Th2)和初始CD4阳性T淋巴细胞(naive CD4-positive T lymphocyte,CD4_naive)浸润丰度呈显著负相关关系(P<0.05),而与黏膜相关恒定T细胞(mucosal-associated invariant T cells,MAIT)、CD4阳性T淋巴细胞(CD4-positive T lymphocyte,CD4_T)和1型调节性T细胞(type 1 regulatory T cell,Tr1)等丰度呈显著正相关关系(P<0.05)。靶基因在免疫治疗抗程序性细胞死亡蛋白1(programmed cell death protein 1,PD-1)和抗PD-1/程序性死亡配体1(PD-1/programmed death-ligand 1,PD-1/PD-L1)队列中能够显著区分有反应者和无反应者。结论本研究揭示了绿茶多酚干预PAAD的组织定位机制,提示靶基因有望作为PAAD的肿瘤标志物,对其临床诊断和治疗具有重要意义。展开更多
The growth of Caenorhabditis elegans involves multiple molting processes,during which old cuticles are shed and new cuticles are rapidly formed.This process requires the regulated bulk secretion of cuticle components....The growth of Caenorhabditis elegans involves multiple molting processes,during which old cuticles are shed and new cuticles are rapidly formed.This process requires the regulated bulk secretion of cuticle components.The transmembrane protein-39(TMEM-39)mutant exhibits distinct dumpy and ruptured phenotypes characterized by notably thin cuticles.TMEM-39 primarily co-localizes with the coat protein II complex(COPII)in large vesicles rather than small COPII vesicles.These TMEM-39-associated large vesicles(TMEM-39-LVs)form robustly during the molting period and co-localize with various extracellular matrix components,including BLI-1 collagen,BLI-3 dual oxidase,and carboxypeptidases.Through immunoprecipitation using TMEM39A-FLAG and proteomics analysis in human sarcoma cells,we identify TMEM39A-associated proteins,including TMEM131.Knockdown of TMEM131 results in reduced TMEM39A-LV formation and collagen secretion in both C.elegans and human sarcoma cells,indicating a cooperative role between TMEM39A and TMEM131 in the secretion of extracellular components through the formation of large COPII vesicles.Given the conservation of TMEM39A and its associated proteins between C.elegans and humans,TMEM39A-LVs may represent a fundamental machinery for rapid and extensive secretion across metazoans.展开更多
To advance materials with superior performance,the construction of gradient structures has emerged as a promising strategy.In this study,a gradient nanocrystalline-amorphous structure was induced in Zr46Cu46Al8 bulk m...To advance materials with superior performance,the construction of gradient structures has emerged as a promising strategy.In this study,a gradient nanocrystalline-amorphous structure was induced in Zr46Cu46Al8 bulk metallic glass(BMG)through ultrasonic vibration(UV)treatment.Applying a 20 kHz ultrasonic cyclic loading in the elastic regime,controllable gradient structures with varying crystallized volume fractions can be achieved in less than 2 s by adjusting the input UV energy.In contrast to tradi-tional methods of inducing structural gradients in BMGs,this novel approach offers distinct advantages:it is exceptionally rapid,requires minimal stress,and allows for easy tuning of the extent of structural gradients through precise adjustment of processing parameters.Nanoindentation tests reveal higher hard-ness near the struck surface,attributed to a greater degree of nanocrystal formation,which gradually di-minishes with depth.As a result of the gradient dispersion of nanocrystals,an increased plasticity was found after UV treatment,characterized by the formation of multiple shear bands.Microstructural in-vestigations suggest that UV-induced nanocrystallization originates from local atomic rearrangements in phase-separated Cu-rich regions with high diffusional mobility.Our study underscores the tunability of structural gradients and corresponding performance improvements in BMGs through ultrasonic energy modulation,offering valuable insights for designing advanced metallic materials with tailored mechanical properties.展开更多
Bulk modulus is a constant that measures the incompressibility of materials, which can be obtained in high pressure experiment by fitting the equations of state(EOS), like third-order Birch–Murnaghan EOS(BM EOS) and ...Bulk modulus is a constant that measures the incompressibility of materials, which can be obtained in high pressure experiment by fitting the equations of state(EOS), like third-order Birch–Murnaghan EOS(BM EOS) and Vinet EOS. Bulk modulus reflects the intermolecular interaction inside molecular crystals, making it useful for researchers to design novel high pressure materials. This review systematically examines bulk moduli of various molecular crystals, including rare-gas solids, di-atom and triplet-atom molecules, saturated organic molecules, and aromatic organic crystals. Comparisons with ionic crystals are presented, along with an analysis of connections between bulk modulus and crystal structures.展开更多
To understand the differences in the composition and sources of PM_(2.5) and PM_(10) caused by coarse particles,integrated PM_(2.5) and PM_(10) samples were synchronously collected in Nanjing,East China,in summer 2020...To understand the differences in the composition and sources of PM_(2.5) and PM_(10) caused by coarse particles,integrated PM_(2.5) and PM_(10) samples were synchronously collected in Nanjing,East China,in summer 2020 and winter 2020/2021.Bulk and molecular speciation and light absorption measurements of aerosol extractswere performed,followed by positivematrix factorization(PMF)based on the PM_(2.5) and PM_(10) data sets,respectively.The difference in average concentrations of total bulk species between PM_(2.5) and PM_(10) was mainly caused by the distribution of considerable NO_(3)^(–),SO42–,Ca^(2+),and organic carbon(OC)in coarse particles.Coarse PMinfluenced by abrasion products from tirewear and leaves contributed about half of the low-volatility n-alkanes in summer.The contribution of coarse PM to biomass burning tracers and water-soluble OC increased in winter when biomass combustion was excessively active.More than 70%of sugar polyols were attributable to coarse PM in summer,and biomass burning could be an important source in winter.The light-absorbing organic chromophores were almost entirely associated with PM_(2.5),but water-soluble organic carbon(WSOC)exhibited stronger light absorption in PM_(10) extracts than in PM_(2.5) extracts possibly due to the influence of coarse PMon pH.PMF analysis indicated that biomass burning,aqueous-phase reactions,and processed dust were the main contributors of organic matter and its light absorption in winter.Biogenic primary and secondary sources made discernable contributions only in summer.The differences between PM_(2.5) and PM_(10) were likely attributed to mixing of crustal dust,combustion particles,and surface reactions.展开更多
