Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the e...Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concen- trations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8.展开更多
Akram MUHAMMAD, Aftab FAHEEM＊Abstract In this presentation, we report on de novo and axillary shoot regeneration and rooting of shoots maintained over a long term, from cultures of Tectona grandis L. Shoot-tips of te...Akram MUHAMMAD, Aftab FAHEEM＊Abstract In this presentation, we report on de novo and axillary shoot regeneration and rooting of shoots maintained over a long term, from cultures of Tectona grandis L. Shoot-tips of teak shoots forced from epicormic buds were used as the starting material for axenie shoot-culture establishment. Long term maintenance of such axenic shoot cultures was carried out by regular sub-culturing on MS media supplemented with N6-benzyleadenine （BA, 8.8 μmol·L^-1） and indole-3-butyric acid （IBA, 2 μmol·L ^1） for 24 months. Vigorously growing shoot tips （2-3 cm long） were inoculated on the MS basal medium supplemented with different concentrations （0, 1, 2, 4, 6, 8 or 10 p.mol-L-~） of either [BA or a-naphthaleneacetic acid （NAA） for rooting. Axillary and de novo shoots were de- veloped from axillary and cut basal ends of shoots, respectively. Shoots growing on auxins were further sub-cultured （every 15 days） and maintained for 45 days. The greatest number of de novo （5.06） as well as axillary shoots （2.85） was observed on the MS medium supplemented with 10 μmol-L^-1 NAA or 8 μmol·L^-1 IBA, respectively, after 45 days. The combinations of both IBA （μmol·L^-1） ＋ NAA （μmol·L^-1） were tested at different concentrations （4 ＋ 4, 6 ＋ 6, 8 ＋ 8） supplemented to a half strength MS basal medium with 0.1% activated charcoal for rooting of decapitated and non-decapitated de novo and axillary shoots. Rooting from non-decapitated de novo shoots was highest （93.33%） with a mean number of roots of 4.61 on this medium, supplemented with 6 μmol·L^-1 IBA ＋ 6 gmol.L l NAA, after 36 days of initial culture. Individual auxin, however, was not effective for root induction. Rooted shoots were acclimatized in a green house and after four weeks plantlets were transferred to the field.展开更多
Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in t...Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak （Tectona grand＆ L.）. Higher number of shoots （6.17） per log was produced under natural light as compared to shade conditions. Forcing was also better in natural light as compared to shade in terms of shoot length, number of nodes or leaves. For rooting, 2-4 cm long softwood shoots were excised and treated with either indole-3-butyric acid （IBA） or α-naphthyl acetic acid （NAA） at 0, 1000, 2000 or 3000 μmol.L^-1 each or with combinations （1000 ＋ 1000, 2000 ＋ 2000 or 3000 ＋ 3000 μmol.L^-1） and then placed in flat trays containing autoclaved sand at 25 ± 2℃ in 16 h photoperiod at 35 μmol.m^-2.s^-1. After 28 days, softwood cuttings treated with IBA ＋ NAA （3000 ＋ 3000 μmol.L^-1） had highest rooting percentage （89.3%） with 5.5 mean roots. Shoot apex and nodal explants of softwood cuttings were pretreated with 0.1% （w/v） ascorbic acid, boric acid, activated charcoal, citric acid, glutamine or polyvinylpolypyrollidone （PVP） for 24 h to remove phenolic compounds before surface disinfestation. Glutamine （G1） and PVP were equally effective resulting in 60% establishment of shoot apices on MS medium supplemented with 10 μmol.L^-1 6-benzylaminopurine （BAP） ＋ 5 μmol.L^-1 NAA. Using shoot apices, highest （42.80） number of multiple shoots with 54.33 mm shoot length were obtained on MS ＋ BAP （8.8 p.mol.L 1） ＋ IBA （2 μmol.L^-1） after 45 days. Shoots were successfully rooted and acclimatized to greenhouse conditions.展开更多
基金教育部重点项目，Application Fund of Agricultural Research Production
文摘Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concen- trations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8.
