Obesity has become a global threat to health;however,the available drugs for treating obesity are limited.We investigated the anti-obesity effect of hydroxy-α-sanshool(HAS),an amide derived from the fruit of Zanthoxy...Obesity has become a global threat to health;however,the available drugs for treating obesity are limited.We investigated the anti-obesity effect of hydroxy-α-sanshool(HAS),an amide derived from the fruit of Zanthoxylum bungeanum,which promotes the management of obesity by triggering the browning of white adipose tissue(WAT)targeting the membrane receptor of transient receptor potential vanilloid 1(TRPV1).However,HAS easily undergoes configuration transformation and oxidative degradation.The short peptide CKGGRAKDC or adipose-targeting sequence(ATS)binds specifically to prohibitin on the surface of WAT cells and can be used as recognition assembly to enhance adipocyte targetability.Furthermore,mesoporous silica nanoparticles(MSNs)are widely used in drug delivery systems because of their large specific surface area and pore volume.Therefore,HAS-loaded adipose-targeted MSNs(MSNs-ATS)were developed to enhance the adipocyte targetability,safety,and efficacy of HAS,and tested on mature 3T3-L1 cells and obese mouse models.MSNs-ATS showed higher specificity for adipocyte targetability without obvious toxicity.HAS-loaded MSNs-ATS showed anti-obesity effects superior to those of HAS alone.In conclusion,we successfully developed adipocyte-targeted,HAS-loaded MSNs with good safety and anti-obesity effects.展开更多
This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a ...This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a brief analysis of the challenges encountered in the study of tobacco brown spot,which include the study of pathogenesis and virulence,the breeding of disease-resistant varieties,the screening of low-toxicity and high-efficiency agents,the development of biological control methods with more stable efficacy,and the necessity for accurate prediction and forecasting techniques.展开更多
Brown planthopper(BPH)is a highly destructive pest that presents a significant challenge to rice production,particularly in the Asia-Pacific region.Numerous BPH-resistant rice varieties have been successfully bred and...Brown planthopper(BPH)is a highly destructive pest that presents a significant challenge to rice production,particularly in the Asia-Pacific region.Numerous BPH-resistant rice varieties have been successfully bred and released for commercial cultivation across diverse rice-growing ecosystems.However,resistance breakdown in several varieties carrying major resistance genes has been reported,highlighting the urgent need for the development of novel,genetically diverse,and broad-spectrum resistant varieties.To date,more than 45 resistance loci have been identified and mapped from both cultivated and wild rice species.Among these,a subset of genes(including Bph1,Bph3,Bph6,Bph7,Bph9,Bph10,Bph14,Bph15,Bph18,Bph21,Bph26/2,bph29,Bph32,Bph37,and Bph30/Bph40)have been positionally cloned.Most of these genes encode coiled-coil nucleotide-binding leucine-rich repeat proteins,which are central to plant immune responses,along with a few signaling molecules playing pivotal roles.In addition to these core resistance genes,various other genetic components,including miRNAs,protein kinases,and transcription factors,have been functionally characterized for their roles in mediating BPH resistance.The advent of post-genomic tools such as RNA sequencing and single-cell sequencing,along with cutting-edge genomic technologies like CRISPR/Cas gene editing,has significantly accelerated resistance breeding programs.In this context,we provide an overview of genetics,mapping,isolation,and functional characterization of BPH resistance,along with strategies for incorporating resistance using advanced genomics-assisted breeding tools.Furthermore,we present a snapshot of how the integration of genomics and novel breeding technologies holds great promise for dissecting the genetic architecture of pest resistance and accelerating crop improvement.展开更多
Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuo...Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuous light exposure.This study aimed to investigate the morphological and functional alterations in BAT under continuous light conditions and to identify traditional Chinese medicine compounds capable of reversing these changes.Methods:A metabolic disorder model was established by subjecting mice to continuous light exposure for 5 weeks.During this period,body weight,food intake,and body fat percentage were monitored.Serum levels of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)were measured to assess lipid metabolism.Histological changes in BAT were examined using H&E staining.The expression of the thermogenic marker uncoupling protein 1(UCP1)in BAT was determined by RT-qPCR and Western blot to evaluate thermogenic function.RNA sequencing(RNA-seq)was employed to identify differentially expressed genes(DEGs)involved in BAT whitening induced by prolonged continuous light exposure.DEGs were analyzed using the connectivity map(CMap)database to identify potential preventive and therapeutic compounds.The therapeutic efficacy of the selected compounds was subsequently evaluated using the above indicators,and key pathways were validated through western blot analysis.Results:After 5 weeks of continuous light exposure,mice exhibited increased body fat percentage and serum levels of TG,impaired mitochondrial function,reduced thermogenic capacity,and whitening of BAT.