Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol/L NaC l,to investigate the effects of salt stress on the physiological phenotype and antioxidant enzyme act...Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol/L NaC l,to investigate the effects of salt stress on the physiological phenotype and antioxidant enzyme activity of S.breviflora seedlings.The results showed the germination rate,root length and fresh weight of seedlings were all influenced by salinity.Germination rate,root length and fresh weight of seedlings were significantly inhibited by 100,150 and50 mmol/L NaC l,and were 62%,1.30 cm,0.299 g,respectively.With NaC l content increasing,the trend of superoxide dismutase( SOD) and peroxidase( POD) activity was rising at first and then falling,and the activity of SOD and POD reached the maximum at 150 and 100 mmol/L,48.6 and 6 766 U/g,respectively.The activity of catalase( CAT) continued to increase and reached the maximum at 150 mmol/L,299 U/g.The isozyme zymogram showed that SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of S.breviflora seedlings.展开更多
Background:Long-term overgrazing has led to severe degradation of grasslands,posing a significant threat to the sustainable use of grassland resources.Methods:Based on the investigation of changes in functional traits...Background:Long-term overgrazing has led to severe degradation of grasslands,posing a significant threat to the sustainable use of grassland resources.Methods:Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing,moderate grazing,and heavy grazing treatments,the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses.Results:Differentially expressed genes and proteins were identified under different grazing intensities.They were mainly related to RNA processing,carbon metabolism,and secondary metabolite biosynthesis.These findings suggest that long-term grazing leads to molecular phenotypic plasticity,affecting various biological processes and metabolic pathways in S.breviflora.Correlation analysis revealed low correlation between the transcriptome and the proteome,indicating a large-scale regulation of gene expression at the posttranscriptional and translational levels during the response of S.breviflora to grazing.The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S.breviflora.Conclusions:Our study provides insight into the adaptation mechanisms of S.breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices.展开更多
基金Supported by Introduced Personnel Research Start-up Fund of North Minzu University(4400302550)National Natural Science Foundation of China(31600202)
文摘Stipa breviflora seeds,collected from Inner Mongolia,were sowed on MS medium containing 0,50,100,150,200 mmol/L NaC l,to investigate the effects of salt stress on the physiological phenotype and antioxidant enzyme activity of S.breviflora seedlings.The results showed the germination rate,root length and fresh weight of seedlings were all influenced by salinity.Germination rate,root length and fresh weight of seedlings were significantly inhibited by 100,150 and50 mmol/L NaC l,and were 62%,1.30 cm,0.299 g,respectively.With NaC l content increasing,the trend of superoxide dismutase( SOD) and peroxidase( POD) activity was rising at first and then falling,and the activity of SOD and POD reached the maximum at 150 and 100 mmol/L,48.6 and 6 766 U/g,respectively.The activity of catalase( CAT) continued to increase and reached the maximum at 150 mmol/L,299 U/g.The isozyme zymogram showed that SOD 2-4,POD 1-2,and CAT 1-2 were positively correlated with salinity tolerance of S.breviflora seedlings.
基金National Natural Science Foundation of China,Grant/Award Number:32160088Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region of China,Grant/Award Number:NJYT22093Open Project Program of Ministry of Education Key Laboratory of Ecology and Resources Use of the Mongolian Plateau。
文摘Background:Long-term overgrazing has led to severe degradation of grasslands,posing a significant threat to the sustainable use of grassland resources.Methods:Based on the investigation of changes in functional traits and photosynthetic physiology of Stipa breviflora under no grazing,moderate grazing,and heavy grazing treatments,the changes in expression patterns of genes and proteins associated with different grazing intensities were assessed through integrative transcriptomic and proteomic analyses.Results:Differentially expressed genes and proteins were identified under different grazing intensities.They were mainly related to RNA processing,carbon metabolism,and secondary metabolite biosynthesis.These findings suggest that long-term grazing leads to molecular phenotypic plasticity,affecting various biological processes and metabolic pathways in S.breviflora.Correlation analysis revealed low correlation between the transcriptome and the proteome,indicating a large-scale regulation of gene expression at the posttranscriptional and translational levels during the response of S.breviflora to grazing.The expression profiles of key genes and proteins involved in photosynthesis and phenylpropanoid metabolism pathways suggested their synergistic response to grazing in S.breviflora.Conclusions:Our study provides insight into the adaptation mechanisms of S.breviflora to grazing and provides a scientific basis for the development of more efficient grassland protection and utilization practices.