Water depth significantly affects ship resistance,which,in turn,influences fuel consumption.Furthermore,the urgent need to reduce carbon emissions for environmental sustainability highlights the importance of applying...Water depth significantly affects ship resistance,which,in turn,influences fuel consumption.Furthermore,the urgent need to reduce carbon emissions for environmental sustainability highlights the importance of applying drag reduction methods to shallow-water vehicles.To effectively employ these methods,the initial step entails an in-depth investigation of how shallow water impacts the resistance and flow dynamics of a mini-bulk carrier.This study extensively analyzes the hydrodynamic characteristics of mini-bulk carriers,focusing on the impact of shallow water on resistance and flow dynamics utilizing a combination of experimental tests and numerical analyses.This study emphasizes the interaction between the hull and the shallow seabed.This study also highlights increased frictional drag and significant residual resistance by analyzing the total resistance at various speeds in shallow waters.The results of five key factors influencing resistance in shallow waters,namely,boundary layer thickness,shear stress,velocity and pressure,turbulence,and waves,are discussed.A decrease in water depth accelerates the flow under the hull,increasing shear stress and resistance.The accelerated flow reduces the gap between the hull and the shallow seabed,elevating water pressure and increasing sinkage and resistance.Heightened turbulence in shallow water intensifies Reynolds stress,augmenting friction and viscous resistance.展开更多
Large-grain REBa_(2)Cu_(3)O_(7-δ)(REBCO,RE=rare earth)bulk superconductors offer promising magnetic field trapping capabilities due to their high critical current density,making them ideal for many important applicat...Large-grain REBa_(2)Cu_(3)O_(7-δ)(REBCO,RE=rare earth)bulk superconductors offer promising magnetic field trapping capabilities due to their high critical current density,making them ideal for many important applications such as trapped field magnets.However,for such large-grain superconductor bulks,there are lots of voids and cracks forming during the process of melting preparation,and some of them can be up to hundreds of microns or even millimeters in size.Consequently,these larger size voids/cracks pose a great threat to the strength of the bulks due to the inherent brittleness of superconductor REBCO materials.In order to ensure the operational safety of related superconducting devices with bulk superconductors,it is firstly important to accurately detect these voids/cracks in them.In this paper,we proposed a method for quantitatively evaluating multiple voids/cracks in bulk superconductors through the magnetic field and displacement response signals at superconductor bulk surface.The proposed method utilizes a damage index constructed from the magnetic field signals and displacement responses to identify the number and preliminary location of multiple defects.By dividing the detection area into subdomains and combining the magnetic field signals with displacement responses within each subdomain,a particle swarm algorithm was employed to evaluate the location and size parameters of the defects.In contrast to other evaluation methods using only magnetic field or displacement response signals,the combined evaluation method using both signals can identify the number of cracks effectively.Numerical studies demonstrate that the morphology of voids and cracks reconstructed using the proposed algorithm ideally matches real defects and is applicable to cases where voids and cracks coexist.This study provides a theoretical basis for the quantitative detection of voids/cracks in bulk superconductors.展开更多
Ti-based bulk metallic glasses(BMGs)have attracted increasing attention due to their high specific strength.However,a fundamental conflict exists between the specific strength and glass-forming ability(GFA)of Ti-based...Ti-based bulk metallic glasses(BMGs)have attracted increasing attention due to their high specific strength.However,a fundamental conflict exists between the specific strength and glass-forming ability(GFA)of Ti-based BMGs,restricting their commercial applications significantly.In this study,this challenge was addressed by introducing a two-step alloying strategy to mitigate the remarkable density increment effect associated with heavy alloying elements required for enhancing the GFA.Consequently,through two-step alloying with Al and Fe in sequence,simultaneous enhancements in specific strength and GFA were achieved based on a Ti-Zr-Be ternary metallic glass,resulting in the development of a series of centimeter-sized metallic glasses exhibiting ultrahigh-specific strength.Notably,the newly developed(Ti_(45)Zr_(20)Be_(31)A_(l4))_(94)Fe_(6)alloy established a new record for the specific strength of Ti-based BMGs.Along with a critical diameter(D_(c))of 10 mm,it offers the optimal scheme for balancing the specific strength and GFA of Ti-based BMGs.The present results further brighten the application prospects of Ti-based BMGs as lightweight materials.展开更多