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses indicated that BAT whitening was primarily associated with the adenosine 5'-monophosphate-activated protein kinase(AMPK)signaling pathway,fatty acid metabolism,and circadian rhythm.Ten hub genes identified using Cytoscape were mainly related to AMPK signaling and heat shock proteins.In vivo experiments showed that cordycepin significantly attenuated the increase in body fat percentage caused by prolonged light exposure.This effect was mediated by activation of the AMPK/PGC-1α/UCP1 signaling pathway,which restored the multilocular morphology and thermogenic function of BAT.Conclusion:Cordycepin mitigates continuous light-induced BAT whitening and metabolic disturbances by activating the AMPK signaling pathway.展开更多
During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many...During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many edible plants,can ameliorate obesity by activating adipose tissue browning,but its effect on circadian clock in this process remains poorly understood.Here we found that dietary LU improved circadian misalignment of energy expenditure in high-fat diet(HFD)-fed wild-type(WT)mice.Moreover,dietary LU efficiently elevated uncoupling protein 1 levels in adipose tissue during the dark period,which was similar to the LU-increased hepatic PER2 expressions.Hepatic peroxisome proliferators-activated receptorsα(PPARα)/recombinant retinoid X receptorα(RXRα)/fibroblast growth factor 21(FGF21)pathway was rhythmically elevated by dietary LU in HFD-fed WT mice,whereas the promotion was inhibited in Per2^(-/-)mice.Meanwhile,Per2 deletion abolished the effects of dietary LU on adipose tissue browning in HFD-fed mice.Further,LU treatment directly activated PPARα/RXRα/FGF21 signaling in primary cultured hepatocytes from WT mice rather than Per2^(-/-)mice.Taken together,the deletion of the core clock component Per2 impedes LUinduced adipose tissue browning through weakening PPARα/RXRα/FGF21 pathway in mice,providing a new insight into the interplay of energy metabolism and circadian clock for the anti-obesity activity of LU.展开更多
Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism...Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism in adipose tissue,and modulate the composition of gut microbiota in animal fed high-fat diet(HFD).However,the role of gut microbiota in the improvement of obesity by PPPs has not been elucidated.In current study,we applied antibiotics to inhibit gut microbiota in mice fed HFD and treated with PPPs.The results showed that the inhibition of gut microbiota impair the effect of PPPs on reducing obesity and promoting adipose browning,and change the fecal metabolomic profiles in respond to PPPs.Moreover,the inhibition of gut microbiota supressed the promotive effects of PPPs on the levels of Akkermansia and microbiota-related metabolites,such as urolithin A,short-chain fatty acids(SCFAs),and bile acids(BAs),which were associated with activating adipose browning.Therefore,our results suggested that the presence of gut microbiota is essential for PPPs to ameliorate HFD-induced obesity.The related bacteria or metabolites generated by the interaction between PPPs and microbiota promote adipose browning and facilitate the beneficial effects of PPPs.展开更多
Brown carbon(BrC)has attracted widespread attention because of its strong absorption of solar radiation in the ultraviolet-visible wavelength range,which causes adverse impacts on human health.Originally,BrC was a phy...Brown carbon(BrC)has attracted widespread attention because of its strong absorption of solar radiation in the ultraviolet-visible wavelength range,which causes adverse impacts on human health.Originally,BrC was a physically defined class of substances.However,current research has gradually shifted towards the identification of its chemical groups,because its light-absorbing capability,chemical properties and health effects mainly depend on the chemical composition of its chromophores.Therefore,this review mainly focuses on the chemical understanding of BrC based on chromophores,and the secondary formation mechanism of chromophores,photosensitized reactions,and human health effects of BrC were detailly summarized.Firstly,BrC chromophores are divided into five categories:nitrogen-heterocycles,nitrogen-chain,aromatic species,oligomers and sulfur-containing organic compounds.Different chromophore precursor species exhibit variations,and their formation mechanisms are also distinct.Secondly,BrC can trigger the production of secondary organic aerosol(SOA)precursors or cause SOA growth because BrC is an important component of light-absorbing particles formed during incomplete combustion of biomass and fossil fuels,potentially exerting adverse effects on human health.Finally,developing sufficiently separated methods for BrC and refining algorithms and machine learning can lead to a more effective understanding of the chemical composition of chromophores,thus enabling better evaluation of the atmospheric effects and health impacts of BrC.In all,this review provides new insights into the categories of BrC chromophores and new advance in secondary formation mechanisms,photosensitized reactions,and human health effects on the basis of chemical structures.展开更多