基金the provision of funds in the form of a research project(No.20-1155/R&D/07)awarded to FA
文摘Akram MUHAMMAD, Aftab FAHEEM＊Abstract In this presentation, we report on de novo and axillary shoot regeneration and rooting of shoots maintained over a long term, from cultures of Tectona grandis L. Shoot-tips of teak shoots forced from epicormic buds were used as the starting material for axenie shoot-culture establishment. Long term maintenance of such axenic shoot cultures was carried out by regular sub-culturing on MS media supplemented with N6-benzyleadenine （BA, 8.8 μmol·L^-1） and indole-3-butyric acid （IBA, 2 μmol·L ^1） for 24 months. Vigorously growing shoot tips （2-3 cm long） were inoculated on the MS basal medium supplemented with different concentrations （0, 1, 2, 4, 6, 8 or 10 p.mol-L-~） of either [BA or a-naphthaleneacetic acid （NAA） for rooting. Axillary and de novo shoots were de- veloped from axillary and cut basal ends of shoots, respectively. Shoots growing on auxins were further sub-cultured （every 15 days） and maintained for 45 days. The greatest number of de novo （5.06） as well as axillary shoots （2.85） was observed on the MS medium supplemented with 10 μmol-L^-1 NAA or 8 μmol·L^-1 IBA, respectively, after 45 days. The combinations of both IBA （μmol·L^-1） ＋ NAA （μmol·L^-1） were tested at different concentrations （4 ＋ 4, 6 ＋ 6, 8 ＋ 8） supplemented to a half strength MS basal medium with 0.1% activated charcoal for rooting of decapitated and non-decapitated de novo and axillary shoots. Rooting from non-decapitated de novo shoots was highest （93.33%） with a mean number of roots of 4.61 on this medium, supplemented with 6 μmol·L^-1 IBA ＋ 6 gmol.L l NAA, after 36 days of initial culture. Individual auxin, however, was not effective for root induction. Rooted shoots were acclimatized in a green house and after four weeks plantlets were transferred to the field.
文摘Softwood shoots were produced from 40 cm long stem segments placed horizontally in flat trays containing sterilized sand under natural light or shade conditions for subsequent rooting and micropropagation studies in teak （Tectona grand＆ L.）. Higher number of shoots （6.17） per log was produced under natural light as compared to shade conditions. Forcing was also better in natural light as compared to shade in terms of shoot length, number of nodes or leaves. For rooting, 2-4 cm long softwood shoots were excised and treated with either indole-3-butyric acid （IBA） or α-naphthyl acetic acid （NAA） at 0, 1000, 2000 or 3000 μmol.L^-1 each or with combinations （1000 ＋ 1000, 2000 ＋ 2000 or 3000 ＋ 3000 μmol.L^-1） and then placed in flat trays containing autoclaved sand at 25 ± 2℃ in 16 h photoperiod at 35 μmol.m^-2.s^-1. After 28 days, softwood cuttings treated with IBA ＋ NAA （3000 ＋ 3000 μmol.L^-1） had highest rooting percentage （89.3%） with 5.5 mean roots. Shoot apex and nodal explants of softwood cuttings were pretreated with 0.1% （w/v） ascorbic acid, boric acid, activated charcoal, citric acid, glutamine or polyvinylpolypyrollidone （PVP） for 24 h to remove phenolic compounds before surface disinfestation. Glutamine （G1） and PVP were equally effective resulting in 60% establishment of shoot apices on MS medium supplemented with 10 μmol.L^-1 6-benzylaminopurine （BAP） ＋ 5 μmol.L^-1 NAA. Using shoot apices, highest （42.80） number of multiple shoots with 54.33 mm shoot length were obtained on MS ＋ BAP （8.8 p.mol.L 1） ＋ IBA （2 μmol.L^-1） after 45 days. Shoots were successfully rooted and acclimatized to greenhouse conditions.