The improvement of soybean seed carotenoid contents is very important due to the beneficial role of carotenoids in human health and nutrition. However, the genetic architecture underlying soybean carotenoid biosynthes...The improvement of soybean seed carotenoid contents is very important due to the beneficial role of carotenoids in human health and nutrition. However, the genetic architecture underlying soybean carotenoid biosynthesis remains largely unknown. In the present study, we employed next generation sequencing-based bulked-segregant analysis to identify new genomic regions governing seed carotenoids in 1,551 natural soybean accessions. The genomic DNA samples of individual plants with extreme phenotypes were pooled to form two bulks with high(50 accessions) and low(50 accessions) carotenoid contents for Illumina sequencing. A total of 125.09 Gb of clean bases and 89.82% of Q30 were obtained, and the average alignment efficiency was 99.45% with an average coverage depth of 62.20× and 99.75% genome coverage. Based on the G prime statistic algorithm(G') method analysis, 16 candidate genomic loci with a total length 20.41 Mb were found to be related to the trait. Of these loci, the most significant regions displaying the highest elevated G' values were found in chromosome 06 at a position of 18.53–22.67 Mb, and chromosome 19 at genomic region intervals of 8.36–10.94, 12.06–13.79 and 18.45–20.26 Mb. These regions were then used to identify the key candidate genes. In these regions, 250 predicted genes were found and analyzed to obtain 90 significantly enriched(P<0.05) Gene Ontology(GO) terms. Based on ANNOVAR analysis, 50 genes with non-synonymous and stopgained mutations were preferentially selected as potential candidate genes. Of those 50 genes, following their gene annotation functions and high significant haplotype variations in various environments,five genes were identified as the most promising candidate genes regulating soybean seed carotenoid accumulation, and they should be investigated in further functional validation studies. Collectively, understanding the genetic basis of carotenoid pigments and identifying genes underpinning carotenoid accumulation via a bulked-segregant analysis-based sequencing(BSA-seq) approach provide new insights for exploring future molecular breeding efforts to produce soybean cultivars with high carotenoid content.展开更多
Avoiding crystallization while maintaining the original microstructure and mechanical properties of the material are long-term goals of laser welding of Zr-based bulk metallic glass(BMG).In this paper,the effect of pu...Avoiding crystallization while maintaining the original microstructure and mechanical properties of the material are long-term goals of laser welding of Zr-based bulk metallic glass(BMG).In this paper,the effect of pulsed laser welding parameters on the microstructure,crystallization degree,and mechanical properties of Zr57Nb5Cu15.4Ni12.6Al10 BMG is investigated.Non-crystallized welding forming of a zirconium-based amorphous alloy is achieved by optimizing the process parameters of pulsed laser welding.The crystallization degree of Zr-based BMG is mainly determined by the welding speed and power.The welding depth and crystallization area fraction increase with an increase in the effective peak power density.The optimized welding process can effectively reduce the heat accumulation of the weld,thus avoiding crystallization.The flexural strength of the weld can be maintained at 96.5%of the matrix.展开更多
The copper-cerium catalysts demonstrate high efficiency in CO_(2)reduction reactions(CO_(2)RR).However,the mechanism governing the formation of C_(2)H_(4)and CH_(4)by regulating Cu bulk phase structure at the copper-c...The copper-cerium catalysts demonstrate high efficiency in CO_(2)reduction reactions(CO_(2)RR).However,the mechanism governing the formation of C_(2)H_(4)and CH_(4)by regulating Cu bulk phase structure at the copper-cerium interface remains unclear due to the instability and dynamic evaluations of copper species.Herein,we synthesized CeO_(2)-CuO containing solely Cu^(2+)species and CeO_(2)-Cu featuring predominantly metallic Cu species at the interface,which exhibit stable structures under various potentials,offering ideal models for in-depth mechanistic studies.The C_(2)H_(4)is the main product over the CeO_(2)-CuO catalyst,exhibiting a Faradaic efficiency(FE)of 42.3%±1.4%,while CH_(4)is the primary product over the CeO_(2)-Cu catalyst,with a FE of 32.4%±1.3%.These results demonstrate that regulating bulk phase Cu structure at the copper-cerium interface influences the selectivity of hydrocarbon products.The operando ATR-SEIRAS finds that CeO_(2)-CuO surfaces with single linear*CO adsorption are advantageous for synthesizing*COCO,whereas bridge-bonded*CO adsorption promoted*CHO formation.Furthermore,DFT simulations demonstrate that the energy barrier of CO-CO coupling(C_(2)H_(4)pathway)at the CeO_(2)-CuO interface decreases as compared to the CeO_(2)-Cu catalyst,thus indicating a facilitated conversion of the CO_(2)to C_(2)H_(4).This research deepens the mechanistic understanding of the copper-cerium system during CO_(2)RR and effectively formulates a strategy for developing high-selectivity catalysts.展开更多