[Objective]The paper was to investigate the effects of substituting corn pellets with potato residue on the growth performance,rumen fermentation indicators and economic benefits of Xinjiang Brown cattle.[Method]A tot...[Objective]The paper was to investigate the effects of substituting corn pellets with potato residue on the growth performance,rumen fermentation indicators and economic benefits of Xinjiang Brown cattle.[Method]A total of 80 Xinjiang Brown cattle,each 22 months old and with a similar body weight of(250±15)kg,were randomly assigned to four groups,with 20 cattle in each group,utilizing a single-factor experimental design.The total mixed diets for the experimental groups Ⅰ,Ⅱ,and Ⅲ were formulated by substituting corn pellets with 2.00%,4.00%,and 6.00%potato residue,respectively,while the diet in the control group was not substituted with potato residue.The duration of the test was 100 d,which comprised a pre-test period of 10 d and a formal test period of 90 d.During the test period,the average dry matter intake,average daily weight gain and feed-gain ratio were assessed at 30-day intervals,and body size indicators were measured on days 0 and 90.Rumen fluid samples were collected from the oral cavity on days 0,30,60,and 90 to assess rumen fermentation parameters.Additionally,jugular blood samples were obtained during the fasting period,and serum was separated for the analysis of total protein mass concentration,urea nitrogen,and glucose concentration.Furthermore,the economic benefits accrued during the test period were calculated.[Result]The dry matter intake in experimental group Ⅰ was significantly higher than that in the control group from days 31 to 60(P<0.05).In contrast,the differences in dry matter intake between experimental groups Ⅱ and Ⅲ and the control group were not statistically significant(P>0.05).From days 61 to 90,the dry matter intake in experimental group Ⅰ remained significantly higher than that in the other groups(P<0.05).Throughout the entire experimental period,the average daily weight gain observed in experimental group Ⅰ was significantly greater than that recorded in the other groups(P<0.05).Additionally,the total weight gain over the entire period was significantly higher than that of experimental group Ⅱ(P<0.05)and markedly higher than that of both the control group and experimental group Ⅲ(P<0.01).The feed-gain ratio in experimental group Ⅰ was significantly lower than that in experimental group Ⅱ(P<0.05).There were no statisticaly significant differences observed in body height,slope length,thoracic circumference,abdominal circumference,rumen pH,acetic acid,propionic acid,butyric acid,valeric acid,total volatile fatty acids,and ammoniacal nitrogen mass concentration among the groups(P>0.05).On the 90th day of the experiment,the serum urea nitrogen concentration in beef cattle from experimental groups Ⅰ and Ⅱ was significantly lower than that in the control group and experimental group Ⅲ(P<0.05).Additionally,the profit margins in experimental groups Ⅰ,Ⅱ,and Ⅲ were higher than those in the control group,and the profit margin in experimental group Ⅰ was the highest.[Conclusion]The substitution of corn pellets with potato residue has the potential to enhance the average daily weight gain and total weight gain throughout the entire period in beef cattle,while simultaneously reducing the feed-gain ratio.However,this substitution does not appear to have a significant impact on body size indicators,rumen fermentation parameters,or the concentrations of serum total protein and glucose in beef cattle.Furthermore,this approach may yield improved breeding profitability,with a recommended substitution ratio of 2.00%based on the conditions of this experiment.展开更多
Against the backdrop of intangible cultural heritage(ICH)activation in China,the innovative transmission and industrial integration of ICH have become increasingly significant.As an officially recognized ICH item of Z...Against the backdrop of intangible cultural heritage(ICH)activation in China,the innovative transmission and industrial integration of ICH have become increasingly significant.As an officially recognized ICH item of Zhejiang Province,Yiwu brown sugar embodies unique artisanal techniques and carries the cultural memory of Yiwu.However,it currently faces challenges such as discontinuity in transmission,insufficient market recognition,and inadequate industrial integration.Focusing on the construction of a youth-oriented IP system for Yiwu brown sugar within the framework of ICH activation,this study employs field investigations and literature research to excavate the historical,cultural,and craft-related foundations of Yiwu brown sugar.It proposes strategies for developing a youthful IP identity through visual system innovation,digital content creation,and social-media-based dissemination,and explores integrated development pathways across culinary innovation,cultural and creative industries,and digital marketing.The aim is to provide theoretical support and practical guidance for the activation of Yiwu brown sugar as intangible cultural heritage.展开更多
Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent chall...Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent challenge in rice breeding programs.Notably,compared with GW,brown rice weight(BRW)provides a more direct metric associated with actual grain yield potential.In this study,we conducted a two-year replicated genome-wide association study to elucidate the genetic architecture of BRW and identify new loci regulating GW.Among seven consistently detected loci across experimental replicates,four were not co-localized with previously reported genes associated with BRW or GW traits.BRW1.1,one of the four newly identified loci,was found to encode a novel RNA-binding protein.Functional characterization revealed that BRW1.1 acts as a negative regulator of BRW,potentially through modulating mRNA translation processes.Intriguingly,through integrated analysis of mutant phenotypes and haplotype variations,we demonstrated that BRW1.1 mediates the physiological tradeoff between GW and panicle number.This study not only delineates the genetic determinants of BRW but also identifies BRW1.1 as a promising molecular target for breaking the yield component tradeoff in precision rice breeding.展开更多
基金supported by the Natural Science Foundation of Sichuan Province(No.2022NSFSC0720)Research Center for the Development of the Comprehensive Health Industry and Rural Revitalization of Sichuan TCM(No.DJKYB202306)State Administration of Traditional Chinese Medicine of Sichuan Province of China(No.2020HJZX001).
文摘Obesity has become a global threat to health;however,the available drugs for treating obesity are limited.We investigated the anti-obesity effect of hydroxy-α-sanshool(HAS),an amide derived from the fruit of Zanthoxylum bungeanum,which promotes the management of obesity by triggering the browning of white adipose tissue(WAT)targeting the membrane receptor of transient receptor potential vanilloid 1(TRPV1).However,HAS easily undergoes configuration transformation and oxidative degradation.The short peptide CKGGRAKDC or adipose-targeting sequence(ATS)binds specifically to prohibitin on the surface of WAT cells and can be used as recognition assembly to enhance adipocyte targetability.Furthermore,mesoporous silica nanoparticles(MSNs)are widely used in drug delivery systems because of their large specific surface area and pore volume.Therefore,HAS-loaded adipose-targeted MSNs(MSNs-ATS)were developed to enhance the adipocyte targetability,safety,and efficacy of HAS,and tested on mature 3T3-L1 cells and obese mouse models.MSNs-ATS showed higher specificity for adipocyte targetability without obvious toxicity.HAS-loaded MSNs-ATS showed anti-obesity effects superior to those of HAS alone.In conclusion,we successfully developed adipocyte-targeted,HAS-loaded MSNs with good safety and anti-obesity effects.
文摘This paper provide a comprehensive overview of research findings regarding the pathogen responsible for tobacco brown spot,its occurrence regularity,and integrated control strategies.Additionally,this study provide a brief analysis of the challenges encountered in the study of tobacco brown spot,which include the study of pathogenesis and virulence,the breeding of disease-resistant varieties,the screening of low-toxicity and high-efficiency agents,the development of biological control methods with more stable efficacy,and the necessity for accurate prediction and forecasting techniques.
文摘Brown planthopper(BPH)is a highly destructive pest that presents a significant challenge to rice production,particularly in the Asia-Pacific region.Numerous BPH-resistant rice varieties have been successfully bred and released for commercial cultivation across diverse rice-growing ecosystems.However,resistance breakdown in several varieties carrying major resistance genes has been reported,highlighting the urgent need for the development of novel,genetically diverse,and broad-spectrum resistant varieties.To date,more than 45 resistance loci have been identified and mapped from both cultivated and wild rice species.Among these,a subset of genes(including Bph1,Bph3,Bph6,Bph7,Bph9,Bph10,Bph14,Bph15,Bph18,Bph21,Bph26/2,bph29,Bph32,Bph37,and Bph30/Bph40)have been positionally cloned.Most of these genes encode coiled-coil nucleotide-binding leucine-rich repeat proteins,which are central to plant immune responses,along with a few signaling molecules playing pivotal roles.In addition to these core resistance genes,various other genetic components,including miRNAs,protein kinases,and transcription factors,have been functionally characterized for their roles in mediating BPH resistance.The advent of post-genomic tools such as RNA sequencing and single-cell sequencing,along with cutting-edge genomic technologies like CRISPR/Cas gene editing,has significantly accelerated resistance breeding programs.In this context,we provide an overview of genetics,mapping,isolation,and functional characterization of BPH resistance,along with strategies for incorporating resistance using advanced genomics-assisted breeding tools.Furthermore,we present a snapshot of how the integration of genomics and novel breeding technologies holds great promise for dissecting the genetic architecture of pest resistance and accelerating crop improvement.
