An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By...An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.展开更多
Based on the field hyperspectral data from the analytical spectral devices （ASD） spectrometer, we characterized the spectral properties of rice canopies infested with brown spot disease and selected spectral regions...Based on the field hyperspectral data from the analytical spectral devices （ASD） spectrometer, we characterized the spectral properties of rice canopies infested with brown spot disease and selected spectral regions and bands sensitive to four severity degrees （severe, moderate, light, and healthy）. The results show that the curves＇ variation on the original and the first- and second-order de- rivative curves are greatly different, but the spectral difference in the near-infrared region is the most obvious for each level. Specifically, the peaks are located at 822, 738, and 793 nm, while the valleys are located at 402, 570, and 753 run, respectively. The sensitive regions are between 430-520, 530-550, and 650-710 nm, and the bands are 498, 539, and 673 nm in the sensitivity analysis, while they are in the ranges of 401-530, 550-730 as well as at 498 nm and 678 nm in the continuum removal.展开更多
目的利用Box-Behnken响应面设计法结合熵权逼近理想解排序(techniquefororderpreferenceby similarity to an ideal solution,TOPSIS)法优选松花粉米酒发酵工艺。方法以松花粉和糯米为主要原料,以感官评分、酒精度和抗氧化性为评价指标...目的利用Box-Behnken响应面设计法结合熵权逼近理想解排序(techniquefororderpreferenceby similarity to an ideal solution,TOPSIS)法优选松花粉米酒发酵工艺。方法以松花粉和糯米为主要原料,以感官评分、酒精度和抗氧化性为评价指标,在单因素试验的基础上,以松花粉添加量、酒曲添加量、发酵时间为考察因素,依据Box-Benhnken中心组合试验原理,设计3因素3水平响应面试验,结合熵权TOPSIS法优选松花粉米酒发酵工艺。结果松花粉米酒的最佳发酵工艺为松花粉添加量8%、酒曲添加量0.8%、发酵时间72h。在此条件下,松花粉米酒质地均一、口感柔和、呈现浅棕黄色。经过3批工艺验证试验测得米酒的感官评分为89.5分,酒精度为17.2%vol,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为87.43%。结论熵权TOPSIS法结合响应面设计优选松花粉米酒发酵工艺方法稳定,预测性较好,为生产高质量的松花粉米酒奠定了理论基础。展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.31860416 and 31460387)the Natural Science Foundation of Guangxi Province of China(Grant Nos.2015GXNS FAA139083,2016GXNSFAA380032 and 2017GXNS FAA198314)the Key Project of Science and Technology of Guangxi Province of China(Grant Nos.Guike AA17204070,AB16380079 and AB16380093)。
文摘An introgression line RBPH660,derived from wild rice Oryza rufipogon,showed stable resistance to brown planthopper(BPH).Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs.By using the bulked segregant analysis(BSA)-seq method,two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660,respectively.A major resistance locus,designated as Bph35 accounting for 51.27%of the phenotypic variation with a LOD score of 42.51,was mapped to the candidate region of chromosome 4 between In Del(insertion-deletion)markers PSM16 and R4 M13.For fine mapping of Bph35,one simple sequence repeat and three newly developed In Del markers were used to screen the recombinants.Finally,the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism(SNP)variant sites between the resistant and susceptible parents.Out of these genes,Os04 g0193950,encoding a putative NB-ARC(nucleotidebinding adaptor shared by APAF-1,R proteins and CED-4)and LRR(leucine-rich repeat)domain protein with nine non-synonymous SNP substitutions in its coding sequence regions,might be the candidate gene for Bph35.These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.
基金Supported by the National Natural Science Foundation of China (41071276 and 41101395)China Postdoctoral Science Foundation (20110490317)Postdoctoral Science Foundation of Beijing Academy of Agriculture and Forestry Sciences (2011)
文摘Based on the field hyperspectral data from the analytical spectral devices （ASD） spectrometer, we characterized the spectral properties of rice canopies infested with brown spot disease and selected spectral regions and bands sensitive to four severity degrees （severe, moderate, light, and healthy）. The results show that the curves＇ variation on the original and the first- and second-order de- rivative curves are greatly different, but the spectral difference in the near-infrared region is the most obvious for each level. Specifically, the peaks are located at 822, 738, and 793 nm, while the valleys are located at 402, 570, and 753 run, respectively. The sensitive regions are between 430-520, 530-550, and 650-710 nm, and the bands are 498, 539, and 673 nm in the sensitivity analysis, while they are in the ranges of 401-530, 550-730 as well as at 498 nm and 678 nm in the continuum removal.
文摘目的利用Box-Behnken响应面设计法结合熵权逼近理想解排序(techniquefororderpreferenceby similarity to an ideal solution,TOPSIS)法优选松花粉米酒发酵工艺。方法以松花粉和糯米为主要原料,以感官评分、酒精度和抗氧化性为评价指标,在单因素试验的基础上,以松花粉添加量、酒曲添加量、发酵时间为考察因素,依据Box-Benhnken中心组合试验原理,设计3因素3水平响应面试验,结合熵权TOPSIS法优选松花粉米酒发酵工艺。结果松花粉米酒的最佳发酵工艺为松花粉添加量8%、酒曲添加量0.8%、发酵时间72h。在此条件下,松花粉米酒质地均一、口感柔和、呈现浅棕黄色。经过3批工艺验证试验测得米酒的感官评分为89.5分,酒精度为17.2%vol,1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为87.43%。结论熵权TOPSIS法结合响应面设计优选松花粉米酒发酵工艺方法稳定,预测性较好,为生产高质量的松花粉米酒奠定了理论基础。