Accurate detection of dimethyl methylphosphonate(DMMP),a simulant for chemical warfare agents,is vital for both public safety and military defense.However,conventional detection methods suffer from low selectivity,owi...Accurate detection of dimethyl methylphosphonate(DMMP),a simulant for chemical warfare agents,is vital for both public safety and military defense.However,conventional detection methods suffer from low selectivity,owing to interference from structurally similar compounds.In this study,we present a highly sensitive and selective gas sensor utilizing a solid-mounted film bulk acoustic resonator based on carbon nanotubes functionalized with hexafluoroisopropanol(HFiP)to enhance DMMP detection.This approach leverages the strong hydrogen bonding between HFiP and DMMP molecules to significantly improve the sensor’s adsorption capacity and selectivity.To further refine selectivity and at the same time solve the cross-sensitivity problem of sensitive membranes,we introduce a virtual sensor array design,generated by modulating the input power to the resonator,which enables the sensor to operate in multiple response modes across varying vibrational amplitudes.These multimodal responses are subjected to linear discriminant analysis,allowing precise differentiation of DMMP from other volatile organic compounds such as tributyl phosphate and dimethyl phthalate.Our results demonstrate superior performance in terms of both sensitivity and selectivity,offering a robust solution for detecting low-concentration DMMP in complex environments.展开更多
1.Introduction The synthesis of bulk nanostructured multiphase(NM)mate-rials with extreme properties such as high hardness and strength is one of the most interesting research topics in materials science and engineeri...1.Introduction The synthesis of bulk nanostructured multiphase(NM)mate-rials with extreme properties such as high hardness and strength is one of the most interesting research topics in materials science and engineering[1].At present,NM alloys can be produced by several synthesis methods,including sintering of nanocomposites[2,3],physical or chemical vapour deposition(PVD or CVD)[4],crystallization of metallic glasses[5],and severe plastic deforma-tion(SPD)[6-8].However,industry applications of bulk NM alloys produced by these methods are significantly restricted by their ge-ometrical and size limitations.Thus,the fabrication of large-scale NM alloys remains challenging.展开更多
An interesting phenomenon of cooling-rate induced brittleness in Zr52.5Cu17.9Ni14.6Al10Ti5 bulk metallic glass （BMG） was reported. It was found that the as-cast BMG specimens exhibited a brittle-ductile transition w...An interesting phenomenon of cooling-rate induced brittleness in Zr52.5Cu17.9Ni14.6Al10Ti5 bulk metallic glass （BMG） was reported. It was found that the as-cast BMG specimens exhibited a brittle-ductile transition when the larger specimens were machined into smaller specimens through removing the cast-softening surface layer by layer. After compression tests, the as-machined small specimens, owing to the absence of the cast-softening surface, displayed highly dense and intersecting shear bands, and extensive plastic deformation. This is in contrast to the catastrophic failure and low deformability in the as-cast large specimens. More free volume was detected in the smaller as-fractured specimens, by differential scanning calorimetry, which may be attributed to the occurrence of strain softening and increased plasticity. Compared with the relatively smooth fracture surface in the smaller specimens, the larger specimens showed more diverse features on the fracture surface due to their graded structures.展开更多
Gassmann＇s equations are commonly used for predicting seismic wave velocity in rock physics research.However the input matrix mineral bulk modulus parameters are not accurate,which greatly influences the prediction r...Gassmann＇s equations are commonly used for predicting seismic wave velocity in rock physics research.However the input matrix mineral bulk modulus parameters are not accurate,which greatly influences the prediction reliability.In this paper,combining the Russell fluid factor with the Gassman-Biot-Geertsma equation and introducing the dry-rock Poisson＇s ratio,we propose an effective matrix mineral bulk modulus extraction method.This method can adaptively invert the equivalent matrix mineral bulk modulus to apply the Gassmann equation to fluid substitution of complex carbonate reservoirs and increase the fluid prediction reliability.The verification of the actual material fluid substitution also shows that this method is reliable,efficient,and adaptable.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.62488201)the National Key Research and Development Projects of China(Grant Nos.2022YFA1204100 and 2023YFA1607400)+1 种基金the CAS Project for Young Scientists in Basic Research(Grant No.YSBR-003)the Innovation Program of Quantum Science and Technology(Grant No.2021ZD0302700)。
文摘A bulk superconductor hosting intrinsic surface superconductivity provides a unique platform for exploring Majorana bound states.Trigonal γ-PtBi_(2),a superconductor,is a promising candidate,as both surface superconducting gaps and topological surface states have been observed.However,the simultaneous presence of bulk and surface superconductivity has remained unresolved.In this study,we directly visualize coexisting bulk and surface superconducting gaps in trigonal PtBi2 using ultra-low-temperature scanning tunneling microscopy/spectroscopy.The bulk gap,Δ,is∼0.053 meV,with a critical temperature(T_(c))of∼0.5K and a critical field below 0.01 T,accompanied by a vortex lattice and vortex bound states and yielding a coherence length of∼200 nm.Remarkably,certain surface regions exhibit a much larger gap(Δ)of∼0.42 meV,persisting up to a T_(c)value of∼3K and surviving magnetic fields of up to 2 T,yet lacking a static vortex lattice.This coexistence of robust surface and bulk superconductivity establishes γ-PtBi_(2)as a unique platform for exploring the interplay between bulk and surface Cooper pairings in topological superconductors.
基金supported by the Opening Project of National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,and the National Natural Science Foundation of China(No.52270017).