文摘Background:Long-term exposure to light has emerged as a novel risk factor for metabolic diseases.The whitening of brown adipose tissue(BAT)may play an important role in metabolic disorders caused by long-term continuous light exposure.This study aimed to investigate the morphological and functional alterations in BAT under continuous light conditions and to identify traditional Chinese medicine compounds capable of reversing these changes.Methods:A metabolic disorder model was established by subjecting mice to continuous light exposure for 5 weeks.During this period,body weight,food intake,and body fat percentage were monitored.Serum levels of triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C),and low density lipoprotein cholesterol(LDL-C)were measured to assess lipid metabolism.Histological changes in BAT were examined using H&E staining.The expression of the thermogenic marker uncoupling protein 1(UCP1)in BAT was determined by RT-qPCR and Western blot to evaluate thermogenic function.RNA sequencing(RNA-seq)was employed to identify differentially expressed genes(DEGs)involved in BAT whitening induced by prolonged continuous light exposure.DEGs were analyzed using the connectivity map(CMap)database to identify potential preventive and therapeutic compounds.The therapeutic efficacy of the selected compounds was subsequently evaluated using the above indicators,and key pathways were validated through western blot analysis.Results:After 5 weeks of continuous light exposure,mice exhibited increased body fat percentage and serum levels of TG,impaired mitochondrial function,reduced thermogenic capacity,and whitening of BAT.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses indicated that BAT whitening was primarily associated with the adenosine 5'-monophosphate-activated protein kinase(AMPK)signaling pathway,fatty acid metabolism,and circadian rhythm.Ten hub genes identified using Cytoscape were mainly related to AMPK signaling and heat shock proteins.In vivo experiments showed that cordycepin significantly attenuated the increase in body fat percentage caused by prolonged light exposure.This effect was mediated by activation of the AMPK/PGC-1α/UCP1 signaling pathway,which restored the multilocular morphology and thermogenic function of BAT.Conclusion:Cordycepin mitigates continuous light-induced BAT whitening and metabolic disturbances by activating the AMPK signaling pathway.
文摘During the development of diet-induced obesity,the change of energy matebolism is closely related to the function of the circadian clock in mammals.Luteolin(LU),one of the most common natural flavonoids riched in many edible plants,can ameliorate obesity by activating adipose tissue browning,but its effect on circadian clock in this process remains poorly understood.Here we found that dietary LU improved circadian misalignment of energy expenditure in high-fat diet(HFD)-fed wild-type(WT)mice.Moreover,dietary LU efficiently elevated uncoupling protein 1 levels in adipose tissue during the dark period,which was similar to the LU-increased hepatic PER2 expressions.Hepatic peroxisome proliferators-activated receptorsα(PPARα)/recombinant retinoid X receptorα(RXRα)/fibroblast growth factor 21(FGF21)pathway was rhythmically elevated by dietary LU in HFD-fed WT mice,whereas the promotion was inhibited in Per2^(-/-)mice.Meanwhile,Per2 deletion abolished the effects of dietary LU on adipose tissue browning in HFD-fed mice.Further,LU treatment directly activated PPARα/RXRα/FGF21 signaling in primary cultured hepatocytes from WT mice rather than Per2^(-/-)mice.Taken together,the deletion of the core clock component Per2 impedes LUinduced adipose tissue browning through weakening PPARα/RXRα/FGF21 pathway in mice,providing a new insight into the interplay of energy metabolism and circadian clock for the anti-obesity activity of LU.
