Background:Trichoderma brevicompactum can control of plant diseases,but there are few studies about the control of root rot by T.brevicompactum 31636 and its biocontrol mechanism.Objective:To explore the impact of T.b...Background:Trichoderma brevicompactum can control of plant diseases,but there are few studies about the control of root rot by T.brevicompactum 31636 and its biocontrol mechanism.Objective:To explore the impact of T.brevicompactum 31636 on root rot and the structure of fungal communities of rhizosphere soil.Methods:The effect of T.brevicompactum 31636 on controlling root rot was assessed,the growth of plants was observed by pot experiments,and the fungal structure was detected by high-throughput sequencing analysis.Results:Compared with the CK(healthy plants)group,the plant height,fresh weight,and dry weight increased by 55.3%,48.55%and 57.8%,respectively.While the concentration of atractylenolideⅠ,Ⅱ,Ⅲ,and atractylone increased by 19.35%,16.96%,89.42%and 89.32%,respectively.The results of pot experiments indicated that T.brevicompactum 31636 could significantly improve the survival rate of Atractylodes macrocephala.The results of principal coordinates analysis(PCoA)showed that the sample of CK and Fo+Th(adding Fusdrium oxysporum before T.brevicompactum 31636)group on one side,and samples of Th and Fo group on the other.Furthermore,among the top 20 dominant fungi detected,the fungal genus with 1%increase of relative abundance included Trichoderma(1%),Sordariomycetes(1.5%),Mortierella(1.3%),Penicillium(1%),meanwhile the fungal genus with 1%decrease of relative abundance included Fusarium(30%)and Pleosporales(1%).Conclusion:In summary,T.brevicompactum 31636 can control the root rot of A.macrocephala by regulating of the soil fungal community and inhibiting pathogen.展开更多
基金supported by the National Natural Science Founda-tion of China(Grant No.81872951,82173920)Basic Study on Public Projects in Zhejiang Province(Grant No.LGN21H280002)Zhejiang Provincial Xin Miao Talents Program(Grant No.2020R410057).
文摘Background:Trichoderma brevicompactum can control of plant diseases,but there are few studies about the control of root rot by T.brevicompactum 31636 and its biocontrol mechanism.Objective:To explore the impact of T.brevicompactum 31636 on root rot and the structure of fungal communities of rhizosphere soil.Methods:The effect of T.brevicompactum 31636 on controlling root rot was assessed,the growth of plants was observed by pot experiments,and the fungal structure was detected by high-throughput sequencing analysis.Results:Compared with the CK(healthy plants)group,the plant height,fresh weight,and dry weight increased by 55.3%,48.55%and 57.8%,respectively.While the concentration of atractylenolideⅠ,Ⅱ,Ⅲ,and atractylone increased by 19.35%,16.96%,89.42%and 89.32%,respectively.The results of pot experiments indicated that T.brevicompactum 31636 could significantly improve the survival rate of Atractylodes macrocephala.The results of principal coordinates analysis(PCoA)showed that the sample of CK and Fo+Th(adding Fusdrium oxysporum before T.brevicompactum 31636)group on one side,and samples of Th and Fo group on the other.Furthermore,among the top 20 dominant fungi detected,the fungal genus with 1%increase of relative abundance included Trichoderma(1%),Sordariomycetes(1.5%),Mortierella(1.3%),Penicillium(1%),meanwhile the fungal genus with 1%decrease of relative abundance included Fusarium(30%)and Pleosporales(1%).Conclusion:In summary,T.brevicompactum 31636 can control the root rot of A.macrocephala by regulating of the soil fungal community and inhibiting pathogen.