文摘The organic compound composition ofwastewater,serves as a crucial indicator for the operational performance of activated sludge processes and has a major influence on the development of filamentous bulking in activated sludge.This study focused on the impact of typical soluble and slowly-biodegradable organic compounds,investigating the pathways through which these substrates affect the occurrence of filamentous bulking in systems operated under both high-and low-oxygen conditions.Results showed that slowly-biodegradable organic compounds lead to a concentrated distribution of microorganisms within flocs,with inward growth of filamentous bacteria.Both Tween-80 and granular starch treated systems exhibited a significant increase in protein content.The glucose system,utilizing soluble substrates,exhibited a markedly higher total polysaccharide content.Microbial communities in the Tween-80 and granular starch treated systems were characterized by a higher abundance of bacteria known to enhance sludge flocculation and settling,such as Competibacter,Xanthomonadaceae and Zoogloea.These findings are of high significance for controlling the operational performance and stability of activated sludge systems,deepening our understanding and providing a novel perspective for the improvement of wastewater treatment processes.
文摘目的基于网络拓扑学、Bulk转录组学和空间转录组学探究绿茶多酚抗胰腺癌(pancreatic adenocarcinoma,PAAD)的分子机制。方法利用网络拓扑学分析绿茶多酚抗PAAD的潜在作用靶点,基于BEST平台和GSCA数据库评估靶基因的富集通路和肿瘤免疫微环境,通过SpatialTME数据库探究靶基因的空间作用机制。结果绿茶多酚能够通过多条通路和多靶点干预PAAD的发生发展。靶基因表达水平与受体酪氨酸激酶(receptor tyrosine kinase,RTK)信号通路呈显著正相关关系。此外,靶基因表达与中性粒细胞(neutrophil)、辅助性T细胞2型(T helper 2 cell,Th2)和初始CD4阳性T淋巴细胞(naive CD4-positive T lymphocyte,CD4_naive)浸润丰度呈显著负相关关系(P<0.05),而与黏膜相关恒定T细胞(mucosal-associated invariant T cells,MAIT)、CD4阳性T淋巴细胞(CD4-positive T lymphocyte,CD4_T)和1型调节性T细胞(type 1 regulatory T cell,Tr1)等丰度呈显著正相关关系(P<0.05)。靶基因在免疫治疗抗程序性细胞死亡蛋白1(programmed cell death protein 1,PD-1)和抗PD-1/程序性死亡配体1(PD-1/programmed death-ligand 1,PD-1/PD-L1)队列中能够显著区分有反应者和无反应者。结论本研究揭示了绿茶多酚干预PAAD的组织定位机制,提示靶基因有望作为PAAD的肿瘤标志物,对其临床诊断和治疗具有重要意义。
基金supported by the National Institutes of Health-Office of Research Infrastructure Programs(P40 OD010440)supported in part by grants from the National Cancer Center of Korea(NCC-2110160,NCC-2110263,and NCC-2310750)supported by the Basic Science Research Program of the National Research Foundation of Korea,funded by the Ministry of Science,ICT,and Future Planning(NRF-2015R1C1A1A01053611).
文摘The growth of Caenorhabditis elegans involves multiple molting processes,during which old cuticles are shed and new cuticles are rapidly formed.This process requires the regulated bulk secretion of cuticle components.The transmembrane protein-39(TMEM-39)mutant exhibits distinct dumpy and ruptured phenotypes characterized by notably thin cuticles.TMEM-39 primarily co-localizes with the coat protein II complex(COPII)in large vesicles rather than small COPII vesicles.These TMEM-39-associated large vesicles(TMEM-39-LVs)form robustly during the molting period and co-localize with various extracellular matrix components,including BLI-1 collagen,BLI-3 dual oxidase,and carboxypeptidases.Through immunoprecipitation using TMEM39A-FLAG and proteomics analysis in human sarcoma cells,we identify TMEM39A-associated proteins,including TMEM131.Knockdown of TMEM131 results in reduced TMEM39A-LV formation and collagen secretion in both C.elegans and human sarcoma cells,indicating a cooperative role between TMEM39A and TMEM131 in the secretion of extracellular components through the formation of large COPII vesicles.Given the conservation of TMEM39A and its associated proteins between C.elegans and humans,TMEM39A-LVs may represent a fundamental machinery for rapid and extensive secretion across metazoans.
基金supported by the Key Basic and Applied Research Program of Guangdong Province,China(Grant No.2019B030302010)the NSF of China(Grant Nos.52122105,52271150,52201185,52201186,52371160)+1 种基金the Science and Technology Innovation Commission Shenzhen(Grants Nos.RCJC20221008092730037,20220804091920001)the Research Team Cultivation Program of Shenzhen University,Grant No.2023QNT001.
文摘To advance materials with superior performance,the construction of gradient structures has emerged as a promising strategy.In this study,a gradient nanocrystalline-amorphous structure was induced in Zr46Cu46Al8 bulk metallic glass(BMG)through ultrasonic vibration(UV)treatment.Applying a 20 kHz ultrasonic cyclic loading in the elastic regime,controllable gradient structures with varying crystallized volume fractions can be achieved in less than 2 s by adjusting the input UV energy.In contrast to tradi-tional methods of inducing structural gradients in BMGs,this novel approach offers distinct advantages:it is exceptionally rapid,requires minimal stress,and allows for easy tuning of the extent of structural gradients through precise adjustment of processing parameters.Nanoindentation tests reveal higher hard-ness near the struck surface,attributed to a greater degree of nanocrystal formation,which gradually di-minishes with depth.As a result of the gradient dispersion of nanocrystals,an increased plasticity was found after UV treatment,characterized by the formation of multiple shear bands.Microstructural in-vestigations suggest that UV-induced nanocrystallization originates from local atomic rearrangements in phase-separated Cu-rich regions with high diffusional mobility.Our study underscores the tunability of structural gradients and corresponding performance improvements in BMGs through ultrasonic energy modulation,offering valuable insights for designing advanced metallic materials with tailored mechanical properties.