基金supported by the National Natural Science Foundation of China(32001679 and 31871801)the Science and Technology Research of Shaanxi Province(2021QFY07-03)+1 种基金supported by the Fundamental Research Funds for the Central Universities(GK202103098)the Scientific and Technological Achievements Commercialization Program of Shaanxi(2023-YDCGZH-13)。
文摘Gut microbiota regulate the activation of adipose browning,which promote energy dissipation and combat diet-induced obesity.Pomegranate peel polyphenols(PPPs)have been shown to reduce obesity,regulate lipid metabolism in adipose tissue,and modulate the composition of gut microbiota in animal fed high-fat diet(HFD).However,the role of gut microbiota in the improvement of obesity by PPPs has not been elucidated.In current study,we applied antibiotics to inhibit gut microbiota in mice fed HFD and treated with PPPs.The results showed that the inhibition of gut microbiota impair the effect of PPPs on reducing obesity and promoting adipose browning,and change the fecal metabolomic profiles in respond to PPPs.Moreover,the inhibition of gut microbiota supressed the promotive effects of PPPs on the levels of Akkermansia and microbiota-related metabolites,such as urolithin A,short-chain fatty acids(SCFAs),and bile acids(BAs),which were associated with activating adipose browning.Therefore,our results suggested that the presence of gut microbiota is essential for PPPs to ameliorate HFD-induced obesity.The related bacteria or metabolites generated by the interaction between PPPs and microbiota promote adipose browning and facilitate the beneficial effects of PPPs.
基金supported by the National Natural Science Foundation of China(Nos.42020104001,42327806 and 42177354).
文摘Brown carbon(BrC)has attracted widespread attention because of its strong absorption of solar radiation in the ultraviolet-visible wavelength range,which causes adverse impacts on human health.Originally,BrC was a physically defined class of substances.However,current research has gradually shifted towards the identification of its chemical groups,because its light-absorbing capability,chemical properties and health effects mainly depend on the chemical composition of its chromophores.Therefore,this review mainly focuses on the chemical understanding of BrC based on chromophores,and the secondary formation mechanism of chromophores,photosensitized reactions,and human health effects of BrC were detailly summarized.Firstly,BrC chromophores are divided into five categories:nitrogen-heterocycles,nitrogen-chain,aromatic species,oligomers and sulfur-containing organic compounds.Different chromophore precursor species exhibit variations,and their formation mechanisms are also distinct.Secondly,BrC can trigger the production of secondary organic aerosol(SOA)precursors or cause SOA growth because BrC is an important component of light-absorbing particles formed during incomplete combustion of biomass and fossil fuels,potentially exerting adverse effects on human health.Finally,developing sufficiently separated methods for BrC and refining algorithms and machine learning can lead to a more effective understanding of the chemical composition of chromophores,thus enabling better evaluation of the atmospheric effects and health impacts of BrC.In all,this review provides new insights into the categories of BrC chromophores and new advance in secondary formation mechanisms,photosensitized reactions,and human health effects on the basis of chemical structures.
基金Supported by Natural Science Foundation Key Project of Xinjiang Uygur Autonomous Region(2022D01D10)China Agricultural Industry Research System of Xinjiang Uygur Autonomous Region in 2024(XJARS-12-05)Project of Key Laboratory of Animal Nutrition and Feed of Yili Prefecture.