基金Project supported by the National Key Research and Development Program of China (Grant Nos. 2019YFA0708502 and 2023YFA1406200)the National Natural Science Foundation of China (Grant No. 22022101)。
文摘Bulk modulus is a constant that measures the incompressibility of materials, which can be obtained in high pressure experiment by fitting the equations of state(EOS), like third-order Birch–Murnaghan EOS(BM EOS) and Vinet EOS. Bulk modulus reflects the intermolecular interaction inside molecular crystals, making it useful for researchers to design novel high pressure materials. This review systematically examines bulk moduli of various molecular crystals, including rare-gas solids, di-atom and triplet-atom molecules, saturated organic molecules, and aromatic organic crystals. Comparisons with ionic crystals are presented, along with an analysis of connections between bulk modulus and crystal structures.
基金supported by the National Natural Science Foundation of China(Nos.42007325 and 42177211).
文摘To understand the differences in the composition and sources of PM_(2.5) and PM_(10) caused by coarse particles,integrated PM_(2.5) and PM_(10) samples were synchronously collected in Nanjing,East China,in summer 2020 and winter 2020/2021.Bulk and molecular speciation and light absorption measurements of aerosol extractswere performed,followed by positivematrix factorization(PMF)based on the PM_(2.5) and PM_(10) data sets,respectively.The difference in average concentrations of total bulk species between PM_(2.5) and PM_(10) was mainly caused by the distribution of considerable NO_(3)^(–),SO42–,Ca^(2+),and organic carbon(OC)in coarse particles.Coarse PMinfluenced by abrasion products from tirewear and leaves contributed about half of the low-volatility n-alkanes in summer.The contribution of coarse PM to biomass burning tracers and water-soluble OC increased in winter when biomass combustion was excessively active.More than 70%of sugar polyols were attributable to coarse PM in summer,and biomass burning could be an important source in winter.The light-absorbing organic chromophores were almost entirely associated with PM_(2.5),but water-soluble organic carbon(WSOC)exhibited stronger light absorption in PM_(10) extracts than in PM_(2.5) extracts possibly due to the influence of coarse PMon pH.PMF analysis indicated that biomass burning,aqueous-phase reactions,and processed dust were the main contributors of organic matter and its light absorption in winter.Biogenic primary and secondary sources made discernable contributions only in summer.The differences between PM_(2.5) and PM_(10) were likely attributed to mixing of crustal dust,combustion particles,and surface reactions.
基金Supported by the Ministry of Shipping,India(Grant No.OEC/18-19/157/MOSH/RVIJ).
文摘Water depth significantly affects ship resistance,which,in turn,influences fuel consumption.Furthermore,the urgent need to reduce carbon emissions for environmental sustainability highlights the importance of applying drag reduction methods to shallow-water vehicles.To effectively employ these methods,the initial step entails an in-depth investigation of how shallow water impacts the resistance and flow dynamics of a mini-bulk carrier.This study extensively analyzes the hydrodynamic characteristics of mini-bulk carriers,focusing on the impact of shallow water on resistance and flow dynamics utilizing a combination of experimental tests and numerical analyses.This study emphasizes the interaction between the hull and the shallow seabed.This study also highlights increased frictional drag and significant residual resistance by analyzing the total resistance at various speeds in shallow waters.The results of five key factors influencing resistance in shallow waters,namely,boundary layer thickness,shear stress,velocity and pressure,turbulence,and waves,are discussed.A decrease in water depth accelerates the flow under the hull,increasing shear stress and resistance.The accelerated flow reduces the gap between the hull and the shallow seabed,elevating water pressure and increasing sinkage and resistance.Heightened turbulence in shallow water intensifies Reynolds stress,augmenting friction and viscous resistance.
基金supported by the National Natural Science Foundation of China(Grant Nos.12232005 and 12072101).
文摘Large-grain REBa_(2)Cu_(3)O_(7-δ)(REBCO,RE=rare earth)bulk superconductors offer promising magnetic field trapping capabilities due to their high critical current density,making them ideal for many important applications such as trapped field magnets.However,for such large-grain superconductor bulks,there are lots of voids and cracks forming during the process of melting preparation,and some of them can be up to hundreds of microns or even millimeters in size.Consequently,these larger size voids/cracks pose a great threat to the strength of the bulks due to the inherent brittleness of superconductor REBCO materials.In order to ensure the operational safety of related superconducting devices with bulk superconductors,it is firstly important to accurately detect these voids/cracks in them.In this paper,we proposed a method for quantitatively evaluating multiple voids/cracks in bulk superconductors through the magnetic field and displacement response signals at superconductor bulk surface.The proposed method utilizes a damage index constructed from the magnetic field signals and displacement responses to identify the number and preliminary location of multiple defects.By dividing the detection area into subdomains and combining the magnetic field signals with displacement responses within each subdomain,a particle swarm algorithm was employed to evaluate the location and size parameters of the defects.In contrast to other evaluation methods using only magnetic field or displacement response signals,the combined evaluation method using both signals can identify the number of cracks effectively.Numerical studies demonstrate that the morphology of voids and cracks reconstructed using the proposed algorithm ideally matches real defects and is applicable to cases where voids and cracks coexist.This study provides a theoretical basis for the quantitative detection of voids/cracks in bulk superconductors.
基金supported by the National Natural Science Foundation of China(Nos.52271148 and 51871129).
文摘Ti-based bulk metallic glasses(BMGs)have attracted increasing attention due to their high specific strength.However,a fundamental conflict exists between the specific strength and glass-forming ability(GFA)of Ti-based BMGs,restricting their commercial applications significantly.In this study,this challenge was addressed by introducing a two-step alloying strategy to mitigate the remarkable density increment effect associated with heavy alloying elements required for enhancing the GFA.Consequently,through two-step alloying with Al and Fe in sequence,simultaneous enhancements in specific strength and GFA were achieved based on a Ti-Zr-Be ternary metallic glass,resulting in the development of a series of centimeter-sized metallic glasses exhibiting ultrahigh-specific strength.Notably,the newly developed(Ti_(45)Zr_(20)Be_(31)A_(l4))_(94)Fe_(6)alloy established a new record for the specific strength of Ti-based BMGs.Along with a critical diameter(D_(c))of 10 mm,it offers the optimal scheme for balancing the specific strength and GFA of Ti-based BMGs.The present results further brighten the application prospects of Ti-based BMGs as lightweight materials.
基金financially supported by the National Natural Science Foundation of China (32161143033, 32272178, and 32001574)National Key Research and Development Program of China (2021YFD1201605)the Agricultural Science and Technology Innovation Project of CAAS。
文摘The improvement of soybean seed carotenoid contents is very important due to the beneficial role of carotenoids in human health and nutrition. However, the genetic architecture underlying soybean carotenoid biosynthesis remains largely unknown. In the present study, we employed next generation sequencing-based bulked-segregant analysis to identify new genomic regions governing seed carotenoids in 1,551 natural soybean accessions. The genomic DNA samples of individual plants with extreme phenotypes were pooled to form two bulks with high(50 accessions) and low(50 accessions) carotenoid contents for Illumina sequencing. A total of 125.09 Gb of clean bases and 89.82% of Q30 were obtained, and the average alignment efficiency was 99.45% with an average coverage depth of 62.20× and 99.75% genome coverage. Based on the G prime statistic algorithm(G') method analysis, 16 candidate genomic loci with a total length 20.41 Mb were found to be related to the trait. Of these loci, the most significant regions displaying the highest elevated G' values were found in chromosome 06 at a position of 18.53–22.67 Mb, and chromosome 19 at genomic region intervals of 8.36–10.94, 12.06–13.79 and 18.45–20.26 Mb. These regions were then used to identify the key candidate genes. In these regions, 250 predicted genes were found and analyzed to obtain 90 significantly enriched(P<0.05) Gene Ontology(GO) terms. Based on ANNOVAR analysis, 50 genes with non-synonymous and stopgained mutations were preferentially selected as potential candidate genes. Of those 50 genes, following their gene annotation functions and high significant haplotype variations in various environments,five genes were identified as the most promising candidate genes regulating soybean seed carotenoid accumulation, and they should be investigated in further functional validation studies. Collectively, understanding the genetic basis of carotenoid pigments and identifying genes underpinning carotenoid accumulation via a bulked-segregant analysis-based sequencing(BSA-seq) approach provide new insights for exploring future molecular breeding efforts to produce soybean cultivars with high carotenoid content.
基金Supported by Guangdong Major Project of Basic and Applied Research,China(Grant No.2019B030302010)National Natural Science Foundation of China (Grant Nos.51735003,52205456)
文摘Avoiding crystallization while maintaining the original microstructure and mechanical properties of the material are long-term goals of laser welding of Zr-based bulk metallic glass(BMG).In this paper,the effect of pulsed laser welding parameters on the microstructure,crystallization degree,and mechanical properties of Zr57Nb5Cu15.4Ni12.6Al10 BMG is investigated.Non-crystallized welding forming of a zirconium-based amorphous alloy is achieved by optimizing the process parameters of pulsed laser welding.The crystallization degree of Zr-based BMG is mainly determined by the welding speed and power.The welding depth and crystallization area fraction increase with an increase in the effective peak power density.The optimized welding process can effectively reduce the heat accumulation of the weld,thus avoiding crystallization.The flexural strength of the weld can be maintained at 96.5%of the matrix.