文摘[Objective]The paper was to investigate the effects of substituting corn pellets with potato residue on the growth performance,rumen fermentation indicators and economic benefits of Xinjiang Brown cattle.[Method]A total of 80 Xinjiang Brown cattle,each 22 months old and with a similar body weight of(250±15)kg,were randomly assigned to four groups,with 20 cattle in each group,utilizing a single-factor experimental design.The total mixed diets for the experimental groups Ⅰ,Ⅱ,and Ⅲ were formulated by substituting corn pellets with 2.00%,4.00%,and 6.00%potato residue,respectively,while the diet in the control group was not substituted with potato residue.The duration of the test was 100 d,which comprised a pre-test period of 10 d and a formal test period of 90 d.During the test period,the average dry matter intake,average daily weight gain and feed-gain ratio were assessed at 30-day intervals,and body size indicators were measured on days 0 and 90.Rumen fluid samples were collected from the oral cavity on days 0,30,60,and 90 to assess rumen fermentation parameters.Additionally,jugular blood samples were obtained during the fasting period,and serum was separated for the analysis of total protein mass concentration,urea nitrogen,and glucose concentration.Furthermore,the economic benefits accrued during the test period were calculated.[Result]The dry matter intake in experimental group Ⅰ was significantly higher than that in the control group from days 31 to 60(P<0.05).In contrast,the differences in dry matter intake between experimental groups Ⅱ and Ⅲ and the control group were not statistically significant(P>0.05).From days 61 to 90,the dry matter intake in experimental group Ⅰ remained significantly higher than that in the other groups(P<0.05).Throughout the entire experimental period,the average daily weight gain observed in experimental group Ⅰ was significantly greater than that recorded in the other groups(P<0.05).Additionally,the total weight gain over the entire period was significantly higher than that of experimental group Ⅱ(P<0.05)and markedly higher than that of both the control group and experimental group Ⅲ(P<0.01).The feed-gain ratio in experimental group Ⅰ was significantly lower than that in experimental group Ⅱ(P<0.05).There were no statisticaly significant differences observed in body height,slope length,thoracic circumference,abdominal circumference,rumen pH,acetic acid,propionic acid,butyric acid,valeric acid,total volatile fatty acids,and ammoniacal nitrogen mass concentration among the groups(P>0.05).On the 90th day of the experiment,the serum urea nitrogen concentration in beef cattle from experimental groups Ⅰ and Ⅱ was significantly lower than that in the control group and experimental group Ⅲ(P<0.05).Additionally,the profit margins in experimental groups Ⅰ,Ⅱ,and Ⅲ were higher than those in the control group,and the profit margin in experimental group Ⅰ was the highest.[Conclusion]The substitution of corn pellets with potato residue has the potential to enhance the average daily weight gain and total weight gain throughout the entire period in beef cattle,while simultaneously reducing the feed-gain ratio.However,this substitution does not appear to have a significant impact on body size indicators,rumen fermentation parameters,or the concentrations of serum total protein and glucose in beef cattle.Furthermore,this approach may yield improved breeding profitability,with a recommended substitution ratio of 2.00%based on the conditions of this experiment.
基金Supported by the 2025 National Undergraduate Innovation and Entrepreneurship Training Program(Project No.202511057057).
文摘Against the backdrop of intangible cultural heritage(ICH)activation in China,the innovative transmission and industrial integration of ICH have become increasingly significant.As an officially recognized ICH item of Zhejiang Province,Yiwu brown sugar embodies unique artisanal techniques and carries the cultural memory of Yiwu.However,it currently faces challenges such as discontinuity in transmission,insufficient market recognition,and inadequate industrial integration.Focusing on the construction of a youth-oriented IP system for Yiwu brown sugar within the framework of ICH activation,this study employs field investigations and literature research to excavate the historical,cultural,and craft-related foundations of Yiwu brown sugar.It proposes strategies for developing a youthful IP identity through visual system innovation,digital content creation,and social-media-based dissemination,and explores integrated development pathways across culinary innovation,cultural and creative industries,and digital marketing.The aim is to provide theoretical support and practical guidance for the activation of Yiwu brown sugar as intangible cultural heritage.
基金supported by the National Natural Science Foundation of China(Grant Nos.32000377,32172037,and 32472211)the Biological Breeding-National Science and Technology Major Project,China(Grant No.2023ZD04068)+2 种基金the Fundamental Research Funds for the Central Universities,China(Grant No.KJQN202103)the open funds of the State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization,China(Grant No.ZW202401)the Cyrus Tang Innovation Center for Crop Seed Industry,China.
文摘Rice grain yield is primarily determined by three key agronomic traits:panicle number,grain number per panicle,and grain weight(GW).However,the inherent tradeoffs among these yield components remain a persistent challenge in rice breeding programs.Notably,compared with GW,brown rice weight(BRW)provides a more direct metric associated with actual grain yield potential.In this study,we conducted a two-year replicated genome-wide association study to elucidate the genetic architecture of BRW and identify new loci regulating GW.Among seven consistently detected loci across experimental replicates,four were not co-localized with previously reported genes associated with BRW or GW traits.BRW1.1,one of the four newly identified loci,was found to encode a novel RNA-binding protein.Functional characterization revealed that BRW1.1 acts as a negative regulator of BRW,potentially through modulating mRNA translation processes.Intriguingly,through integrated analysis of mutant phenotypes and haplotype variations,we demonstrated that BRW1.1 mediates the physiological tradeoff between GW and panicle number.This study not only delineates the genetic determinants of BRW but also identifies BRW1.1 as a promising molecular target for breaking the yield component tradeoff in precision rice breeding.