基金supported financially by the National Natural Science Foundation of China (22302222, 22072172)the Postdoctoral Science Foundation (2024T170965, 2023M743641)+5 种基金the Youth Innovation Promotion Association CAS (Y2021056)Joint Fund of the Yulin University and the Dalian National Laboratory for Clean Energy (YLU-DNL Fund 2022007)the Major Science and Technology Projects of Shanxi Province (202005D121002)the Special Fund for Science and Technology Innovation Teams of Shanxi Province (202304051001007)the Science and Technology Department of Shanxi Province (202303021222409)the Shanxi Provincial Department of Human and Social Resources Security’s Doctor Introduction Program (2024SHB001)
文摘The copper-cerium catalysts demonstrate high efficiency in CO_(2)reduction reactions(CO_(2)RR).However,the mechanism governing the formation of C_(2)H_(4)and CH_(4)by regulating Cu bulk phase structure at the copper-cerium interface remains unclear due to the instability and dynamic evaluations of copper species.Herein,we synthesized CeO_(2)-CuO containing solely Cu^(2+)species and CeO_(2)-Cu featuring predominantly metallic Cu species at the interface,which exhibit stable structures under various potentials,offering ideal models for in-depth mechanistic studies.The C_(2)H_(4)is the main product over the CeO_(2)-CuO catalyst,exhibiting a Faradaic efficiency(FE)of 42.3%±1.4%,while CH_(4)is the primary product over the CeO_(2)-Cu catalyst,with a FE of 32.4%±1.3%.These results demonstrate that regulating bulk phase Cu structure at the copper-cerium interface influences the selectivity of hydrocarbon products.The operando ATR-SEIRAS finds that CeO_(2)-CuO surfaces with single linear*CO adsorption are advantageous for synthesizing*COCO,whereas bridge-bonded*CO adsorption promoted*CHO formation.Furthermore,DFT simulations demonstrate that the energy barrier of CO-CO coupling(C_(2)H_(4)pathway)at the CeO_(2)-CuO interface decreases as compared to the CeO_(2)-Cu catalyst,thus indicating a facilitated conversion of the CO_(2)to C_(2)H_(4).This research deepens the mechanistic understanding of the copper-cerium system during CO_(2)RR and effectively formulates a strategy for developing high-selectivity catalysts.
基金supported by the State Key Laboratory of Pathogens and Biosecurity(Grant No.SKLPBS2240).
文摘Accurate detection of dimethyl methylphosphonate(DMMP),a simulant for chemical warfare agents,is vital for both public safety and military defense.However,conventional detection methods suffer from low selectivity,owing to interference from structurally similar compounds.In this study,we present a highly sensitive and selective gas sensor utilizing a solid-mounted film bulk acoustic resonator based on carbon nanotubes functionalized with hexafluoroisopropanol(HFiP)to enhance DMMP detection.This approach leverages the strong hydrogen bonding between HFiP and DMMP molecules to significantly improve the sensor’s adsorption capacity and selectivity.To further refine selectivity and at the same time solve the cross-sensitivity problem of sensitive membranes,we introduce a virtual sensor array design,generated by modulating the input power to the resonator,which enables the sensor to operate in multiple response modes across varying vibrational amplitudes.These multimodal responses are subjected to linear discriminant analysis,allowing precise differentiation of DMMP from other volatile organic compounds such as tributyl phosphate and dimethyl phthalate.Our results demonstrate superior performance in terms of both sensitivity and selectivity,offering a robust solution for detecting low-concentration DMMP in complex environments.
基金funding from the Australian Research Council(ARC Discovery Project,Nos.DP200101408 and DP230100183).
文摘1.Introduction The synthesis of bulk nanostructured multiphase(NM)mate-rials with extreme properties such as high hardness and strength is one of the most interesting research topics in materials science and engineering[1].At present,NM alloys can be produced by several synthesis methods,including sintering of nanocomposites[2,3],physical or chemical vapour deposition(PVD or CVD)[4],crystallization of metallic glasses[5],and severe plastic deforma-tion(SPD)[6-8].However,industry applications of bulk NM alloys produced by these methods are significantly restricted by their ge-ometrical and size limitations.Thus,the fabrication of large-scale NM alloys remains challenging.
基金Project(2012M511401)supported by China Postdoctoral Science FoundationProject(12JJ5018)supported by Hunan Provincial Natural Science Foundation of China+1 种基金Project(2012RS4006)supported by Hunan Provincial Science and Technology Plan of ChinaProject(CSUZC2012028)supported by the Open-End Fund for the Valuable and Precision Instruments of Central South University,China
文摘An interesting phenomenon of cooling-rate induced brittleness in Zr52.5Cu17.9Ni14.6Al10Ti5 bulk metallic glass （BMG） was reported. It was found that the as-cast BMG specimens exhibited a brittle-ductile transition when the larger specimens were machined into smaller specimens through removing the cast-softening surface layer by layer. After compression tests, the as-machined small specimens, owing to the absence of the cast-softening surface, displayed highly dense and intersecting shear bands, and extensive plastic deformation. This is in contrast to the catastrophic failure and low deformability in the as-cast large specimens. More free volume was detected in the smaller as-fractured specimens, by differential scanning calorimetry, which may be attributed to the occurrence of strain softening and increased plasticity. Compared with the relatively smooth fracture surface in the smaller specimens, the larger specimens showed more diverse features on the fracture surface due to their graded structures.
基金sponsored by National Natural Science Foundation of China(Grant No.40904035)
文摘Gassmann＇s equations are commonly used for predicting seismic wave velocity in rock physics research.However the input matrix mineral bulk modulus parameters are not accurate,which greatly influences the prediction reliability.In this paper,combining the Russell fluid factor with the Gassman-Biot-Geertsma equation and introducing the dry-rock Poisson＇s ratio,we propose an effective matrix mineral bulk modulus extraction method.This method can adaptively invert the equivalent matrix mineral bulk modulus to apply the Gassmann equation to fluid substitution of complex carbonate reservoirs and increase the fluid prediction reliability.The verification of the actual material fluid substitution also shows that this method is reliable,efficient,and adaptable.
